BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,996,927	C→A	7.2%	M131I (ATG→ATT)	EO946_RS20530 ←	VOC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,996,927	0	C	A	7.2%	793.3 / 7.3	343	M131I (ATG→ATT)	EO946_RS20530	VOC family protein
Reads supporting (aligned to +/- strand): ref base C (163/141); new base A (2/37); total (165/178)
Fisher's exact test for biased strand distribution p-value = 1.60e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

TAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGCA  >  NZ_CP034943/3996790‑3997066

taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCAt                                                                                                                                           >  1:4023650/1‑140 (MQ=255)

        tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                                   >  2:3002462/1‑140 (MQ=255)

        tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                                                                                                   >  1:1814311/1‑140 (MQ=255)

            aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCg                                                                                                                               >  1:4790073/1‑140 (MQ=255)

                aaTATTCGTTCGCTTCCATTCGTTTTACACGGTTTTTCTCATCGAATTGTCCTAAAAAGCTCGTTAAATTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCaa                                                                                                                           <  2:3162102/140‑1 (MQ=255)

                  tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                                                                                         >  1:3973406/1‑140 (MQ=255)

                  tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAAcc                                                                                                                         >  2:4090087/1‑140 (MQ=255)

                      cgttcgCTCCCATTGTTTTAACAAGGTTTTTCTCATCGCATTGTCATAAAACGCTCGTTAAAATTTTATCCTTTCCCACTAAAAAGAGTTAGTTGCCTGATATCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTcc                                                                                                                     <  1:3510072/140‑1 (MQ=255)

                        ttcgCTTCCATTCTTTTTACAAGCGTTTTCTCCTCGCTTTGTCCTAAAATGCTCGTGAAACTTTTATCCTTTAGCACGAAAACGAGTTCGTTGCCTGATATCCCTTTTGTTTTCATAAACACGACTCGCCAACCGTCCgg                                                                                                                   <  2:658582/140‑1 (MQ=255)

                        ttcgCTTCCATTCGTTTTACAAGCGTTTTATCATCGCATTGTCCTAAAAAGCTCGTTAAAATTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                                   <  2:3496250/140‑1 (MQ=255)

                        ttcgCTACCATGCGTTTTACAAGCTTTTTCTAATCGCATTGTCCTAAAAAGGTCTTTAAACTTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAACACGACTCGCCAACCGTCCgg                                                                                                                   <  2:4695574/140‑1 (MQ=255)

                        ttcgATTCCATTCTTTTTACAAGCTTTTTCTAAGCGCTTTCTACAAAAAAGGTGGTTAAATTTTTATCTTTTCCCACCAAAAAGATTTAGTTGCCTGATATCCCTTTTTTATACATAAAAACGACTCGCCAACCGTCCgg                                                                                                                   <  1:4764059/140‑1 (MQ=255)

                        ttcgATTCAATTCTTTTTACATGCTTTTTATCTCGGCATTGTCCTAAAAAGCTCGTTAAACTTTTATCCTTTACCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                                                                                                                   <  2:1490139/140‑1 (MQ=255)

                        acagCTTCAATTCTTTTTAAAAGTTTTTTCTCATGGATTTGTCCTAAAAAGCTGGTTAAACTTTTACCCTTTACCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                                                                                                                   <  2:2166750/137‑1 (MQ=255)

                         tcgCTTACATTTTTTTAACAAGCTTTTCATACTCTCATTGTCATAAAACGCTCGTTAAACTTTTAACCTTCACCACAAAAAATATTTAGTGGCCTGATATCCCTTTTTTATTAAAAACCACGACTCGCCAACCGTCCGGa                                                                                                                  <  1:551960/140‑1 (MQ=255)

                          cgCTTCCTTTGGTTTTACAAGCGTTTACTCATCGCATTGTCCTAAAACGCTCGTTAAACTTTTTTCCTTTAGCACGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                                                                                                                 <  2:4915981/140‑1 (MQ=255)

                            cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                                               >  2:1768626/1‑140 (MQ=255)

                            cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                                               >  1:864238/1‑140 (MQ=255)

                                       tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCgctgt                                                                                                    >  1:2758919/1‑136 (MQ=255)

                                       tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCgctgt                                                                                                    >  1:2166750/1‑136 (MQ=255)

                                       tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                                    >  1:3220988/1‑140 (MQ=255)

                                       tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                                    >  1:2446960/1‑140 (MQ=255)

                                        ttACAATCTTTTTCTCATCGATTTTTCATAAAAAGCTCTTTAAACTTTTATCCTTTACCACGAAAAATTTTTACTTCCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                                                                                                   <  2:566033/140‑1 (MQ=255)

                                        ttACAAGCGTTTTCTCATCGCATTGTCAAAAAAAGATCGTTAAAATTTTATCATTTACCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCACTATCGTCACg                                                                                                   <  2:3708382/140‑1 (MQ=255)

                                             tgtgTTTTATCATCGCTTTTTCATAAAAAGCTCGTGAACATTTTTTCCTTTAGCACTAAAACTAGTTACTTGCCTTATATCCTTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTcc                                                                                              <  2:3393696/137‑1 (MQ=255)

                                                 ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                                                          >  2:1711000/1‑140 (MQ=255)

                                                  tttCTCATGGCATTGTCATAAAAAGCTCTTTAAACTTTTATCCTTTCGCACTAAAAAGATTTACTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                         <  2:89180/140‑1 (MQ=255)

                                                   ttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCt                                                                                        >  2:4347218/1‑140 (MQ=255)

                                                         tCGCTTTGTCCTAAAAAGCTGGTTAAACTTTTAGCCTTTACCACGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                                  <  2:3837518/140‑1 (MQ=255)

                                                         tCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                                  >  1:2907325/1‑140 (MQ=255)

                                                         tCGCATTGTCCTAAAAAGCTCGTGAAACTTGTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATACAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                                  >  1:1200812/1‑140 (MQ=255)

                                                         tCGCATTGTCCTAAAAAGCTCGTGAAAATTTTATACTTTAGCAAGAAAAAGAGTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTa                                                                                  >  1:89871/1‑140 (MQ=255)

                                                           gCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACg                                                                                >  2:1832783/1‑140 (MQ=255)

                                                                gTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtc                                                                           >  1:3773353/1‑140 (MQ=255)

                                                                 tcaaaaaacGCTCGTGAAACTTTTACCCTGTCGCACCAAAACGATTCACGGGCCTGATATCCTTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                                                          <  1:790280/136‑1 (MQ=255)

                                                                  ccTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtctc                                                                         >  2:3627166/1‑140 (MQ=255)

                                                                      aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                                                                     >  1:4373069/1‑140 (MQ=255)

                                                                      aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                                                                     >  1:2617024/1‑140 (MQ=255)

                                                                        ctgCTCGTTAATCTTTTCTCTTTTAGACCGAAATATATTTCGTGGCCTTATATCCGTTTGTTTTTCTAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  1:3124322/138‑1 (MQ=255)

                                                                        aaGCTGTTGAAACTTTTATCCTTGAGCACTAAAAAGAGTTACTTGCCTTATTTCCCTTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  2:1304252/140‑1 (MQ=255)

                                                                        aaGCTCGTTAAAGTTTTATCATTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  2:2445511/140‑1 (MQ=255)

                                                                        aaGCTCGTTAAAATTTTATCCTTTACCACGAAAAATATTTACTTGCCTTATATCCTTTTTGTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  1:46398/140‑1 (MQ=255)

                                                                        aaGCTCGTGAAACTTTTATCATTTAGCACTAAAAAGAGTTAGTTGCCTGATTTCCCTTTTGTATAAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  2:3931621/140‑1 (MQ=255)

                                                                        aaGCTCGTCAAACTTTTATCCTTGAGCACGAAAATGATTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                   <  2:761308/140‑1 (MQ=255)

                                                                          gCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCaa                                                                 <  2:2450268/140‑1 (MQ=255)

                                                                           cttgTGAAAATTTTATCCTTTACCACCAAAAAGTTTTCTTTGCCTGTTATCCTTTTTGTTTTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                <  1:1452407/137‑1 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:21452/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:4037451/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:3234473/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:1513062/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:103966/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:100527/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:4545830/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:3286166/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:2117972/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  1:639205/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:1039972/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:1140247/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACAAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                                >  2:3792547/1‑140 (MQ=255)

                                                                            tctttaaaTTTTTATCCTTCCCCAGTAAAATGTGTTACTTGCCTTATAGCCTTTTTTTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                                                               <  1:2357003/135‑1 (MQ=255)

                                                                            tCGTTAAAATTTTATCCTTCCCCACCAAAAATTGTTTCTTGCATTATATCCCTTTTGTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTATGGGTTCTCAGATTCAAcc                                                               <  1:1609718/140‑1 (MQ=255)

                                                                            tCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAcc                                                               <  2:2281192/140‑1 (MQ=255)

                                                                            ccctcaaaCTTTCACCCTTTCCCACCAACACGCGTTCCTTCCCTCATAGCCCTTGTCTCTCCAAAAACACGACTCGCCAACCGTCCGGCTCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAccc                                                               <  2:1642626/135‑1 (MQ=255)

                                                                                  aatttttAGCCTTTACCACTAAAAAGAGTTACTTGCCTTATATCCCTTTTTTATTAATAACCACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                                         <  1:419879/137‑1 (MQ=255)

                                                                                  aaCTTTTATCCTTTACCACGAAAAAGAGTTACTTGCCTGATATCCATTGTGTATTCATAACAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTGCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                                         <  2:4723951/140‑1 (MQ=255)

                                                                                  aaCTTTTACCCTTTAGCAATAAAAATATTTACTTGCCTTATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGa                                                         <  1:3593027/140‑1 (MQ=255)

                                                                                         aTCCTTTCGCACTAAAAATATTTAGTTGCCTTATATCCATTTTTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                                  <  1:222843/140‑1 (MQ=255)

                                                                                         aTCCTTGAGCACTAAAAAGTTTTCCTTGCCTGATACCCATTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                                  <  1:201116/140‑1 (MQ=255)

                                                                                          tCCTTTAGCACTAAAAAGTGTTACTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                                 <  2:4151162/140‑1 (MQ=255)

                                                                                          tCCTTTAGCACGAAAATGAGTTAATTGCCTGATATCCCTTTTTTCTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                                 <  2:4466557/140‑1 (MQ=255)

                                                                                          tCCTTTAGCACCAAACAGTGTTACTGGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                                 <  2:4415544/140‑1 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  1:3343782/1‑140 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  2:461635/1‑140 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  1:4671648/1‑140 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAGCCTCTATCGTCACGCCTCCCTGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                            >  2:4793403/1‑140 (MQ=255)

                                                                                                cgCACTAAAAAGAGTTACTGGCCTTATAGCCCTTTTGTTTTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  1:153338/139‑1 (MQ=255)

                                                                                                accccGAAAAAGAGTTACTTGCCTTATCTCCCTTTTTTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                           <  2:2005159/136‑1 (MQ=255)

                                                                                                             gTTACTTGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTg                              <  2:2389932/140‑1 (MQ=255)

                                                                                                               tagttGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGAt                            <  2:4576521/137‑1 (MQ=255)

                                                                                                               tACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGAt                            >  1:2198534/1‑140 (MQ=255)

                                                                                                                 ctggCCTGATACCCCTTTTGTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCg                          <  1:190450/137‑1 (MQ=255)

                                                                                                                      cTGTTATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGACCGGATCCTCTAACGACACGCCTCCATGGCTCCAGTACGGGGTCTCAGATTCAACCTCTTGATAGCCCATGTGTATTTGCTTTGAAGTGATCGCTGTc                     >  2:1921738/1‑140 (MQ=255)

                                                                                                                      cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTc                     >  2:837969/1‑140 (MQ=255)

                                                                                                                      cTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGACACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGATTTAGCTTTGAAGTGATCGCTGTc                     >  2:3089224/1‑140 (MQ=255)

                                                                                                                        gATATCCTTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAg                   <  2:1593600/140‑1 (MQ=255)

                                                                                                                        gATATCCCATTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCGCCATGGCTCCAGTCCGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAg                   <  2:2067398/140‑1 (MQ=255)

                                                                                                                        gATAACCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAg                   <  2:2653517/140‑1 (MQ=255)

                                                                                                                          tatCCATTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGtt                 <  2:1200812/140‑1 (MQ=255)

                                                                                                                              ccTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGt              >  2:2662419/1‑139 (MQ=255)

                                                                                                                               cTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt            <  2:707794/140‑1 (MQ=255)

                                                                                                                               cTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt            >  1:4973456/1‑140 (MQ=255)

                                                                                                                               cTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt            <  2:2759208/140‑1 (MQ=255)

                                                                                                                               cTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGACTCCATGGCTCCAGTACCGGTTCTCAGATTCAACCTCTTCATAGCCCATGTGATTTAGCTTTGAAGTGATCGCTGTCAGTGTGTCt            >  2:3988679/1‑140 (MQ=255)

                                                                                                                                 ttagTATTCATAAAAACGACTCGCAAACCGTCAGCATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGc          >  1:1333116/4‑140 (MQ=255)

                                                                                                                                 ttagTATTCATAAAAACGACTCGAAAACAGTCCGCATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGc          >  1:99866/4‑140 (MQ=255)

                                                                                                                                   tGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCAt        >  2:1863374/1‑140 (MQ=255)

                                                                                                                                       ttCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg    >  1:249702/1‑140 (MQ=255)

                                                                                                                                       ttCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg    <  1:3459952/140‑1 (MQ=255)

                                                                                                                                        tCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGc   >  2:4185421/1‑140 (MQ=255)

                                                                                                                                         cATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGca  >  1:411095/1‑140 (MQ=255)

TAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGCA  >  NZ_CP034943/3996790‑3997066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`