Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 266,830 T→A 7.3% I45I (ATT→ATA kdgD → 5‑dehydro‑4‑deoxyglucarate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943266,8300TA 7.3% 641.2 / 32.3 193I45I (ATT→ATAkdgD5‑dehydro‑4‑deoxyglucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (94/82);  new base A (7/7);  total (103/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

Only 100 of 197 total aligned reads displayed.

TGATGAGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACT  >  NZ_CP034943/266694‑266967
                                                                                                                                        |                                                                                                                                         
tgatgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGTAGGCTTGGAAGCGAttttt                                                                                                                                        >  2:2152399/1‑140 (MQ=255)
tgatgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGAttttt                                                                                                                                        >  2:3033796/1‑140 (MQ=255)
  atgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTAt                                                                                                                                      >  1:2434714/1‑140 (MQ=255)
  atgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTAt                                                                                                                                      >  1:2774237/1‑140 (MQ=255)
  atgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTAt                                                                                                                                      <  1:1502898/140‑1 (MQ=255)
  atgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTAt                                                                                                                                      >  1:1027872/1‑140 (MQ=255)
   tgaGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATc                                                                                                                                     <  2:692209/140‑1 (MQ=255)
    gaGCCGTATGAGAAAAGCACCAGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCTAGTTTTTGGGGGAAGAAGGCTTCGAAGCGATTTTTATCg                                                                                                                                    >  2:2422694/1‑140 (MQ=255)
       ccGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCtt                                                                                                                                 >  1:87343/1‑140 (MQ=255)
        cGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGGTGCACCTTTTAACTCACAAGTAAAACTGGCGGAAGAAGCTCTTTTTCAAAATCTCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTtg                                                                                                                                >  2:205659/1‑140 (MQ=255)
          tATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTtgtg                                                                                                                              >  2:928418/1‑140 (MQ=255)
          tATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTtgtg                                                                                                                              >  2:2720856/1‑140 (MQ=255)
              agaAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTc                                                                                                                          <  1:166437/140‑1 (MQ=255)
                  aaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGt                                                                                                                      >  2:2665848/1‑140 (MQ=255)
                    gCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGa                                                                                                                    >  1:60340/1‑140 (MQ=255)
                        ccGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTtg                                                                                                                                <  2:1500792/124‑1 (MQ=255)
                          gCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCa                                                                                                              >  2:805667/1‑140 (MQ=255)
                                aTCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAAtctct                                                                                                        <  1:672158/140‑1 (MQ=255)
                                      ggTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCa                                                                                                  >  2:3018202/1‑140 (MQ=255)
                                           tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                             >  2:1230601/1‑138 (MQ=255)
                                           tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                             >  2:159442/1‑138 (MQ=255)
                                           tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                             >  2:489429/1‑138 (MQ=255)
                                           tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaag                                                                                             >  2:144456/1‑140 (MQ=255)
                                           tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaag                                                                                             >  2:2135580/1‑140 (MQ=255)
                                           tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                >  1:2868433/1‑137 (MQ=255)
                                           tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                <  2:2868433/137‑1 (MQ=255)
                                           tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                <  2:2815567/137‑1 (MQ=255)
                                                 tGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATa                                                                                         >  1:1549002/1‑138 (MQ=255)
                                                     ccTTTTAACTCACAAGGAACACTGGAGGAAGCAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTTGAAGCGATTTTTATCGCTTGTGTGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCa                                                                                   <  1:1619081/140‑1 (MQ=255)
                                                        tttAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAAt                                                                                >  1:2560555/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGTAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  2:1565189/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGACTACGAGCAAATGGTGGAAg                                                                        >  1:2926121/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  1:891750/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  2:2646031/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  1:1493489/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  2:240483/1‑140 (MQ=255)
                                                                acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGACAGAAAGAATACGAGCAAATGGTGGAAg                                                                        >  1:1870285/1‑140 (MQ=255)
                                                                  aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                 >  1:2828769/1‑129 (MQ=255)
                                                                  aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                 >  1:2333430/1‑129 (MQ=255)
                                                                  aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                      >  1:1354903/1‑140 (MQ=255)
                                                                  aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                      <  2:477498/140‑1 (MQ=255)
                                                                  aaGGAAAAATTGATTATGTAGCTCTTTTTAAAAATATCGAGTTTTTGGTGGAATAAGTATTGTAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAATAATACGAGCAAATGTTGGAAGTc                                                                      <  2:2140593/140‑1 (MQ=255)
                                                                            ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                            >  1:368989/1‑140 (MQ=255)
                                                                            ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTGGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                            >  1:1328902/1‑140 (MQ=255)
                                                                               ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCTGCCAGAAAGAATACGAGCAAATGGTGGAAGTAGCACTCTCGGCAg                                                         >  2:2340399/1‑140 (MQ=255)
                                                                               ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                         >  2:239861/1‑140 (MQ=255)
                                                                               ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                         >  1:1989628/1‑140 (MQ=255)
                                                                               ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGAGTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                         >  2:2345765/1‑140 (MQ=255)
                                                                               ggaAGAAGCTCTTTTTAAAAATATCGAGTTTGTGGTGTAAGAAGTCTTGGAAGAGATTTTTATCGCTTGTGGTTCTGGTGAATTTCAATCTCTCATCAAGAAAGAATAAGAGCAAATCGTGGAAGTCTCAATATCGGAAg                                                         >  2:1151342/1‑140 (MQ=255)
                                                                                gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                        >  2:1208887/1‑140 (MQ=255)
                                                                                gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                        >  2:2490601/1‑140 (MQ=255)
                                                                                gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                        >  2:2011957/1‑140 (MQ=255)
                                                                                gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                        >  1:329375/1‑140 (MQ=255)
                                                                                   gaagCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCgg                                                     >  1:503408/1‑140 (MQ=255)
                                                                                       ctctTTTTCAAAATCTCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCggagga                                                 >  1:707622/1‑140 (MQ=255)
                                                                                       ctctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCggagga                                                 >  2:417215/1‑140 (MQ=255)
                                                                                        tctTTTTCAAAATATCGTGTTTTTTGTTGAAGTATGCTTTGAAGCTATTTTTATCGCTTGTTGTTATGGTGAATTTCAATCTCTCAGGCAGAAAGAATACTAGCAAATGGTGGTAGTAGCAATCTCGGCAGCCGTTGGaa                                                <  2:1232784/140‑1 (MQ=255)
                                                                                        tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                <  1:3018202/140‑1 (MQ=255)
                                                                                        tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                <  2:855500/140‑1 (MQ=255)
                                                                                          tttttCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAg                                              >  2:1706522/1‑140 (MQ=255)
                                                                                          tttttCAAAATATCGAGTTTTGGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAg                                              >  2:2326173/1‑140 (MQ=255)
                                                                                              tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                          <  2:826888/140‑1 (MQ=255)
                                                                                              tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                          <  2:2002984/140‑1 (MQ=255)
                                                                                                aaaaTATCTAGTTTTTGGTTGAAGAATGATTGTAAGCGATTTTTATCGATTGTTGGTCTGGTGAATTTAAATCTCTCTTCAAGATATAATACGAGCAATTGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCg                                        <  2:1027872/140‑1 (MQ=255)
                                                                                                aaaaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTTAATTTCAATCTCTCAGCCATAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCg                                        <  2:2434714/140‑1 (MQ=255)
                                                                                                  aaTCTCGAGTTTTTGGTGGAAGAAGGCTTGGAAGAGATTTTTATCTCTTGTGGGTCTGTTTAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGCTTCCGGt                                      <  1:205764/140‑1 (MQ=255)
                                                                                                  aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                      <  2:503408/140‑1 (MQ=255)
                                                                                                  aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                      <  1:2702875/140‑1 (MQ=255)
                                                                                                  aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                      <  1:1240321/140‑1 (MQ=255)
                                                                                                      tCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCACATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTac                                  >  2:723396/1‑140 (MQ=255)
                                                                                                      tCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTac                                  >  2:308736/1‑140 (MQ=255)
                                                                                                       ccagatgttGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                 <  2:295893/133‑1 (MQ=255)
                                                                                                       cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                 <  2:1989628/140‑1 (MQ=255)
                                                                                                       cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                 <  1:3045425/140‑1 (MQ=255)
                                                                                                                    gaagaaGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCACATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcgg                    >  2:131201/1‑140 (MQ=255)
                                                                                                                     cagaagGCTTGGAAGCGATTATTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  2:1821656/139‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:47438/140‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:1610417/140‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:805667/140‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:2132067/140‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:1937247/140‑1 (MQ=255)
                                                                                                                     aagaagGCTTGGAAGAGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:1203821/140‑1 (MQ=255)
                                                                                                                     aagaagGCGTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATGTCACTCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                   <  1:2140972/140‑1 (MQ=255)
                                                                                                                        aagGCTTGGAAGCTATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAAc                <  1:1697167/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt              <  1:239446/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt              <  1:2418420/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt              <  1:2665848/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt              <  1:2997327/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt              <  1:2419981/140‑1 (MQ=255)
                                                                                                                          ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt              <  1:1230601/140‑1 (MQ=255)
                                                                                                                               ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa         <  1:1410098/140‑1 (MQ=255)
                                                                                                                               ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa         <  1:417215/140‑1 (MQ=255)
                                                                                                                               ggAAGCGATGTTTATCGCTTGTGGGTCTGGAGAATTTCAATCTCTCACCCTGAAAGAATACGAGCAGATGGTGGAAGTCGCAATCTCGGCAGCCGGCGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa         <  2:2739906/140‑1 (MQ=255)
                                                                                                                                 aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa       <  1:928418/140‑1 (MQ=255)
                                                                                                                                 aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa       <  1:73922/140‑1 (MQ=255)
                                                                                                                                 aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa       <  1:251172/140‑1 (MQ=255)
                                                                                                                                    cGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCa    >  2:2670529/1‑140 (MQ=255)
                                                                                                                                      aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                        >  2:1618773/1‑86 (MQ=255)
                                                                                                                                      aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt  >  1:1033954/1‑140 (MQ=255)
                                                                                                                                      aTTTTTATCGCTTGAGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt  >  1:2710384/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TGATGAGCCGTATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACT  >  NZ_CP034943/266694‑266967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: