Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 871,770 T→C 5.0% D315D (GAT→GAC EO946_RS04420 → ABC transporter ATP‑binding protein/permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943871,7700TC 5.0% 395.1 / 7.4 119D315D (GAT→GACEO946_RS04420ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base T (39/73);  new base C (3/3);  total (42/77)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 119 total aligned reads displayed.

GGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGAAACGATCGCG  >  NZ_CP034943/871633‑871908
                                                                                                                                         |                                                                                                                                          
ggTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGa                                                                                                                                          >  1:420988/1‑140 (MQ=255)
 gTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAg                                                                                                                                         >  2:2804369/1‑140 (MQ=255)
   gATGTTGTTGCGCTTATTAACTAGGCAACACGAATTACGGGTGCGGTTTCGATGTTTCCGTTTTTGATTAGGATTTTCACTAGGGCGAAGGCTTGCGGTGATAGAATCGGCGCGGTGCTTGAAACGGAAGGCGATGAGCg                                                                                                                                       >  2:810427/1‑140 (MQ=255)
          tttcGATTATTAACTACGAAACACGAATTCCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                                <  1:406059/137‑1 (MQ=255)
          ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                                <  1:1652796/140‑1 (MQ=255)
          ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                                <  1:313647/140‑1 (MQ=255)
          ttgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGGTTCCGTTTTTGATTATGATTGTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                                <  2:1080934/140‑1 (MQ=255)
          ttgCGATTATTAACTACGCAACACGAATTACGGGGGCGCTTTCGATGTTTCCGTTTTTGTTTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATATAATCTTAGAGGTTATTTAAACGGAAGGCGATGAGCgggttgaa                                                                                                                                <  2:1978794/140‑6 (MQ=255)
          ttgCGATTATTAACCACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTGACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGaa                                                                                                                                <  2:334648/140‑1 (MQ=255)
           tgCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAg                                                                                                                               <  1:1805074/140‑1 (MQ=255)
           tgCGATTATTAACTAAGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAg                                                                                                                               <  1:179302/140‑1 (MQ=255)
            gCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAgg                                                                                                                              >  1:2375165/1‑140 (MQ=255)
                ttattaACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGcatc                                                                                                                          <  2:2198422/140‑1 (MQ=255)
                          cgccagacGAATTCCGGGTGCGCGTTCGATGTTTCCGTTTTTGATGATGATTTTGACTAGGGCGAAGGCTTCAGGTTATTGAATCGGCGAGGTGCTTGTAAATGAAGGCGATGAGCGGGAGGAAGGGATCATATCTGATc                                                                                                                <  2:312256/134‑1 (MQ=255)
                          cGCAAGAGGCATTACGGGTGCGGTTTCGTGTTTTCCGTTTTTGCTTATGATTTGCACTAGGGCGAACGGTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGGGATGAGCGGGAGGAAGGCATGATATCTGATc                                                                                                                <  2:1650482/140‑1 (MQ=255)
                          cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                                >  1:1317089/1‑140 (MQ=255)
                          cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                                >  2:709195/1‑140 (MQ=255)
                          cGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATc                                                                                                                >  1:2259582/1‑140 (MQ=255)
                          cGCAACACGAATTACAGGCGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACCCGGGCGAAGGCTTCAGGGGATAGAATCGGTGAGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATc                                                                                                                >  2:520159/1‑140 (MQ=255)
                           gCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCg                                                                                                               <  2:481262/140‑1 (MQ=255)
                            cAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTCTGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGGAGGCATCATATCTGATCgg                                                                                                              >  2:1603796/1‑139 (MQ=255)
                            cAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCCGGTGATAGAAACGGCGAGGTTATTGAAACGGAAGGCGATGAGCGGGAGGAAGGCTTCATATCTGATCgg                                                                                                              >  2:122242/1‑139 (MQ=255)
                            cAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGt                                                                                                              >  1:1519291/1‑140 (MQ=255)
                                 cGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTc                                                                                                         <  2:2216201/140‑1 (MQ=255)
                                    atgaCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                                      <  1:662568/137‑1 (MQ=255)
                                    aTTCCGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                                      <  1:2636610/140‑1 (MQ=255)
                                    aTTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                                      <  1:1612758/140‑1 (MQ=255)
                                    aTTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCAATAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCggg                                                                                                      <  2:1346570/140‑1 (MQ=255)
                                         gggTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGggagga                                                                                                 <  2:742207/140‑1 (MQ=255)
                                         gggTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGggagga                                                                                                 <  2:742266/140‑1 (MQ=255)
                                            tGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTg                                                                                              >  2:2878383/1‑140 (MQ=255)
                                            tGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTg                                                                                              >  2:1407051/1‑140 (MQ=255)
                                             gcgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGa                                                                                             <  2:2259582/140‑1 (MQ=255)
                                              cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                            >  1:1017627/1‑140 (MQ=255)
                                              cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                            >  1:1016444/1‑140 (MQ=255)
                                              cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                            >  1:2195555/1‑140 (MQ=255)
                                              cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                            >  1:1574618/1‑140 (MQ=255)
                                              cgcTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAg                                                                                            >  1:1097147/1‑140 (MQ=255)
                                                cTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGtt                                                                                          >  1:1557360/1‑140 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:1582091/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:281557/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:281558/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:1137529/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:1160237/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:1017627/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:1016444/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  1:1992335/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  1:2031822/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  1:491958/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  1:2383183/140‑1 (MQ=255)
                                                 tttCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  1:2749055/140‑1 (MQ=255)
                                                 tttCGATGCTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGttt                                                                                         <  2:284425/140‑1 (MQ=255)
                                                        gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                                  <  1:1202834/140‑1 (MQ=255)
                                                        gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                                  <  1:1402390/140‑1 (MQ=255)
                                                        gTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATg                                                                                  <  1:2637459/140‑1 (MQ=255)
                                                           tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                               >  2:3102139/1‑140 (MQ=255)
                                                           tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                               >  1:222001/1‑140 (MQ=255)
                                                           tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                               >  1:2516775/1‑140 (MQ=255)
                                                           tCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTAt                                                                               >  1:283462/1‑140 (MQ=255)
                                                             cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGCTCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                             <  2:1322257/140‑1 (MQ=255)
                                                             cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                             <  1:402818/140‑1 (MQ=255)
                                                             cGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATcc                                                                             <  2:1316652/140‑1 (MQ=255)
                                                                  ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGTTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                        <  2:106853/140‑1 (MQ=255)
                                                                  ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                        <  1:57919/140‑1 (MQ=255)
                                                                  ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                        <  1:2936143/140‑1 (MQ=255)
                                                                  ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                        <  2:1981210/140‑1 (MQ=255)
                                                                  ttGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCg                                                                        <  2:596104/140‑1 (MQ=255)
                                                                       tATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATc                                                                   <  1:2796606/140‑1 (MQ=255)
                                                                         tGATTTTCACTAGGGCGCAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCt                                                                 <  2:1614831/140‑1 (MQ=255)
                                                                            ttttCACGAGGGCGATTGATTCAGGTGATAGAATCGGCTAGGTGCTTGAAACGGAAGGGGATGAGAGGGAGGAAGGCATCATATCGGATAGTTTGTCGGGGAGGATTGAGTTTGAGGATGTATCCTTGCGTTATCCTGat                                                              <  2:134570/140‑2 (MQ=255)
                                                                               tCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATg                                                           >  2:1817384/1‑140 (MQ=255)
                                                                                 aCTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGa                                                         <  1:3102139/140‑1 (MQ=255)
                                                                                 aCTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGa                                                         <  2:22553/140‑1 (MQ=255)
                                                                                                 cAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCgg                                         >  1:45491/1‑140 (MQ=255)
                                                                                                 cAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCgg                                         >  1:2119865/1‑140 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  2:2444452/140‑1 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  2:2528970/140‑1 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  1:2804369/140‑1 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  2:1568964/140‑1 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  2:1302950/140‑1 (MQ=255)
                                                                                                  aGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGACGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGa                                        <  2:2830062/140‑1 (MQ=255)
                                                                                                       aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGcat                                                                                                                           <  2:447377/52‑1 (MQ=255)
                                                                                                       aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGcat                                                                                                                           >  1:447377/1‑52 (MQ=255)
                                                                                                       aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGtc                                   >  1:490762/1‑140 (MQ=255)
                                                                                                       aTAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGta                                   >  2:2259319/1‑139 (MQ=255)
                                                                                                         aGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGtctc                                 >  2:2769950/1‑140 (MQ=255)
                                                                                                         aGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGtctc                                 >  2:2658828/1‑140 (MQ=255)
                                                                                                            aTCGGCGAGGTGCCTGAAACGGACGGCGAGGAGCGGGAGGACGGCATCATATCTGATCGTTTGTCGGGGAGGATTGTGTTTCAGCATGTCTCCTTCCGTTATCCTGAAATGGACATAGAAGCGCTGCGGAATGTCTCAtt                              <  2:1471345/140‑1 (MQ=255)
                                                                                                             aacgCGAGGTGCTTGAAACGGAATTAAATGAGAGGTAGGAAGTCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCAttt                             >  2:2099685/4‑140 (MQ=255)
                                                                                                               ggCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTc                           >  2:474439/1‑140 (MQ=255)
                                                                                                                   aGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCa                       <  2:420988/140‑1 (MQ=255)
                                                                                                                   aGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCa                       <  2:423198/140‑1 (MQ=255)
                                                                                                                   aGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATCCTGAAATGGACAGAGAAGCGCTCAGGAATGTCTCATTTTCCGCa                       <  1:2196015/140‑1 (MQ=255)
                                                                                                                   aGGTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATCCTGAAATGGACAGAGAAGCGCTCAGGAATGTCTCATTTTCCGCa                       <  1:520159/140‑1 (MQ=255)
                                                                                                                     gTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCaaa                     >  1:2371641/1‑140 (MQ=255)
                                                                                                                     gTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATc                                                                   >  2:3008943/1‑94 (MQ=255)
                                                                                                                     gTGCTTGAAACAGAAGGCGACGAGCGGGAGGAAGGCACCATATCTGATCGTTTGTCAGGACGGATTGAGTTTCAGCATGTATCCTTTCGCTATc                                                                   <  1:3008943/94‑1 (MQ=255)
                                                                                                                         ttGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCg                 <  2:2195555/140‑1 (MQ=255)
                                                                                                                                ggAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTAATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGaaa          >  1:789301/1‑140 (MQ=255)
                                                                                                                                        aTGAGCGGGAGGAAGGCATCATATCGGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGAAACGATcgcg  <  2:892760/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GGTGATGTTGTTGCGATTATTAACTACGCAACACGAATTACGGGTGCGCTTTCGATGTTTCCGTTTTTGATTATGATTTTCACTAGGGCGAAGGCTTCAGGTGATAGAATCGGCGAGGTGCTTGAAACGGAAGGCGATGAGCGGGAGGAAGGCATCATATCTGATCGTTTGTCGGGGAGGATTGAGTTTCAGCATGTATCCTTCCGTTATCCTGAAATGGACAGAGAAGCGCTGCGGAATGTCTCATTTTCCGCAAAGCCGAGGGAAACGATCGCG  >  NZ_CP034943/871633‑871908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: