Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 4,022,597 G→T 5.5% V607F (GTT→TTT)  EO946_RS20675 → thioredoxin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349434,022,5970GT 5.5% 940.7 / 5.0 368V607F (GTT→TTT) EO946_RS20675thioredoxin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (153/175);  new base T (4/34);  total (157/211)
Fisher's exact test for biased strand distribution p-value = 8.29e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 377 total aligned reads displayed.

AGTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTA  >  NZ_CP034943/4022458‑4022736
                                                                                                                                           |                                                                                                                                           
aGTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCg                                                                                                                                             <  2:435539/140‑1 (MQ=255)
          gACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTgg                                                                                                                                   <  1:3038472/140‑1 (MQ=255)
           acgtATCACTCATTGAAAAAGCAGAAACGATGGTTTCGGTGTTCAAGCCGGATATCGAAGCGTCTCCGAGCGGCCATGCTTTCTTTATGCAAAGGGTGGTAAAGCATGTGATGCCTACAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  2:4280145/4‑140 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAATGAAATCGTTATTTTTGGc                                                                                                                                  >  1:3585366/1‑140 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  1:873000/1‑140 (MQ=255)
               aTCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAgcg                                                                                                                              >  1:4950411/1‑140 (MQ=255)
               aTCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAgcg                                                                                                                              >  1:200325/1‑140 (MQ=255)
                    tgatcgAAAAAGCGGAAACAATGTTTTCCGTGTTCAAGCCAGACATAGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGTGTGTTAAGGCATCTGATGCCTAAAAAAGAAATCGTGATTTTTg                                                                                                                                    >  2:3999234/6‑129 (MQ=255)
                      aTTGACAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCACAGCGTGTTAAAGCATGTGATGCCTATAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:2749039/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:1028472/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:2819961/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:4325555/1‑140 (MQ=255)
                      aTTGAAAAAGAAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  1:4384418/1‑140 (MQ=255)
                        tGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATc                                                                                                                     >  1:2859085/1‑140 (MQ=255)
                            aaaGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATccagc                                                                                                                 >  2:3166366/1‑140 (MQ=255)
                              aGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATccagcca                                                                                                               >  1:1308303/1‑140 (MQ=255)
                              aGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATccagcca                                                                                                               >  2:3723969/1‑140 (MQ=255)
                                cAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGa                                                                                                             >  1:1632176/1‑140 (MQ=255)
                                  gAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaa                                                                                                           >  2:4447984/1‑140 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  2:451674/140‑1 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  1:4120742/140‑1 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  1:2762593/140‑1 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  1:2907426/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:2297055/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:3166366/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:4227608/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:4321738/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:1311850/140‑1 (MQ=255)
                                        aTGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:1027504/140‑1 (MQ=255)
                                                   gtTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCAc                                                                                          <  1:3867006/140‑1 (MQ=255)
                                                   gtTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCAc                                                                                          <  2:1850501/140‑1 (MQ=255)
                                                          ccGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCaaaa                                                                                   <  2:1651825/140‑1 (MQ=255)
                                                           cGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCaaaaa                                                                                  <  2:4172645/140‑1 (MQ=255)
                                                              atatCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGcc                                                                               >  2:349516/1‑140 (MQ=255)
                                                              atatCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGcc                                                                               >  2:3284529/1‑140 (MQ=255)
                                                                atCTAAGCGTATCCGAGCGGCAATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATTCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAGAAATCACCACTGCACTTCAAAAAGCCtt                                                                             <  2:4585309/140‑1 (MQ=255)
                                                                atCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCtt                                                                             <  2:2269228/140‑1 (MQ=255)
                                                                    aaGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAg                                                                         <  2:541217/140‑1 (MQ=255)
                                                                       cGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGccc                                                                      >  2:292605/1‑140 (MQ=255)
                                                                           tCCGAGCGGCCATGCTTTCTTTATGCACAGCGTGTTAAAGCATGTGATGCCTAAAAAGCAAATCGTTATTTTTGGCAGCGCCGTCGATCCAGCCCGCAAACACGTCACCACTGCACTGCAAAAAGCCTTTAAGCCCCACg                                                                  >  1:2658008/1‑140 (MQ=255)
                                                                               aGCGGCCATGCTTTCTTTATGCAAAGCTTGTTTAAGAATGTCATGCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCAGACGATCCAGCAAGAAAACAAATCACAACTGCACTTAAAAAAGCATTTAAGCCCAACGACTc                                                              <  2:841446/140‑1 (MQ=255)
                                                                               aGCGGCCATGCTTTATTTATGCAAAGCGTGTTAAAGCATGTTATGCCTAAAAATTAAATCTTTATTTTTGGCAGCGCAGAGGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTc                                                              <  2:1940882/140‑1 (MQ=255)
                                                                                 cGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACACATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCa                                                            >  1:1272284/1‑140 (MQ=255)
                                                                                 cGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCa                                                            >  1:2575285/1‑140 (MQ=255)
                                                                                    ccATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAttt                                                         >  1:3373218/1‑140 (MQ=255)
                                                                                     cATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCAtttt                                                        >  2:3104422/1‑140 (MQ=255)
                                                                                              tttcggCAAAGCGTTTTAACGATTGTTCTGCATAACACGCAATCCGTTATTTTTGGCAGCCCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAACCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  1:752485/135‑1 (MQ=255)
                                                                                              tttATGCAATGCGTGTTAAAGCATGTGATGCATAAAAAGGAAAGCTTTATTTTTGGCAGCGCAGACTATCCAGCCAGAAAACAAATCACCACGGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  2:2223746/140‑1 (MQ=255)
                                                                                              tttATGCAAAGGGTGTTAACGCATGTGATGCCTAAAAAGGAAAGCGTTATTTTGGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTAAAAACGCCTTTAACCCCAACGACTCCATTTTGGTGGCaga                                               <  2:486105/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCTTTTTAAACCATGTGATGCCTAAAAAGCAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  2:4837165/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTTTTAACGCATGTGATGCCTAAAAAGGAAATCGTTATTTTGGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  2:4694157/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTTTTAAAGCATGTGAGGCCTAAAAAGCAAATCTTTATTTTGGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTCCACTACAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  1:953429/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  1:1931903/1‑140 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTCCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  2:1704041/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACAACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  2:2033307/1‑140 (MQ=255)
                                                                                              tttATCCAACGCGTGTTAACGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCACAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCCACGACTCCATTTTGGTGGCaga                                               <  2:1303674/140‑1 (MQ=255)
                                                                                              tttAGGCAACGCGTGTTACCGCATGTGAGGCCTAAAAAGGAACTCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               <  2:1422467/140‑1 (MQ=255)
                                                                                               ttATGCAAACCGTGTTAAAGCAGTTGATGCCTAAAAAGCAAAGCGTTATTTTTGGCAGCGCAGACGATCCAGCAAGAAAAAAAAACACCACTGAACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCagag                                              <  1:4850246/140‑1 (MQ=255)
                                                                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCAt                                           >  2:2539195/1‑140 (MQ=255)
                                                                                                   gCAAAGCGTGTTACAGCATGTGATGCCTAAAAAGCAAATCGTTATTTTTGGCAGCGCAGACGAGCCACCCAGAAAACAAATCACCACTGCACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATc                                          <  2:3799824/140‑1 (MQ=255)
                                                                                                   gCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATc                                          >  2:3309412/1‑140 (MQ=255)
                                                                                                      aaGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTg                                       >  2:1474756/1‑140 (MQ=255)
                                                                                                      aaGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTg                                       >  1:3991285/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  2:2103619/1‑140 (MQ=255)
                                                                                                        gCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGAt                                     <  2:421773/140‑1 (MQ=255)
                                                                                                        gCGTGTTAAAGCATGTGATGCCTAAAAAGCAAATCGTTATTTTTGGAAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGAt                                     <  2:1145609/140‑1 (MQ=255)
                                                                                                         ggtgtTAAAGCATGTTATGCATAAAAAGCAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCATAAAAAAAATCACCACTCAACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                    <  1:144067/139‑1 (MQ=255)
                                                                                                         cGTTTTAAAGCTTTTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCCGCCAGAAAAAAAATCACCAGTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                    <  1:3790005/140‑1 (MQ=255)
                                                                                                         cGTGTTAAAGCATGTGATCCCTAAAACCCAAAACGTTATTTTGGGCAGCGCAGACGATCCCGCCAGAAAACAAATCACCACTGCACTACAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                    <  1:3459331/140‑1 (MQ=255)
                                                                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAAt                                 >  1:4712381/1‑140 (MQ=255)
                                                                                                            gtTAAAGCATGTGATCCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCAGACGATACAGCCAGAAAAAAAATCACCACTGCACATCAAAAAGCCTTTAAGCCAAACGACTCAATTATGGTGGCAGAGCAACCTGATCAAt                                 >  1:2542597/1‑140 (MQ=255)
                                                                                                                acgcttgtgAGGCCAAAAAAGCAAATGGTTTTTGTTGGCCGGGCAGACGACCCACCCACAAAAAAAATCACCACGGCACTTCAAACAGACTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                             <  1:1861068/135‑1 (MQ=255)
                                                                                                                aacaaTGTGATGCAAAAAAAGGAAATCGTTATTTTTGGCAGCGAAGACTATCCAGCAAGAAAAAAAATCACCACTGCACTTCAAAACGCATTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                             <  1:42984/136‑1 (MQ=255)
                                                                                                                aaGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCAAGAAAACAAATCACCACTGCACTTCAAAAACCGTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                             <  2:368499/140‑1 (MQ=255)
                                                                                                                 aGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaaa                            >  1:2365974/1‑140 (MQ=255)
                                                                                                                  gCATGTGATGCATAAAACGTAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTAAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                           <  1:1200360/140‑1 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATACTGATCAATGCAAAGACa                        >  1:1789350/1‑140 (MQ=255)
                                                                                                                     aattattccTAAAAAGGAAATCTTTATATTAGTCACCGCATAAAAAAAATCAAGAAAAAAAATCAACACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATATTGTTGGCAGAGCATCATGATAAATGCAAATACa                        >  1:1092290/8‑140 (MQ=255)
                                                                                                                      gtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACAt                       >  2:736556/1‑140 (MQ=255)
                                                                                                                      gtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACAt                       >  2:4917940/1‑140 (MQ=255)
                                                                                                                       tgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATa                      >  2:1447508/1‑140 (MQ=255)
                                                                                                                       tgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATa                      >  2:1249353/1‑140 (MQ=255)
                                                                                                                         ttGCCTAAAACGGAACTGTTTATTTTTGCCAGCGCAGACCACCCACCCACAAAAAAAATCACCACGCCACTTCAAAACGCATTTACCCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGc                    <  1:1703910/139‑1 (MQ=255)
                                                                                                                          tcccTAAAAATTAAATCTTTATTTTTGGCAGCGCAGACGATCCACCAATAAAAAAAAACACCACGGCACTTCAAAAAGCCTTTAAGCACAACGACTCCATTTTGTTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                   <  1:1501748/138‑1 (MQ=255)
                                                                                                                          tGCATAAAAAGCAATTCTTTATTTTTGTCAGCTAAGACTATCCAGCCAGAAAAAAAATCACAACTTAACTTAAAAAAGCATTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                   <  1:4589141/140‑1 (MQ=255)
                                                                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCCCCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 >  2:3942953/1‑140 (MQ=255)
                                                                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 >  2:3417713/1‑140 (MQ=255)
                                                                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 <  1:3663110/140‑1 (MQ=255)
                                                                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTGTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 >  2:2489283/1‑140 (MQ=255)
                                                                                                                            catAAAAATGAAATCTTTATTTTGGGCAGCGCATACGCTCCAGCCAGAAAACAAAAAACCACTCCAGTTCAAAACGCCTTAAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 <  1:572250/138‑1 (MQ=255)
                                                                                                                             cTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAccc                >  2:3047832/1‑140 (MQ=255)
                                                                                                                             atAAAAAGCAAATCCTTAGTTTGGCCAGCGCACACGACCCCTCCATAAAAAAAACCACCACTGCAAGACAAAAAGCATGTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAccc                <  1:329971/139‑1 (MQ=255)
                                                                                                                              tAAAAAGCAATACGTTTTTTATGCATGCGAAGACCTTCAAGCCAGAAAAAAAAACAACAATGAAATAAAATCACCAATTACCCCCAACGACTCCATTTTGGTTGCAGAGCATCATGATCAATGCTAAGACATAGCACCCt               <  1:2101755/140‑1 (MQ=255)
                                                                                                                                 aaaGTAACTCGTTATTTTTGGCAGCGCAGACGATCCAGCCATAAACAAAATCACCACTGCACTTCAAAAAGCCTTTACGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTg            <  2:3211043/140‑1 (MQ=255)
                                                                                                                                 aaaGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAACCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTg            <  2:1550994/140‑1 (MQ=255)
                                                                                                                                   cggATATCGTTATTTTGGGCAGCGCATACGATCCAGCCATAAAACAAATCACCACTGCACTACAAAAAGCCTTAAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAATGACATAGCACCCTTTgca          <  2:239469/139‑1 (MQ=255)
                                                                                                                                      aaaTCTTTATTTTTGGCAGCGCAGACTATCCAGCCATAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcagca       <  2:3854206/140‑1 (MQ=255)
                                                                                                                                      aaaTCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcagca       >  2:4723602/1‑140 (MQ=255)
                                                                                                                                      aaaTCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcagca       >  2:3639442/1‑140 (MQ=255)
                                                                                                                                        aTCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCa                                                                                           <  2:2620021/54‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTA  >  NZ_CP034943/4022458‑4022736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: