BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,838	A→G	5.1%	T227T (ACA→ACG)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,838	0	A	G	5.1%	758.2 / 17.6	235	T227T (ACA→ACG)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (102/117); new base G (5/7); total (109/126)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTA  >  NZ_CP034943/607699‑607974

acgtcggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                          >  2:121276‑M2/17‑140 (MQ=255)

  gtcggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                        >  2:70625‑M2/15‑140 (MQ=255)

  gtcggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGACAgg                                                                                                                                        >  2:1949968‑M2/15‑140 (MQ=255)

  gccggaccagccggACCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGCCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                                                                        <  1:2921677‑M2/126‑1 (MQ=255)

  cccggcccacccggCGCGACCGGAGCAACCGGCGAAGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                                                                        <  1:1478558‑M2/126‑1 (MQ=255)

  cccggacccccgggAGAGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCACCGGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                                                                        <  2:2580408‑M2/126‑1 (MQ=255)

    cggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAGGAg                                                                                                                                      >  2:1361544‑M2/13‑140 (MQ=255)

    cggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                      >  2:1542470‑M2/13‑140 (MQ=255)

    cggaccagccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                      >  1:1643962‑M2/13‑140 (MQ=255)

          agccggAGCGACAGGAGCTACGGGAGAACCCGGAGCACCCGGAGCGACCGGAGCCCCAGGAGCTACCGGGGACGACGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGGGCCACgg                                                                                                                                <  1:2244960‑M2/134‑1 (MQ=255)

           gccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACggg                                                                                                                               >  2:1454252‑M2/6‑140 (MQ=255)

           gccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                                                                               >  2:434072‑M2/6‑140 (MQ=255)

           gccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACagg                                                                                                                               >  2:88707‑M2/6‑140 (MQ=255)

           gccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCAAggg                                                                                                                               >  2:2710397‑M2/6‑140 (MQ=255)

            ccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                              >  1:971089‑M2/5‑140 (MQ=255)

            ccggAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                                                              >  2:543633‑M2/5‑140 (MQ=255)

             cggCGCGACAGGCGCGACCGGGGAAGCCGGACCACCCGGCGCCACCGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGCAGCGACAGGCGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCacagggg                                                                                                                             <  1:630604‑M2/137‑3 (MQ=255)

                aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGcta                                                                                                                          <  2:584855/140‑3 (MQ=255)

                  cGACCGGAGCTACCGGCGAAGCCGGACCACCGGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGTCCAGCCGGAGCGAGTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcaca                                                                                                                        <  1:2077710/140‑1 (MQ=17)

                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                     >  1:37697/1‑140 (MQ=255)

                     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                     >  2:1588026/1‑140 (MQ=255)

                          gCTCCCGCGGCAGCCCGCGCACCCGCAGCGACGGGTGGCACCGCGGCTACTGGGCACGGCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                <  2:1984648/140‑1 (MQ=18)

                          gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                >  1:597515/1‑140 (MQ=255)

                          gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                <  1:2756720/140‑1 (MQ=255)

                           cTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGa                                                                                                               >  2:1329373/1‑140 (MQ=255)

                             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                             >  2:2920124/1‑140 (MQ=255)

                              ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagca                                                                                                            >  2:2141282/1‑140 (MQ=255)

                               tggaccaGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                           >  1:1400428/7‑140 (MQ=18)

                               cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                           <  1:1199238/140‑1 (MQ=255)

                                   gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTgg                                                                                                       >  2:944956/1‑140 (MQ=14)

                                   gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                <  1:1359969/131‑1 (MQ=255)

                                   gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCCACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGGAGCCGCTgg                                                                                                       >  2:1680447/1‑140 (MQ=255)

                                    aacccGGACCAACCGGCGCGACGGGAGCGACAGGTGATACTGGGGTCGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                      <  2:37697/137‑1 (MQ=21)

                                     aGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                     <  1:3456/140‑1 (MQ=255)

                                     aGCCGGAACAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGt                                                                                                     <  1:1654315/140‑1 (MQ=255)

                                           aCCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                                                                               >  2:82288/1‑140 (MQ=255)

                                           aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                                                                               >  1:2292987/1‑140 (MQ=255)

                                               cccGGCGCGACGGGAGCCACAGGGGCTTCTGGCGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  2:2506392/139‑1 (MQ=255)

                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  1:828103/140‑1 (MQ=255)

                                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  1:122867/140‑1 (MQ=255)

                                                ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                          >  2:163802/1‑140 (MQ=255)

                                                ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                          >  1:1720010/1‑140 (MQ=255)

                                                 cGGAGCGCCGGGAGCGACAGGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACg                                                                                         <  2:1578238/140‑1 (MQ=255)

                                                 cGGAGCGACGTGAGCCACCGGTGAGACTGGGGTCGGCGGAGCAGGAGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACg                                                                                         <  2:1712357/140‑1 (MQ=25)

                                                    accGACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAAACGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  1:540568/138‑1 (MQ=255)

                                                    aGCGTCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  1:2602579/140‑1 (MQ=255)

                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATATGGGt                                                                                      <  1:1567285/140‑1 (MQ=255)

                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  1:464540/140‑1 (MQ=255)

                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  2:1821738/140‑1 (MQ=255)

                                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  1:1443250/140‑1 (MQ=255)

                                                         cGGGAGCGACAGGCGCTACGGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatata                                                                                 <  1:1325664/140‑1 (MQ=21)

                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  2:379256/130‑1 (MQ=255)

                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           >  1:379256/1‑130 (MQ=255)

                                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatata                                                                                 <  1:2034627/140‑1 (MQ=255)

                                                                    ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                      <  1:1775705/140‑1 (MQ=255)

                                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatata                                                                                 <  2:1143996/125‑1 (MQ=255)

                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                <  2:108210/140‑1 (MQ=255)

                                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                >  1:1859737/1‑140 (MQ=255)

                                                                           cTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  1:772071/112‑1 (MQ=255)

                                                                             ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                             >  1:934358/1‑140 (MQ=255)

                                                                               ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                           <  1:819978/140‑1 (MQ=255)

                                                                               ggACGACGGACCAGCGGGAGCGACAGGTGCTACCGGGGAAGCAGGTCCATCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                           <  2:1867508/140‑1 (MQ=38)

                                                                                gACGTCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                          <  1:561263/140‑1 (MQ=255)

                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                          <  1:1317165/140‑1 (MQ=255)

                                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                          >  1:3027456/1‑140 (MQ=255)

                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                      >  1:2524919/1‑93 (MQ=255)

                                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                      <  2:2524919/93‑1 (MQ=255)

                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAg                                                                                                                             <  2:2026300/68‑1 (MQ=255)

                                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                  <  2:1398050/95‑1 (MQ=255)

                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                          >  2:3004315/1‑132 (MQ=255)

                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATag                                                  >  2:783866/1‑140 (MQ=255)

                                                                                        aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATag                                                  >  2:2213312/1‑140 (MQ=255)

                                                                                          cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATagag                                                >  1:2503822/1‑140 (MQ=255)

                                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                             >  2:3054829/1‑140 (MQ=255)

                                                                                                    caCAGGAGCTACCGGGGAAGCCGGACCACCCGGCGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      <  2:2803962/91‑1 (MQ=255)

                                                                                                    caCAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                      >  1:2803962/2‑92 (MQ=255)

                                                                                                           gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGa                               <  2:2348858/140‑1 (MQ=255)

                                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCCGCTGGGTTATCCGAATACGGGTATATAGATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTc                            >  2:2965929/1‑140 (MQ=255)

                                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTc                            >  2:2890840/1‑140 (MQ=255)

                                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                          <  1:2111471/140‑1 (MQ=255)

                                                                                                                cGGGGAAGCCGGACCAACCCGAGCGACAGGCGCCCCGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                          <  1:3105626/140‑1 (MQ=255)

                                                                                                                       gCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGAt                   >  1:2170844/1‑140 (MQ=255)

                                                                                                                          ggAGCATCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                <  1:2404703/140‑1 (MQ=255)

                                                                                                                            aCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca              >  1:1040151/1‑140 (MQ=255)

                                                                                                                              cacccGGCGCGACAGGCGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATTCGGGTATATATATAACTTAAGCGCTGAAACGGTAGCTATAGAGGATGCTGTCATTTTTGATTCAACAGGGATAtgcacc            <  2:1592284/137‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGTCACAGGAGCAGCTGGGTTATCCGAATACGTGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:2539642/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATCGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  2:601299/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  2:102223/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:19020/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:2657240/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:1130179/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGATATAGCGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:239502/140‑1 (MQ=255)

                                                                                                                                aCCGGAGCGACACGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGACGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc          <  1:1109260/140‑1 (MQ=255)

                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           >  2:2546968/1‑54 (MQ=255)

                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  1:1510237/54‑1 (MQ=255)

                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           >  2:1510237/1‑54 (MQ=255)

                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa     >  1:1353813/1‑140 (MQ=255)

                                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa     >  1:2883059/1‑140 (MQ=255)

                                                                                                                                      gCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                           <  1:1983297/53‑1 (MQ=255)

                                                                                                                                        gACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTa  <  2:2323162/140‑1 (MQ=255)

AAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTA  >  NZ_CP034943/607699‑607974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`