Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,136,748 T→C 5.5% F172F (TTT→TTC EO946_RS05840 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,136,7480TC 5.5% 673.8 / 17.2 203F172F (TTT→TTCEO946_RS05840AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (65/125);  new base C (5/6);  total (70/133)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

AAGGCAAGGTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCTGCATGCAGC  >  NZ_CP034943/1136609‑1136887
                                                                                                                                           |                                                                                                                                           
aaGGCAAGGTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGttt                                                                                                                                             <  2:2266706/140‑1 (MQ=255)
      aGGTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAAtt                                                                                                                                       >  1:1137725/1‑140 (MQ=255)
        gTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAAGCTGATCGATTACTCCGCGGAAAACTCTGATTTGCCGCTCAGAAAGCAAATTTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAAGCGTTCCAAATTcc                                                                                                                                     >  1:95844/1‑140 (MQ=255)
        gTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAAGCTGATCGATTACTCCGCGGAAAACTCTGATTTGCCGCTCAGAAAGCAAATTTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAAGCGTTCCAAATTcc                                                                                                                                     >  1:188794/1‑140 (MQ=255)
        gTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAAGCTGATCGATTACTCCGCGGAAAACTCTGATTTGCCGCTCAGAAAGCAAATTTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAAGCGTTCCAAATTcc                                                                                                                                     >  1:1767679/1‑140 (MQ=255)
        gTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAAGCTGATCGATTACTCCGCGGAAAACTCTGATTTGCCGCTCAGAAAGCAAATTTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAAGCGTTCCAAATAcc                                                                                                                                     >  1:1572905/1‑140 (MQ=255)
            aGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCt                                                                                                                                 >  2:1566172/1‑140 (MQ=255)
            aGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCt                                                                                                                                 >  2:2296355/1‑140 (MQ=255)
            aGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCt                                                                                                                                 >  2:2465913/1‑140 (MQ=255)
            aGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCt                                                                                                                                 >  2:672270/1‑140 (MQ=255)
                   gCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAag                                                                                                                          >  2:2154482/1‑140 (MQ=255)
                   gCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAag                                                                                                                          >  2:368190/1‑140 (MQ=255)
                   gCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAag                                                                                                                          >  2:808112/1‑140 (MQ=255)
                    cAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAaga                                                                                                                         <  1:2296355/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:1387534/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:1297666/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:1661026/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:1014590/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:438079/140‑1 (MQ=255)
                        ttATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCgg                                                                                                                     <  2:875754/140‑1 (MQ=255)
                         tATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAACACTCTGATTTGCCGCTTAGCAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCggg                                                                                                                    <  2:2830654/140‑1 (MQ=255)
                         tATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAACACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCggg                                                                                                                    <  2:2081263/140‑1 (MQ=255)
                         gagggCGCCGCAGTTTGAAAGGCTGATAGATTACTCCGCCGACAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCggg                                                                                                                    <  2:2041490/137‑1 (MQ=255)
                          aTGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGt                                                                                                                   <  1:1361494/140‑1 (MQ=255)
                          aTGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGt                                                                                                                   <  1:2157670/140‑1 (MQ=255)
                           tGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTa                                                                                                                  >  1:1045520/1‑140 (MQ=255)
                           tGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTa                                                                                                                  >  1:2882371/1‑140 (MQ=255)
                           tGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTa                                                                                                                  >  1:2368254/1‑140 (MQ=255)
                           tGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTGTCAAATTCCGTCTGCGAAAGAGCGGGTa                                                                                                                  >  1:2778555/1‑140 (MQ=255)
                                 agcagTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTgg                                                                                                            >  2:1844271/1‑140 (MQ=255)
                                 agcagTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTgg                                                                                                            >  1:1810766/1‑140 (MQ=255)
                                 agcagTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTgg                                                                                                            >  1:3076079/1‑140 (MQ=255)
                                 agcagTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTgg                                                                                                            >  1:241542/1‑140 (MQ=255)
                                   cagTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGa                                                                                                          >  2:2460298/1‑140 (MQ=255)
                                     gTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAgg                                                                                                        <  2:2619501/140‑1 (MQ=255)
                                     gTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAgg                                                                                                        <  2:744149/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  2:271515/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  2:1251762/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  2:292681/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  2:917514/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  1:434333/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  1:2031857/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  1:2841732/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  1:1976284/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  1:2431575/140‑1 (MQ=255)
                                          aaaGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGGTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGcc                                                                                                   <  2:1528665/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:318215/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  2:241542/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:839378/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:822122/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:1578328/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:1699629/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:1199242/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:2917970/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  1:2150400/140‑1 (MQ=255)
                                            aGGCTGATAGATTACTCCGATGAAAAGTCTTATTTGCCGCTGAGAAAGCAAATCTTATTTGAGGAGCTGATGCTTCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCg                                                                                                 <  2:1397903/140‑1 (MQ=255)
                                                        tACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGa                                                                                     >  1:480384/1‑140 (MQ=255)
                                                         aCTCCGCGGAAAACTCTGAGTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAg                                                                                    <  2:2589546/140‑1 (MQ=255)
                                                             cgcgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCCtt                                                                                >  1:2187606/1‑140 (MQ=255)
                                                             cgcgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCAtt                                                                                >  1:921489/1‑140 (MQ=255)
                                                             cgcgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCAtt                                                                                >  2:2243423/1‑140 (MQ=255)
                                                             cgcgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCAtt                                                                                >  1:1843502/1‑140 (MQ=255)
                                                             cgcgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCAtt                                                                                >  1:2490342/1‑140 (MQ=255)
                                                               cgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAaga                                                                                                                         >  2:648864/1‑97 (MQ=255)
                                                               cgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAaga                                                                                                                         <  1:648864/97‑1 (MQ=255)
                                                               cgGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATtat                                                                              >  2:2362617/1‑140 (MQ=255)
                                                                                ccGCTTAGAAAGCAACTCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAAt                                                             <  2:1842668/140‑1 (MQ=255)
                                                                                ccGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAAt                                                             <  2:2802358/140‑1 (MQ=255)
                                                                                ccGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTACAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAAt                                                             <  2:3055438/140‑1 (MQ=255)
                                                                                               aTCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTctcgct                                              <  1:2066648/140‑1 (MQ=255)
                                                                                               aTCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATCATAAGGAAAAGACGACAATCAAAGATCTctcgct                                              <  2:940627/140‑1 (MQ=255)
                                                                                                tCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAAGTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTctcgctc                                             <  2:1220068/140‑1 (MQ=255)
                                                                                                 cTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTctcgctcg                                            <  2:2972307/140‑1 (MQ=255)
                                                                                                 cTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTctcgctcg                                            <  1:1236140/140‑1 (MQ=255)
                                                                                                 cTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTctcgctcg                                            <  1:2119067/140‑1 (MQ=255)
                                                                                                   tATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGcc                                          <  2:1137725/140‑1 (MQ=255)
                                                                                                      ttGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCtt                                       <  2:344607/140‑1 (MQ=255)
                                                                                                      ttGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCtt                                       <  2:1779713/140‑1 (MQ=255)
                                                                                                          ggagCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACt                                   <  1:2124598/140‑1 (MQ=255)
                                                                                                           gagCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTa                                  >  2:1730212/1‑140 (MQ=255)
                                                                                                           gagCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTa                                  >  2:157292/1‑140 (MQ=255)
                                                                                                              cTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTatca                               >  2:599322/1‑140 (MQ=255)
                                                                                                               tGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTatcat                              <  1:2243423/140‑1 (MQ=255)
                                                                                                               tGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTatcat                              <  1:3020458/140‑1 (MQ=255)
                                                                                                               tGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGAGGCCCGGTAATTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATATCTCGCTCGCCCATCACAatcaa                              >  2:1235309/1‑139 (MQ=255)
                                                                                                                    ctgcATCTTCAAAAAGAGGCGTTTCAAAGTCCGTCTGCGAAAGAGCGGGTAGCCTGGGCGGCGGCCCGGTACATACAGGAGCATTATAAGGAAAAGACGACAATCAAAGAACTCTCGCTCGCCCTTCACTATCATCAGGa                         <  2:2448764/140‑1 (MQ=255)
                                                                                                                      gcATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGAtt                       >  2:500258/1‑140 (MQ=255)
                                                                                                                       cATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAg                                                                                       >  1:1412959/1‑75 (MQ=255)
                                                                                                                        aTCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTAt                     <  2:2187606/140‑1 (MQ=255)
                                                                                                                           ttCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTAtgtg                  >  1:2152245/1‑140 (MQ=255)
                                                                                                                              aaaaaGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACGATCATCAGGCTTATGTGAGc               >  1:2055161/1‑140 (MQ=255)
                                                                                                                              aaaaaGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCAGTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGc               >  1:1522550/1‑140 (MQ=255)
                                                                                                                                caagaagCGTTCCAAAGTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCg             <  2:188794/139‑1 (MQ=255)
                                                                                                                                    agGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTatcat                              <  2:2123954/119‑1 (MQ=255)
                                                                                                                                    agGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTatcat                              <  2:2386129/119‑1 (MQ=255)
                                                                                                                                     ggCGTTTCAAATTCCGTCTGCGACAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCtgca        <  2:2882371/140‑1 (MQ=255)
                                                                                                                                     ggCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCtgca        <  1:377/140‑1 (MQ=255)
                                                                                                                                     ggCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCtgca        <  2:2778555/140‑1 (MQ=255)
                                                                                                                                        gTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCtgcatgc     >  1:574989/1‑140 (MQ=255)
                                                                                                                                           tCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCTGCATgcagc  <  1:599322/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAGGCAAGGTGCAGCGCCCGCAGTTTATGGCGCAGCAGTTTGAAAGGCTGATAGATTACTCCGCGGAAAACTCTGATTTGCCGCTTAGAAAGCAAATCTTATTTGAGGAGCTGATGCTGCATCTTCAAAAAGAGGCGTTTCAAATTCCGTCTGCGAAAGAGCGGGTAGCCTGGGAGGCGGCCCGGTACTTACAGGAGCATTATAAGGAAAAGACGACAATCAAAGATCTCTCGCTCGCCCTTCACTATCATCAGGATTATGTGAGCCGCTGCATGCAGC  >  NZ_CP034943/1136609‑1136887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: