BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,207	G→T	12.9%	Q109K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,207	0	G	T	12.9%	669.9 / 24.8	328	Q109K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (124/150); new base T (52/2); total (176/152)
Fisher's exact test for biased strand distribution p-value = 6.88e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240069‑240345

tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTTGGAGATTTGa                                                                                                                                           >  2:1460255/1‑140 (MQ=255)

 gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACTAAGTCGATCATTCCGACTATACGGGCGTGGTGGGAATTTTTTTTTAAATTTTTTGGGATTTgga                                                                                                                                          >  1:76961/1‑138 (MQ=255)

 gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                          >  2:879626/1‑140 (MQ=255)

 gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                          >  2:2273284/1‑140 (MQ=255)

 ggaaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAAGTTTTGGGCGAGTTgga                                                                                                                                          >  2:2794371/3‑138 (MQ=255)

   aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATg                                                                                                                                        >  2:4034008/1‑140 (MQ=255)

    agagagagagAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTTAATTTTTGGGAGATTTTAAttg                                                                                                                                       >  1:1443840/1‑138 (MQ=255)

    agagagagagAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGAATTGAAttg                                                                                                                                       >  1:3208287/1‑138 (MQ=255)

        agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGGttt                                                                                                                                   >  2:242770/1‑140 (MQ=255)

         gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACTGGCGTTTTCGGATTTTTTTTTGAATTTTTTGGAGATTTGAATTGCtgtt                                                                                                                                  >  1:2305646/1‑140 (MQ=255)

         gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGTGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATGGCtttt                                                                                                                                  >  2:4380277/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGCtttta                                                                                                                                 >  1:4433971/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTTGGAGATTTTAATGGCTTTTc                                                                                                                                 >  1:4778180/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGTGATTTGAATTGGTTTTc                                                                                                                                 >  1:4971073/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATGGCTTTTc                                                                                                                                 >  2:791216/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATATGAATGGCtttta                                                                                                                                 >  2:3472006/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                                 >  1:1194618/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  2:1161323/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTTGGAGATTTGTATTGCTTTTc                                                                                                                                 >  1:36371/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGGGGGATTTGAATGGGTTTTc                                                                                                                                 >  1:1007400/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTATTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                                 >  1:1886764/1‑140 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAGTTTTGGGAGATTTGTTTGGGttttg                                                                                                                                 >  1:3428443/1‑139 (MQ=255)

          agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  1:4359722/1‑140 (MQ=255)

                      tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGGTTTCCGTTTTTAAAAc                                                                                                                     >  2:3068745/1‑140 (MQ=255)

                       ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                    <  1:4693899/140‑1 (MQ=255)

                            aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAATTTTTTGGAGATTTGAATGTCTTTTACGTTTTTAAAACAATAgg                                                                                                               >  2:1762967/1‑140 (MQ=255)

                            aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGTAGTTTTGTGAGATTTTAATTGGTTTTGCCTTTTTAAAACAATAgg                                                                                                               >  1:1142609/1‑140 (MQ=255)

                                tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                           <  1:632275/140‑1 (MQ=255)

                                tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                           <  1:2368403/140‑1 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTCATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGGGTTTCCGTTTTTGAAAAAAGAGGCaaac                                                                                                          >  1:83272/1‑138 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAAGTTTTTGGAGATTTTAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  1:3165810/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATGGCTTTTACGTTTTTTAAAAAATAGGCaatc                                                                                                          >  1:295594/1‑138 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  2:3038114/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTAGGTTTGGGAGATTTGAATTGCTTTTCCGTTTTTTAAAGAATAGGAAAGc                                                                                                          >  2:4075554/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  1:3598034/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGc                                                                                                          >  2:203917/1‑140 (MQ=255)

                                 ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  2:1642429/1‑140 (MQ=255)

                                       attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTGTATGTCTTTTCCGTTTTTTAAAAAAGAGGCAAGCCAGATa                                                                                                    >  1:1511972/1‑140 (MQ=255)

                                        ttCAGTGATGCTGTTTTTGTATTTTTGTTCTAATAAGAAGTCGATCATTCCGACTATACTGGCGTTTTGGGATTTTTGTTTGAATGTTTGGGAGATTTGAATTGCTTTTGAGTTTTTAAAAGAAGAGGCAAGCCCGATaa                                                                                                   >  2:4945662/1‑140 (MQ=255)

                                        ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                                                   >  2:1179482/1‑140 (MQ=255)

                                        ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                                                   >  2:634900/1‑140 (MQ=255)

                                             agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAACCCAGATAAAcccc                                                                                              >  1:2570496/1‑139 (MQ=255)

                                               aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTTAATGGCTTTTGCGTTTTTAAAACAATAGGCAAACCACATAAAACCCac                                                                                            >  1:4064296/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGTTTTTTGTTTGAATTTTTTGGAGATATGAATGGCTTTTAAGTTTTTTAAAAAACAGGCAAGCCAGATAAAACCCacaac                                                                                         >  1:2888273/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAATTTTTTGGCGAATTTAATTGGTTTTACGTTTTTAAAACAAAAGGCCAACCAGATAAACCCCacaac                                                                                         >  1:4491677/1‑140 (MQ=255)

                                                  cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGAATTTTTTTTGAAGTTTTGGGAGATTTGAAATGCGTTTACATTTTTAAAAAAAGAGGCAAGCCACATAAAACCGaaaaa                                                                                         >  1:2046654/1‑139 (MQ=255)

                                                     tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTTAAAAAAGAGGCAAACCAGATAAAACCCacaaaaaa                                                                                      >  1:2639173/1‑139 (MQ=255)

                                                          gTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACaaaaa                                                                                 >  2:1789242/1‑140 (MQ=255)

                                                               tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                            >  2:2196856/1‑140 (MQ=255)

                                                                   ttCTAATACGAAGTCGATCATTCCGACTATACGGGAGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  2:3796902/140‑1 (MQ=255)

                                                                   gtCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:4119117/139‑1 (MQ=255)

                                                                       aaTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAg                                                                    <  2:2577485/140‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  1:3072604/140‑1 (MQ=255)

                                                                        aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  1:1731391/140‑1 (MQ=255)

                                                                         aaCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGCGAGATTTGAATGGCTTTTCCGTTTTTAAAACCAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  1:864005/139‑1 (MQ=255)

                                                                          aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                                                 <  2:4185852/140‑1 (MQ=255)

                                                                               gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  1:1676503/140‑1 (MQ=255)

                                                                               gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  1:1460255/140‑1 (MQ=255)

                                                                                tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGCGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAATAATCTAGCAACTAAAcaaca                                                           >  2:1318881/1‑136 (MQ=255)

                                                                                   aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                        >  2:2449039/1‑140 (MQ=255)

                                                                                   aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                        <  2:1533823/140‑1 (MQ=255)

                                                                                    tccttCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTGGAATGGCTTTTCCGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:2526183/137‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:2449620/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3783697/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:2669889/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:515550/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3003490/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTGTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3880921/140‑1 (MQ=255)

                                                                                    tCATTCCGACTATACGGGAGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:2139935/140‑1 (MQ=255)

                                                                                        tCCGACTATACGGGCGTTTTGGGGTTTTTTTTTGTAGTTTTTGGGGATTTTTATTGGTTTTGCGTTTTTTAAAAAATAGGCAAGCCACATAAAACCGACAACAACAAAAAATAAAATATCAAATAAAAACCAAGCAgggg                                                   >  1:1305270/1‑140 (MQ=255)

                                                                                        tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                                   <  1:1642429/140‑1 (MQ=255)

                                                                                         ccGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGCGATTTTAATTGGGTTTGCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCCACAACAACAAAAAATAAACAAGCAACTAAACACCATGCCAgggg                                                  >  2:3771496/1‑140 (MQ=255)

                                                                                          cGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGCGATTTTAATTGCTTTTACGTTTTTAAAAAAAGATGCAAGCCACATAAACCCGACCCCAACAAAAAATAATATAGCCAGTAAACTCCAGGCATGGGGt                                                 >  1:918730/1‑140 (MQ=255)

                                                                                                taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattca                                           >  2:424615/1‑140 (MQ=255)

                                                                                                     gCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAACCCGACAACAACAAAAAATTATCTAGCAAGTAAACTCCATTCATTGGGTAATTCATTCGt                                      >  2:4851115/1‑140 (MQ=255)

                                                                                                        ttttCGGATTTTTGTTTGTAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGc                                   >  2:4962177/1‑140 (MQ=255)

                                                                                                        ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTGCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAAAAAAAAATAATCTATCAAGTAAAATACATTCATGGGGTAATTGATTAGTTga                                   >  2:2915315/1‑139 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  2:3428443/140‑1 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  2:3626259/140‑1 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:331411/140‑1 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 >  2:3292123/1‑140 (MQ=255)

                                                                                                          ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:4221024/140‑1 (MQ=255)

                                                                                                          ttCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                                 <  1:2236815/140‑1 (MQ=255)

                                                                                                              gATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAActa                             <  2:739453/140‑1 (MQ=255)

                                                                                                                   ttggttgAAGGTTTGGGAGATGTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAACCCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAAGTCATTCGTTGCAAAATATaaaa                        <  2:2645995/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGTGAGATTTTAATGGATTTTCCGTTTTTAAAACAAGAGTCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAATTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:3940320/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:2617896/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:2758577/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:1763316/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:1611802/140‑1 (MQ=255)

                                                                                                                     gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt                      <  1:407992/140‑1 (MQ=255)

                                                                                                                       ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag                    <  2:1093471/140‑1 (MQ=255)

                                                                                                                          aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:2707224/140‑1 (MQ=255)

                                                                                                                          aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCAGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  1:4666938/140‑1 (MQ=255)

                                                                                                                          aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGACAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:3692515/140‑1 (MQ=255)

                                                                                                                          aaGTTTTGGGAGATTTGAATGGATTTTCAGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTATCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:4645695/140‑1 (MQ=255)

                                                                                                                               ttGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTa            <  1:1383673/140‑1 (MQ=255)

                                                                                                                                 gggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt          <  2:2305646/140‑1 (MQ=255)

                                                                                                                                 gggAGATTTGAATGGCTTTTAAGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt          <  1:1073931/140‑1 (MQ=255)

                                                                                                                                  ggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtg         <  1:3679505/140‑1 (MQ=255)

TGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240069‑240345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`