BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	813,501	G→A	5.5%	G55G (GGG→GGA)	nosA →	nitric oxide synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	813,501	0	G	A	5.5%	353.0 / 7.6	108	G55G (GGG→GGA)	nosA	nitric oxide synthase
Reads supporting (aligned to +/- strand): ref base G (54/48); new base A (3/3); total (57/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

Only 100 of 114 total aligned reads displayed.

CGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAACCGCCACCA  >  NZ_CP034943/813363‑813640

cGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACgggg                                                                                                                                            >  1:1321506/1‑140 (MQ=255)

cGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACgggg                                                                                                                                            >  1:174764/1‑140 (MQ=255)

  aaGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCg                                                                                                                                          >  1:245661/1‑140 (MQ=255)

   aGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGACGAAATCGAGCACGGGGCGa                                                                                                                                         >  2:3004730/1‑140 (MQ=255)

   aGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGa                                                                                                                                         >  2:1483908/1‑140 (MQ=255)

   aGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGa                                                                                                                                         >  2:2913933/1‑140 (MQ=255)

      gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                                      <  1:2598565/140‑1 (MQ=255)

      gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                                      <  1:1149183/140‑1 (MQ=255)

      gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                                      <  1:1914111/140‑1 (MQ=255)

      gAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAACACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGaaaa                                                                                                                                      <  1:1496216/140‑1 (MQ=255)

           cGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCt                                                                                                                                 <  1:190779/140‑1 (MQ=255)

                agtggcGAAGGCTATCAGGAATTGGGACAGGAGGAGGAGGTGAAAGCCCGTCTCGATAGCATTAACAGTGAAAGTGACCGGCCCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGgag                                                                                                                            <  2:905418/135‑1 (MQ=255)

                aTTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAACTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGgag                                                                                                                            <  2:804550/140‑1 (MQ=255)

                       aaTGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGc                                                                                                                     <  1:2805813/140‑1 (MQ=255)

                             aTCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGc                                                                                                               <  1:2988392/140‑1 (MQ=255)

                              tCAGGAATTGGGAAAGGAGGAGGAGGTGACAGACCGTCTCGATAGCATTAAATGTGCAATTGACCGGACCGGAAGCTATGTACCTACGACGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCACCCGCt                                                                                                              >  1:2122950/1‑140 (MQ=255)

                              tCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCt                                                                                                              >  1:1027943/1‑140 (MQ=255)

                              tCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCt                                                                                                              >  1:2159448/1‑140 (MQ=255)

                                     ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCa                                                                                                           <  2:1589484/136‑1 (MQ=255)

                                     ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCa                                                                                                           >  1:1589484/1‑136 (MQ=255)

                                     ttGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACAGGACCGGAAGCTATGTACATACGAAGGAAGAACTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCgg                                                                                                       >  2:2515347/1‑140 (MQ=255)

                                        ggAAAGGAGGAGGAAGTGAAAGACCGTCTCGCGGACATTAAAAGTGAAATTGACCTGACCGGAAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAg                                                                                                    >  2:2197897/1‑140 (MQ=255)

                                        ggAAAGGAGGAGGAAGTGAAAGACCGTCTCGCGGACATTAAAAGTGAAATTGACCTGACCGGAAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAg                                                                                                    >  2:2544763/1‑140 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAACGACCGTCTCGATAGCATTAAAAGTGAACTTGACCGGACCGGAAGCGATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  2:2900608/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  1:1620083/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  2:2735008/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  2:1321506/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  2:636775/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  2:174764/140‑1 (MQ=255)

                                         gAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGa                                                                                                   <  1:1499236/140‑1 (MQ=255)

                                                    gaggTGAAATACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATCGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                                        >  1:2124817/1‑140 (MQ=255)

                                                    gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                                        <  2:2940886/140‑1 (MQ=255)

                                                    gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                                        <  1:964951/140‑1 (MQ=255)

                                                    gaggTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGaa                                                                                        <  1:964955/140‑1 (MQ=255)

                                                            aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                                                <  1:785896/140‑1 (MQ=255)

                                                            aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                                                <  1:1318809/140‑1 (MQ=255)

                                                            aGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGa                                                                                <  1:2149114/140‑1 (MQ=255)

                                                              aCCGTCTCGCTAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAACATGGCTGGGAGAAACAGCAACCGCTGCCTCGGCAGATTGTTTTGGAATTCGCTGAAt                                                                              >  2:3029808/1‑140 (MQ=255)

                                                                  tctcGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCa                                                                          >  1:2871579/1‑140 (MQ=255)

                                                                        tAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACa                                                                    <  1:2779977/140‑1 (MQ=255)

                                                                        tAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACa                                                                    >  1:2426239/1‑140 (MQ=255)

                                                                           cATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgac                                                                 >  1:151439/1‑140 (MQ=255)

                                                                                aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                                            >  2:1393898/1‑140 (MQ=255)

                                                                                aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGAGTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                                            >  2:2188860/1‑140 (MQ=255)

                                                                                aaaGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCGTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAgacgtgac                                                            >  2:604062/1‑140 (MQ=255)

                                                                                      aaaTTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCgg                                                      >  2:1130472/1‑140 (MQ=255)

                                                                                        aTTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGAc                                                    <  2:2211519/140‑1 (MQ=255)

                                                                                        aTTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGAc                                                    <  2:2002429/140‑1 (MQ=255)

                                                                                                gaccggaAGCTATGTACATACGAAGGAAGAACTCGCGCCCGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGGGACGTCCGGACGAaggagg                                            <  1:1236911/140‑1 (MQ=255)

                                                                                                  ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                                          <  1:2197897/140‑2 (MQ=255)

                                                                                                  ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                                          <  1:2226204/140‑2 (MQ=255)

                                                                                                  ccggaAGCTATGTACATACGAAGGAAGAGCTGGAGCACGGAGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTCTGGAATTCGCTGAATGTTATCGACAGACGAGACGTCCGGACGAAggaggaa                                          <  1:2544763/140‑2 (MQ=255)

                                                                                                     gaAGCAATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCTTCGACAGACGTGACGTCCGGACGAAGGAGGAtgtg                                       <  2:2893880/140‑1 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                                                >  2:1151485/1‑125 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                                                <  1:1151485/125‑1 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  2:718030/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  1:176974/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  2:317297/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  1:3002176/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  2:1552515/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGAGCGGGAt                                 >  2:692654/1‑140 (MQ=255)

                                                                                                           aTGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGAGTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGAt                                 >  1:2773376/1‑140 (MQ=255)

                                                                                                            tGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATg                                >  2:27028/1‑140 (MQ=255)

                                                                                                            tGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATg                                >  2:2093346/1‑140 (MQ=255)

                                                                                                             gtccATACGAACGAAGAACTCGAGCACGGGGCGACAATGGCTTGGAGAAACAGCAACCCCTGCATCGGCAGATTGTTTTGGAATTCGCTGACTGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGc                               <  1:1460132/137‑1 (MQ=255)

                                                                                                             gTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGc                               <  1:2763832/140‑1 (MQ=255)

                                                                                                             gTACAGACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGCATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGc                               <  1:2600435/140‑1 (MQ=255)

                                                                                                              tACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGcc                              >  2:2941822/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCAt                                                                                                          <  2:2988969/59‑1 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCAt                                                                                                          >  1:2988969/1‑59 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  1:2522459/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  1:2522095/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  1:3009188/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  1:1488408/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  2:1015287/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  2:1039601/1‑140 (MQ=255)

                                                                                                                   aCGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCtt                         >  1:2185581/1‑140 (MQ=255)

                                                                                                                    ccaaggaacCAAGCGAGCACGCGGCGCACAGGGCTTGGAGAAACACCAACCGCTGCCTCCGCAGAGTGGTGCGGAATTCGCTGACTGTCATCGACAGACGTGACGTCCGGACGAAGGAGGCTGTGCGGGATGCCCACttt                        <  2:2317068/138‑1 (MQ=255)

                                                                                                                    cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt                        >  1:1126284/1‑140 (MQ=255)

                                                                                                                    cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt                        >  1:362294/1‑140 (MQ=255)

                                                                                                                    cGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCttt                        >  1:2077545/1‑140 (MQ=255)

                                                                                                                      aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAaggagg                                            <  2:1338498/118‑1 (MQ=255)

                                                                                                                      aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca                      >  2:88686/1‑140 (MQ=255)

                                                                                                                      aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca                      >  2:3063427/1‑140 (MQ=255)

                                                                                                                      aaggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtca                      >  2:526095/1‑140 (MQ=255)

                                                                                                                       aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat                     <  1:329658/140‑1 (MQ=255)

                                                                                                                       aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat                     <  1:3004730/140‑1 (MQ=255)

                                                                                                                       aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat                     <  1:2913933/140‑1 (MQ=255)

                                                                                                                       aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat                     <  1:2749919/140‑1 (MQ=255)

                                                                                                                       aggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcat                     <  1:1483908/140‑1 (MQ=255)

                                                                                                                        ggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTTGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcatc                    >  2:1447791/1‑140 (MQ=255)

                                                                                                                        ggaagAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGAAACCGCTGCATCGGCAGATTTGTTTGGAATTCGCTGAATGTAATCGACAGACGTGACGTCAGGACGAAGGAGGATGTGCGGGATGCCCTCTTtcatc                    >  2:2040546/1‑140 (MQ=255)

                                                                                                                               aTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                                                <  1:771456/105‑1 (MQ=255)

                                                                                                                               aTCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAag                                                >  2:771456/1‑105 (MQ=255)

                                                                                                                                   aGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGACGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAAcc         >  2:2706460/1‑140 (MQ=255)

                                                                                                                                    gCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTTCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAACCg        <  2:273557/140‑1 (MQ=255)

                                                                                                                                    gCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAACCg        >  1:1256715/1‑140 (MQ=255)

                                                                                                                                        ggggCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAACCGccac    >  1:1754734/1‑140 (MQ=255)

                                                                                                                                          ggCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGAc                                                    <  2:733796/90‑1 (MQ=255)

                                                                                                                                          ggCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTAATCACATTGAAACCGccacca  >  2:835923/1‑140 (MQ=255)

CGAAGCGAAAGCGTTTATTGCCGAATGCTATCAGGAATTGGGAAAGGAGGAGGAGGTGAAAGACCGTCTCGATAGCATTAAAAGTGAAATTGACCGGACCGGAAGCTATGTACATACGAAGGAAGAAATCGAGCACGGGGCGAAAATGGCTTGGAGAAACAGCAACCGCTGCATCGGCAGATTGTTTTGGAATTCGCTGAATGTCATCGACAGACGTGACGTCCGGACGAAGGAGGATGTGCGGGATGCCCTCTTTCATCACATTGAAACCGCCACCA  >  NZ_CP034943/813363‑813640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`