Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 847,255 T→A 6.0% D277V (GAT→GTT)  EO946_RS04305 ← NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943847,2550TA 6.0% 551.5 / 5.2 176D277V (GAT→GTT) EO946_RS04305NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (63/97);  new base A (11/2);  total (75/101)
Fisher's exact test for biased strand distribution p-value = 2.28e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACATTTTGAAACC  >  NZ_CP034943/847131‑847394
                                                                                                                            |                                                                                                                                           
ttAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTGCTTAAAAGGTGAGGCGGTGTAATTTTTTTATTAAATTTTTAAATTCGGGCCCAACCAAGTCTACgcacc                                                                                                                              >  1:2763478/1‑136 (MQ=255)
ttAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCGCACCATAAACGGAGAGGCTCGGTAAATATTTTTTTAAATTTATAAGTGCGGCCCACAACAATTATCCGaaac                                                                                                                              >  1:608456/1‑138 (MQ=255)
        cTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTACTTAAACGGAGAGGGTCTCTAATTATTTTATACAATTTTTAATTGCGGCCCAATCCAAGTCTCCGAAGCCtttttaa                                                                                                                      >  2:585071/1‑138 (MQ=255)
          tgatgaTTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTGTCTAATTATTTTATACATTTTATAATTTCGGCCCAATCCAATTCTCCGAAGCCTTTTTCAAt                                                                                                                    >  2:3071789/1‑140 (MQ=255)
          tgatgaTTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAAAGGAGAGGCTCTCTAATTATTTTATAAATTTTATAAGTTCGGCCCAAACCAAGTCTACGAAGCCTTTTTAAAt                                                                                                                    >  2:1962227/1‑140 (MQ=255)
          tgatgaTTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCACGACTTAAACGGGCCGGCTATCTAAATAATTTAAACAATTTTTAAATGGGGCCCCATACAAATCTCAGAAACCTTTTTAAAt                                                                                                                    >  2:1043927/1‑140 (MQ=255)
                tttAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCt                                                                                                              <  1:530758/140‑1 (MQ=255)
                tttAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCt                                                                                                              <  1:1780961/140‑1 (MQ=255)
                 ttAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTACTTAAAAGGAGATGCGCTATAATTATTTTTTAAATTTTATAATTTCGGGCCAAACCAAGTATACCAAACCTTTTTAAATTACGCtt                                                                                                             >  1:242220/1‑140 (MQ=255)
                  tAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCttt                                                                                                            >  2:754066/1‑140 (MQ=255)
                  tAATGTTTGAGAAAGAAACGCACCCGCCGTCTTTGTACCGAGAAGAGCCTCTCCATAAACGGAGAGGCACTCTAATTATTATATACATGTAATAAGTGCGGCCCAAGCCCAGTCTCCGAAGCCTTTTTCAATTCCGCttt                                                                                                            <  1:504681/140‑1 (MQ=255)
                        ttGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAt                                                                                                      <  1:1317484/140‑1 (MQ=255)
                        ttGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAt                                                                                                      <  1:135809/140‑1 (MQ=255)
                        ttGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAAt                                                                                                      <  2:1513622/140‑1 (MQ=255)
                         tGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATg                                                                                                     <  1:1468569/140‑1 (MQ=255)
                         tGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCACTCCATAAAACGAGAGGGGCGCTAATTATTTTATAAAATTTATAAGTGCGGCCCAAACCAACTCTACGAAACCATTTTGAATTACCCGTTAAAAAta                                                                                                     >  1:12077/1‑139 (MQ=255)
                               agaaaTGAAGCTGCCGTCTTTGTAACGAGAAGAGGCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATAAATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTAAAAATGGCCCtt                                                                                               >  2:1875139/1‑140 (MQ=255)
                               agaaaTGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTGCTTAAAAGGAGAGGCGCTCTAATTATTTTATACATTTTATAAGGGCGGCCCAAACCAAGTCTCCGAACCCTTTTTCCAATCCGCATTAAAAAAGTcccat                                                                                               >  2:2375521/1‑138 (MQ=255)
                               agaaaTGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACAATTTATAAGTGCGGCCCAATCCAAGTCTCCCAAACCTTTTTCAATTACGCATTCAAAAttggccat                                                                                               >  2:1429279/1‑133 (MQ=255)
                                          tGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCgg                                                                                    <  2:881809/140‑1 (MQ=255)
                                          ggCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCgg                                                                                    <  2:2121580/139‑1 (MQ=255)
                                           gCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGc                                                                                   <  1:2674836/140‑1 (MQ=255)
                                           gCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACCGCGAGGCTGGCTAAATATTTTATACAATTTTTAATTGCGGCCCAAACCAAGTCTCCGAAACCTTTTTCAATTACGCTTTCAAAATGGCCCGTGGGCACCGCGGc                                                                                   >  1:2996521/1‑140 (MQ=255)
                                           gCCGTCTTTGTAACGAGAAGAGCCTATCCGTGAAAGGAGAGGCTCTGGAAATATTTTGTAGAATTTAAAAGTTCGGCCCAAGCCCAGTGTGCCCACCCTTTTTCAATTGCGCTTTCAACAATGCCCTTGGCCAGcgacgc                                                                                   >  1:6437/1‑135 (MQ=255)
                                           gCCGTCTTTGTAACGAGAAGAGCCACTCCTTAAAAGGAGAGGGTGTCGAATTATTTTATACATTTTATAATTTCGGCCCAAACCAAGTATCCGTACACTTTTTAAATTACGCTATAAAAATTGGCCTTTGCCAGCTCGGc                                                                                   >  1:2236097/1‑140 (MQ=255)
                                           gCCGTCTTTGGAACGAGAAGAGCCTCTCCTTACACGGAGAGGCTCTCTCATTATTTTATACATTTTATAAGTGCGGCCCACTCCAACTCTCCGAAGCCTTTTTCAATGCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGc                                                                                   <  1:653855/140‑1 (MQ=255)
                                            ccGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCa                                                                                  <  1:172331/140‑1 (MQ=255)
                                            ccGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCa                                                                                  <  1:818471/140‑1 (MQ=255)
                                            cagTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACACTTTATAAGTGCGGCCCACTCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCa                                                                                  <  1:818332/138‑1 (MQ=255)
                                                 tttGTACCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGagg                                                                             <  2:3009293/140‑1 (MQ=255)
                                                 tttGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCACTCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGagg                                                                             <  2:1331124/140‑1 (MQ=255)
                                                 tttGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGagg                                                                             <  1:404318/140‑1 (MQ=255)
                                                 tttGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCGTTAAAAATGGCCCTTGGCCAGCGCGGCAAGagg                                                                             >  2:2674836/1‑140 (MQ=255)
                                                    gTACCGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:2600664/140‑1 (MQ=255)
                                                    gTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:2829200/140‑1 (MQ=255)
                                                    gTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:409947/140‑1 (MQ=255)
                                                    gTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:1602222/140‑1 (MQ=255)
                                                    gTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:1716769/140‑1 (MQ=255)
                                                    gTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTAGTTTCTACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtag                                                                          <  2:2647309/140‑1 (MQ=255)
                                                      aaagagaAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCCTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGaggtaggt                                                                        <  1:3009742/137‑1 (MQ=255)
                                                         gagaAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTg                                                                     <  1:1081681/140‑1 (MQ=255)
                                                           gaagaGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                                                   <  1:1023639/140‑1 (MQ=255)
                                                           gaagaGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                                                   <  1:2118925/140‑1 (MQ=255)
                                                           gaagaGCCTCTCCTTAAACGGAGAGGCGCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGGGGTAGGTTTGCa                                                                   <  1:2455130/140‑1 (MQ=255)
                                                                gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  2:945832/140‑1 (MQ=255)
                                                                gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  2:2930188/140‑1 (MQ=255)
                                                                gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  2:12077/140‑1 (MQ=255)
                                                                gCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  2:1048402/140‑1 (MQ=255)
                                                                gCCGCTCCTTAACCGGAGAGGCTCTCTAAGTATTTGCTACAGTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  1:2622462/140‑1 (MQ=255)
                                                                cccTCTCCTAAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGc                                                              <  2:3048424/139‑1 (MQ=255)
                                                                   tctcCATAAACGGCGAGGGTCGGTAATTATTTTATACATTTTTTAAGTGCGGCCCAATCCAACTGTCCGAAGCCTTTTTGAATTACCCTTTTAAAATGGGCCGTGGGCACCTAGGGCAGAGGTATGTTTTGAGAGGGCTg                                                           >  2:2031700/1‑140 (MQ=255)
                                                                       cTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       <  2:296798/140‑1 (MQ=255)
                                                                               gagaGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggc                                               <  2:1109815/140‑1 (MQ=255)
                                                                                agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                                              >  1:1625963/1‑140 (MQ=255)
                                                                                agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                                              >  2:3058693/1‑140 (MQ=255)
                                                                                agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                                              >  1:1933791/1‑140 (MQ=255)
                                                                                agagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTgcggcggcg                                              >  1:2036431/1‑140 (MQ=255)
                                                                                 gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGa                                             >  1:2495717/1‑140 (MQ=255)
                                                                                 gagGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCAGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGa                                             >  1:331931/1‑140 (MQ=255)
                                                                                    gCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTg                                          <  2:526691/140‑1 (MQ=255)
                                                                                     ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                                         <  1:2063560/140‑1 (MQ=255)
                                                                                     ctctctAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGc                                         <  1:1011364/140‑1 (MQ=255)
                                                                                              tattTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGt                                <  1:918406/140‑1 (MQ=255)
                                                                                              tattTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGt                                <  1:2383056/140‑1 (MQ=255)
                                                                                              tattGTATCCATGTTATAAGTGCGGCCCAAACCAAGTCGCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCCGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGt                                <  2:1660936/140‑1 (MQ=255)
                                                                                               attTTATACCTTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa                               <  2:691993/140‑1 (MQ=255)
                                                                                               attTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGagg                                                                             >  2:1739827/1‑94 (MQ=255)
                                                                                               attTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCa                                                                   <  2:2209340/104‑1 (MQ=255)
                                                                                               attTTAGACATTTTAGAAGTGCGGCCCCCGCCAAGTCTCCGAACCCTTTTCCACTTCCGCTTTCAAACTGGCCCTTGGCCAGCTCGGCAAGAGGTAGGGGTGCACAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTa                               <  1:186771/140‑1 (MQ=255)
                                                                                                  ttATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATcc                            >  1:1670606/1‑140 (MQ=255)
                                                                                                    ctaCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:159950/139‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCACGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  2:1454536/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:154525/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:2346121/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:43495/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          >  2:43495/1‑140 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:230560/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:1046239/140‑1 (MQ=255)
                                                                                                    ataCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  1:3071789/140‑1 (MQ=255)
                                                                                                    agaccttttATAAGTGCGGCCCACACCACGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCtt                          <  2:777209/135‑1 (MQ=255)
                                                                                                      aCATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCa                        <  2:2544637/140‑1 (MQ=255)
                                                                                                          tttATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGccc                    >  2:2471918/1‑140 (MQ=255)
                                                                                                          tttATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGccc                    >  2:2263944/1‑140 (MQ=255)
                                                                                                          tttATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGccc                    >  1:1728815/1‑140 (MQ=255)
                                                                                                            tataAGTGCGTCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCGGCTTTCAAAATGGGCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTa                  <  2:1792643/140‑1 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCCAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       >  1:2192341/1‑95 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCCAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTCAAGACAtt          >  1:2794337/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       >  1:2540613/1‑95 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       <  2:2540613/95‑1 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       >  1:1949351/1‑95 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCt                                                       <  2:1949351/95‑1 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  1:2904955/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  1:1653809/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  1:2474225/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  2:253920/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  1:2696136/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtt          >  1:2125190/1‑140 (MQ=255)
                                                                                                                    cGGCCCAATCCAAGTCTCCGAAGACTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGAGTGGTATCCTTCAGACCTAAAGACAtt          >  1:2636671/1‑140 (MQ=255)
                                                                                                                      gCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACAtttt        >  2:2238111/1‑140 (MQ=255)
                                                                                                                            tCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACATTTTGAAAcc  >  1:577411/1‑140 (MQ=255)
                                                                                                                            |                                                                                                                                           
TTAAAAGCCTTGATGATTTAATGTTTGAGAAAGAAATGAAGCTGCCGTCTTTGTAACGAGAAGAGCCTCTCCTTAAACGGAGAGGCTCTCTAATTATTTTATACATTTTATAAGTGCGGCCCAATCCAAGTCTCCGAAGCCTTTTTCAATTCCGCTTTCAAAATGGCCCTTGGCCAGCTCGGCAAGAGGTAGGTTTGCAGAGGCCTGTTCTGCGGCGGCGAGTGCCAGATTGGTATCCTTCAGCCCTAAAGACATTTTGAAACC  >  NZ_CP034943/847131‑847394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: