BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,906,067	A→C	14.3%	I42S (ATT→AGT)	EO946_RS20100 ←	DUF3255 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,906,067	0	A	C	14.3%	838.3 / 25.8	423	I42S (ATT→AGT)	EO946_RS20100	DUF3255 family protein
Reads supporting (aligned to +/- strand): ref base A (187/150); new base C (75/2); total (271/152)
Fisher's exact test for biased strand distribution p-value = 9.73e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

aGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCAGCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGGCCATGGGTCCCGGGGTaa                                                                                                                                             <  1:2394608/140‑1 (MQ=255)

     tttGGGAAACCACCTTGTATGCGATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCGTTGGTAAAGGTGACTGTTGCGGATTTCACGCAGTCCATGGGTCCCGGGGTAAGTccc                                                                                                                                        <  1:55004/140‑1 (MQ=255)

     tgtGGGAAACCACCTGGTATGCCATAAAACGCAGTTGCACCTCTCCTCTACCGTCAAGCACTTCCCTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCGGATTTCACGCCGTCCATGGGTCCCGGGGTAATTccc                                                                                                                                        <  2:1871559/138‑1 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTc                                                                                                                                      >  1:3669613/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTc                                                                                                                                      >  1:2810251/1‑140 (MQ=255)

       tGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTc                                                                                                                                      >  1:4234426/1‑140 (MQ=255)

            aaCCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTAATccc                                                                                                                                 >  2:2241396/1‑140 (MQ=255)

                    tGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCg                                                                                                                         <  1:1585086/140‑1 (MQ=255)

                        tGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAAt                                                                                                                     >  1:4966969/1‑140 (MQ=255)

                           cATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCCTTCCCTCATCCCACGTTCCGAAATaaa                                                                                                                  >  1:4796593/1‑140 (MQ=255)

                           cATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATaaa                                                                                                                  >  2:1726254/1‑140 (MQ=255)

                           cATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCAGAAATaaa                                                                                                                  >  2:1611784/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATcaaa                                                                                                                 >  1:2093077/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaaaa                                                                                                                 >  1:467460/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaaaa                                                                                                                 >  1:3855997/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaaaa                                                                                                                 >  2:1003545/1‑140 (MQ=255)

                            aTAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATaaaa                                                                                                                 >  2:2194231/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTc                                                                                                        >  2:3207655/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTc                                                                                                        >  2:75212/1‑140 (MQ=255)

                                     aGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTc                                                                                                        >  1:1186486/1‑140 (MQ=255)

                                      gTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTcc                                                                                                       <  2:3772506/140‑1 (MQ=255)

                                      gTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTcc                                                                                                       <  1:3633805/140‑1 (MQ=255)

                                       ttGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCct                                                                                                      >  1:4022939/1‑140 (MQ=255)

                                         gCACCTCTCCTCTTCCTTCAATCACGTCCGTCTCCACCACCTCGTGGTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCctct                                                                                                    <  2:907484/140‑1 (MQ=255)

                                          cACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTaa                                                                                                                                             >  1:4301475/1‑98 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  1:3064310/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  2:2102262/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  2:3294341/1‑140 (MQ=255)

                                               ctcctctTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTttgt                                                                                              >  2:346998/1‑140 (MQ=255)

                                                     ttccttcAATCCCGTCCGTCTCCACCACCTCGTCTTTGGTACAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAACTAAAAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        <  2:2164930/140‑1 (MQ=255)

                                                     ttccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        <  2:3064310/140‑1 (MQ=255)

                                                     ttccttcAATCACCTCCGTCTCCACCACCTCGTCTTGGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGa                                                                                        <  2:4269516/140‑1 (MQ=255)

                                                      tccttcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGAc                                                                                       <  1:1795578/140‑1 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  2:299526/1‑140 (MQ=255)

                                                          tcAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGcc                                                                                   >  1:4108366/1‑140 (MQ=255)

                                                            aaTCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCg                                                                                 >  2:515958/1‑140 (MQ=255)

                                                             ctCACTTCCGGCCCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATATCACGCCGTCCATGGGTCCCGGGGGAATTCCCTCATCCCACGTTCCGAAATAAAAAGCCATTTCCTCTTTTTGTTGTTGACACCCCGa                                                                                <  1:3181477/139‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:3294071/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:1478423/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:4341646/140‑1 (MQ=255)

                                                               cACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCAATCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  2:2296177/140‑1 (MQ=255)

                                                                 cTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            <  2:4231054/140‑1 (MQ=255)

                                                                 cTTCCGTCTCCACCACCTCGTCGTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAc                                                                            <  2:3572878/140‑1 (MQ=255)

                                                                  ttCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACg                                                                           >  1:3874935/1‑140 (MQ=255)

                                                                    ccGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  2:4717385/140‑1 (MQ=255)

                                                                         tcCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTACACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGca                                                                    <  1:4570639/140‑1 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  1:1139648/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGt                                                                          >  1:2715486/1‑131 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  1:876377/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  2:2413210/1‑140 (MQ=255)

                                                                            accaccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGcacac                                                                 >  2:2030662/1‑140 (MQ=255)

                                                                              caccTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACAcag                                                               <  1:2217221/140‑1 (MQ=255)

                                                                                   cGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGtt                                                          >  2:1197397/1‑140 (MQ=255)

                                                                                     tCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTGCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTaa                                                        >  1:2609409/1‑140 (MQ=255)

                                                                                          ggTAAATGTGACTGTTGCTGATTTCACACCGTCCGTTGGCCCGGGGGCACGCCCCTCATCCCACGTTCCGTAATAAAACGGCGTCTCTTCTTTTTGCTGCTGGCAGCCCGATACCATGATGCATACAGCAATTAAAAACa                                                   >  1:1921863/1‑140 (MQ=255)

                                                                                             aaaTGTGACTGTTGCTGATTTCACACCGTCCGTTGGCCCGGGGGCACGCCCCTCATCCCACGTTCCGTAATAAAACGGCGTCTCTTCTTTTTGCTGCTGGCAGCCCGATACCATGATGCATACAGCAATTAAAAACaaaa                                                <  2:340048/140‑1 (MQ=255)

                                                                                             aaaGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCaaaa                                                <  2:2613676/140‑1 (MQ=255)

                                                                                               aGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAGTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATg                                              >  1:294752/1‑140 (MQ=255)

                                                                                               aGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATg                                              >  2:2817486/1‑140 (MQ=255)

                                                                                                     cTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCCTTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAt                                        >  2:1144805/1‑140 (MQ=255)

                                                                                                       gTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      <  2:1477201/140‑1 (MQ=255)

                                                                                                       gTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAttt                                      <  1:4875816/140‑1 (MQ=255)

                                                                                                         tGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGt                                    <  2:1535979/140‑1 (MQ=255)

                                                                                                           cTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTGTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACAGTTGttt                                  >  2:4928441/1‑140 (MQ=255)

                                                                                                               tttCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAAAGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCa                              <  2:705720/140‑1 (MQ=255)

                                                                                                                ttCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCaa                             >  1:3959958/1‑140 (MQ=255)

                                                                                                                   aCGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  1:128539/140‑1 (MQ=255)

                                                                                                                   aCGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  1:972929/140‑1 (MQ=255)

                                                                                                                   aCGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCAGTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGt                          <  1:346998/140‑1 (MQ=255)

                                                                                                                        gTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCa                     <  1:75212/140‑1 (MQ=255)

                                                                                                                         tCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCAc                    >  2:618394/1‑140 (MQ=255)

                                                                                                                             tGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATc                <  2:4863694/140‑1 (MQ=255)

                                                                                                                             tGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATc                <  2:2360698/140‑1 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         <  1:138953/79‑1 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTg                                                                         >  2:138876/1‑79 (MQ=255)

                                                                                                                                 tCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCttt            <  1:299514/140‑1 (MQ=255)

                                                                                                                                   ccGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:1096175/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:4363445/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCCTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCATTtcc          >  2:536591/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:4912330/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTCATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:198316/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:1734007/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:386569/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTACTTCCCTCATCCCACGGTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:2512737/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACa                                                                                      >  2:2438505/1‑64 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              >  2:4423713/1‑72 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATa                                                                              <  1:4423713/72‑1 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCACGGTGATCACCATCCTTtcc          >  2:4139961/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCa                     <  2:4486568/129‑1 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:2700408/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:1866966/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:427931/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:213644/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcc          >  2:1952639/1‑140 (MQ=255)

                                                                                                                                   ccGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATGTGTTTCTCAAGGTGATCACCATCCTTtcc          >  1:2341808/1‑140 (MQ=255)

                                                                                                                                      gggTAGTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       >  1:3766652/1‑140 (MQ=255)

                                                                                                                                      gggTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTtcctcc       >  1:3587957/1‑140 (MQ=255)

                                                                                                                                           aTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCttt  >  2:3700726/1‑140 (MQ=255)

                                                                                                                                           aTTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCttt  >  2:2911346/1‑140 (MQ=255)

                                                                                                                                           aTTCCCTCATCCCACGTTCCGAAATAAAAAGGCAGGTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCAGACAGCGGGTCAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTGGCCTCCTCttt  >  2:4809324/1‑140 (MQ=255)

AGTACTTTGGGAAACCACCTTGTATGCCATAAAAGGCAGTTGCACCTCTCCTCTTCCTTCAATCACTTCCGTCTCCACCACCTCGTCTTTGGTAAAGGTGACTGTTGCTGATTTCACGCCGTCCATGGGTCCCGGGGTAATTCCCTCATCCCACGTTCCGAAATAAAAAGGCATTTCCTCTTTTTGTTGTTGACAGCCCGATAACGTGATGCACACAGCAGTTAAAAGCAAAATGCCACATTTGTTTCTCAAGGTGATCACCATCCTTTCCTCCTCTTT  >  NZ_CP034943/3905928‑3906206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`