BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	443,396	G→T	6.4%	G261V (GGA→GTA)	gdh →	glucose 1‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	443,396	0	G	T	6.4%	782.2 / 4.6	300	G261V (GGA→GTA)	gdh	glucose 1‑dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (178/87); new base T (2/27); total (181/119)
Fisher's exact test for biased strand distribution p-value = 1.78e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

ATGGGATATATCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGAAGATCC  >  NZ_CP034943/443257‑443535

aTGGGATATATCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTCGGAGCCAATATCCTTCCTTCCAGGCAGGACGCgg                                                                                                                                             >  1:2704256/1‑140 (MQ=255)

aTGGGATATATCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCgg                                                                                                                                             >  1:3563743/1‑140 (MQ=255)

aTGGGATATATCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCgg                                                                                                                                             >  1:1406870/1‑140 (MQ=255)

        tatCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATaaaaaa                                                                                                                                     >  2:3586961/1‑138 (MQ=255)

        tatCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACa                                                                                                                                     >  2:50486/1‑140 (MQ=255)

         atCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaa                                                                                                                                    >  2:4624287/1‑140 (MQ=255)

             gTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaa                                                                                                                                >  2:817618/1‑140 (MQ=255)

             gTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaa                                                                                                                                >  2:2846664/1‑140 (MQ=255)

             gTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaa                                                                                                                                >  1:2345004/1‑140 (MQ=255)

              tGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaaa                                                                                                                               >  1:2104374/1‑140 (MQ=255)

              tGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaaa                                                                                                                               >  1:686229/1‑140 (MQ=255)

              tGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACaaaaaaa                                                                                                                               >  1:182231/1‑140 (MQ=255)

                                     tAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATACAACAAAAAATGCGACCCCGAACTCATCTGGATc                                                                                                        >  2:2757354/1‑140 (MQ=255)

                                     tAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATc                                                                                                        >  1:3985254/1‑140 (MQ=255)

                                     tAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATc                                                                                                        >  1:164111/1‑140 (MQ=255)

                                     tAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATc                                                                                                        >  1:636607/1‑140 (MQ=255)

                                        cagcCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCt                                                                                                     <  2:636607/140‑1 (MQ=255)

                                          gcCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCttt                                                                                                   <  2:3408646/140‑1 (MQ=255)

                                            cTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCCGAACTCATCTGGATCGCttttg                                                                                                 >  2:4609606/1‑139 (MQ=255)

                                               gcttgcttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCtt                                                                                              >  1:3072718/1‑140 (MQ=255)

                                               gcttgcttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTGTTTCtt                                                                                              >  2:2187788/1‑140 (MQ=255)

                                                   gcttGGAAGGAAGCCAGCGACGTCCCAGGCACCCCGTTATTCGGGGACGGCGGTTTGACCCAAGATCCTTCCTGCCAGGCAGGACGCGGATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAgg                                                                                          <  2:830268/140‑1 (MQ=255)

                                                   gcttCGATGTAAGCCAGCTCCGTCACAGGCTTAACGTTATTCGCGGACGGCGTTATGACCCAATATCCTTCCTGCCAGGCAGGAAGCGCATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAgg                                                                                          <  2:484422/140‑1 (MQ=255)

                                                   gcttCGAAGTAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCAAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAgg                                                                                          <  2:3735307/140‑1 (MQ=255)

                                                    cttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGgc                                                                                         >  2:2313100/1‑140 (MQ=255)

                                                    cttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGgc                                                                                         >  2:3536854/1‑140 (MQ=255)

                                                    cttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGgc                                                                                         >  2:2139700/1‑140 (MQ=255)

                                                     ttCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGgcg                                                                                        >  2:672201/1‑140 (MQ=255)

                                                     taccaaggaagCCAGCTACGTCACAGGCATCACGTGATTCGCGGACGGCGGTAGGACCCAAAATCCTACCTTCCACCCACGACGCGGATCAAACAAAAAAACCGACCCACAACTCATCTGGATCGCTTTTTCTTTCGgcg                                                                                        >  2:2658389/5‑140 (MQ=255)

                                                           tgacgCCATCTACTTCACAGTAATCTCTTTATTGGCGGCGGGCGTTTTCACCCAATATCATTCATCCCCGGCAGGACGCTCATAAAAAAAAAAAAGCGACCCAGAACTCCTCTGGATCGCTTTTTCTTTAGGCGCGCttt                                                                                  <  1:1579734/136‑1 (MQ=255)

                                                           tgaagCCAGCTACGTCACAGGAATCACGTTATTCGCGGACGGCGTTTTGACCCAATATCCTGCCTTCCAGGCAGGACGCGGATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCttt                                                                                  <  2:1264018/139‑1 (MQ=255)

                                                           cgacccaaGCAACGTCACCGGCATCACGTTATTCGCGCAGGGCGTTATGACCAACTATCCGGCGGACCCGGCCGGACGGGTTTAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCttt                                                                                  <  1:4716323/133‑1 (MQ=255)

                                                              agCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTc                                                                               >  1:417320/1‑140 (MQ=255)

                                                              agCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTc                                                                               >  1:4628613/1‑140 (MQ=255)

                                                               gCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGTACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTGTTCGTTAGGCGCGCTTTTTCt                                                                              >  1:1222052/1‑140 (MQ=255)

                                                                    cTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCCGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACa                                                                         <  2:734964/140‑1 (MQ=255)

                                                                    cTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACa                                                                         <  2:4412197/140‑1 (MQ=255)

                                                                      aCGTCACCGGCATCACGTTATGGGCGGACGGCGGTATCACCCAATAGCCTTCCTTCCAGCCAGCACGCGAATAAAAAAAAAACAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAag                                                                       <  1:4002932/140‑1 (MQ=255)

                                                                         tCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGc                                                                    >  2:1847927/1‑140 (MQ=255)

                                                                         tCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTGTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGc                                                                    >  2:4255778/1‑140 (MQ=255)

                                                                                  tCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGa                                                           >  1:3614632/1‑140 (MQ=255)

                                                                                   cACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGAt                                                          >  2:3930119/1‑140 (MQ=255)

                                                                                   cACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGAt                                                          >  1:4446638/1‑140 (MQ=255)

                                                                                   cACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGAt                                                          >  1:219434/1‑140 (MQ=255)

                                                                                   cACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGAt                                                          >  1:3428284/1‑140 (MQ=255)

                                                                                         aTTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaata                                                    >  1:1006180/1‑140 (MQ=255)

                                                                                         aTTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaata                                                    >  1:1784370/1‑140 (MQ=255)

                                                                                         aTTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaata                                                    >  2:1982725/1‑140 (MQ=255)

                                                                                         aTTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaata                                                    >  2:2541619/1‑140 (MQ=255)

                                                                                         aTTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaata                                                    >  1:4083863/1‑140 (MQ=255)

                                                                                           tCGCGGACGGCGTTATGAGCCAATATCCTTCCTTCCTGGACGGACGCTTATAAAACAAAAAAAGCGACCCAGAACTCAACTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaataat                                                  <  2:2059707/140‑1 (MQ=255)

                                                                                           tCGCGGACGGCGGTATGACCCACTATCCTTCCTTCCCGGCAGTACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAaataat                                                  <  2:1456886/140‑1 (MQ=255)

                                                                                                gACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTg                                             >  2:3841592/1‑140 (MQ=255)

                                                                                                  cggcggTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGcc                                           <  2:1198402/140‑1 (MQ=255)

                                                                                                   ggcggTATGACCCAATATCCTTCCTTCCATGCAGGACGCGGATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCg                                          <  2:4051559/140‑1 (MQ=255)

                                                                                                   ggcggTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCg                                          >  1:1881137/1‑140 (MQ=255)

                                                                                                       gTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGTATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGa                                      <  2:2376034/140‑1 (MQ=255)

                                                                                                        taagACCCAATATCCTTCCTTCCAGGCAGGACGCGTATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGAt                                     <  2:2796665/137‑1 (MQ=255)

                                                                                                        tATGACCCAATATCCTTCCTTCCCGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGAt                                     <  2:581588/140‑1 (MQ=255)

                                                                                                        tATGACCAAATCTCCTTCCTTCCAGGCAGGACGCGTATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGAt                                     <  1:2430678/140‑1 (MQ=255)

                                                                                                                aagagccttcctcCCAGGCAGGACGCGTATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGAt                             <  1:4560299/135‑1 (MQ=255)

                                                                                                                aaTATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGAt                             <  1:105412/140‑1 (MQ=255)

                                                                                                                  tatCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCg                           <  1:1433443/140‑1 (MQ=255)

                                                                                                                  tatCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCg                           <  2:4946035/140‑1 (MQ=255)

                                                                                                                  tatCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCg                           <  1:895881/140‑1 (MQ=255)

                                                                                                                  tatCCGTCCGTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCg                           <  2:180900/140‑1 (MQ=255)

                                                                                                                  aagcctccctcCCCGCCAGCACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCg                           <  1:3506577/137‑1 (MQ=255)

                                                                                                                    tccttcctTCCAGGCAGGACGCGGATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgct                         <  2:3125159/140‑1 (MQ=255)

                                                                                                                       ttccttccCGGCAGGCCGCGGATAAAACACAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTGACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  1:1372300/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGTACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  2:88717/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGGCCGCGGATAAAAAAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  1:3690814/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCTGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  1:3251277/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGTATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  1:1713328/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGTATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  2:3283547/140‑1 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGCTAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      >  1:4507665/1‑140 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      >  1:2544535/1‑140 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      >  1:2606357/1‑140 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      >  2:1816237/1‑140 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      >  1:4862025/1‑140 (MQ=255)

                                                                                                                       ttccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTGTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgcg                      >  2:802164/1‑139 (MQ=255)

                                                                                                                       ttcctcccCGGCAGGACGCGGATAAAACAAAAAACGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctgct                      <  1:1977565/140‑1 (MQ=255)

                                                                                                                        tccttccAGGCAGGACGCGGATAAAACAAATAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTACTTTCCAAGAGCTCCAACAGATAAAATACTGGCTGCCGCAGAGAGCACGATCGCTCCta                     >  2:3468103/1‑140 (MQ=255)

                                                                                                                        tccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGTTAGCACGATCTCTGCta                     >  2:3952188/1‑140 (MQ=255)

                                                                                                                        tccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCta                     >  1:3878001/1‑140 (MQ=255)

                                                                                                                        tccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCta                     >  1:1104691/1‑140 (MQ=255)

                                                                                                                        tccttccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCta                     >  1:311060/1‑140 (MQ=255)

                                                                                                                            tccAGGCCGGACGCGGATAAAACAAAAAACGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATc                 <  1:2881959/140‑1 (MQ=255)

                                                                                                                            tccAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATc                 >  2:496331/1‑140 (MQ=255)

                                                                                                                                ggcaggACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATgg                                                >  1:3834867/1‑105 (MQ=255)

                                                                                                                                ggcaggACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCgctg                        >  2:1579953/1‑129 (MQ=255)

                                                                                                                                    ggAGGCGTATAAAACAAAAAATGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCAGCTATATCAAGAGCTg         <  2:3675263/140‑1 (MQ=255)

                                                                                                                                    ggACGCTTATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTg         <  1:4321649/140‑1 (MQ=255)

                                                                                                                                     gACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGcc                                           <  2:2917812/105‑1 (MQ=255)

                                                                                                                                     gACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGcc                                           >  1:2917812/1‑105 (MQ=255)

                                                                                                                                     gACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGa        >  2:1631349/1‑140 (MQ=255)

                                                                                                                                     gACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGa        >  2:4904049/1‑140 (MQ=255)

                                                                                                                                     gACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGa        >  2:4431847/1‑140 (MQ=255)

                                                                                                                                      aCGCGTATAAAAAAAAACAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGGGCTTTTTCTTTACAAGAGCTTCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGaa       <  2:4496859/140‑1 (MQ=255)

                                                                                                                                         cgGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGAAGAt    >  2:1674580/1‑140 (MQ=255)

                                                                                                                                           gATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGa                                      <  2:1097137/104‑1 (MQ=255)

ATGGGATATATCGGTGAACCGGAGGAAATTGCGGCAGTAGCAGCCTGGCTTGCTTCGAAGGAAGCCAGCTACGTCACAGGCATCACGTTATTCGCGGACGGCGGTATGACCCAATATCCTTCCTTCCAGGCAGGACGCGGATAAAACAAAAAAAGCGACCCAGAACTCATCTGGATCGCTTTTTCTTTAGGCGCGCTTTTTCTTTACAAGAGCTGCAACAGATAAAATAATGGCTGCCGCAGATAGCACGATCGCTGCTATATCAAGAGCTGAAGATCC  >  NZ_CP034943/443257‑443535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`