BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,529	G→C	5.2%	A124A (GCG→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,529	0	G	C	5.2%	407.8 / 5.1	152	A124A (GCG→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (61/78); new base C (4/6); total (67/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

GAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607392‑607665

gacgccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                                        <  1:795939/137‑1 (MQ=255)

gAAGCCGGACCCGCCGGACCACCCGTAGCTACAGGAGCGACAGGTGATACCGGGGAAGCCGGACCAGCGGGAGCTACTGGAGCGACAGTAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                                        <  1:1513700/140‑1 (MQ=14)

gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                                        <  1:2328370/140‑1 (MQ=255)

   gccggaccagccggaccaACCGGAGCGACAGGAGCGATAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                                     >  2:308129/1‑140 (MQ=255)

     cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                                   >  1:644924/1‑140 (MQ=255)

       gaccagccggccccACCGGCGCGCCGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGACCGACCGGAGCGACAGGAGCGACAGGATATACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCt                                                                                                                                 <  2:544218/140‑1 (MQ=11)

       gaccagccggacccACCGGAGCGACCGGAGCTACTGGAGCTAGCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGTGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCt                                                                                                                                 <  2:2168062/140‑1 (MQ=14)

       ccccccccgcaccaCCCGGAGCGCCCGGCGCGACTGGAGCTACCGGCGAACCCGGTCCAGCCGCAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGCGGAAGCTGGACCCGCGGGAGCGACGGGAGCGACAGGAGCt                                                                                                                                 <  2:2298337/134‑1 (MQ=14)

            gccggaccaACCGGAGCGACAGGAGCGATAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                                            <  1:308129/140‑1 (MQ=255)

            gccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                                            >  2:375673/1‑140 (MQ=255)

             ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGGGCTACCGGCGCTACCGTGGACGGGGGACAAGCCGGGGCGAGGGGAGCGAGCGGGGCTGCCggg                                                                                                                           >  1:1428625/1‑140 (MQ=14)

             ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCACCCGGAGCAGCTGGAGCAGCTGGCGCGACGGGAGCGCCAGGAGCGACCggg                                                                                                                           >  1:2500814/1‑140 (MQ=17)

             ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCCGGAGCCGCCGGAGCCACAGGCGCGACAGGAGCGACCGGAGCAACCGGGGCAGCGGGACAAGAGGGAGCTACCGGAGATAAAGGAGCAACCggt                                                                                                                           >  1:1265927/1‑139 (MQ=9)

                  cctccgggagcgacaggagccacaGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                      <  1:2710753/134‑1 (MQ=255)

                      ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGTAGCTACCGGGGAAGCTGGACCAGCTGCAGCGACGGGAGAGACAGGAGATAACGGGGAAGCCGGa                                                                                                                  >  2:1596664/1‑140 (MQ=18)

                      ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGa                                                                                                                  >  2:663643/1‑140 (MQ=255)

                      ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTCCCGGAGAAGCGGGACCAGCAGGAGCGACTGGAGCCACAGGAGCTACCGGAGAAGCCGGa                                                                                                                  >  1:1419871/1‑140 (MQ=17)

                       cggagcgaccggagcgacAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                 <  2:1960720/140‑1 (MQ=18)

                           gcgacccgaccgccaggaCCTACCGGCGCAGCCGGACCAGCCGGACCGCCAGGAGCGACAGGAGCCACAGGAGCAACCGGCGCAGCCGGACCAGCTGGAGCGACGGGAGCCACAGGAGCGACCGGGGAAGCCGGACCAGc                                                                                                             <  2:2129233/140‑1 (MQ=14)

                            cgacaggagcgacaggagCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                                            <  1:1947388/140‑1 (MQ=255)

                                aggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                                           <  2:1651716/121‑1 (MQ=255)

                                  gagcgacaggagcGACCGGGGACGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCACCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGcc                                                                                                      <  1:2399790/140‑1 (MQ=17)

                                   agcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                                     <  1:2366032/108‑1 (MQ=255)

                                   agcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                                     >  2:2366032/1‑108 (MQ=255)

                                    gcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                    >  1:315398/1‑140 (MQ=255)

                                     cgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                                   <  1:1086674/140‑1 (MQ=255)

                                      caccggagcGACCGGTGAACCCGGACCAGCCGGACCCCCCGGCGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACgg                                                                                                  <  1:2842712/136‑1 (MQ=14)

                                       tctggagcTACGGGGGAAGCCGGACCACCCGGCCCTCCAGGTGGGACTGGAGCCACTGGTGGTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:2422823/137‑1 (MQ=14)

                                       ccaggcgcTACCGCGGAAGCCCGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:2637538/139‑1 (MQ=255)

                                       actggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:1571771/137‑1 (MQ=255)

                                       acaggagcTCCCGGCGCAGCCGGACCACCCGGAGCCACAGGAGCGACAGGACCGACAGGACCTACCGGGGAAGCTGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:1074745/140‑1 (MQ=18)

                                       acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:148488/140‑1 (MQ=255)

                                       acaggagcTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:3020864/140‑1 (MQ=255)

                                       acaggagcTACCGGCGCAGCCGGCCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGCCCTGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                                 <  1:724692/140‑1 (MQ=18)

                                         cgccgcgaCCGGGGAAGCCGGCCCAGCCGCAGCGACACGAGCGACAGGACCTACAGGCCCTACCGGGGTCGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACAGGAg                                                                                               <  2:1652082/133‑1 (MQ=17)

                                           gctgtACGGGGGACGCCGGACCTCCCGGAGCGCCAGGAGCCCCAGGAGCGCCAGGTCCCACCGGCGAAGCTGGACCAGCTGGAGTGACGGGAGCGACAGGAGCTCCGGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                                                             <  2:1550990/136‑1 (MQ=18)

                                             gcgaCCGGGGAAGCCGGCCCAGCCGGCGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGTAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                           <  1:2974477/137‑1 (MQ=255)

                                             gcTCCCGGGGAAGCCGCTCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                           <  1:187537/140‑1 (MQ=255)

                                             ccgaCCGGGGAAGCCGGCCCCCCCGGCGCTACAGGCCCCACCCGAGCGACCGGCGCTACCGGGCACGCTTGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                                           <  1:2973658/137‑1 (MQ=17)

                                                tccGGCGAATCAGGACCACCAGGAGCCCAAGGAGCCCCAGGAGCCACAGGACCAACCGGACAACCAGGACTACCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                                        <  1:2820419/139‑1 (MQ=9)

                                                     ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                   <  1:857977/140‑1 (MQ=255)

                                                     ggAAGCCGGACAAGCCGGAGCCTCTGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                   <  1:336376/140‑1 (MQ=255)

                                                     cgaccccGGACCAGCCGGACCGACAGGAGCTACAGGAGCGACAGGAGCTCCCGGCGAAGCTGGACCTCCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                                   <  1:1921794/135‑1 (MQ=18)

                                                         gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTgg                                                                               >  1:1753959/1‑140 (MQ=255)

                                                         gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCACCGGGAGCGACGGGAGCGACCGGAGCAACCGGGGAAGCCGGACACACCTCAGCGACGGGAGCCACCGGGGcaccagg                                                                               >  1:2940385/1‑134 (MQ=17)

                                                           cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTTCGGGGGAAGCCCGAGCAGCCGGGGCCACGGGCGCCACAGGGGCGAGTgggg                                                                             >  1:1055547/1‑140 (MQ=21)

                                                             gACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGaa                                                                           >  2:136213/1‑140 (MQ=255)

                                                              acgtgCCGCAGCGACAGGACCCACAGGACCCCCAGGACCACCCGGCGCACCCGGACCGCCGGGAGCGAAGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGAGCCACTGGAGCTACTGGGGAAg                                                                          <  2:545271/136‑1 (MQ=2)

                                                              aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                          >  1:2506392/1‑140 (MQ=255)

                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                    >  2:212873/1‑109 (MQ=255)

                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCAc                                                                                                    <  1:212873/109‑1 (MQ=255)

                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                     <  1:2465035/140‑1 (MQ=255)

                                                                   ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                     <  1:2230864/140‑1 (MQ=255)

                                                                       agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAg                                                                 >  1:2844113/1‑140 (MQ=255)

                                                                       agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAg                                                                 >  1:99528/1‑140 (MQ=255)

                                                                        gcgactggagcgactggagggacaggtGCGAGCGGGGAAGCTGGTCGAGCGGGAGCGAGGGGTGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                <  2:1778877/140‑1 (MQ=18)

                                                                        gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGCGCGACAGGCGCTACCGGGGAAGCCGGACCACCCGGGGCGACGGGAGCCACCGGGGCGACTGGGGAGGCCGCagcagc                                                                >  2:1458999/1‑140 (MQ=18)

                                                                        gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                <  1:2215345/140‑1 (MQ=255)

                                                                            caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                                            >  1:561817/1‑76 (MQ=255)

                                                                            caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCgg                                                                                                                            <  2:561817/76‑1 (MQ=255)

                                                                                    ccaggagctacaggagcACCCGGCCCACCTGGACCTCCAGGAGCGCCGGGAGCGACAGGAGCTCCCGGGGAACCCGGACCACCCGGCGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                    <  2:442826/139‑1 (MQ=17)

                                                                                       ggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGc                                                 <  1:1596664/140‑1 (MQ=255)

                                                                                             acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                     >  2:2157071/1‑114 (MQ=255)

                                                                                             acaggagcTACCGGGGAAGCTGGACCACCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                           <  2:1411458/140‑1 (MQ=255)

                                                                                                    cTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                    >  1:2105431/1‑140 (MQ=255)

                                                                                                    cTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                    >  1:936843/1‑140 (MQ=255)

                                                                                                     tACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                                        <  1:1405159/39‑1 (MQ=40)

                                                                                                         ggggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                               >  1:87492/1‑140 (MQ=255)

                                                                                                           ggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACCGGGGCAACTGGCGAAGCCGGAGCTGCCGGAGCCACAGGAGCTACCGGCGAAGCCGGACCAg                             >  1:1963205/1‑140 (MQ=18)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCTGCCGGAGCGCCAGGAGCGACCGGGGACGCCGGACCAGc                            >  1:2996397/1‑140 (MQ=255)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACCGGGGCAGCCGGACCCGc                            >  1:234341/1‑140 (MQ=255)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                            >  1:325752/1‑140 (MQ=255)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                            >  1:500303/1‑140 (MQ=255)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                            >  1:365362/1‑140 (MQ=255)

                                                                                                            gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                            <  1:390410/140‑1 (MQ=255)

                                                                                                                    aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                    <  1:136465/140‑1 (MQ=255)

                                                                                                                    aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                    <  1:1457900/140‑1 (MQ=255)

                                                                                                                    aCCAGCCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCTGGACCAGCCGGAGCCACGGGAGCCACAGGCGCTCCAGGGGCCGGCGGACCAGCCGGAGCGACCGGAGCTCCCGGGGAAGCAGGACCAACCGGACCGa                    >  2:1129963/1‑140 (MQ=11)

                                                                                                                     ccaccaggAGCGACGGGAGCCCCCGGACCTACCGGCGAACCCGGACCCGCCGGCGCCACGGGAGCCCCAGGCGCCCCCGGCCAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                   <  2:463713/134‑1 (MQ=12)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCCACGGGAGCCACAGGGGCAACTGGTGAAGCCGGACCAGCATGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGAGctaacgta               >  1:2028305/1‑133 (MQ=17)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa               >  1:2004893/1‑140 (MQ=255)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa               >  1:702738/1‑140 (MQ=255)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa               >  1:209642/1‑140 (MQ=255)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa               >  1:2649694/1‑140 (MQ=255)

                                                                                                                         cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa               >  1:1195123/1‑140 (MQ=255)

                                                                                                                             aGCGACGGGAGCGACAGGAGCTACCGGGGCACCCGGACCACCCGGGGACACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa           <  1:655347/140‑1 (MQ=17)

                                                                                                                             aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa           <  1:2901763/140‑1 (MQ=255)

                                                                                                                             aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa           <  1:407277/140‑1 (MQ=255)

                                                                                                                                 aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg       <  1:680282/140‑1 (MQ=255)

                                                                                                                                 aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAACCGGAGCGACAgg                                                    <  1:1064432/95‑1 (MQ=255)

                                                                                                                                 aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAACCGGAGCGACAgg                                                    >  2:1064432/1‑95 (MQ=255)

                                                                                                                                  tgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                >  1:1756685/2‑82 (MQ=255)

                                                                                                                                  tgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                >  1:1632151/2‑82 (MQ=255)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACCGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg      <  2:2977548/140‑1 (MQ=255)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGCGGAACCCCGACGGCCCGGACCGACCGCAGCGCCCggg      >  2:1721963/1‑140 (MQ=14)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg      >  2:2083743/1‑140 (MQ=255)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg      >  2:1816730/1‑140 (MQ=255)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg      <  2:87492/140‑1 (MQ=255)

                                                                                                                                  cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACAGGCCGGAGCGACAGCAGCGACCggg      >  2:16941/1‑140 (MQ=17)

                                                                                                                                      aGCTACAGGAGCACCCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTTCTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg  <  2:644924/140‑1 (MQ=17)

GAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607392‑607665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`