Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,316 G→C 5.6% E333Q (GAA→CAA)  EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,3160GC 5.6% 1271.1 / 8.5 532E333Q (GAA→CAA) EO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (236/229);  new base C (3/38);  total (241/291)
Fisher's exact test for biased strand distribution p-value = 1.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 532 total aligned reads displayed.

AGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGGTG  >  NZ_CP034943/3584180‑3584454
                                                                                                                                        |                                                                                                                                          
                aaTAAAACAAGCTCCAAAAGATTAGTAAAAGAGAATTCATAAATGCCTTTACAAGCAGAGCACGCATTTACTCAAGTAGCAACTGCTGAATTATCGTAAAACAACCACATTACACCCAACCAAATAAAAATTCTAAGAAt                                                                                                                         >  2:2331802/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAACTGAGAATGGATTAATGCCTTTACAATCCGAGCACGCATATCATCAAGTAGCAACTGATGATTTATCGTTAAACCAGCCCATGACAACCAACGAAATAAAAAGTCAAAGAAt                                                                                                                         >  2:2763518/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                                                     <  1:1050524/128‑1 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTaataat                                                                                                                         >  1:2502550/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         <  2:413858/140‑1 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:3610157/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:2005471/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:1093958/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACACAACGAAATAAAAAGTCTAAGAAt                                                                                                                         >  2:647939/1‑140 (MQ=255)
                aaTAAAACAAGCTCCAAAAGAGTCCTACATGAGACAGCATTAATGCATATACAACCCCAGGACGCATAAACTCAAGAAGAAACTGCTGATATATCGTTAAACCATACAAATACAACCAAAGAAATAAAAAAACTATGAAt                                                                                                                         >  2:4675501/1‑140 (MQ=255)
                     aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    >  1:1368418/1‑140 (MQ=255)
                     aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    >  1:3114722/1‑140 (MQ=255)
                     aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                                                    >  1:100827/1‑140 (MQ=255)
                        caGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATACAAAGTCTAAGAATTGCGATTg                                                                                                                 <  1:3084928/139‑1 (MQ=255)
                        aaGCTCCAAAAGCGGCGGAAATGAGAATGCAATAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                                                 <  1:82431/140‑1 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGACATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  1:1781084/140‑1 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  2:1581911/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:630206/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  2:881350/140‑1 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:4460014/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:4074667/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               <  1:3724128/140‑1 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  2:4587028/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:2927120/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                                                               >  1:1863322/1‑140 (MQ=255)
                          gCTCCAAAAGATGCGGAAAGGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAATGCGATTgaa                                                                                                               >  1:2178867/1‑138 (MQ=255)
                            tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                                             <  1:3942082/140‑1 (MQ=255)
                            tCCAAAAGATGCGGAAATGAGAATGCATTAATGCCATTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGt                                                                                                             <  1:4779129/140‑1 (MQ=255)
                             ccAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTg                                                                                                            <  1:3625417/140‑1 (MQ=255)
                               aaaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGat                                                                                                          >  2:2695724/1‑140 (MQ=255)
                               aaaaGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGat                                                                                                          >  2:4797378/1‑140 (MQ=255)
                                      gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                                                   >  1:4347640/1‑140 (MQ=255)
                                                          aTGCATTTACAACCCGCGGCCGCATTTCCTCAATTCCCAACGGGTTATTTATCGTAAAACCACCCAATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTCCAGTTATATGTAGGCTAAAATCGACTCGTCTGcc                                                                               <  1:4023488/140‑1 (MQ=255)
                                                               tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTCAACTGATATGTAGGAAAAAATCTACTAGTCTGCaaaaaa                                                                          >  2:2928383/1‑140 (MQ=255)
                                                                ttACAGGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATTTATGCTAAAATCGACTCGTCTGCCGaaaaa                                                                         >  2:3958756/1‑140 (MQ=255)
                                                                       ccGAGCACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                                                  <  2:1301378/140‑1 (MQ=255)
                                                                        cGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                                                 >  2:1126458/1‑140 (MQ=255)
                                                                          agcaCGCATTTCCTAAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                                                               <  2:4463542/137‑1 (MQ=255)
                                                                          aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGATAAAATCTACTAGTCTGACGAAAAAAGCGCTGCTc                                                               >  2:2572271/1‑140 (MQ=255)
                                                                          aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTCCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAATCGCTGCTc                                                               >  2:2109944/1‑140 (MQ=255)
                                                                           ggACGAATTTCCACAATTAGAAACTGCTGATTTATCTTTAAACAAGCCATTTACCCCCAACCAAAAAAAAAGTCTAACAATTGCGATTGCAGTGTTATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  1:2422045/140‑1 (MQ=255)
                                                                             actcATTTACTCACTTAGCAACTGCTTATTTTTCGTTAAACCAGCCCATTACACCCAACGAATTAAAAAGTCTAAGAATTGCTATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCt                                                            <  2:3032158/137‑1 (MQ=255)
                                                                               gaaTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  2:2549816/138‑1 (MQ=255)
                                                                               gaaTTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  2:765187/138‑1 (MQ=255)
                                                                               gCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAACTAAAAAGTCTAAGAATTGCGATTCCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  2:2072828/140‑1 (MQ=255)
                                                                               gCATTTCCTCAAGTAGAAACTGCTTTTTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCTGTGATATGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  2:489987/140‑1 (MQ=255)
                                                                               gCATTTCATCAATTAGCATCTTTTTATTTATCGTTAAACCAGCCCATTCCACCAAACGAAATAAAAAGTCTAATATTTGCGATGGCTTTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  1:197487/140‑1 (MQ=255)
                                                                               ccagttCCTCAAGTAGCAACTGCTCATTTATCTTTAAACCCCCCAATTCCACCCAACGACAGAAAACTTCTAATAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                                                          <  1:2826938/136‑1 (MQ=255)
                                                                                  tttCCTCATGTAGCAACTGCTTATTTATCGTTAAACCAGCCAATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATTTGTTGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  2:575760/140‑1 (MQ=255)
                                                                                  tttCCTCAATTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACTAAATAAAACTTCTAATAATTGCGATTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  2:4714159/140‑1 (MQ=255)
                                                                                  tttCCTCAATTAGCAACTGATGATTTATCGTTAAACCAGCCCTTTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  2:3673444/140‑1 (MQ=255)
                                                                                  tttCCTCAAGTAGAAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCTATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:1502514/140‑1 (MQ=255)
                                                                                  tttCCTAAATTAGAAATTTTTTATTTATCTTTAAACCAGCCAATTACACCCAACTAAATAAAAAGTATAAGATTTGCGTTTGCAGTGATTTGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                                                       <  1:2695724/140‑1 (MQ=255)
                                                                                   ttCCTAAAGTAGCAACGGCTGATTTATCTTTAAACCCGCCCATCACACCCACGGAAATAAAAAGTCTACGAATGGCGTTTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                                      <  1:1912306/140‑1 (MQ=255)
                                                                                   ttCATCAAGTAGCAACTGGTGATTTATCGTTAAACCAGCCAATTACCCCCACCGAAATAAAAAGTCTACGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                                                      <  1:4858614/140‑1 (MQ=255)
                                                                                    tCCTCAAGTAGCAACTGGTTATTTATCTTTAAACCACCCAATTACACCCACCGAAATAAAAAGTCTAAGAATTGCGTTTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACg                                                     <  2:4423074/140‑1 (MQ=255)
                                                                                    tCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCACCCCATTACACCCACCCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACg                                                     <  2:1719797/140‑1 (MQ=255)
                                                                                        cAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGtat                                                 <  2:1410470/140‑1 (MQ=255)
                                                                                        cAAGTAGAAACTGCTGATTTATCGTTAAACCAGCCCATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCGGCTCCCTATTTACGGtat                                                 <  1:1196485/140‑1 (MQ=255)
                                                                                           gTAGCAACTGCTGATTTATCGTTAAACCCCCCCATTACACCCAACTAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                                              <  2:3403464/140‑1 (MQ=255)
                                                                                           gTAGCAACTGCTGATTTATCGTTAAACCCCCCCATAACACCCAACGAAATAAAACGTCTAATAATTGCGTTTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                                              <  1:3272447/140‑1 (MQ=255)
                                                                                           gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                                                   >  1:2986386/1‑135 (MQ=255)
                                                                                           gTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGt                                                   <  2:3109629/135‑1 (MQ=255)
                                                                                             aGCAACTGATCTTTTATCGATAAACCCCCCCATTACACCCAACCAAATAAAAAGAATAATAATTGCTTTTGCAGTGTTATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt                                            <  1:4139954/140‑1 (MQ=255)
                                                                                               cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCt                                          >  2:3151849/1‑140 (MQ=255)
                                                                                                aaCTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTg                                         >  2:1795582/1‑140 (MQ=255)
                                                                                                 aCTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGt                                        >  1:920307/1‑140 (MQ=255)
                                                                                                     cttaTTTATCTTTAAACCACCCCATAACACCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa                                    <  1:716483/137‑1 (MQ=255)
                                                                                                     cttaTTTATCGTTAAACCAGCCCATCACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa                                    <  1:1098894/137‑1 (MQ=255)
                                                                                                     ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa                                    >  2:1842992/1‑140 (MQ=255)
                                                                                                      ttaTTTATCGTTAACCCCGCCCATTACCCCCAACTAAATAAAAAGACTAATAATTGCGATTGCATGGATAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                                   <  1:3170634/138‑1 (MQ=255)
                                                                                                      tgATTTATCGTTAAACCCGCCCATTACACCCACCGAAATAAAAAGTCTACGAAGTGCGATTGCAGTGAAAGGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                                   <  1:863611/140‑1 (MQ=255)
                                                                                                      tgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATGGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAAc                                   <  1:2080442/140‑1 (MQ=255)
                                                                                                       gATTTATCGTTAAACCAGCACATTACCCCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACg                                  <  2:1202435/140‑1 (MQ=255)
                                                                                                           tATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGAAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGt                              <  1:2586496/140‑1 (MQ=255)
                                                                                                             tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                                                              <  2:1498639/106‑1 (MQ=255)
                                                                                                             tCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                                              >  1:4264914/1‑122 (MQ=255)
                                                                                                                 tAAACCAGCCAATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATc                        <  1:4560253/140‑1 (MQ=255)
                                                                                                                  aaaCCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTCTTTACGGTATACCATCTGATGAACGCTGTTTAATCa                       >  2:853709/1‑140 (MQ=255)
                                                                                                                       aGCCCTTTACCCCCAACCAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGc                  <  2:2163073/140‑1 (MQ=255)
                                                                                                                        gCCCATTACACCCCACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTccc                 >  2:4058418/1‑140 (MQ=255)
                                                                                                                        gCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTAAACCATATGTTGAACGCTGTTTAATCAATTgac                 >  2:2857068/1‑138 (MQ=255)
                                                                                                                           cATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCTGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTgg              <  2:3829317/140‑1 (MQ=255)
                                                                                                                            atgccacCCCACGAACTAAAAAGTCTAACAATTGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  2:3196381/136‑1 (MQ=255)
                                                                                                                            aTTACACCAAACTAAATAACAAGTCTACGAATTGCGATTGCAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGc             <  2:1873008/140‑1 (MQ=255)
                                                                                                                             ttACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                                                         >  2:4248287/1‑95 (MQ=255)
                                                                                                                              tACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTg           >  2:244748/1‑140 (MQ=255)
                                                                                                                              tACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATAAATTGCCTGGCTg           >  2:1043235/1‑140 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCTGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                                              <  1:3351092/100‑1 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc                                              >  2:3351092/1‑100 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCt      >  1:1611072/1‑140 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCt      >  1:4594353/1‑140 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATGTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCt      >  1:2120416/1‑140 (MQ=255)
                                                                                                                                   ccAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACACGTCAGCCGAAAAAAGCGCTGCTCCCTAATTACGGTATACCATCTGTTTAACTCTGTTTAATCAATTGCCTGGCTGTCCCt      >  1:3662002/1‑140 (MQ=255)
                                                                                                                                     aaCGAAATAAAAAGTCTCAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTGACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  1:3223740/1‑140 (MQ=255)
                                                                                                                                     aaCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  1:1458460/1‑140 (MQ=255)
                                                                                                                                     aaCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTgg    >  1:2737879/1‑140 (MQ=255)
                                                                                                                                      aCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTCTTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGgt   >  2:2253924/1‑140 (MQ=255)
                                                                                                                                      aCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGgt   >  1:4635075/1‑140 (MQ=255)
                                                                                                                                       cGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGgtg  >  1:1693189/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACGCTGTTTAATCAATTGCCTGGCTGTCCCTGGTG  >  NZ_CP034943/3584180‑3584454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: