Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,347,639 T→G 5.0% L175L (CTT→CTG purU → formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,347,6390TG 5.0% 654.6 / 15.0 198L175L (CTT→CTGpurUformyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base T (66/122);  new base G (5/5);  total (71/127)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 198 total aligned reads displayed.

TATGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATA  >  NZ_CP034943/1347501‑1347776
                                                                                                                                          |                                                                                                                                         
tATGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTg                                                                                                                                          <  1:991939/140‑1 (MQ=255)
tATGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTg                                                                                                                                          <  1:980246/140‑1 (MQ=255)
tATGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTg                                                                                                                                          <  1:1693554/140‑1 (MQ=255)
  tGGCGGACATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAg                                                                                                                                        <  1:1452994/140‑1 (MQ=255)
  tGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAg                                                                                                                                        <  1:1024627/140‑1 (MQ=255)
  tGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAg                                                                                                                                        <  2:1270431/140‑1 (MQ=255)
  tGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAg                                                                                                                                        <  1:2547751/140‑1 (MQ=255)
  tGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAg                                                                                                                                        <  1:1283400/140‑1 (MQ=255)
       gataTCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAata                                                                                                                                   <  2:2372333/137‑1 (MQ=255)
       gAAATCGCTGTTTTCATCAGCAACAATGAGGAAGCGAGATAACTGGTTGATCGGCTGAACATTCCATTCCATTATATGAAAGGAAAAATATTCATCAGTGGGGATGTCGTTAAGTAGCAGCTGGTAGTGGTTGAGCAata                                                                                                                                   <  2:2790507/140‑1 (MQ=255)
       gAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAata                                                                                                                                   >  1:3107970/1‑140 (MQ=255)
           tCGCTGTTGTCTTCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATgag                                                                                                                               <  1:1428702/140‑1 (MQ=255)
                 ttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGAt                                                                                                                         <  1:627020/140‑1 (MQ=255)
                 ttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGAt                                                                                                                         <  1:553106/140‑1 (MQ=255)
                        caGCACCCATGAGGAAGCGAGACACCTGGTTGAACGGCTGAACATTCCATCCCATTATATGAAAGCACACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                  <  2:1826581/140‑1 (MQ=255)
                        caGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                  <  2:2623228/140‑1 (MQ=255)
                               cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           <  2:2243933/140‑1 (MQ=255)
                               cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           <  2:1146723/140‑1 (MQ=255)
                               cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           <  1:2445904/140‑1 (MQ=255)
                               cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTACATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGTAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           <  2:1772735/140‑1 (MQ=255)
                               cATGAGCCAGCGAGAGAACTGGTTGAACGGCTGACCATTCCATTCCATTATATGAAAGCAAACCAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTTGc                                                                                                           <  2:1905345/140‑1 (MQ=255)
                                 tGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCAc                                                                                                         <  1:1783228/140‑1 (MQ=255)
                                  gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                        <  1:1007610/140‑1 (MQ=255)
                                  gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                        <  2:1936329/140‑1 (MQ=255)
                                    tgAAGCGAGAGCACTGGTTGAACGGCTGAACATTCCATTCCATTATATGCAAGCAAACACACACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTGGCAAGCt                                                                                                      <  2:2091755/139‑1 (MQ=255)
                                    tgAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                      <  2:1975607/139‑1 (MQ=255)
                                    ggAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATCTGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                      <  1:2467774/140‑1 (MQ=255)
                                     gAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                  <  2:244366/127‑1 (MQ=255)
                                        gCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTatat                                                                                                  >  1:1927594/1‑140 (MQ=255)
                                         cGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                 <  1:1798542/140‑1 (MQ=255)
                                          gagataACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGc                                                                                                <  1:2315767/140‑1 (MQ=255)
                                           agagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCa                                                                                               <  1:1987015/140‑1 (MQ=255)
                                           agagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCa                                                                                               <  1:1240750/140‑1 (MQ=255)
                                             agaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                 >  2:2074360/1‑136 (MQ=255)
                                             agaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                 <  1:2074360/136‑1 (MQ=255)
                                                aCTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTTGCACGCTATATGcctgtct                                                                                          >  2:1472016/1‑134 (MQ=255)
                                                  tGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTa                                                                                        <  2:2854367/140‑1 (MQ=255)
                                                   ggTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTaa                                                                                       <  2:1626209/140‑1 (MQ=255)
                                                    gTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                      >  2:2756771/1‑140 (MQ=255)
                                                    gTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                      >  1:268502/1‑140 (MQ=255)
                                                    gTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                      >  1:1632820/1‑140 (MQ=255)
                                                    gTTGAACGGCTGAACATTCCATTCCAGTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                      >  1:1886499/1‑140 (MQ=255)
                                                         aCGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTg                                                                                 >  2:1430817/1‑140 (MQ=255)
                                                            gCTGAACATTCCATTCCATTATATGAAATCAAACAAAGACATAAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                              <  2:2712440/140‑1 (MQ=255)
                                                            gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                              <  2:555216/140‑1 (MQ=255)
                                                            ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                              >  2:2334833/2‑138 (MQ=255)
                                                            ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                              >  2:549998/2‑138 (MQ=255)
                                                            ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                              >  2:757249/2‑138 (MQ=255)
                                                                     tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                     <  2:2491/140‑1 (MQ=255)
                                                                     tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                     <  2:927932/140‑1 (MQ=255)
                                                                     tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCGTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                     >  2:667086/1‑140 (MQ=255)
                                                                     tccattccatTATATGAAAGCAAACAAAGACAACAGCGCGGAAGTCGAAAAGCAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                     <  2:2912971/140‑1 (MQ=255)
                                                                     tccattaaatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                     <  2:1869117/140‑1 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGTACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                   >  1:3087697/1‑140 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           >  1:83808/1‑100 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           <  2:1645626/100‑1 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                           >  1:1645626/1‑100 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                   >  1:513150/1‑140 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                   >  2:1666382/1‑140 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                   >  1:2082096/1‑140 (MQ=255)
                                                                       cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                   >  1:2418631/1‑140 (MQ=255)
                                                                            cattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATcc                                                              >  1:296230/1‑140 (MQ=255)
                                                                               tataTGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGaa                                                           >  1:1555221/1‑140 (MQ=255)
                                                                                atatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAAt                                                          <  2:21930/140‑1 (MQ=255)
                                                                                 tatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATc                                                         >  2:938144/1‑140 (MQ=255)
                                                                                  atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGCTTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                        <  1:1525708/140‑1 (MQ=255)
                                                                                  atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                        <  1:2021939/140‑1 (MQ=255)
                                                                                  atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                        <  1:2172032/140‑1 (MQ=255)
                                                                                  atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                        <  1:1114500/140‑1 (MQ=255)
                                                                                   tGAAAGCAAACAAATACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATCTGAGATTGATACGATCCTGCTTGCACGCGATATGCAGATCTTAACCCCTGACTATGGTTCGGCTCATACGAATCga                                                       >  2:2961754/1‑139 (MQ=255)
                                                                                   tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                       <  1:3012875/140‑1 (MQ=255)
                                                                                   tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                       <  1:369251/140‑1 (MQ=255)
                                                                                   tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                       <  1:1331345/140‑1 (MQ=255)
                                                                                     aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                     <  2:299986/140‑1 (MQ=255)
                                                                                     aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                     <  2:2027175/140‑1 (MQ=255)
                                                                                      aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                    >  1:1439155/1‑140 (MQ=255)
                                                                                      aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCATGACTTTGTTTCGGATCATCCGAATCGcatc                                                    >  2:1379644/1‑140 (MQ=255)
                                                                                           aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                               >  1:1932740/1‑140 (MQ=255)
                                                                                           aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                               >  1:943584/1‑140 (MQ=255)
                                                                                           aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                               >  1:2761780/1‑140 (MQ=255)
                                                                                               aaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                           <  2:1085112/140‑1 (MQ=255)
                                                                                                  aCATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcac                                        >  1:663293/1‑140 (MQ=255)
                                                                                                    aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                           <  1:574513/135‑1 (MQ=255)
                                                                                                    aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                           >  2:574513/1‑135 (MQ=255)
                                                                                                      cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCCTTCATATTCACCACt                                    >  1:1619757/1‑140 (MQ=255)
                                                                                                      cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                    <  2:3087697/140‑1 (MQ=255)
                                                                                                           cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGATATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                               >  2:1019766/1‑140 (MQ=255)
                                                                                                             gAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATGAATATTCACCACTCGTTcct                             >  2:1706840/1‑140 (MQ=255)
                                                                                                              aaGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAACACTGACTTTGTTTCGGCTCATCAGAATCGCATCATTAATATTAACCACTCGTTcctg                            >  2:2289394/1‑140 (MQ=255)
                                                                                                               aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                           <  1:3042931/140‑1 (MQ=255)
                                                                                                               aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                           <  1:2651494/140‑1 (MQ=255)
                                                                                                                         aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                 <  1:1666382/140‑1 (MQ=255)
                                                                                                                         aagCAGCTTGAACTGCTTGAGCAATATGAGAGTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                 <  2:1358242/140‑1 (MQ=255)
                                                                                                                           gcagcTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCg               >  2:880801/1‑140 (MQ=255)
                                                                                                                              gcTTGATCTTCTTGAGCAATTTGAGATTGATACGATAGTGCTTGCACGCTATTTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATGGCATCTTTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg            <  1:2119038/140‑1 (MQ=255)
                                                                                                                              gcTTGAACTGCTTGAGCAATATGAGATGGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg            <  1:1966797/140‑1 (MQ=255)
                                                                                                                                   aaCTGCTTGAGCTATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAAt       <  2:1736405/140‑1 (MQ=255)
                                                                                                                                   aaCTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCTCCTCTAGTTACTGCCTGCTTTTATCGGTGCGatt       <  2:458511/140‑3 (MQ=255)
                                                                                                                                        cTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                     <  1:1362251/105‑1 (MQ=255)
                                                                                                                                        cTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATa  >  2:701142/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
TATGGCGGAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATA  >  NZ_CP034943/1347501‑1347776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: