Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 704,769 G→C 5.0% E136D (GAG→GAC EO946_RS03655 → phosphotransferase enzyme family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943704,7690GC 5.0% 579.8 / 16.2 172E136D (GAG→GACEO946_RS03655phosphotransferase enzyme family protein
Reads supporting (aligned to +/- strand):  ref base G (76/87);  new base C (4/5);  total (80/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

AAGACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATTCTCTG  >  NZ_CP034943/704633‑704907
                                                                                                                                        |                                                                                                                                          
aaGACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGAtc                                                                                                                                         >  2:1716378/1‑139 (MQ=255)
aaGACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATg                                                                                                                                         >  2:713852/1‑140 (MQ=255)
aaGACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATg                                                                                                                                         >  2:675534/1‑140 (MQ=255)
  gACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGca                                                                                                                                       <  1:3009629/140‑1 (MQ=255)
  gACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGca                                                                                                                                       <  2:1940498/140‑1 (MQ=255)
      ttGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGc                                                                                                                                   <  1:1683268/140‑1 (MQ=255)
        gaagaaGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCt                                                                                                                                 >  2:961104/1‑140 (MQ=255)
        gaagaaGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCt                                                                                                                                 >  2:2272462/1‑140 (MQ=255)
              gTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACaaa                                                                                                                           <  1:679608/140‑1 (MQ=255)
              gTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACaaa                                                                                                                           <  2:1332598/140‑1 (MQ=255)
              gTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACaaa                                                                                                                           <  1:251115/140‑1 (MQ=255)
                   ggATGGAACCGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  2:133525/140‑1 (MQ=255)
                   ggATGGAACCGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  2:2017094/140‑1 (MQ=255)
                   ggATGGAAACGGCTGTTCATTTTTACTGAGAGTGTATGAGAACGCGCCGGGGCAAAAAGTGGAAGCATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  2:2460466/140‑1 (MQ=255)
                   ggATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGTCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  1:2441056/140‑1 (MQ=255)
                   ggATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  1:996690/140‑1 (MQ=255)
                   ggATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  2:2771211/140‑1 (MQ=255)
                   ggATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCt                                                                                                                      <  1:1588106/140‑1 (MQ=255)
                      tGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATc                                                                                                                   >  2:1107089/1‑140 (MQ=255)
                      tGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATc                                                                                                                   >  2:324292/1‑140 (MQ=255)
                        gAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:3111388/140‑1 (MQ=255)
                        gAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:2715188/140‑1 (MQ=255)
                        gAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:1568950/140‑1 (MQ=255)
                        gAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:2443853/140‑1 (MQ=255)
                        gAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  2:259529/140‑1 (MQ=255)
                         aaaCGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTCTTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAAc                                                                                                                >  1:1775419/1‑140 (MQ=255)
                             ggcggTTCATTTTTACTGAGCGTGTATGAGACAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGGAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAg                                                                                                            <  2:388324/140‑1 (MQ=255)
                             ggcggTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAg                                                                                                            <  1:2596204/140‑1 (MQ=255)
                              gcggTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGCCTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGc                                                                                                           >  1:1190039/1‑140 (MQ=255)
                              gcggTTCATTTTTACTGAGAGTGTATGAGAAAGAGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTAGGTGCTTGTCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGc                                                                                                           >  1:1396735/1‑140 (MQ=255)
                                  ttCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAAcc                                                                                                       <  2:2339458/140‑1 (MQ=255)
                                  ttCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAAcc                                                                                                       <  2:498483/140‑1 (MQ=255)
                                  ttCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAAcc                                                                                                       <  2:3039379/140‑1 (MQ=255)
                                                gagTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGa                                                                                         <  2:2882141/140‑1 (MQ=255)
                                                   tgtATGAGAAAGCGCCGGGGCAAAAAGTGGATGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCAGCATGCACAGCCTGACAAAAAGCTACAAATTGAGCAATCCCGCTTTCAAAAGACAg                                                                                      >  1:1661345/1‑140 (MQ=255)
                                                   tgtATGAGAAAGCGCCGGGGCAAAAAGTGGATGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCAGCATGCACAGCCTGACAAAAAGCTACAAATTGAGCAATCCCGCTTTCAAAAGACAg                                                                                      >  1:399205/1‑140 (MQ=255)
                                                     tATGAGATAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTTGTATGAAACGCTATTTTATGAGCTTGGCAGATTCACAGGCGAGATGCACAGCCTGTCAAAAAGCTATCAACTGAGCAACACTGCTTTTAAAATACAGGa                                                                                    <  1:2300214/140‑1 (MQ=255)
                                                       tGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAAt                                                                                  <  2:985876/140‑1 (MQ=255)
                                                       tGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAAt                                                                                  <  2:837443/140‑1 (MQ=255)
                                                        gagaAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATg                                                                                 <  2:1124002/140‑1 (MQ=255)
                                                         agaAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATgg                                                                                >  1:1034224/1‑140 (MQ=255)
                                                           aaaGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGa                                                                              <  2:2671312/140‑1 (MQ=255)
                                                           aaaGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGa                                                                              <  2:1503879/140‑1 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATg                                                                            >  1:1360555/1‑140 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATg                                                                            >  1:745967/1‑140 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTGTTAAAAGACAGGAATGGGATg                                                                            >  2:101707/1‑140 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGAGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATg                                                                            >  2:1774633/1‑140 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTCGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATg                                                                            >  1:1141680/1‑140 (MQ=255)
                                                             aGCGCCGGGGCAAAAAGGGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTGTTAAAAGACAGGAATGGGATg                                                                            >  2:908553/1‑140 (MQ=255)
                                                               cgcCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGaa                                                                          >  1:1458070/1‑140 (MQ=255)
                                                               cgcCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGaa                                                                          >  1:761602/1‑140 (MQ=255)
                                                               cgcCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGaa                                                                          >  1:1495051/1‑140 (MQ=255)
                                                               cgcCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGaa                                                                          >  2:1506259/1‑140 (MQ=255)
                                                               cgcCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGAGACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAACAGACAGGAATGTGATGaa                                                                          >  1:2328305/1‑140 (MQ=255)
                                                                 ccGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAaga                                                                        >  2:2937734/1‑140 (MQ=255)
                                                                    gggCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAgagga                                                                     <  1:450152/140‑1 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:1818917/84‑1 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 <  1:261113/84‑1 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 >  2:1818917/1‑84 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCaa                                                                                                                 >  2:261113/1‑84 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCt                                                         <  1:779564/140‑1 (MQ=255)
                                                                                gaagaaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCt                                                         <  1:2974211/140‑1 (MQ=255)
                                                                                    aaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCGTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTgaga                                                     <  1:2715550/140‑1 (MQ=255)
                                                                                    aaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTgaga                                                     <  2:1574494/140‑1 (MQ=255)
                                                                                    aaTCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTgaga                                                     <  2:745967/140‑1 (MQ=255)
                                                                                           cTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTAtg                                              <  1:2675792/140‑1 (MQ=255)
                                                                                                 tGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCg                                        <  1:268678/140‑1 (MQ=255)
                                                                                                 tGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCg                                        <  1:2087152/140‑1 (MQ=255)
                                                                                                      cGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGAt                                   >  1:2483287/1‑140 (MQ=255)
                                                                                                      cGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGAt                                   >  1:1939146/1‑140 (MQ=255)
                                                                                                      cGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGAt                                   >  1:891101/1‑140 (MQ=255)
                                                                                                      cGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGAt                                   >  1:2764782/1‑140 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATcc                                 >  1:1759587/1‑139 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCa                                 >  2:2541117/1‑140 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCa                                 >  1:165387/1‑140 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCAGCATGCACAGCCTGACAAAAGGCTACAAATTGAGCAATCCCGCTTTCAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAATATGTGCCTGAAGATCa                                 <  2:399505/140‑1 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCAGCATGCACAGCCTGACAAAAAGCTACAAATTGAGCAATCCCGCTTTCAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAATATGTGCCTGAAGATCa                                 <  2:2352105/140‑1 (MQ=255)
                                                                                                        cTATTTTATGAGCTTGGCAGATACACAGGCAGCATGCACAGCCTGACAAAAAGCTACAAATTGAGCAATCCCGCTTTCAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAATATGTGCCTGAAGATCa                                 <  2:1661345/140‑1 (MQ=255)
                                                                                                         tATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAg                                >  2:540326/1‑140 (MQ=255)
                                                                                                         tATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAg                                >  2:1445326/1‑140 (MQ=255)
                                                                                                         tATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAg                                >  2:2994965/1‑140 (MQ=255)
                                                                                                         tATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAACAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAg                                >  2:2935991/1‑140 (MQ=255)
                                                                                                                  aGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGgtg                       >  2:1894094/1‑140 (MQ=255)
                                                                                                                   gCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGgtgt                      >  2:2294615/1‑140 (MQ=255)
                                                                                                                   gCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGgtgt                      >  1:2161170/1‑140 (MQ=255)
                                                                                                                     ttGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTgaga                                                     <  2:156032/107‑1 (MQ=255)
                                                                                                                     ttGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTgaga                                                     >  1:156032/1‑107 (MQ=255)
                                                                                                                     ttGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGttt                    >  2:726977/1‑140 (MQ=255)
                                                                                                                       ggCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAGAGTCTGTGGCCGAAGATCAGACAATGGTGTTTct                  >  2:1900539/1‑139 (MQ=255)
                                                                                                                         cAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGc                <  1:2272462/140‑1 (MQ=255)
                                                                                                                          aGATACACAGGCGAGATGCACAGCCTGACAAACAGCTATCAACTGAGCAACCCTGCTTTTAACAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCa               <  1:480385/140‑1 (MQ=255)
                                                                                                                          aGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCa               <  1:617918/140‑1 (MQ=255)
                                                                                                                                 caGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGa                                                                                         >  2:1465961/1‑59 (MQ=255)
                                                                                                                                 caGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGAt        >  2:2129191/1‑140 (MQ=255)
                                                                                                                                  aGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGAtt       >  1:1004284/1‑140 (MQ=255)
                                                                                                                                    gCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACaaa                           >  2:2003868/1‑118 (MQ=255)
                                                                                                                                    gCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATtct     >  2:767120/1‑140 (MQ=255)
                                                                                                                                    gCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATtct     <  1:3015265/140‑1 (MQ=255)
                                                                                                                                    gCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATtct     >  1:2398258/1‑140 (MQ=255)
                                                                                                                                       agaTGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATTCTCtg  >  2:3071955/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AAGACGTTGAAGAAGTGCCGGATGGAAACGGCGGTTCATTTTTACTGAGAGTGTATGAGAAAGCGCCGGGGCAAAAAGTGGAAGAATCGGACTGGAATGAAACGCTATTTTATGAGCTTGGCAGATACACAGGCGAGATGCACAGCCTGACAAAAAGCTATCAACTGAGCAACCCTGCTTTTAAAAGACAGGAATGGGATGAAGAGGAGCAATTAAAGCTGAGAAAGTATGTGCCCGAAGATCAGACAAAGGTGTTTCAGCAAGCCGATTCTCTG  >  NZ_CP034943/704633‑704907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: