BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	813,492	C→G	5.3%	I52M (ATC→ATG) ‡	nosA →	nitric oxide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	813,492	0	C	G	5.3%	366.2 / 9.5	114	I52M (ATC→ATG) ‡	nosA	nitric oxide synthase
Reads supporting (aligned to +/- strand): ref base C (58/50); new base G (3/3); total (61/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 114 total aligned reads displayed.

TGGAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATC  >  NZ_CP034943/813358‑813622

tGGAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAATAAATCGAgaa                                                                                                                               >  2:1017683/1‑138 (MQ=255)

tGGAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCa                                                                                                                               <  1:2810934/140‑1 (MQ=255)

tGGAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATGGACCGGACCGGAAGCTCTGTACATCCGAAGGAAGCAATCGAGCa                                                                                                                               >  2:2399381/1‑140 (MQ=255)

 ggAACGAAGCGAAAGGTTTTATTGCCGAATGCTATCAGGAATTGGGATAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGTGCAc                                                                                                                              <  2:886686/140‑1 (MQ=255)

  gAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACg                                                                                                                             >  1:2002429/1‑140 (MQ=255)

  gAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACg                                                                                                                             >  1:2211519/1‑140 (MQ=255)

     cGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACgggg                                                                                                                          >  1:174764/1‑140 (MQ=255)

     cGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACgggg                                                                                                                          >  1:1321506/1‑140 (MQ=255)

       aaGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCg                                                                                                                        >  1:245661/1‑140 (MQ=255)

        aGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGa                                                                                                                       >  2:2913933/1‑140 (MQ=255)

        aGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGa                                                                                                                       >  2:1483908/1‑140 (MQ=255)

           gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                    <  1:2598565/140‑1 (MQ=255)

           gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                    <  1:1149183/140‑1 (MQ=255)

           gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                    <  1:1914111/140‑1 (MQ=255)

           gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAACACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                    <  1:1496216/140‑1 (MQ=255)

                cGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCt                                                                                                               <  1:190779/140‑1 (MQ=255)

                     agtggcGAAGGCTATCAGGAATTGGGACAGGAGGAGGAGGTGAAAGCCCGTCTCGATAGCATTAACAGTGAAAGTGACCGGCCCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGgag                                                                                                          <  2:905418/135‑1 (MQ=255)

                     aTTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAACTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGgag                                                                                                          <  2:804550/140‑1 (MQ=255)

                      ttGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGgaga                                                                                                         <  1:2344622/140‑1 (MQ=255)

                            aaTGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGc                                                                                                   <  1:2805813/140‑1 (MQ=255)

                                  aTCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGc                                                                                             <  1:2988392/140‑1 (MQ=255)

                                   tCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCt                                                                                            >  1:1027943/1‑140 (MQ=255)

                                   tCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCt                                                                                            >  2:695266/1‑140 (MQ=255)

                                   tCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCt                                                                                            >  1:2159448/1‑140 (MQ=255)

                                          ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCa                                                                                         >  1:1589484/1‑136 (MQ=255)

                                          ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCa                                                                                         <  2:1589484/136‑1 (MQ=255)

                                          ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACAGGACCGGAAGCTATGTACATACGAAGGAAGAACTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCgg                                                                                     >  2:2515347/1‑140 (MQ=255)

                                             ggAAAGGAGGAGGAAGTGAAAGACCGTCTCGCGGACATTAAAAGTGAAATTGACCTGACCGGAAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAg                                                                                  >  2:2226204/1‑140 (MQ=255)

                                             ggAAAGGAGGAGGAAGTGAAAGACCGTCTCGCGGACATTAAAAGTGAAATTGACCTGACCGGAAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAg                                                                                  >  2:2544763/1‑140 (MQ=255)

                                              gAAAGGAGGAGGAGGTGAACGACCGTCTCGATAGCATTAAAAGTGAACTTGACCGGACCGGAAGCGATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                 <  2:2900608/140‑1 (MQ=255)

                                              gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                 <  2:1321506/140‑1 (MQ=255)

                                              gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                 <  2:636775/140‑1 (MQ=255)

                                              gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                 <  2:174764/140‑1 (MQ=255)

                                              gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                 <  1:1499236/140‑1 (MQ=255)

                                                         gaggTGAAATACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATCGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                      >  1:2124817/1‑140 (MQ=255)

                                                         gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                      <  2:2833662/140‑1 (MQ=255)

                                                         gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                      <  1:964951/140‑1 (MQ=255)

                                                         gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                      <  1:964955/140‑1 (MQ=255)

                                                         gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                      <  2:2940886/140‑1 (MQ=255)

                                                             tGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCg                                                                  >  1:2986310/1‑140 (MQ=255)

                                                                 aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                              <  1:2641223/140‑1 (MQ=255)

                                                                 aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                              <  1:785896/140‑1 (MQ=255)

                                                                 aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                              <  1:1318809/140‑1 (MQ=255)

                                                                 aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                              <  1:2149114/140‑1 (MQ=255)

                                                                   aCCGTCTCGCTAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAACATGGCTGGGAGAAACAGCAACCGCTGCCTCGGCAGATTGTTTTGGAATTCGCTGAAt                                                            >  2:3029808/1‑140 (MQ=255)

                                                                   aCCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGAGTGTTTTGGAATTCGCTGAAt                                                            >  2:3029589/1‑140 (MQ=255)

                                                                           cagaGCATTAAAAGGGAAATTCACCGGACCGGAAGCTATGTACATACGAAGGAAGAAAGCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGa                                                    <  1:1613551/137‑1 (MQ=255)

                                                                             tAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACa                                                  <  1:2779977/140‑1 (MQ=255)

                                                                             tAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACa                                                  >  1:2426239/1‑140 (MQ=255)

                                                                                cATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgac                                               >  1:151439/1‑140 (MQ=255)

                                                                                     aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                          >  2:1393898/1‑140 (MQ=255)

                                                                                     aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGAGTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                          >  2:2188860/1‑140 (MQ=255)

                                                                                     aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCGTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                          >  2:604062/1‑140 (MQ=255)

                                                                                             aTTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGAc                                  <  2:2002429/140‑1 (MQ=255)

                                                                                             aTTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGAc                                  <  2:2211519/140‑1 (MQ=255)

                                                                                                     gaccggaAGCTATGTACATACGAAGGAAGAACTCGCGCCCGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGGGACGTCCGGACGAaggagg                          <  1:1236911/140‑1 (MQ=255)

                                                                                                       ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                        <  1:2226204/140‑2 (MQ=255)

                                                                                                       ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                        <  1:2197897/140‑2 (MQ=255)

                                                                                                       ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                        <  1:2544763/140‑2 (MQ=255)

                                                                                                          gaAGCAATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCTTCGACAGACGTGACGTCCGGACGAAGGAGGAtgtg                     <  2:2893880/140‑1 (MQ=255)

                                                                                                            aGCTATGTACAGACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCg                   <  2:1222393/140‑1 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACCGCAACCGCTGCATCGGCAGCTTGTTTTGGCAGTCGCTGACTGTCATCGACAGACGTGACGGCCGGACGAAGGAGGATGTGCGGGag               >  2:1692034/1‑139 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                              >  2:1151485/1‑125 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                              <  1:1151485/125‑1 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt               >  2:317297/1‑140 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt               >  2:718030/1‑140 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt               >  1:176974/1‑140 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt               >  1:3002176/1‑140 (MQ=255)

                                                                                                                aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGAGTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt               >  1:2773376/1‑140 (MQ=255)

                                                                                                                 tGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATg              >  2:27028/1‑140 (MQ=255)

                                                                                                                 tGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATg              >  2:2093346/1‑140 (MQ=255)

                                                                                                                  gTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGc             <  1:2088696/140‑1 (MQ=255)

                                                                                                                  gTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGc             <  1:2763832/140‑1 (MQ=255)

                                                                                                                   tACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGcc            >  2:2941822/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCAt                                                                                        >  1:2988969/1‑59 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCAt                                                                                        <  2:2988969/59‑1 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  1:1488408/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  1:2522459/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  1:2522095/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  1:2185581/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  1:3009188/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  2:1015287/1‑140 (MQ=255)

                                                                                                                        aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt       >  2:1039601/1‑140 (MQ=255)

                                                                                                                         ccaaggaacCAAGCGAGCACGCGGCGCACAGGGCTTGGAGAAACACCAACCGCTGCCTCCGCAGAGTGGTGCGGAATTCGCTGACTGTCATCGACAGACGTGACGTCCGGACGAAGGAGGCTGTGCGGGATGCCCACttt      <  2:2317068/138‑1 (MQ=255)

                                                                                                                         cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt      >  1:362294/1‑140 (MQ=255)

                                                                                                                         cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt      >  1:2077545/1‑140 (MQ=255)

                                                                                                                         cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt      >  1:1126284/1‑140 (MQ=255)

                                                                                                                           aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAaggagg                          <  2:1338498/118‑1 (MQ=255)

                                                                                                                           aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAaggagg                          >  1:1338498/1‑118 (MQ=255)

                                                                                                                           aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca    >  2:3063427/1‑140 (MQ=255)

                                                                                                                           aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca    >  2:526095/1‑140 (MQ=255)

                                                                                                                           aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca    >  2:88686/1‑140 (MQ=255)

                                                                                                                            aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat   <  1:3004730/140‑1 (MQ=255)

                                                                                                                            aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat   <  1:2913933/140‑1 (MQ=255)

                                                                                                                            aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat   <  1:2749919/140‑1 (MQ=255)

                                                                                                                            aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat   <  1:1483908/140‑1 (MQ=255)

                                                                                                                             ggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTTGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcatc  >  2:1447791/1‑140 (MQ=255)

                                                                                                                             ggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGAAACCGCTGCATCGGCAGATTTGTTTGGAATTCGCTGAATGTAATCGACAGACGTGACGTCAGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcatc  >  2:2040546/1‑140 (MQ=255)

                                                                                                                                    aTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                              <  1:771456/105‑1 (MQ=255)

                                                                                                                                    aTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                              >  2:771456/1‑105 (MQ=255)

TGGAACGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATC  >  NZ_CP034943/813358‑813622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`