BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,638	G→C	13.7%	A161P (GCC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,638	0	G	C	13.7%	241.6 / 3.6	119	A161P (GCC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (62/35); new base C (3/13); total (69/50)
Fisher's exact test for biased strand distribution p-value = 9.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607499‑607777

ggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACCGGGGCAACTGGCGAAGCCGGAGCTGCCGGAGCCACAGGAGCTACCGGCGAAGCCGGACCAg                                                                                                                                             >  1:1963205/1‑140 (MQ=18)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCCGGCGGAGCTTCCGGAGAGCCAGCAGCTACGGGGGAAGCCGGACCCGc                                                                                                                                            >  1:1142232/1‑140 (MQ=17)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCCGCGGAGGCACAGGGGCTCCTGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTGCCGGGGACGCGGGACCAGc                                                                                                                                            >  2:545139/1‑140 (MQ=18)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCTGCCGGAGCGCCAGGAGCGACCGGGGACGCCGGACCAGc                                                                                                                                            >  1:2996397/1‑140 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCGACCGGGGCAGCCGGACCCGc                                                                                                                                            >  1:234341/1‑140 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                            >  1:500303/1‑140 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                            <  1:390410/140‑1 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                            >  1:365362/1‑140 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                            >  1:2900951/1‑140 (MQ=255)

 gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                                                                                            >  1:2402079/1‑140 (MQ=255)

      tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGACGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                                                                                                                                       <  1:1071154/140‑1 (MQ=255)

         aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    <  1:136465/140‑1 (MQ=255)

         aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    <  1:1457900/140‑1 (MQ=255)

          ccaccaggAGCGACGGGAGCCCCCGGACCTACCGGCGAACCCGGACCCGCCGGCGCCACGGGAGCCCCAGGCGCCCCCGGCCAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  2:463713/134‑1 (MQ=12)

             gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                                <  1:136213/140‑1 (MQ=255)

             gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                                <  1:2103288/140‑1 (MQ=255)

              cTGGAGCGACGGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCCACGGGAGCCACAGGGGCAACTGGTGAAGCCGGACCAGCATGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGAGctaacgta                                                                                                                               >  1:2028305/1‑133 (MQ=17)

              cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                               >  1:209642/1‑140 (MQ=255)

              cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                               >  1:2649694/1‑140 (MQ=255)

              cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                                                               >  1:1195123/1‑140 (MQ=255)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGCACCCGGACCACCCGGGGACACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           <  1:655347/140‑1 (MQ=17)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           <  1:2901763/140‑1 (MQ=255)

                  aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                           <  1:407277/140‑1 (MQ=255)

                      aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                                                                       <  1:680282/140‑1 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACCGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  2:2977548/140‑1 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGCGGAACCCCGACGGCCCGGACCGACCGCAGCGCCCggg                                                                                                                      >  2:1721963/1‑140 (MQ=14)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  2:1816730/1‑140 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  2:87492/140‑1 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  2:2083743/1‑140 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  2:2172819/1‑140 (MQ=255)

                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACAGGCCGGAGCGACAGCAGCGACCggg                                                                                                                      >  2:16941/1‑140 (MQ=17)

                               aCAGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCCCGCGAGCCACAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                              <  2:325752/140‑1 (MQ=255)

                                      cTACCGGGGACGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                       <  1:1468268/140‑1 (MQ=255)

                                        aCCGGGGTACCCGGACCAGCCGGCGCCCCGGGTGCCACTGGGGCTCCTGGGGAAGCGGGACCACCCGGAGCGACCGGCGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                                     <  2:439270/140‑1 (MQ=17)

                                          cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCCTCCCGAGAGACAGGATCTCCCGGCCAACCCGGAGCAGCCGGATCGACAGTAGCTACCTCGGCAGCCCGACCAGCAGGAg                                                                                                   >  1:1461006/1‑140 (MQ=12)

                                          cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGTAGCGACAGGAGCTACCTGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAg                                                                                                   >  1:741284/1‑140 (MQ=255)

                                          cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                   >  1:1650965/1‑140 (MQ=255)

                                            gggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCTGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCTGAGCGACAGCAGCAACCGGGGCAGCCGGACCACCCGGAGCg                                                                                                 >  1:269915/1‑140 (MQ=18)

                                            gggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGACGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAGCAGGCCCAACCGCAGCg                                                                                                 >  1:2281177/1‑140 (MQ=18)

                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGTGGAAGCCAGACAAAACGCAGAGACAGGACCTACCGTGGAAGCCGCACCAACCGGAgacac                                                                                               >  1:2222767/1‑136 (MQ=17)

                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                               >  1:2058718/1‑140 (MQ=255)

                                              gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCGGGACCAGCCGGAGCggc                                                                                               >  2:316468/1‑138 (MQ=18)

                                              gAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGGCAACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACAGGGGCACCCGGACAATCCGGAGcagc                                                                                               >  2:557952/1‑137 (MQ=14)

                                                 gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                            >  1:1277587/1‑140 (MQ=255)

                                                          cgcGGGGCCACCGGCCCCACAGGGGCTACGGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCTACCGGCGACCCCGGACCAGCCGGCGCCACAGGAGCACCCGGGGAACCCGGAGCAACCGGAGCGACAGGAGCTACCgg                                                                                   <  2:1692674/138‑1 (MQ=14)

                                                          cccGGCGCCCCCGGAGCCACAGGGGCTCCCGGGGAACCCGGACCAGCCGCTGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCgg                                                                                   <  2:2475652/139‑1 (MQ=11)

                                                                     gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGCACCCGGACCACGCGGAGCGACAGGAGCGACCGGGGCCGCCggcg                                                                        >  1:1844969/1‑138 (MQ=14)

                                                                       gAGCCACAGGCGCTACTGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCa                                                                      <  1:545139/140‑1 (MQ=255)

                                                                         gCCACAGGCGCTTCTGCGCCAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                    <  2:479936/140‑1 (MQ=11)

                                                                         cccACAGGGCCTACCGGCGAAGCCGGACCCGCCGGACCCACCGGAGCTACCGGGGCAGCCGGACCATCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc                                                                    <  2:583886/139‑1 (MQ=11)

                                                                          ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGTCCAACCGGAGCGACAGGAGCGACCGGGGCAGCAGGAGCAGcc                                                                   >  1:1489782/1‑140 (MQ=14)

                                                                            acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGCCAGGAGCTACCGGCGAAGCCGGACCAGCCgg                                                                 >  2:1654315/1‑140 (MQ=255)

                                                                            acaGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGCCGCCGGCGCTGCCgg                                                                 >  1:407559/1‑140 (MQ=11)

                                                                             caGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGTATCTACCGGGGAAACAGGAGCAGCCGGa                                                                >  2:1317165/1‑140 (MQ=14)

                                                                              aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCGACGGGGGCCGCGGGCCCAACCGGAGCGGGCGGAGCGACCGCGGGCGCGGGACCAGCCGGAg                                                               >  1:2508956/1‑140 (MQ=14)

                                                                                   cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAcc                                                          >  2:2019191/1‑139 (MQ=40)

                                                                                   cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                          >  2:1007326/1‑140 (MQ=40)

                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACTCCGGAACACCAGTAGCGACAGGAGATACCGGTGACGCCGGACCAACCGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCGACTgg                                                        >  1:1463862/1‑140 (MQ=11)

                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGCGCATCCGGACCGACAgg                                                        >  1:1463747/1‑140 (MQ=14)

                                                                                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGTCAgg                                                        >  1:1126645/1‑140 (MQ=255)

                                                                                       tGGGGAATACGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                      <  1:1615874/140‑1 (MQ=255)

                                                                                       tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                      <  2:1469157/140‑1 (MQ=40)

                                                                                       cggcgAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                      <  1:1266019/136‑1 (MQ=255)

                                                                                           gAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAACCCGGCCCAGCCGGAGCGGCCGGAGCGGCCGGCGAGGCCGGCGCAACCGGAGCTACGGGAGCGACCGGAGGCTCCGGAGCAACCGGCGCGGCAGGAGcgcg                                                  >  2:1504615/1‑137 (MQ=2)

                                                                                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAACCCGGACCACCCGGAGCGCCAGGAGCGACCGGGGCAGCCGGACAAGCCGGAGCGACAGGAGCTCCCgg                                               >  2:39827/1‑140 (MQ=14)

                                                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCGACCGGCGCCACCGGCCCAGCCGGAGCGACGGGAGCCACCggcg                                             >  2:793298/1‑138 (MQ=11)

                                                                                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCCACCgggg                                             >  2:1567285/1‑140 (MQ=255)

                                                                                                   agcccccGGAGCCCCAGGCGCACCCGGCGCACCCGGACCACCCGGAGCCCCAGGCGCTCCCGGCGAAGCCGGAGCCACCGGCGCGCCAGGAGCTACCGGGGAAGCCGGACCCGCCGGCGCGACAGGAGCTACCGGGGAAg                                          <  2:1237886/135‑1 (MQ=2)

                                                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcg                                                                                              <  2:1019884/88‑2 (MQ=17)

                                                                                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                              >  1:1019884/1‑88 (MQ=35)

                                                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTAc                                                                                      >  1:1074186/1‑91 (MQ=37)

                                                                                                        ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTAc                                                                                      <  2:1074186/91‑1 (MQ=37)

                                                                                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                 >  2:1912545/1‑140 (MQ=255)

                                                                                                                  aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCGACCGGGGACTCCGGACCAAACTGAGCGACAGGAGCTACCGGGGCCGCCGGACCAGCACGAGCGAGAGGAGCTACCGGGGACGCCGGACCAACCGGAg                           >  2:2821541/1‑140 (MQ=14)

                                                                                                                  aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGCCGCCGGACCAGCCGGAGCGGCAGGAGCTAGCGGGGCACCCGGGGCATCCGGAg                           >  2:2563897/1‑140 (MQ=14)

                                                                                                                    gAGCGACCGGCGCACCCGGACCACCCGGAGCCCCCGGAGCTACCGGGGACCCGGGAGCAACGGGTGCCACAGGAGCTACCGGTGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                         <  1:1517677/140‑1 (MQ=2)

                                                                                                                      gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAc                       >  2:2775795/1‑140 (MQ=255)

                                                                                                                      gCTACCGGGGAACCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                       <  1:803537/140‑1 (MQ=255)

                                                                                                                      gCTACCGGGCACGCCGGACCCCCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                       <  2:936843/140‑1 (MQ=14)

                                                                                                                      ccTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCACCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGCAGCGAc                       <  1:1832273/139‑1 (MQ=11)

                                                                                                                         cccGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    <  1:2451858/139‑1 (MQ=255)

                                                                                                                         cccGGCGATGCAGGAGCACCCGGACCACCAGGACCTACCGGCGAACCCGGACCAACCGGCCCCACAGGACCACCCGGCGAACCGGGACCCTCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    <  1:251410/139‑1 (MQ=9)

                                                                                                                         aCCGGGGAAGCCGGACCAGCCGGAGCGACAGTAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                                   <  2:554044/125‑1 (MQ=38)

                                                                                                                         aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                                   >  1:554044/1‑125 (MQ=38)

                                                                                                                          ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGGCCGGa                   >  2:1211143/1‑140 (MQ=255)

                                                                                                                           cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCGGGTCAAGCCGGAGCCACAGGATCCCACGGGGCTGCGGGACCCGCCGGCGCGGCGGCAg                  >  2:462374/1‑140 (MQ=11)

                                                                                                                               gctgCCGGACCACGCGGACCGACATGATCTGGGGGGCAAGCCGGTCCCACGTGAGCGAGAGGTGGTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  2:2028305/137‑1 (MQ=9)

                                                                                                                               gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              >  1:2453924/1‑140 (MQ=255)

                                                                                                                                aacccGGACCACCCGGAGAGACAGGAGCTACCGTGTTAGCCGGACCACCGGGAGCTACTGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca             <  1:2760364/137‑1 (MQ=11)

                                                                                                                                  gCCGGACCCGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:209642/140‑1 (MQ=255)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                           >  1:586422/1‑60 (MQ=33)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                           <  2:13671/60‑1 (MQ=33)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                           <  2:586422/60‑1 (MQ=33)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGa                                                                                           >  1:1578967/1‑60 (MQ=33)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCACCCGGACCAACCGGAGCGACGGGAGCCACAgg           >  2:1223252/1‑140 (MQ=255)

                                                                                                                                  gCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCATCCGCAGCTACAGGAGCAACCGGGGCCGCCGGACCAGCCGGAGCGACCGGCGCTCCCGCGGACGGCGGGGCCTCCGGCGCGACGGGAGccgcggg           >  1:2636641/1‑135 (MQ=2)

                                                                                                                                  gCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:702738/140‑1 (MQ=255)

                                                                                                                                  gCCGGACCACCCCGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:1195123/140‑1 (MQ=14)

                                                                                                                                    cGGCCCCGCCGGAGCGACTGGAGCTCGCGGTGCCGCGGGAGCCCCCGGTGCGTCAGGCCCACCCGGGGACGCGGGACCAGCGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAgggg         <  2:2527904/140‑1 (MQ=9)

                                                                                                                                           gCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTgg  <  1:1912545/140‑1 (MQ=255)

GGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607499‑607777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`