Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,176,173 G→T 91.9% G135V (GGT→GTT)  med → transcriptional regulator Med

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,176,1730GT91.9% 495.9 / 21.3 167G135V (GGT→GTT) medtranscriptional regulator Med
Reads supporting (aligned to +/- strand):  ref base G (7/8);  new base T (84/67);  total (91/76)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CTCTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAACACA  >  NZ_CP034943/1176034‑1176312
                                                                                                                                           |                                                                                                                                           
         cATCGGAGTGAAGATGCAGAGGTGTTGAACTAGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgg                                                                                                                                    <  1:1622908/140‑1 (MQ=255)
          aTGGGAGTGAATCTGCAGAGGTGTTTAACTGGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgga                                                                                                                                   <  2:1890695/140‑1 (MQ=255)
          aTGGGAGTGAATATGCAGAGGTGTTTAACTTTGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTgga                                                                                                                                   <  2:1406043/140‑1 (MQ=255)
           tGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggag                                                                                                                                  >  1:1173629/1‑140 (MQ=255)
             ggAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggagga                                                                                                                                >  1:125678/1‑140 (MQ=255)
             ggAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTggagga                                                                                                                                >  1:443175/1‑140 (MQ=255)
              gAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGaa                                                                                                                               <  1:797063/140‑1 (MQ=255)
               attGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAAt                                                                                                                              <  2:2843558/138‑1 (MQ=255)
                gTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATg                                                                                                                             >  1:2960029/1‑140 (MQ=255)
                 tgcatatGCCGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATGCGTCAATTCTAATGCTAACGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGACGCCATGGTTTTTTTTGGAGGAATgc                                                                                                                            >  2:2649717/4‑139 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGACTGa                                                                                                                            >  2:1660571/1‑140 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGACTGa                                                                                                                            >  2:2022349/1‑140 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGa                                                                                                                            >  1:1512397/1‑140 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGa                                                                                                                            >  2:2717150/1‑140 (MQ=255)
                 tGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGa                                                                                                                            >  1:1708004/1‑140 (MQ=255)
                  gAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                           <  1:2796485/140‑1 (MQ=255)
                  gAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                           >  2:2035753/1‑140 (MQ=255)
                  gAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                           >  2:1947469/1‑140 (MQ=255)
                    atatGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAg                                                                                                                         <  1:2967243/140‑1 (MQ=255)
                       tGCCGAGGTGTTTAACTGGCTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATCTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTg                                                                                                                      <  2:706926/140‑1 (MQ=255)
                       tGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGCTATGGAATTCGTCATTTCTAATGCTAAAGCCAAACCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGCATGACAGCTg                                                                                                                      <  2:648315/140‑1 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGACTGACAGCTGc                                                                                                                     >  1:2281405/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGc                                                                                                                     >  1:2234464/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGc                                                                                                                     >  2:2265373/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGc                                                                                                                     >  2:2534760/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGc                                                                                                                     >  1:610628/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGc                                                                                                                     >  2:1046729/1‑140 (MQ=255)
                        gCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCAATTTCAGCGGAGAAGCAATGGGTTTTTTTGGAGGAATGAAAGCTGc                                                                                                                     >  2:929313/1‑140 (MQ=255)
                         cAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGcc                                                                                                                    >  1:1078683/1‑140 (MQ=255)
                             ggTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATc                                                                                                                >  2:1029121/1‑140 (MQ=255)
                              gtgtTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAATCTGATAATTTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACATCTGCCCATCt                                                                                                               >  1:2316340/1‑140 (MQ=255)
                                   tAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACACCTGCCCATCTGTCGa                                                                                                          >  2:262801/1‑140 (MQ=255)
                                      cTTGGTCAGCGAAGATTCTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGCAGCCATGGTTTTGTTTGGAGGAATGACAGCTGCCCCTCTGTCGcaaa                                                                                                       >  2:2504181/1‑140 (MQ=255)
                                      cTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGaaaa                                                                                                       <  1:55127/140‑1 (MQ=255)
                                        tGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACg                                                                                                     >  1:3022518/1‑140 (MQ=255)
                                         tgTCAGCGAAGATTATCCAGATAAGGAATTCGTCATTTCTAATGCTAAAGAAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGAAATGGTATTTTTTCGCGGAATGAAAGCTGACCATCAGACGAAAACGa                                                                                                    >  2:1991930/2‑140 (MQ=255)
                                         ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTTGAGGAATGACAGCTGCCCATCTGTCGAAAActa                                                                                                    >  1:1742724/1‑138 (MQ=255)
                                         ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                           <  2:2996016/117‑1 (MQ=255)
                                         ggTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGa                                                                                                    >  1:421127/1‑140 (MQ=255)
                                           tCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCACTGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAAt                                                                                                  >  2:2186047/1‑140 (MQ=255)
                                           tCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAAt                                                                                                  >  2:2858124/1‑140 (MQ=255)
                                                gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                             <  2:389709/140‑1 (MQ=255)
                                                gAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                             <  1:653586/140‑1 (MQ=255)
                                                     ttATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCg                                                                                        <  2:2955033/140‑1 (MQ=255)
                                                     ttATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTATTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCg                                                                                        <  2:820922/140‑1 (MQ=255)
                                                       aTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                      >  1:1026282/1‑140 (MQ=255)
                                                       aTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                      >  1:1472841/1‑140 (MQ=255)
                                                       aTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                      >  1:1428328/1‑140 (MQ=255)
                                                       aTCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTg                                                                                      >  1:2529221/1‑140 (MQ=255)
                                                        tCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGa                                                                                     >  1:2016204/1‑140 (MQ=255)
                                                        tCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGa                                                                                     <  2:1645929/140‑1 (MQ=255)
                                                            gATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGc                                                                                 <  2:1274715/140‑1 (MQ=255)
                                                             atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                                >  1:2362532/1‑140 (MQ=255)
                                                             atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                                >  1:2973546/1‑140 (MQ=255)
                                                             atatGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCt                                                                                >  1:2461511/1‑140 (MQ=255)
                                                               atGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTc                                                                              <  1:1773364/140‑1 (MQ=255)
                                                               atGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTc                                                                              <  1:866152/140‑1 (MQ=255)
                                                                  gAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCtt                                                                           >  2:1796520/1‑140 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGACGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                      >  1:1274402/1‑140 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGcc                                                                                                                    >  2:809015/1‑94 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGcc                                                                                                                    <  1:809015/94‑1 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                      >  1:2447915/1‑140 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                      >  1:415771/1‑140 (MQ=255)
                                                                       cGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGt                                                                      >  2:1240928/1‑140 (MQ=255)
                                                                             ttCTAATGCTAAAGCAATAGCTTTTATTGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGTATCAGGTCGGCGTGATTGCGTCCTGTACGTGGCAGc                                                                <  2:2316340/140‑1 (MQ=255)
                                                                             ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGACGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                                <  2:439889/140‑1 (MQ=255)
                                                                             ttCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGc                                                                <  2:2885292/140‑1 (MQ=255)
                                                                                      tAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                       <  1:2821224/140‑1 (MQ=255)
                                                                                      tAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAg                                                       <  2:1522230/140‑1 (MQ=255)
                                                                                       aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      <  2:528160/140‑1 (MQ=255)
                                                                                       aaaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGa                                                      <  2:459648/140‑1 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:296706/1‑140 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGCCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:1808755/1‑140 (MQ=255)
                                                                                        aaGCAAAAGCTGATAATGTGACGAGGGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGAc                                                     >  2:1393111/1‑140 (MQ=255)
                                                                                              aaGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCACTGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGtttt                                               >  2:2678745/1‑140 (MQ=255)
                                                                                               aGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAAt                                                                                                                              >  2:2965309/1‑60 (MQ=255)
                                                                                                    aTAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCaaa                                         <  2:125678/140‑1 (MQ=255)
                                                                                                           gACGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGAc                                                                                                                           >  2:1183801/1‑51 (MQ=255)
                                                                                                            aCGAGTGTCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCaa                                 <  1:2883760/140‑1 (MQ=255)
                                                                                                                 tgtCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGCATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTGACGTGGCAGCCGGAGGTCGACGGTTTTCTCAAAGGGGCCAAATATg                            >  2:2334346/1‑140 (MQ=255)
                                                                                                                 tgtCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAatatt                            >  2:868276/1‑139 (MQ=255)
                                                                                                                 tgtCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATg                            >  2:2334600/1‑140 (MQ=255)
                                                                                                                 tgtCCATTTCAGCGGCGAAGCAATGGGTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGa                           >  2:2807058/1‑140 (MQ=255)
                                                                                                                   tCCATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGt                                                                                             <  1:2979617/73‑1 (MQ=255)
                                                                                                                     cATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaaaa                        <  2:2447915/140‑1 (MQ=255)
                                                                                                                     cATTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGaaaa                        <  2:415771/140‑1 (MQ=255)
                                                                                                                      aTTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                       <  2:157861/140‑1 (MQ=255)
                                                                                                                      aTTTCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAAt                       <  1:1760180/140‑1 (MQ=255)
                                                                                                                        ttCAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATcc                     <  2:1222452/140‑1 (MQ=255)
                                                                                                                          cAGCGGCGAATCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCgg                   <  1:3025/140‑1 (MQ=255)
                                                                                                                          cAGCGGCGAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCgg                   <  1:1982209/140‑1 (MQ=255)
                                                                                                                             cggcgAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGata                >  2:1131160/1‑140 (MQ=255)
                                                                                                                               gcgAAGCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGAtata              <  1:1523388/140‑1 (MQ=255)
                                                                                                                                    gCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGt         <  1:2332878/140‑1 (MQ=255)
                                                                                                                                    gCAATGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGt         <  1:142237/140‑1 (MQ=255)
                                                                                                                                    gCAAGGGTTTTTTTGGGAGGAATGACAGCTGCCCATCTGTCGAACACGAATCAGGTCGGCGTGATTGCTACCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGt         <  1:513167/140‑1 (MQ=255)
                                                                                                                                        tGGTTTTTTTTGGAGGAATGAGAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac     <  1:1849018/140‑1 (MQ=255)
                                                                                                                                        tGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac     <  2:1078683/140‑1 (MQ=255)
                                                                                                                                        tGGTTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAac     <  1:59131/140‑1 (MQ=255)
                                                                                                                                           gTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAAcaca  <  1:2591470/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTCTATGGCCATGGGAGTGAATATGCAGAGGTGTTTAACTTGGTCAGCGAAGATTATCCAGATATGGAATTCGTCATTTCTAATGCTAAAGCAAAAGCTGATAATGTGACGAGTGTCCATTTCAGCGGCGAAGCAATGGGTTTTTTTGGAGGAATGACAGCTGCCCATCTGTCGAAAACGAATCAGGTCGGCGTGATTGCTTCCTTTACGTGGCAGCCGGAGGTAGACGGTTTTATCAAAGGGGCCAAATATGAAAATCCGGATATAGAAGTCAACACA  >  NZ_CP034943/1176034‑1176312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: