Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 7.1% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 7.1% 651.0 / 5.1 256intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (125/101);  new base A (2/27);  total (127/129)
Fisher's exact test for biased strand distribution p-value = 4.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTC  >  NZ_CP034943/3996718‑3996995
                                                                                                                                           |                                                                                                                                          
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  2:4229095/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  2:2231705/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  1:4654305/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                            >  1:4400027/1‑140 (MQ=255)
      cGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTaaaaa                                                                                                                                      >  1:1320099/1‑140 (MQ=255)
      cGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTaaaaa                                                                                                                                      >  1:4466557/1‑140 (MQ=255)
         ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCt                                                                                                                                   >  2:2357003/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCATTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  2:4659276/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  1:1670731/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  2:253728/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                              <  2:11687/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  1:40659/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  1:3735822/1‑140 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGGTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                         >  2:2987711/1‑140 (MQ=255)
                     caaTCAAGTTGATCAGAGAAGACACAGAAAGGCCATTGTAAATCAGACCAGTAAATTAATGTAAATCACTATTCGTTCGCTTCCATTAGTTTTACAAGCGTTTTCTCAACGCATTGTCCTAAAAAGCTCGTGAAACtttt                                                                                                                       <  1:3306495/139‑1 (MQ=255)
                      aaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTa                                                                                                                      >  2:4047494/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTAGAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                    >  1:3630175/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                    >  2:569606/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                    >  2:201116/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                    >  2:1846888/1‑140 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                    >  1:718551/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                             <  2:2448070/140‑1 (MQ=255)
                                 tCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCAc                                                                                                           >  1:4445422/1‑140 (MQ=255)
                                 tCAGTGAAGAAACAGAAAGTCAAGTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTGCTCATCGCATTGTCATAACAAGCGGGTGAAACATTTATCCtgactcac                                                                                                           >  2:2928114/1‑133 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                       <  1:2725649/140‑1 (MQ=255)
                                     tGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaa                                                                                                       >  1:435754/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAATCTCGTGAAAATTTTATCCTTTAGCACGaaaaa                                                                                                     >  2:3459952/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                     >  2:704812/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                     >  1:2281192/1‑140 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                     >  1:3150116/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTaaaa                                                                                                                                       >  1:3353133/1‑98 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  2:4826681/140‑1 (MQ=255)
                                                  aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                          <  2:4758189/140‑1 (MQ=255)
                                                  aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                          <  2:40659/140‑1 (MQ=255)
                                                      aaTTGGAAATCCGAACATTAAATTACTGTAACTCAATATTCGTTCGCTTCCATTCGTATTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGa                                                                                      <  1:254575/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  2:1526415/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  1:3768027/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  1:4699670/140‑1 (MQ=255)
                                                          gTAAATCAGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATc                                                                                  <  2:2659308/140‑1 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                              >  1:58443/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                              >  1:2445511/1‑140 (MQ=255)
                                                                  gAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGt                                                                          >  1:4025798/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCTTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                         <  2:3562161/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                         <  1:554038/140‑1 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                         >  2:3177188/1‑140 (MQ=255)
                                                                   aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                                         >  2:4548878/1‑140 (MQ=255)
                                                                    aCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTAt                                                                        <  1:4978662/140‑1 (MQ=255)
                                                                        taaattaaTGTAAATCAATATTCCTTCGCTTCCATTAGTTTTACAAGAGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCaa                                                                    >  1:4022362/1‑139 (MQ=255)
                                                                         caattaaTGTAAAGCAATAATCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTGTTAGCCTTTAGCACGAAAAAGAGTTCCTGGCCTGATAGCCGGTTTGTAtgacga                                                                   >  2:3790472/2‑135 (MQ=255)
                                                                              attGTAAATCAATATTCGTTCGCTTCAATTCTTTTTAAACGCTTTTTCTCATCGATTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGAACTAAAAATATTTACTTGCCTTATATCCCTTTTGTATTCATAAAAAc                                                              <  2:4184987/138‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  2:4121980/1‑140 (MQ=255)
                                                                                    aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                        >  1:4790073/1‑140 (MQ=255)
                                                                                        aaTATTCGTTCGCTTCCATTCGTTTTACACGGTTTTTCTCATCGAATTGTCCTAAAAAGCTCGTTAAATTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCaa                                                    <  2:3162102/140‑1 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTa                                                                                               <  1:1865699/95‑1 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                  >  1:1006225/1‑140 (MQ=255)
                                                                                              cgttcgCTCCCATTGTTTTAACAAGGTTTTTCTCATCGCATTGTCATAAAACGCTCGTTAAAATTTTATCCTTTCCCACTAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTcc                                              <  1:3510072/140‑1 (MQ=255)
                                                                                               gttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCg                                             >  2:2032825/1‑140 (MQ=255)
                                                                                               gttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCg                                             >  2:190450/1‑140 (MQ=255)
                                                                                                ttcgGTTCCTTTCTTTTTACATGGTTTTTCTAATCGCTTTGTCATAAAAAGGTCGTGAAACTTTTATCGTTTACCACCAAAAAGTGTTACTTGCCTGATATCCGTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                                            <  1:3847268/140‑1 (MQ=255)
                                                                                                ttcgCTTCCTTTCTTTTTAAAAGCTTTTTCTCATCGCTTTGTCATAAAAAGCTCGTTAAACTTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                            <  2:1739892/140‑1 (MQ=255)
                                                                                                ttcgCTTCCATTGTTTTTACAAGCTTTTTCACATCGCATGGTCATAAAACGCAGGTTAACCTTTTATCCTTTCGCACTAAAAAGAGTTACTGGCATGAAATCCCTTTGTTATACATAAAAACGACTCGCCAACCGTCCgg                                            <  1:4764925/140‑1 (MQ=255)
                                                                                                ttcgCTTCCATTCTTTTTACATGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGATTTACTTGCCTGATATCCCTTTGTTATTCATAAAAACGACTCGCCAACCGTCCgg                                            <  2:1814311/140‑1 (MQ=255)
                                                                                                ttcgCTTCCATTCGTTTTACAAGCTTTTTCTCATCGAATTTTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCgg                                            <  2:2394462/140‑1 (MQ=255)
                                                                                                acagCTTCCTTTCGTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCTTTAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                            <  2:2758919/137‑1 (MQ=255)
                                                                                                acagCTTCAATTCTTTTTAAAAGTTTTTTCTCATGGATTTGTCCTAAAAAGCTGGTTAAACTTTTACCCTTTACCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                                            <  2:2166750/137‑1 (MQ=255)
                                                                                                 tcgTTTCCATTCTTTTTACACGCGTTTGCTCTTCGGATTGTCCTAAAAAGCTGGTGAAACTTTTATCCTTTAGAACTAAAAAGCTTTACTTGCGTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGa                                           <  2:35019/140‑1 (MQ=255)
                                                                                                 tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCGCTTTTGTATTCATAAAAACGAATAGCCAACCGTCCGGa                                           >  2:3727437/1‑140 (MQ=255)
                                                                                                  cgCTTCCTTTGGTTTTACAAGCGTTTACTCATCGCATTGTCCTAAAACGCTCGTTAAACTTTTTTCCTTTAGCACGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                          <  2:4915981/140‑1 (MQ=255)
                                                                                                  cccTTCCATTCGTTTTACAAGCGTTTACTCATCTCTTTTTCCTAAAACGCTCTTTAAAATTTTTTCCTTGAGCACGAAAAAGTTTTACTTGCCTCATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGAt                                          <  2:1490953/138‑1 (MQ=255)
                                                                                                    cTTCCATTCTTTTTACAAGCGTTTTCTCATCGATTTGTCATAAAAAGCTCTTTAAACTTTTATCATTTAGCACCAAAAAGAGTTAGTTGCATTATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATcc                                        <  2:220101/140‑1 (MQ=255)
                                                                                                    cTTCCATTCTTTTTACAAGCGTTTTCTCATCGAATTGTCATAAAAAGCTCGTGAAAGTTTTATCCTTTACCACCAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATcc                                        <  2:3973406/140‑1 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                        >  1:864238/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                        >  2:1666274/1‑140 (MQ=255)
                                                                                                        cATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATACCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATACTCTa                                    >  2:1378982/1‑140 (MQ=255)
                                                                                                            ctttttAAAAGGTTTTTATAATCGCATTGTCCTAAAAAGCTCGTCAAAATTTTATCATTTAGCACTAAAAAGTGTTATTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                                <  2:814483/138‑1 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                             >  1:3220988/1‑140 (MQ=255)
                                                                                                                ttACATGCGTTTTATCAGCGCATTTTACTAAAACGGTGGTTAAATTTTTATCGTTTACCACCAAAAAGTGTTAGTTGCCTGATACCCCTTTTGAATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                            <  1:4201048/140‑1 (MQ=255)
                                                                                                                ttACACGCGTTTTCTCATCGCTTTGTCATAAAAAGCTCGGTAAAGTTTTATACTTTCGCACTAAAAAGATTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                            <  2:3024961/140‑1 (MQ=255)
                                                                                                                ttACAAGCTTTTTCTAATCGCTTTTTCATAAAAATCTCTTGAAACTTTTATCATTTAGCACTAAAAAGTGTTACTTGCGTGATTTCCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                            <  2:676138/140‑1 (MQ=255)
                                                                                                                ttAAAAGCTTTTTATCATCGATTTTTACAAAAAAGATCTTCAAAATTTTATCAATAAGAACGAAAAAGAGTTACTGGCATGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                            <  2:3869980/140‑1 (MQ=255)
                                                                                                                     tgtgTTTTATCATCGCTTTTTCATAAAAAGCTCGTGAACATTTTTTCCTTTAGCACTAAAACTAGTTACTTGCCTTATATCCTTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTcc                       <  2:3393696/137‑1 (MQ=255)
                                                                                                                      gCGTTTTCAAATCGCATTGTCCAAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCGTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  2:2140784/140‑1 (MQ=255)
                                                                                                                      gCGTTTTATCATCTCTTTGTCATAAAAAGCTCGTGAAAGTTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  2:4445422/140‑1 (MQ=255)
                                                                                                                      gCGTTTACTCATCGCATTGTCATAAAAAGCGCGTTAAAATTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTTATATCCTTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  1:4755252/140‑1 (MQ=255)
                                                                                                                       gtttttCTCATGGCTTTGTCATAAAAAGCTGTTTAAATTTTTATCATTTCGCACGAAAATTTTTTAGTTGCTTTATATCCTTTTTTTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  1:277197/138‑1 (MQ=255)
                                                                                                                       ggTTTTCTAATCGAATTGTCAAAAAACGCTCGTAAACATTTTATCCTTTAGCACCAAAAAGAGTTACTTGCCTTATATCCCTTTTGTTTACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  1:2188713/139‑1 (MQ=255)
                                                                                                                       cGTTTTATCATCGCTTTGTCATAAAAAGATCTTTAAACTTTTATCCTTTAGCACTAAAAATATTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:1024633/140‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                   >  2:871003/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                   >  1:4569029/1‑140 (MQ=255)
                                                                                                                           ttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt                 >  2:4347218/1‑140 (MQ=255)
                                                                                                                           ttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt                 >  2:1125656/1‑140 (MQ=255)
                                                                                                                            taacATCCCTTTGTCCTAAAATTATCGTTAAATTTTTTAACTTTAGCACCAAAAACTGTTCAGTCCCTTTTATCCCTTTTTTATACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTc                <  1:2147368/137‑1 (MQ=255)
                                                                                                                                 tCGCTTTTTCCTAAACAGCTCGTGAAACTTTTATCATTTAGCACGAAAAAGTTTTAGTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa           <  1:3026967/140‑1 (MQ=255)
                                                                                                                                 tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa           >  1:2907325/1‑140 (MQ=255)
                                                                                                                                 tCGCATTGTCCTAAAAAGCTCGTGAAACTTGTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATACAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa           >  1:1200812/1‑140 (MQ=255)
                                                                                                                                  cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc          >  1:2005159/1‑140 (MQ=255)
                                                                                                                                         tCCTAAACAGGCCGTGAAAGTTTTCACCTTGACCACGAAAATGTGTTACTTCCCTGATATCCCTTTTTTTTCCAAACACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct   <  1:1605788/140‑1 (MQ=255)
                                                                                                                                         gccTCAAAACCTCTTGAAACTTTTATCCTTCCGCACGAAAAAGAGTTACTTGCCGGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct   <  2:3301833/139‑1 (MQ=255)
                                                                                                                                          ccTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtctc  >  2:4148381/1‑140 (MQ=255)
                                                                                                                                          ccTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtctc  >  2:3627166/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTC  >  NZ_CP034943/3996718‑3996995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: