BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,719,864	T→G	7.0%	S79R (AGC→CGC)	EO946_RS19145 ←	YwdI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,719,864	0	T	G	7.0%	934.5 / 14.6	422	S79R (AGC→CGC)	EO946_RS19145	YwdI family protein
Reads supporting (aligned to +/- strand): ref base T (172/204); new base G (3/43); total (175/247)
Fisher's exact test for biased strand distribution p-value = 4.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 427 total aligned reads displayed.

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

aaCGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCt                                                                                                                                           >  1:947262/1‑140 (MQ=255)

  cGCTGTCCATTGCATCGCACCAAGAACCAATTTCACATATCTCAACCTTTATCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGGTTTCGATATTTGTTTAATCCCGCGCTTc                                                                                                                                         >  2:2482276/1‑140 (MQ=255)

  cGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTc                                                                                                                                         >  2:4966650/1‑140 (MQ=255)

  cGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTc                                                                                                                                         >  2:4555417/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  1:3227592/1‑140 (MQ=255)

       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:1962183/1‑140 (MQ=255)

       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGATTTCGATATTTGTTTAGTCACGCGCTTCCcagc                                                                                                                                    >  2:16912/1‑138 (MQ=255)

            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCACGCGCTTCCCAACAtttt                                                                                                                               >  1:4970398/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGGTTCCCAACATTTTTTCca                                                                                                                          >  1:3890688/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttttt                                                                                                                             >  1:4139864/1‑137 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:4244057/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:78579/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:1074766/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:3834264/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCca                                                                                                                          >  2:2695287/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:1806310/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGCTATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:1856105/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTTCCCATCAATTTTTAca                                                                                                                          >  1:1162017/1‑140 (MQ=255)

                   cACCAAGAACCAATTTCACATATCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCACATCATTTTATCcatc                                                                                                                        >  2:3491870/1‑140 (MQ=255)

                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  2:1473379/1‑140 (MQ=255)

                        aGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAg                                                                                                                   <  2:2323530/140‑1 (MQ=255)

                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  1:1601815/140‑1 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTACAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  1:3018148/1‑140 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  2:4414711/1‑140 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  1:2940703/1‑140 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  1:3096006/1‑140 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATACCGCGCTTCCCATCAGTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  2:4470687/1‑140 (MQ=255)

                                    acaTAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGa                                                                                                       <  1:1312916/140‑1 (MQ=255)

                                       tcgCTCACCCTTTCTCATTAAAAATCAAATCCCGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:4111552/138‑1 (MQ=255)

                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAACGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  1:2742222/140‑1 (MQ=255)

                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  1:1750804/140‑1 (MQ=255)

                                         gCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCg                                                                                                  <  1:1211875/140‑1 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  2:3899944/1‑140 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  2:2231024/1‑140 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  2:4014754/140‑1 (MQ=255)

                                               ccTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              >  2:1739873/1‑122 (MQ=255)

                                                   tctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTAATGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCAATCATCAGCTCGTCTGTCGATGGCGGTGCGGCAgg                                                                                        >  2:3320575/1‑140 (MQ=255)

                                                   gctcATTAAAAATCAAATAAAGACTCTCCGTGTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAgg                                                                                        <  2:2903577/139‑1 (MQ=255)

                                                       aTTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTGCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCGATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTc                                                                                    >  1:2770412/1‑140 (MQ=255)

                                                         tAAAAATCAAATAAAGTCTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCAGCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCgg                                                                                  >  2:3451331/1‑140 (MQ=255)

                                                         tAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTTTTTAATCCCCCGCGTCCCATCAATTTTTCCATCATAAGCTGGTCTGTCGATTGCGGTGCGGCAGGACTCgg                                                                                  >  1:3174972/1‑140 (MQ=255)

                                                                  actAAAGCCTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:2528000/138‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATCTTTGTTTAATCCCGCGCGTCCCCTCATTTTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:1865012/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:4460997/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:4754006/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:4247431/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4323153/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2640670/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:729793/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:2126815/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:3581295/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:1202958/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:222493/140‑1 (MQ=255)

                                                                  aaTAAACACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  1:1397753/140‑1 (MQ=255)

                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  2:898961/140‑1 (MQ=255)

                                                                       aGACACTCCGGTTCCGTCCTCTTCCTGCTTCTCTTGCTCCTGCGTTCGATATTGGTTTAATCCCGCGCTTCCCATCAGTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCt                                                                    <  2:711675/140‑1 (MQ=255)

                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  1:1712543/122‑1 (MQ=255)

                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:2044114/1‑140 (MQ=255)

                                                                                  ttCCGACGTCTTCCTGCTTCTCTTTCTCCTGCGTTCGATCTTTGTGTAATCCCGCGCTGCCCATCATTTGTTCCCTCATGAGCTCGGATCTCGAGGGCTGTGCGGAAGGACTCGGCGAGCTGCTCGGCGGTATGCGGGgc                                                         >  2:859498/1‑140 (MQ=255)

                                                                                   tCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGgcg                                                        <  2:2145669/140‑1 (MQ=255)

                                                                                     cGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCATTCGATTGCGGTTCGGCAGGGCGCGGCGTGCTGCTCGGCGGTATGCGGGgcgcc                                                      >  1:3953648/1‑139 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAg                                                                                                                   <  1:4049811/77‑1 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:4639383/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:1822909/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:4306629/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTCTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGc                                                                                           >  1:3500895/1‑101 (MQ=255)

                                                                                         ctctTCCTGCTTCTCTGTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:2459487/140‑1 (MQ=255)

                                                                                           ctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcgcg                                                >  2:1922116/1‑140 (MQ=255)

                                                                                             tCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGt                                              <  2:3113701/140‑1 (MQ=255)

                                                                                               cagcgtctcGTACTCCCCCTTTCGAGATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCGGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                            <  2:4166439/135‑1 (MQ=255)

                                                                                                   ttCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCgg                                        <  2:3826170/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCTCTGCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  2:2085482/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCTTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCgtc                                       <  2:3724488/140‑3 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:4931846/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       >  1:1017604/1‑140 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:3617968/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       >  2:1156868/1‑140 (MQ=255)

                                                                                                      tctTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTg                                     >  1:600893/1‑140 (MQ=255)

                                                                                                             ccTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGc                              >  1:2881229/1‑140 (MQ=255)

                                                                                                              ctgctgTCGCTATTGGGTTAATCCCGCGCTGCCCATCATTTTGTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:2770412/140‑1 (MQ=255)

                                                                                                              cggCTTTCGAGCTTTGTTTAATCCCGCGCGTCCCATCATAATTTCCAACATCAGCGGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCGGCTCGGCTGTATGCGGGGCGCGCTCGCGGTATCCGGCTGATCCAGCa                             <  2:3679423/138‑1 (MQ=255)

                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           >  1:872668/1‑140 (MQ=255)

                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           >  1:373694/1‑140 (MQ=255)

                                                                                                                gCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           <  2:4285802/140‑1 (MQ=255)

                                                                                                                gCTTTAGATATTTGTTTCATCCCGCGCTTCCCACCCTTTTTTCCAGCATCAGCGGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCGCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCacg                           <  2:1332469/140‑1 (MQ=255)

                                                                                                                 cTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:516618/137‑1 (MQ=255)

                                                                                                                    tCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGt                       <  2:556607/140‑1 (MQ=255)

                                                                                                                          tttGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                 <  1:2629032/140‑1 (MQ=255)

                                                                                                                          tttGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                 <  1:1098447/140‑1 (MQ=255)

                                                                                                                          tttGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                 <  1:344572/140‑1 (MQ=255)

                                                                                                                           ttGGTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCCGGACTCGGCGTGCTGCTCGGGTGTATGCGGGGCGCGCTCGCGGGCGCCGGCTGAGCCAGCACGACGTcacaccg                >  2:647725/1‑138 (MQ=255)

                                                                                                                            tGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACaaa               <  2:1594312/140‑1 (MQ=255)

                                                                                                                               ttAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGaa            <  1:1243745/140‑1 (MQ=255)

                                                                                                                                tAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAAc           >  1:1511360/1‑140 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAGAGAACGGAc       <  2:3206925/140‑1 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTGTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:550215/140‑1 (MQ=255)

                                                                                                                                      cgcgcgTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACaa     <  2:2941267/140‑1 (MQ=255)

                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:2292931/1‑140 (MQ=255)

                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:681779/1‑140 (MQ=255)

                                                                                                                                         gcTTCCCATCATTTTTTCCATCATCAGCGGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCg  <  2:4779862/140‑1 (MQ=255)

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`