BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	9.0%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	9.0%	902.5 / 12.9	388	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (240/90); new base T (17/37); total (259/129)
Fisher's exact test for biased strand distribution p-value = 1.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 391 total aligned reads displayed.

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851101‑3851376

tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                          <  1:1306443/140‑1 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  1:4180354/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  1:1880349/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  1:2756046/1‑140 (MQ=255)

  tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTGTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTCTCATGCTTCg                                                                                                                                        >  2:4428619/1‑140 (MQ=255)

    ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGGCAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                      <  1:509378/140‑1 (MQ=255)

     cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  1:2681585/137‑1 (MQ=255)

      aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  1:4157917/140‑1 (MQ=255)

      aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  1:1652328/140‑1 (MQ=255)

       aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                                   >  1:4066593/1‑140 (MQ=255)

           gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                               <  2:1880349/140‑1 (MQ=255)

           gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                               <  2:4237641/140‑1 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTACGCATAACCTGCAAAGTACTCGGACAGGTCTTCTATAATTGAACCAGTCGTTATGACCGGCATCATGATTCGATCGTATGCa                                                                                                                              >  2:3275599/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATAGTATGCa                                                                                                                              >  1:798903/1‑140 (MQ=255)

            ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:2583334/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                               >  2:3720684/1‑134 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                            >  1:1027349/1‑137 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                            <  2:1027349/137‑1 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:2644589/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:213614/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:1152424/1‑140 (MQ=255)

                 tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTCTGCAAGccc                                                                                                                         <  2:4805291/140‑1 (MQ=255)

                 tGTAATATGGATTGGAAATGGAAGTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         <  2:348428/140‑1 (MQ=255)

                 ggTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTCTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:3230653/2‑140 (MQ=255)

                      tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTAGATCGTATGCAAGCCCTTccc                                                                                                                    >  1:703744/1‑140 (MQ=255)

                        tGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaa                                                                                                                  >  1:3463002/1‑140 (MQ=255)

                         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                 >  2:568156/1‑140 (MQ=255)

                         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                 >  2:4911131/1‑140 (MQ=255)

                         ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                 <  1:4809652/140‑1 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCTGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATAATAATTAGATCGTAATAAAGCCCTTACCaaaaa                                                                                                               >  1:4383255/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:332936/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:1381810/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:932461/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:2211244/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:3662037/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:3642170/1‑140 (MQ=255)

                           aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  2:1223521/1‑140 (MQ=255)

                             tGGAAATGTAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACg                                                                                                             >  2:4328842/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAcc                                                                                                             >  1:4497941/1‑139 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                             >  1:2348211/1‑140 (MQ=255)

                             tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCACAAACg                                                                                                             >  1:2797108/1‑140 (MQ=255)

                                    ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAc                                                                                                      >  1:2626273/1‑140 (MQ=255)

                                    ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAc                                                                                                      >  1:3162812/1‑140 (MQ=255)

                                    ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAAc                                                                                                      >  2:3628488/1‑140 (MQ=255)

                                    ggAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCACTTCCCAAAAACGATAAAAc                                                                                                      >  2:4014478/1‑140 (MQ=255)

                                          ctctTTTCGATATTTTTATCACTTTTTAAGCATAACCTTCAATGTACTCGCACAGGTCTTAAATAAATCACCCCGCGGTTATTACCTGTATCATGATGCGATCGTAGCCAAGCCCTACCCAACAACTAAAACACGActct                                                                                                <  2:945369/140‑1 (MQ=255)

                                                tCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCAAAAACGATAAAAAGACTCTGcttct                                                                                          >  2:457680/1‑140 (MQ=255)

                                                    actttttATCACCTTTTAAGAAAACCGTAAAAAGTAATCGCACAGGTCTTCTATAAATCAACCAGGCGTTTTTACCTGTAAATTGATTCGTTGCTAGCAAACCCCTCCCCAAAAACGAAAAAACGACTCTGCTTCTTCaa                                                                                      <  2:4669628/137‑1 (MQ=255)

                                                         ttATCACCTTGTAAGCATACCCTTAAATTTACTCGGACAGTTATTCTATAAATGAACCAGTCGTTATTACCTGTATCTTGATTCTATCGTATGCACGCCCTTCCAAAAACCGATAACACGACTCTGCTTCTTCAAGTTCg                                                                                 <  2:3871155/140‑1 (MQ=255)

                                                           aTCACCTTTTAAGAATAACCAAAAAAGTACTCGCACAGGTCTTATAAAAATCAACCCGTCTTTATTACCTTTATCATTATTCTATCGTATGAAACCCCTTACAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCa                                                                               <  2:3950589/140‑1 (MQ=255)

                                                                 ttttAAGCATAACATTCAATGTACGCGGAATGGTTTTCTATAAATGAACCAGTCTTTATAACCTTTATATTGTTTCTCTGGTATGAACGCCCTTCCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                         <  2:2977447/137‑1 (MQ=255)

                                                                 ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCACAAGACAt                                                                         >  2:1289323/1‑140 (MQ=255)

                                                                 ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACCATAAAACCAAGATGCTTCTTCAAGTTCGCAAcacaa                                                                         >  1:147661/1‑139 (MQ=255)

                                                                  tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                        >  2:784391/1‑140 (MQ=255)

                                                                  tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTAAGCAAGCCCTTCCCAAAAACGATAAAACGAATATGCTTCTTCAAGTTCGCAAGACATa                                                                        >  1:842806/1‑140 (MQ=255)

                                                                      aGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                    >  1:4677934/1‑140 (MQ=255)

                                                                      aGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAGGTTCGCAAGACATaacaa                                                                    >  1:3727652/1‑140 (MQ=255)

                                                                          tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  1:3835090/1‑140 (MQ=255)

                                                                             ccTTCAAAGTACCCGCACCGTTCTTATATAAATCAACCCGTCGTTATTACCTGTATCTTGATTCGATCGTATGAACGCCCTGCCCAAAAAGGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2181853/140‑1 (MQ=255)

                                                                             ccTTAAAAGTACTCGGACTGTTCTTATATAATTTAACATGTCTTTTTTACTTTTATAATGTTTCTATCTTTTCCACGCCCTCCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:1854885/140‑1 (MQ=255)

                                                                             ccTTAAAAGTACTCGGACAGTTCTTATATAAATCAACCAGTCTTTATTACCTGTATCATGTTTCGATCGTAGGCACGCCCTGCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:3310339/140‑1 (MQ=255)

                                                                             ccTACAAAGTACTCGGACTGTTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:3153463/140‑1 (MQ=255)

                                                                             ccTACAAAGTACTCGGACAGGTATTATATAATTGAACCAGGCTTTATTACCTGTATCATGATTCTATCGTATCCACGCCCGCCCCAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:4064863/140‑1 (MQ=255)

                                                                             cattaaaaTTACTCGGACTGTTCTTCTATAAATCAACCAGTCTTTATTACCTGTATCATGATTCTAGCGGATGCACCCCCTACCAAAAAACCAAACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:719188/135‑1 (MQ=255)

                                                                             acTTAAAATTACTGGGACTGTTTTTATATAAATCAACCATTCGTTATTACCTTTATATTGATTCTATCTTTTGCACGCCCTCCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:1582577/139‑1 (MQ=255)

                                                                              cTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTtgttga                                                            >  1:358732/1‑140 (MQ=255)

                                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCACGACATAACAACTTGATGAGGCt                                                        >  2:4114484/1‑140 (MQ=255)

                                                                                  aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAATATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                        >  1:1075050/1‑140 (MQ=255)

                                                                                       actctcaCAGTTATTCTAAAAATCAACCAGTCTTTATTACTTGTATAATGATTCGATCGTATGAAAGCCCTACCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAaga                                                   <  2:1065946/134‑1 (MQ=255)

                                                                                       aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGGTAAAACGACTCTGCTTCTTAAAGTTCGCCAGACATAACAAATTGATTAGGCTTAaga                                                   >  1:1722457/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGAAATAACAACTTGATGATGCTTAagag                                                  >  2:2931698/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  2:4464445/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  2:1382429/1‑140 (MQ=255)

                                                                                        cTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:657091/1‑140 (MQ=255)

                                                                                         tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                                 >  2:1740489/1‑140 (MQ=255)

                                                                                         tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                                 >  2:3392175/1‑140 (MQ=255)

                                                                                            gACATGTCTTCTATAAATGAACCCGTCGTTATTACCTTTATCATTATTCGATCGTATGCAAGCCCTGCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  2:115649/140‑1 (MQ=255)

                                                                                            gACAGGTATTAAATAAATCAACCAGTCTTTATTACGTGTATCATGATTCGCCCGCACGCACGCCCTACCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  2:899772/140‑1 (MQ=255)

                                                                                                ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                          >  1:2302376/1‑140 (MQ=255)

                                                                                                     tctATAAATCACCCAGTCGTTATTCCGTGTATCTTGTTTCCATGGTAGGCACGCCCTTCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:358732/140‑1 (MQ=255)

                                                                                                     tatataAATCACCCTGTCTTTATTACCTTTATATTGATTCGTTGGTATGCAAGCCCTTCCAAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:3759252/138‑1 (MQ=255)

                                                                                                       tataAATCAACCTGTCTTTTTAACCTGTATAATGATTCGATCGTAGGCAAGCCCTCCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   <  2:2495935/140‑1 (MQ=255)

                                                                                                          aaaTCAACCTTTCTTTATTACTTTTAAAATGATTCGATCTTAGGCAAGCCATACCAAAAAACAAAACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:4712635/140‑1 (MQ=255)

                                                                                                          aaaTCAACCAGTAGTTTTTACCTTTATAATTATTCTATCCTAGCCACCCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  1:4937102/140‑1 (MQ=255)

                                                                                                           aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATCATGTCTAAATTGcc                               >  2:3115260/1‑140 (MQ=255)

                                                                                                           aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                               >  1:4239299/1‑140 (MQ=255)

                                                                                                            aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGATGCTTAAGAGCGATCCTGGCTAAATTTCCg                              >  1:2948489/1‑140 (MQ=255)

                                                                                                              caaCCAGTCCTTTTAACCTGTCTCATTATTCGATCGGATCCACGCCCTACCCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  1:1980226/139‑1 (MQ=255)

                                                                                                               aaCCAGTGGTTATCACCTGTATCATTATTCGATCGTATCCAAGCCCTCCCCAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:2334863/140‑1 (MQ=255)

                                                                                                               aaCCAGTCTTTTTTACCTTTATCTTGATTCGATCGTTGGAAACCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:2644589/140‑1 (MQ=255)

                                                                                                               aaCCAGTCTTTTTTACCTGTATCATTATTCGATCGTATGAAAGCCCTTCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:2000035/140‑1 (MQ=255)

                                                                                                                aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                          >  1:573552/1‑140 (MQ=255)

                                                                                                                   aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                       >  1:3927489/1‑140 (MQ=255)

                                                                                                                                tttATCATGATTCGACCGTATCCAAGCCCTTCCCAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt          <  2:3853263/138‑1 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:1821664/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:4896969/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:1420248/1‑140 (MQ=255)

                                                                                                                                  tATCATGATTAGATAGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCTGTTAttt        >  1:943455/1‑140 (MQ=255)

                                                                                                                                       tGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa   >  1:2070423/1‑140 (MQ=255)

                                                                                                                                        gATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaa  >  2:2741879/1‑140 (MQ=255)

TGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851101‑3851376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`