Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 871,761 G→A 5.0% T312T (ACG→ACA EO946_RS04420 → ABC transporter ATP‑binding protein/permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943871,7610GA 5.0% 385.3 / 7.4 120T312T (ACG→ACAEO946_RS04420ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base G (41/72);  new base A (3/3);  total (44/76)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 123 total aligned reads displayed.

CACTCAAGTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGAAA  >  NZ_CP034943/871623‑871900
                                                                                                                                          |                                                                                                                                           
cACTCAAGTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACgg                                                                                                                                            <  1:1598995/140‑1 (MQ=255)
     aaGTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGc                                                                                                                                       >  1:1934714/1‑140 (MQ=255)
      aGTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGGGCGCTTTCGATGTTTCCGTTTTTGCTGATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCg                                                                                                                                      <  1:2435163/140‑1 (MQ=255)
       gTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGa                                                                                                                                     >  1:1112931/1‑140 (MQ=255)
       gTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGa                                                                                                                                     >  2:1612758/1‑140 (MQ=255)
         ctgTGATGTTGTTGCTTTTATTAACTACGAAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATg                                                                                                                                   <  1:2381104/138‑1 (MQ=255)
          ggTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGa                                                                                                                                  >  1:423198/1‑140 (MQ=255)
          ggTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGa                                                                                                                                  >  1:420988/1‑140 (MQ=255)
           gTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAg                                                                                                                                 >  2:2804369/1‑140 (MQ=255)
             gATGTTGTTGCGCTTATTAACTAGGCAACACGAATTACGGGTGCGGTTTCGATGTTTCCGTTTTTGATTAGGATTTTCACTAGGGCGAAGGCTTGCGGTGATAGAATCGGCGCGGTGCTTGAAACGGAAGGCGATGAGCg                                                                                                                               >  2:810427/1‑140 (MQ=255)
                    tttcGATTATTAACTACGAAACACGAATTCCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                        <  1:406059/137‑1 (MQ=255)
                    ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                        <  1:313647/140‑1 (MQ=255)
                    ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                        <  1:1652796/140‑1 (MQ=255)
                    ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGGTTCCGTTTTTGATTATGATTGTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                        <  2:1080934/140‑1 (MQ=255)
                    ttgCGATTATTAACTACGCAACACGAATTACGGGGGCGCTTTCGATGTTTCCGTTTTTGTTTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATATAATCTTAGAGGTTATTTAAACGGAAGGCGATGAGCgggttgaa                                                                                                                        <  2:1978794/140‑6 (MQ=255)
                    ttgCGATTATTAACCACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTGACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                        <  2:334648/140‑1 (MQ=255)
                     tgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAg                                                                                                                       <  1:1805074/140‑1 (MQ=255)
                      gCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAgg                                                                                                                      >  1:2375165/1‑140 (MQ=255)
                          ttattaACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGcatc                                                                                                                  <  2:2198422/140‑1 (MQ=255)
                                    cgccagacGAATTCCGGGTGCGCGTTCGATGTTTCCGTTTTTGATGATGATTTTGACTAGGGCGAAGGCTTCAGGTTATTGAATCGGCGAGGTGCTTGTAAATGAAGGCGATGAGCGGGAGGAAGGGATCATATCTGATc                                                                                                        <  2:312256/134‑1 (MQ=255)
                                    cGCAAGAGGCATTACGGGTGCGGTTTCGTGTTTTCCGTTTTTGCTTATGATTTGCACTAGGGCGAACGGTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGGGATGAGCGGGAGGAAGGCATGATATCTGATc                                                                                                        <  2:1650482/140‑1 (MQ=255)
                                    cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                        >  2:709195/1‑140 (MQ=255)
                                    cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                        >  1:1317089/1‑140 (MQ=255)
                                    cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                        <  2:2325352/140‑1 (MQ=255)
                                    cGCAACACGAATTACCGGCGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACCCGGGCGAAGGCTTCAGGGGATAGAATCGGTGAGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATc                                                                                                        >  2:2196015/1‑140 (MQ=255)
                                    cGCAACACGAATTACAGGCGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACCCGGGCGAAGGCTTCAGGGGATAGAATCGGTGAGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATc                                                                                                        >  2:520159/1‑140 (MQ=255)
                                     gCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCg                                                                                                       <  2:481262/140‑1 (MQ=255)
                                      cAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTCTGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGGAGGCATCATATCTGATCgg                                                                                                      >  2:1603796/1‑139 (MQ=255)
                                      cAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGt                                                                                                      >  1:1519291/1‑140 (MQ=255)
                                           cGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTc                                                                                                 <  2:2216201/140‑1 (MQ=255)
                                              atgaCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                              <  1:662568/137‑1 (MQ=255)
                                              aTTCCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                              <  1:2636610/140‑1 (MQ=255)
                                              aTTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                              <  1:1612758/140‑1 (MQ=255)
                                              aTTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCAATAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                              <  2:1346570/140‑1 (MQ=255)
                                                   gggTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGggagga                                                                                         <  2:742207/140‑1 (MQ=255)
                                                   gggTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGggagga                                                                                         <  2:742266/140‑1 (MQ=255)
                                                      tGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTg                                                                                      >  2:2878383/1‑140 (MQ=255)
                                                      tGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTg                                                                                      >  2:1407051/1‑140 (MQ=255)
                                                       gcgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGa                                                                                     <  2:2259582/140‑1 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  1:1097147/1‑140 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  2:2811909/1‑140 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  1:1016444/1‑140 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  1:1017627/1‑140 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  1:2195555/1‑140 (MQ=255)
                                                        cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                    >  1:1574618/1‑140 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:1516597/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1582091/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:2383183/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:426967/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:281557/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1310014/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1160237/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1137529/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:2749055/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1016444/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:2031822/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:491958/140‑1 (MQ=255)
                                                           tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  1:1992335/140‑1 (MQ=255)
                                                           tttCGATGTTGCCGTGTGTGAGTCTGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGGGCTTGAACCGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:1778499/140‑1 (MQ=255)
                                                           tttCGATGCTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                 <  2:284425/140‑1 (MQ=255)
                                                                  gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                          <  1:2637459/140‑1 (MQ=255)
                                                                  gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                          <  1:1202834/140‑1 (MQ=255)
                                                                  gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                          <  1:1402390/140‑1 (MQ=255)
                                                                     tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                       >  1:222001/1‑140 (MQ=255)
                                                                     tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                       >  1:283462/1‑140 (MQ=255)
                                                                       cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGCTCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                     <  2:1322257/140‑1 (MQ=255)
                                                                       cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                     <  1:402818/140‑1 (MQ=255)
                                                                       cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                     <  2:1316652/140‑1 (MQ=255)
                                                                            ttGTTTATGATATTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  1:2769785/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGTTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  2:106853/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  1:58075/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  2:596104/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  1:218676/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  1:2936143/140‑1 (MQ=255)
                                                                            ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                <  2:1981210/140‑1 (MQ=255)
                                                                                   tGATTTTCACTAGGGCGCAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCt                                                         <  2:1614831/140‑1 (MQ=255)
                                                                                         tCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATg                                                   >  2:1817384/1‑140 (MQ=255)
                                                                                           aCTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGa                                                 <  1:1682701/140‑1 (MQ=255)
                                                                                                           cAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCgg                                 >  1:45491/1‑140 (MQ=255)
                                                                                                            aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                <  1:2804369/140‑1 (MQ=255)
                                                                                                            aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                <  2:2444452/140‑1 (MQ=255)
                                                                                                            aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                <  2:2528970/140‑1 (MQ=255)
                                                                                                            aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                <  2:1302950/140‑1 (MQ=255)
                                                                                                            aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGACGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                <  2:2830062/140‑1 (MQ=255)
                                                                                                                 aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGtc                           >  1:490762/1‑140 (MQ=255)
                                                                                                                 aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGta                           >  2:2259319/1‑139 (MQ=255)
                                                                                                                 aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTGTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACACAGAAGCGCTGCGGAATGtc                           >  2:1073797/1‑140 (MQ=255)
                                                                                                                   aGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGtctc                         >  2:2769950/1‑140 (MQ=255)
                                                                                                                      ctCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCAtt                      <  2:1469726/139‑1 (MQ=255)
                                                                                                                      aTCGGCGAGGTGCCTGAAACGGACGGCGAGGAGCGGGAGGACGGCATCATATCTGATCGTTTGTCGGGGAGGATTGTGTTTCAGCATGTCTCCTTCCGTTATCCTGAAATGGACATAGAAGCGCTGCGGAATGTCTCAtt                      <  2:1471345/140‑1 (MQ=255)
                                                                                                                         ggCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTc                   >  2:474439/1‑140 (MQ=255)
                                                                                                                             aGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCa               <  2:420988/140‑1 (MQ=255)
                                                                                                                             aGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCa               <  2:423198/140‑1 (MQ=255)
                                                                                                                             aGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATCCTGAAATGGACAGAGAAGCGCTCAGGAATGTCTCATTTTCCGCa               <  1:2196015/140‑1 (MQ=255)
                                                                                                                             aGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATCCTGAAATGGACAGAGAAGCGCTCAGGAATGTCTCATTTTCCGCa               <  1:520159/140‑1 (MQ=255)
                                                                                                                               gTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCaaa             >  1:2371641/1‑140 (MQ=255)
                                                                                                                               gTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATc                                                           >  2:3008943/1‑94 (MQ=255)
                                                                                                                               gTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATc                                                           <  1:3008943/94‑1 (MQ=255)
                                                                                                                                   ttGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCg         <  2:2195555/140‑1 (MQ=255)
                                                                                                                                          ggAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTAATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGaaa  >  1:789301/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CACTCAAGTCGGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGAAA  >  NZ_CP034943/871623‑871900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: