Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,635 C→A 11.0% T577K (ACA→AAA)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6350CA11.0% 303.9 / 5.1 131T577K (ACA→AAA) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (17/91);  new base A (18/2);  total (38/93)
Fisher's exact test for biased strand distribution p-value = 1.65e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

ATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTG  >  NZ_CP034943/1111499‑1111774
                                                                                                                                        |                                                                                                                                           
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  2:2184725/140‑1 (MQ=255)
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  1:1731697/140‑1 (MQ=255)
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  1:113540/140‑1 (MQ=255)
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  1:1502485/140‑1 (MQ=255)
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  2:388697/140‑1 (MQ=255)
aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTCTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  1:1611673/140‑1 (MQ=255)
 ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAttt                                                                                                                                         >  1:2393499/1‑140 (MQ=255)
 ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAttt                                                                                                                                         >  1:639631/1‑140 (MQ=255)
         cagcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTATGTAAt                                                                                                                                 >  1:2582662/1‑140 (MQ=255)
          agcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTCTAAtt                                                                                                                                >  2:3008325/1‑140 (MQ=255)
          agcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACGTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATCAAAtt                                                                                                                                >  2:647532/1‑140 (MQ=255)
          agcagcaTCAGCAGGAGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAtt                                                                                                                                <  1:3017228/140‑1 (MQ=255)
               caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                           <  1:313736/140‑1 (MQ=255)
               caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                           <  1:932088/140‑1 (MQ=255)
               caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTGTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                           <  1:1155765/140‑1 (MQ=255)
                   agcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcg                                                                                                                       <  1:1608175/140‑1 (MQ=255)
                   agcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcg                                                                                                                       <  1:299375/140‑1 (MQ=255)
                    gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCATCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                      <  1:1148099/140‑1 (MQ=255)
                    gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                      <  1:364019/140‑1 (MQ=255)
                    gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTGCATTTTCTGTTATTACCCCTgcgc                                                                                                                      >  1:2788976/1‑140 (MQ=255)
                        gCGTTGGCTGCAGTCCTTCAGCTCCTTGAGGAGTTTGTTGACATGCTGGGGGATGCTGAGAGTTCTCTTGATTTGGTTCCGCAAATTATGGAAGCGGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGa                                                                                                                  <  2:2032503/140‑1 (MQ=255)
                        gCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGAGGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTGAAAATCCGGCTGCGCGTGa                                                                                                                  >  2:3028083/1‑140 (MQ=255)
                         cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGAc                                                                                                                 <  2:1342372/140‑1 (MQ=255)
                             ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAGTTTGTCTAATTACGCCGGCGCTTGACCCgg                                                                                                             >  2:393082/1‑140 (MQ=255)
                               aTGCAGTCATTCAGCTAGTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAATTTAAATAATTACGCCAGAGCTTTATCACgtg                                                                                                           >  1:3108617/1‑140 (MQ=255)
                               aTGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAGTTTTAGCTAATTACGCCTGGGCGTGACCACgtg                                                                                                           >  2:2291001/1‑140 (MQ=255)
                               aTGCAGTCATTCAGCTACGTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCACTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGACTCGCTTAAAGTTTATATAAATACCGCTGCCCTTGAcccgcgg                                                                                                           >  2:1525220/1‑135 (MQ=255)
                                       aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                   <  1:760180/140‑1 (MQ=255)
                                       aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                   <  1:386681/140‑1 (MQ=255)
                                       aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                   <  1:1543461/140‑1 (MQ=255)
                                       aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                   <  2:639631/140‑1 (MQ=255)
                                            gCTACTGGCTGAGTTCGGTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                              <  2:370843/140‑1 (MQ=255)
                                             cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTATATAAATACGCCAGCGCTTGACCAGGGGTTTGTGGGCAatat                                                                                             >  2:677824/1‑140 (MQ=255)
                                             cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATATAAATAAGCCAGCGCTTTACCAAGTGTTTTTGGGCAatat                                                                                             >  1:673629/1‑140 (MQ=255)
                                             cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAAATTTATGTAATTACCCCTGCGCTTTAACACGGGTTTGTGGGccagat                                                                                             >  1:1684088/1‑135 (MQ=255)
                                             cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACAATTTGTGTAATTTAGCCTGCGGGTGAACAGGTGTTTTTGGGCCatat                                                                                             >  2:2744613/1‑140 (MQ=255)
                                               aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTGATTTTGTCTAAATAACCCTGCGCGTGACCCGGTGTGTGGGGGGAatattg                                                                                           >  1:529529/1‑138 (MQ=255)
                                                 ttGAGGAGTTTGTTGAAATGATGGGGGATGTTGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGAGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTGGGCAATATGGAt                                                                                         <  2:2101930/140‑1 (MQ=255)
                                                 ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                         <  2:1833697/140‑1 (MQ=255)
                                                 ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                         <  2:923065/140‑1 (MQ=255)
                                                 ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                         <  2:239021/140‑1 (MQ=255)
                                                 ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                         <  2:267777/140‑1 (MQ=255)
                                                       agTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                          <  1:2325460/85‑1 (MQ=255)
                                                       agTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATcc                                                                                   <  2:458091/140‑1 (MQ=255)
                                                       agTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATcc                                                                                   <  2:2551354/140‑1 (MQ=255)
                                                        gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                  <  2:226262/140‑1 (MQ=255)
                                                        gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                  <  2:1579256/140‑1 (MQ=255)
                                                        gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                  <  2:1533823/140‑1 (MQ=255)
                                                        gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                  <  2:269074/140‑1 (MQ=255)
                                                         tttGTTGAAATGATGGGGGGTGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGAGGAATTACCCCGGCGCGTGACCACGGGTTTGGCGGCCAAATGGATTTTTCCac                                                                                 >  2:1083855/1‑139 (MQ=255)
                                                          ttgttgAAATGATGGGGGATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                <  1:2416281/140‑1 (MQ=255)
                                                           tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATATAATTCCGCCAGCGCTTGACCACGTGTTTTTCGGCAAAATTGCTTTATACAGaa                                                                               >  2:746153/1‑140 (MQ=255)
                                                             tggAAATGATGGGGGCTGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTAGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATg                                                                             <  1:388924/138‑1 (MQ=255)
                                                                   tgatgGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                       <  1:1784827/140‑1 (MQ=255)
                                                                    gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                      <  1:2128869/140‑1 (MQ=255)
                                                                    gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                      <  1:1068807/140‑1 (MQ=255)
                                                                    gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                      <  1:2460402/140‑1 (MQ=255)
                                                                    gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                      <  1:1568983/140‑1 (MQ=255)
                                                                     atgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCAc                                                                     <  1:299514/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCTCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  1:2534633/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  1:1375081/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  2:2698192/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  1:1073237/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  1:2221906/140‑1 (MQ=255)
                                                                           gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                               <  1:2720374/140‑1 (MQ=255)
                                                                             tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                             <  1:1419494/140‑1 (MQ=255)
                                                                             tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                             <  1:1971652/140‑1 (MQ=255)
                                                                               atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAAATTTATATAATTAAGCCGGCGCTTTAACAGGGGTTTTTAGGCAAAATGGATTTATAAACAATATACGGCACCTCTTTCAcc                                                           >  2:1956506/1‑140 (MQ=255)
                                                                                tgaGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCt                                                          <  2:2184308/140‑1 (MQ=255)
                                                                                 gagacagctctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                         <  1:2506934/133‑1 (MQ=255)
                                                                                 gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATTTGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                         <  1:562673/140‑1 (MQ=255)
                                                                                 gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                         <  1:1956506/140‑1 (MQ=255)
                                                                                    aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTATGTAAATACGCCTGCGCATGTCCCCGGGTTTGTGGGCCAAATTGTGTTTTGCACAAAGTACGGCACCCCATGCACCGTtgt                                                      >  2:374521/1‑140 (MQ=255)
                                                                                    aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTATATAATTACCCCAGCGCTTGAACACGGGTTTTTAGGCAAAATTGTTTTATACAAAAAGTAAGGCAACACATGGACCGTtgt                                                      >  2:423101/1‑140 (MQ=255)
                                                                                    aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTGATTTTTTGTAATTAAGCCAGCGCGTGTCCCGGTGTTTTTTGGGAATATTTATTTTTAAAAAATTTTAAGCACCTCTTTGACCttttt                                                      >  1:2024356/1‑138 (MQ=255)
                                                                                      ttCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGc                                                    <  2:2304561/140‑1 (MQ=255)
                                                                                         tctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTCTCTAATTCCGCCAGCGCTTGACCAGGGGGTTGTCGGCAATATTGGTTTATCCAGAATGTACGGCACCGCTTGCACCGTtgtggggg                                                 >  2:1425636/1‑136 (MQ=255)
                                                                                              aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTATCTAATTACGCCAGCGCTTGACCAGGGGTTTGTCGGCAATATTGATTTATCCAGAATGTACGGCACCGCTTGCACCGGTGTGCTGGGGGCa                                            >  2:628657/1‑140 (MQ=255)
                                                                                              aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTCTCTAATTCCGCCTGCGCGTGACCAGGGGTTTGTCGGGAAAATGGATTTATACAGAATGTACGGCACCGCTTGCACCGTTGTTCTGGGGGCa                                            >  2:1560903/1‑140 (MQ=255)
                                                                                                ttGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCaaa                                          <  2:1684088/140‑1 (MQ=255)
                                                                                                ttGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCaaa                                          <  2:2341068/140‑1 (MQ=255)
                                                                                                ttGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCaaa                                          <  2:673629/140‑1 (MQ=255)
                                                                                                    ttCCGCAAATGATGGAAGCGGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                      <  1:2790322/140‑1 (MQ=255)
                                                                                                    ttCAGCAAATGATGGAAGCCGGCGCTGAGTCGCTTAAATTTTCTCTAATTCCGCGTGCGCTTGACCAGGTGTTTTTCGGCAATATGGATTTATCCAGATTGTAGGGCACCTCTTGCACGTTTGTGCTGGGGGCAAacgac                                      <  1:1969828/140‑1 (MQ=255)
                                                                                                    ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                      <  1:327044/140‑1 (MQ=255)
                                                                                                    ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                      <  1:1011438/140‑1 (MQ=255)
                                                                                                    ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                      <  1:832713/140‑1 (MQ=255)
                                                                                                    ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGGTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                      <  2:270393/140‑1 (MQ=255)
                                                                                                          aaaTGATGGAAGCCGGCGCGGAGTCGCGTCCATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGTTTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGtt                                <  1:1992425/140‑1 (MQ=255)
                                                                                                            atgatgGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTct                              <  2:671067/140‑1 (MQ=255)
                                                                                                            atgatgGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTct                              <  2:1620307/140‑1 (MQ=255)
                                                                                                            atgatgGAAGCCGGCGCGGAGTCGCTTAAATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAAGATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTct                              <  2:517245/140‑1 (MQ=255)
                                                                                                                  gAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                        <  2:529529/140‑1 (MQ=255)
                                                                                                                                   gCTTACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATg       <  1:1892386/140‑1 (MQ=255)
                                                                                                                                      tACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATgggg    <  1:1423263/140‑1 (MQ=255)
                                                                                                                                      tACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATgggg    <  1:2263050/140‑1 (MQ=255)
                                                                                                                                      tACATTGTCTCTAATTCCGCGGGCGCTTGACCAGGTGTGTGTCGGCACTATGGATTTATCCAGAATGTACGGCACCGCTTGCACCTTTGTGCTGGGGGCAAACGACGGCTTTCTGCCTGCACGCCCTGATGCAAATgggg    <  1:561765/140‑1 (MQ=255)
                                                                                                                                        cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg  <  2:3108617/140‑1 (MQ=255)
                                                                                                                                        cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGGTTGTCCGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACAGTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCAGGATGAAAAGGGGGTg  <  2:866479/140‑1 (MQ=255)
                                                                                                                                        cATTTTCTCTAATTCCGCCGGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg  <  2:2336226/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTG  >  NZ_CP034943/1111499‑1111774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: