Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 10.2% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC10.2% 375.3 / 3.8 160Q22H (CAA→CACEO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (102/31);  new base C (3/20);  total (105/55)
Fisher's exact test for biased strand distribution p-value = 7.81e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGG  >  NZ_CP034943/755392‑755667
                                                                                                                                            |                                                                                                                                        
cACCGTTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGTAATGAGAAATGGCAAATCACATTTATCTTGCTGTCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                          <  2:1370650/140‑1 (MQ=255)
cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                          >  1:2869532/1‑140 (MQ=255)
  ccGTTTTTTTCATGGGAAGAGCTGTCCATTATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                        <  1:1073980/140‑1 (MQ=255)
      ttttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgggggg                                                                                                                                    >  2:1720879/1‑140 (MQ=255)
            cAAGGGAATAGCTTTCCATGATTGATGCCATATGAAACGAAAAATACGAGGGGTAATGAGAAATGGCAAATCACATTTTTCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGAACAAATCACGGGGGGTggggg                                                                                                                              <  2:622942/140‑1 (MQ=255)
             tatttatgagaTGTCCATTATTGATGCAATTTGAAATTAAAAAATTGATGGTTTATTATAAATGTCAATTCAATTTTTTCTTGCTGGCGATTCAACTTTTCTAACTTATGGATACAGCACAAATCAAGGGGGGTGGGGGc                                                                                                                             <  2:1299139/133‑1 (MQ=255)
                       ctttCCATTCTTGATGCCAAATGAAACCAAAAAAACGAGGGGGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGACACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCgg                                                                                                                   <  1:1072244/137‑1 (MQ=255)
                          tCCATGCTTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                >  1:342903/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAACTGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACCAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                >  1:1875041/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                >  1:1287292/1‑140 (MQ=255)
                                   tatGCCATATGAACAGAAAAAAAGGAGGGGGAATGAGAAATGTCAAATCACATTTATCTTTCTGGCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                       <  2:2511522/139‑1 (MQ=255)
                                   gatGCCATATGAAACGAAAAAAACGACGGGGAATGAGAAATGCCAAATCACATTTATCGTGCGGGCGATTCAACTGTTCAACCGTATGGAGACAGCACAACACAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                       <  1:153168/140‑1 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGTGGGACTGAGAAATTGCAAATCACATTTATATTGCTGGAGATTCAACTGTTCAAACGTATGGATTCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTTCCg                                                                                                      >  2:2722928/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGTGAATGAGAAATGGCAAATCACATTTATCTTTCTGTCGATTAAACTGTTCAATCGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATATGCCg                                                                                                      >  2:118635/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAATCGTATGGAGACAGCACATATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  2:2036555/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:2286957/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:1215695/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:560010/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:649389/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:772609/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:2454980/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  2:2621115/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:1273092/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  2:309393/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGGTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  2:1528161/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACAGTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  2:386884/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATCGAAAATCACATTTTTCTTGCTGGCGATTCAACTGTTCAAACGAACGGAGACAGCACAACTCCCGGCGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      <  2:620927/140‑1 (MQ=255)
                                      gCCATATGAAACCAAAAAAACGAGGGCGAATGAGAACGTGCAAATCACATTGATCTTGCTGGCGATTAAACTGTTCAAACCTATCGAGACAGCACAACTCCAGGGGGCTCGGGCCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                    <  1:1027689/140‑1 (MQ=255)
                                      cccacacgAAACGACAAAAACGAGGGGGACTGAGAAATGGCAAAACACAGTTATCTTGCTGGCGATTCACCTCTTCAAACGTATCGAGACAGCACAAAACAAGGCGGGTGCGGGCAGTGTCTCGGCTCGCATCTGCCGGa                                                                                                    <  1:2464925/133‑1 (MQ=255)
                                        cATATGAAAATAAAAAATGTTGGTGGAATGAGATATGGCAAATCAGATTTATCTTGCTGGCGTTTATACTGTTGAATCGTATGGAGACAGCACAAATCAAGGGGGGTTGGGGCAGTTTCTCGGCTCGCATCTGCCTGAGc                                                                                                  <  2:1685900/140‑1 (MQ=255)
                                          tatGAAACGAAAAAAACGATGGGGAATGAGAAATGGCAAATCACAATTATATTGATGGCGATTCAACTGTTCAATCGTATGGAGACAGCACAAATCAAGGGGGGTGTGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                >  2:2467215/1‑140 (MQ=255)
                                           atGAAACGAAAAAAACGAGTGTGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGACACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                               <  2:1915292/140‑1 (MQ=255)
                                           atGAAACGAAAAAAACGAGGGTGAATGAGAAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGCTGGGGCCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                               <  2:815006/140‑1 (MQ=255)
                                           atGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCata                                                                                               >  2:2481106/1‑140 (MQ=255)
                                              ataCGAAAAATACGAGTGGTTATGATAAATGTAAAATGTCTTTTATCTTGCTGGCGTTTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                            <  2:1939270/138‑1 (MQ=255)
                                              aaaCGATAATAACGTGGGGGAATGTGAAATGGCAAATCTCATTTTTCTTGCTGGCTATTCAAGTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGCCAGTTTCTCGGATCGCATCTGCCGGAGCATATTc                                                                                            <  2:2763489/140‑1 (MQ=255)
                                              aaaCGACAAAAACGAGGCTGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAACCGTATGGAGACACAACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                            <  1:998581/140‑1 (MQ=255)
                                              aaaCGAAAAAAACGATGGGGAATGATAAATGTCAAATCACATTTATCTTGCTGGCGATTCAACTTTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                            <  2:2067/140‑1 (MQ=255)
                                               tgtataaaaaaCGAGGAGGAATGAAAAAATGGCAAATCATATTTATCTTGCCGGCGATTCGACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGCTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                            <  1:2242626/135‑1 (MQ=255)
                                                aCGAAAAAAACGCGGGGGAATGAGCACTCCCAAATCACCTTTATCTTGCTGGCGATACAACTGCTCAACCGTATGCCGCCAGCACAAAGCACGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                          <  1:2876550/140‑1 (MQ=255)
                                                aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                          >  1:421836/1‑140 (MQ=255)
                                                          cgatgttgAATGATAAATGGAAAATCACATTTATCTTGCTGGCTCTTCAACTGTTCAAACGTATTGAGACAGCAAAAATCAAGGGTGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACa                                                                                <  2:806930/133‑1 (MQ=255)
                                                               tgttaTGATAAATTGAAAATGACAGTTATCTTTCTGGCGATTCAACTTTTCAACCGTATGGATACAGCAAAATTCATGGGGGGTGGGGGAATTTTCTCTGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagagga                                                                           <  2:823305/136‑3 (MQ=255)
                                                                ggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACCCCACAAATCAAGGGGGGTGGGGCCAGTGTCTCGGCTCTCATCTGCCGGATCATCTTCAAGTGATTAACAGAGCaa                                                                          >  2:2451606/1‑140 (MQ=255)
                                                                 gactGAGAAATGGCAACTCTCCTTTATCGTGCTGGCCATTCAACGGTACAACCGTATGCACACAGCACACAGCCCGGGGGGGGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAt                                                                         <  1:72707/137‑1 (MQ=255)
                                                                  gatggtgaAATGGCAAATCACATTTTTCTTTCTGGCTATTCAACAGTTCAAAGGTTTGGAGCGAGCACATATGATGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAt                                                                         <  2:1300145/134‑1 (MQ=255)
                                                                     gagaAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCggg                                                                     >  2:1873208/1‑140 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAATCGTATGGAGACCGCAGAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                              >  1:881143/1‑140 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                              >  1:2398149/1‑140 (MQ=255)
                                                                             ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAAAGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGGTGCTCGGCTCGCATCTGCCGGAGCATATTCACGTGATTCACAGAGCACTCGGGGgcagac                                                              >  1:2405280/1‑139 (MQ=255)
                                                                                 aaTCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAAAAGAGCAATCGGGGGAAGAAGCTc                                                          >  2:443222/1‑140 (MQ=255)
                                                                                   tCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACCGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTCACAGAGCACTCGGGGGAAGAAGCTCGa                                                        >  2:1584529/1‑140 (MQ=255)
                                                                                    caccTTTATCTTGCTGGCGATTCAACTGTTCAACCGTCGCGAGACAGCACACAACCCGGGGGGGGCGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaa                                                       <  1:2349172/140‑1 (MQ=255)
                                                                                    cacaTTTCTCTTGCTGGCGCTTCCACAGTACAACCCGCGGGACACAGCACACATCCAGCGCGGTGGCGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGCGCAATCGGGGGAAGAAGCTCGaa                                                       <  1:191035/140‑1 (MQ=255)
                                                                                    cacaTTTATCTTGCTGGCGATTCAACTGTCCAAACGCCTCCAGCCAGCACAACCCCCGGGGGCGGGCCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGaa                                                       <  1:1109333/140‑1 (MQ=255)
                                                                                       aTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGAGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                    <  1:2729538/140‑1 (MQ=255)
                                                                                       aTTTATCTTGCGTGCTATTCCACTGTTCTTAGGCATGCAGACAGCACACCTCCCGGGGGGTGGGCCCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                    <  2:392309/140‑1 (MQ=255)
                                                                                         atATCTTGCTCGCGACTCAACTGATCCACCGTCTCGCCACACCACAAAACCCGGGGCGGCGCGGCAGGTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAt                                                  <  1:857603/139‑1 (MQ=255)
                                                                                            ttgtGCTGGCGATTCAACTTTTCATACTTATGGTGAGTGAACCAATCTTGGGGGGTGGGCGAAGTTTCTCGGCTCGCATCTGCCGGATCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTtg                                               <  2:916849/137‑1 (MQ=255)
                                                                                             cTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTtgt                                              >  1:1218384/1‑140 (MQ=255)
                                                                                             cTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTtgt                                              >  1:167176/1‑140 (MQ=255)
                                                                                             cTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTtgt                                              >  1:698935/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAATGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACAGTTGTGGaa                                          >  1:3029522/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGag                                          >  2:2253372/1‑139 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                          >  1:2603376/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                          >  2:2816877/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                          >  2:2816889/1‑140 (MQ=255)
                                                                                                 cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                          >  1:1008487/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                           >  1:1383705/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                           >  1:522943/1‑140 (MQ=255)
                                                                                                                ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                           >  1:523153/1‑140 (MQ=255)
                                                                                                                      cctctGGAGACAGCACACATCCAGGTGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGCGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGAtt                     <  2:2801174/136‑1 (MQ=255)
                                                                                                                      cGTATGGAGCCACCCCCAATCTCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGAtt                     <  2:146000/140‑1 (MQ=255)
                                                                                                                          tGGAGACAGCACTAGTCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                 >  1:3102594/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                 >  1:2865994/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                 >  2:2669155/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                 >  2:119523/1‑140 (MQ=255)
                                                                                                                          tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                 >  1:2177945/1‑140 (MQ=255)
                                                                                                                            gacacacCACAACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTAAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAt               <  2:18100/137‑1 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGACGAGGGCAGGCTTCAGGCGATTCTGGAGGGGAt          >  2:1773033/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt          >  1:662314/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt          >  2:1925926/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt          >  1:965775/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt          >  1:990477/1‑140 (MQ=255)
                                                                                                                                 aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt          >  1:1231338/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                      >  1:1353333/1‑46 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGGGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:63090/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  1:2660540/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:3012720/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:73986/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:1285113/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  1:444235/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:2352320/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  1:445756/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  1:2540741/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg        >  2:234450/1‑140 (MQ=255)
                                                                                                                                   cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGCCTTCAGGCGATTCTTGATGTGATTg        >  2:3011893/1‑140 (MQ=255)
                                                                                                                                        aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg   >  2:2956913/1‑140 (MQ=255)
                                                                                                                                        aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg   >  2:739890/1‑140 (MQ=255)
                                                                                                                                        aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg   >  2:1233498/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                        
CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGG  >  NZ_CP034943/755392‑755667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: