Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,112,969 C→T 100% L1022F (CTT→TTT)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,112,9690CT99.3% 489.3 / ‑4.5 149L1022F (CTT→TTT) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (74/74);  total (75/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 150 total aligned reads displayed.

ACGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCT  >  NZ_CP034943/1112832‑1113104
                                                                                                                                         |                                                                                                                                       
aCGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGttt                                                                                                                                       <  2:2235264/140‑1 (MQ=255)
 cGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTg                                                                                                                                      >  2:1184890/1‑140 (MQ=255)
    actagagcTGCAAATGCTGACGTACCTAGATTAATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGCAGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGc                                                                                                                                   >  2:2824029/7‑140 (MQ=255)
    aCTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGc                                                                                                                                   >  1:1375500/1‑140 (MQ=255)
      tGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCtt                                                                                                                                 >  1:1098223/1‑140 (MQ=255)
        gcgcTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGCCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGa                                                                                                                               >  2:1727971/1‑140 (MQ=255)
        gcgcTGCAAATGCAGACTTACCTTGATTTAGCGATTACTCATTCAGACGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAAACTCCCGTTTGGGCTTgc                                                                                                                               >  1:785014/1‑139 (MQ=255)
             gCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACgaga                                                                                                                          <  1:2510898/140‑1 (MQ=255)
             gCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACgaga                                                                                                                          <  1:706924/140‑1 (MQ=255)
              cAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTACTGTAATTCCAAAAACAAGACCCGATGATTCAATCTAATCTCCCCTTTGGGAATGAAGAGAt                                                                                                                         >  2:221008/1‑140 (MQ=255)
              cAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCCGCAGGACAGCTGTATATCCAAATACATGACCCGAAGATTCAATATAATCTACCGTATGGGCTTGAccacat                                                                                                                         >  2:159788/1‑135 (MQ=255)
                  tGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGaa                                                                                                                     <  2:641121/140‑1 (MQ=255)
                  tGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGaa                                                                                                                     <  2:1363249/140‑1 (MQ=255)
                  tGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGaa                                                                                                                     <  2:2370302/140‑1 (MQ=255)
                      gACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAgg                                                                                                                 <  1:683455/140‑1 (MQ=255)
                      gACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAgg                                                                                                                 <  1:2263949/140‑1 (MQ=255)
                      gACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAgg                                                                                                                 <  1:2541432/140‑1 (MQ=255)
                       aCGTACCTTGATTTATCGAGTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGga                                                                                                                <  2:1102546/140‑1 (MQ=255)
                                 aTTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACTCCGGAAGGAGTGCTGTATTTCCATATACATTACCAGATGATTAAATCTAATCTCCAGTATGGTCTTTAAGAGATTGAAAATGATATCTTTaaa                                                                                                      >  2:2043333/1‑139 (MQ=255)
                                     aTCGATTACTCATTCAGCCGACTCGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGAAGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGAATGAACAGGAGATCATTAAGAAAt                                                                                                  >  2:547850/1‑140 (MQ=255)
                                      tCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAAtt                                                                                                 >  1:1239270/1‑140 (MQ=255)
                                       cGATTACTCATTCAGCCGACTGGCTCGGAATGAGTGCGACGCCGGCAGGAGTGCTGTATTTCCATATACAAGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGAAGAGATTTAACAGTATATCTTTAAGAAAtaa                                                                                                >  2:2935887/1‑138 (MQ=255)
                                       cGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGTGCTTGACGATATTGAACAGGAGATCTTTAAGAAAttt                                                                                                >  2:501040/1‑140 (MQ=255)
                                           tACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTcaaa                                                                                            >  1:109031/1‑140 (MQ=255)
                                             cTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATg                                                                                          <  1:1372223/140‑1 (MQ=255)
                                                      ccGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTg                                                                                 >  2:2815702/1‑140 (MQ=255)
                                                         aCTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGctc                                                                              >  2:1880857/1‑140 (MQ=255)
                                                         aCTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGctc                                                                              >  2:403900/1‑140 (MQ=255)
                                                                cGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCg                                                                       <  1:2032907/140‑1 (MQ=255)
                                                                    aTGAGGGCGCCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAAACTCCCCTTTGGGCATGACGAGATTGAACAGGAGATCTTTCAGAAATATAAAATGAAGGCATTGCTCCTCCGCGATCa                                                                   >  2:2139668/1‑140 (MQ=255)
                                                                       aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGa                                                                <  2:3098679/140‑1 (MQ=255)
                                                                       aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGa                                                                >  1:1567126/1‑140 (MQ=255)
                                                                       aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGa                                                                <  2:309134/140‑1 (MQ=255)
                                                                       aGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAACGGCTTGCTCCTCGGCGATCAGGa                                                                <  2:1940875/140‑1 (MQ=255)
                                                                             aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                          <  2:1587171/140‑1 (MQ=255)
                                                                             aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                          <  1:692417/140‑1 (MQ=255)
                                                                             aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                          >  2:1104681/1‑140 (MQ=255)
                                                                             aCGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTATAAGAAATTTAAAATGAAGTGCTTGCTCCTCGGCGATCAGGAACCGGt                                                          >  2:1268768/1‑140 (MQ=255)
                                                                              cGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGtt                                                         <  2:1637054/140‑1 (MQ=255)
                                                                              cGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGtt                                                         <  2:2375280/140‑1 (MQ=255)
                                                                              cGCCGGCAGGAGGGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGTGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGtt                                                         <  2:2373864/140‑1 (MQ=255)
                                                                                ccGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCg                                                       <  2:495948/140‑1 (MQ=255)
                                                                                    cAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCtt                                                   <  1:984145/140‑1 (MQ=255)
                                                                                    cAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCtt                                                   <  1:634023/140‑1 (MQ=255)
                                                                                    cAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGATTCGCCtt                                                   <  2:611618/140‑1 (MQ=255)
                                                                                     aGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTa                                                  <  2:832191/140‑1 (MQ=255)
                                                                                     aGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTa                                                  <  1:403900/140‑1 (MQ=255)
                                                                                           gCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATa                                            >  2:1089986/1‑140 (MQ=255)
                                                                                           gCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATATTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATAAGTAATCGGTTCTCCTTAtctata                                            >  2:1315783/1‑135 (MQ=255)
                                                                                            cTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATAc                                           <  1:2455483/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:21565/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:2808207/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:21940/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:325544/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:2418897/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:1736394/140‑1 (MQ=255)
                                                                                              gTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGTGTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  2:2419244/140‑1 (MQ=255)
                                                                                                 tttCCATATACATGACCCGATGATTCAATCTAATCTCCCGTGTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAACATGAAGGGCGTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGAccc                                      <  2:785014/140‑1 (MQ=255)
                                                                                                      atatACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCaa                                 >  1:2343184/1‑140 (MQ=255)
                                                                                                      atatACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCaa                                 >  1:3006061/1‑140 (MQ=255)
                                                                                                      atatACATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCaa                                 >  1:589137/1‑140 (MQ=255)
                                                                                                       tataCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAag                                >  2:124526/1‑140 (MQ=255)
                                                                                                       tataCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCATTATGGATACGACCCTTCAag                                >  2:378253/1‑140 (MQ=255)
                                                                                                       tataCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGACTTATGGATAAGAACCTACaaa                                >  2:2712542/1‑139 (MQ=255)
                                                                                                       tataCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGAGGTTCGCCTTATGGATACGACCCTACaat                                >  2:378373/1‑139 (MQ=255)
                                                                                                         taCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGCTCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAagag                              >  1:1797754/1‑140 (MQ=255)
                                                                                                         taCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAagag                              >  2:2401009/1‑140 (MQ=255)
                                                                                                         taCATGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTAGGGATACGACCCTTCAagag                              >  1:1300454/1‑140 (MQ=255)
                                                                                                           cATGAACCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAggg                            >  2:945605/1‑140 (MQ=255)
                                                                                                            aTGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATTGATACGACACTTCAACAGGGa                           >  2:2701132/1‑140 (MQ=255)
                                                                                                            aTGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGa                           <  2:1239270/140‑1 (MQ=255)
                                                                                                            aTGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGAGACGACCCTTCAAGAGGGa                           >  1:2965734/1‑140 (MQ=255)
                                                                                                            aTGACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCACGAAGCGGTTCGACTTATGGATACGACACTTCAAGAGGGa                           >  2:2004818/1‑140 (MQ=255)
                                                                                                            aTGACCCGAAGAATCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGa                           <  2:2172850/140‑1 (MQ=255)
                                                                                                              gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         <  1:2654138/124‑1 (MQ=255)
                                                                                                              gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         >  2:2653929/1‑124 (MQ=255)
                                                                                                              gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGa                                         >  2:2654138/1‑124 (MQ=255)
                                                                                                              gACCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACg                         >  1:2902334/1‑140 (MQ=255)
                                                                                                              gACCCGATGATTCAATCCAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACg                                          >  1:2916206/1‑123 (MQ=255)
                                                                                                               aCCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACgg                        >  2:336478/1‑140 (MQ=255)
                                                                                                               aCCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACgg                        >  1:1956470/1‑140 (MQ=255)
                                                                                                               aCCCGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGCCTTGCACCTCGGCGATCAGGAAGAGGTTCGCCTTATGTATACGACCCTTCAAGAGGGACgg                        >  2:1199749/1‑140 (MQ=255)
                                                                                                               aCCCGATGATTCAATCTAATCTACCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACgg                        >  1:162560/1‑140 (MQ=255)
                                                                                                               aCCCGATGATTCAATCTAATCTACAGTTTGGGCTTGACGAGATTGAACACGAGATCTTTAAGAAAGTTAAAATGAAGGGCTAGCTCCTCGGCGATCATGAAGAGGTTCTCCTTATGGATACCAACCTTCAAGATGTACgg                        >  2:2121912/1‑140 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  1:2069278/140‑1 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  1:788554/140‑1 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  1:2688747/140‑1 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  2:2454370/140‑1 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  1:1217742/140‑1 (MQ=255)
                                                                                                                cccGATGATTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAAGGAATGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  2:2216012/140‑1 (MQ=255)
                                                                                                                   gatgatTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                          <  1:1996665/102‑1 (MQ=255)
                                                                                                                   gatgatTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGt                                                          >  2:1996665/1‑102 (MQ=255)
                                                                                                                   gatgatTCAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCaa                    >  1:1842620/1‑140 (MQ=255)
                                                                                                                          cAATCTAATCTCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATatcat             <  1:2412028/140‑1 (MQ=255)
                                                                                                                           aatctaatctCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       >  1:1404174/1‑129 (MQ=255)
                                                                                                                           aatctaatctCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGt                       <  2:1404174/129‑1 (MQ=255)
                                                                                                                               taatctCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATg        <  2:449570/140‑1 (MQ=255)
                                                                                                                                 atctCCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCATCGGCGATCACGAAGCGGTACGCCTTATGGATACGACCCTTCAAGACGGACGGACAAATATCATCAAAGCg      >  2:2445371/1‑140 (MQ=255)
                                                                                                                                  tctcCCGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCgg     >  2:1813659/1‑140 (MQ=255)
                                                                                                                                     cccGTTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCt  <  2:2236705/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ACGGACTGGCGCTGCAAATGCTGACGTACCTTGATTTATCGATTACTCATTCAGCCGACTGGCTCGGAATGAGGGCGACGCCGGCAGGAGTGCTGTATTTCCATATACATGACCCGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTGCTCCTCGGCGATCAGGAAGCGGTTCGCCTTATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCGGGCT  >  NZ_CP034943/1112832‑1113104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: