Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 266,842 T→C 8.3% C49C (TGT→TGC kdgD → 5‑dehydro‑4‑deoxyglucarate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943266,8420TC 8.3% 534.4 / 28.1 172C49C (TGT→TGCkdgD5‑dehydro‑4‑deoxyglucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (78/78);  new base C (7/7);  total (87/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 174 total aligned reads displayed.

TATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAT  >  NZ_CP034943/266704‑266972
                                                                                                                                          |                                                                                                                                  
tATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTtgtg                                                                                                                                   >  2:928418/1‑140 (MQ=255)
    agaAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTc                                                                                                                               <  1:166437/140‑1 (MQ=255)
      aaaaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTg                                                                                                                             >  2:2403526/1‑140 (MQ=255)
       aaaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTgg                                                                                                                            >  2:60101/1‑140 (MQ=255)
       aaaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTgg                                                                                                                            >  1:1911039/1‑140 (MQ=255)
        aaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGt                                                                                                                           >  2:2665848/1‑140 (MQ=255)
        aaGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGt                                                                                                                           >  2:239446/1‑140 (MQ=255)
          gCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGa                                                                                                                         >  1:60340/1‑140 (MQ=255)
                      aTCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAAtctct                                                                                                             <  1:672158/140‑1 (MQ=255)
                            ggTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCa                                                                                                       >  2:3020096/1‑140 (MQ=255)
                                ttCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaa                                                                                                   >  2:192315/1‑140 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagacgg                                                                                                  >  2:2667837/1‑137 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                                  >  2:489429/1‑138 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                                  >  2:2419981/1‑138 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                                  >  2:159442/1‑138 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                                  >  2:1230601/1‑138 (MQ=255)
                                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATATCTCAGCCagaagg                                                                                                  >  2:1473891/1‑138 (MQ=255)
                                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaag                                                                                                  >  2:1410098/1‑140 (MQ=255)
                                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaag                                                                                                  >  2:1610417/1‑140 (MQ=255)
                                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                     <  2:2868433/137‑1 (MQ=255)
                                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                     <  2:2815567/137‑1 (MQ=255)
                                   cgtttGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGGTTGGAAGCGATTTTTATCGCTTGTGGGTATGGTGAATTTCAATCTCTCAGCCagaaagaa                                                                                                <  2:1814133/137‑1 (MQ=255)
                                           ccTTTTAACTCACAAGGAACACTGGAGGAAGCAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTTGAAGCGATTTTTATCGCTTGTGTGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCa                                                                                        <  1:1619081/140‑1 (MQ=255)
                                           ccTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGTCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCa                                                                                        <  1:1506218/140‑1 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGTAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                             >  2:1565189/1‑140 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTTGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCATAAAGAATACGAGCAAATGGTGGacg                                                                             >  2:1183687/1‑138 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGACTACGAGCAAATGGTGGAAg                                                                             >  1:2926121/1‑140 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                             >  2:2646031/1‑140 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                             >  1:2736981/1‑140 (MQ=255)
                                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGACAGAAAGAATACGAGCAAATGGTGGAAg                                                                             >  1:1870285/1‑140 (MQ=255)
                                                       caAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGt                                                                            >  2:3088433/1‑140 (MQ=255)
                                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                      >  1:2828769/1‑129 (MQ=255)
                                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                      >  1:2333430/1‑129 (MQ=255)
                                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                           <  2:477498/140‑1 (MQ=255)
                                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                           <  2:2140385/140‑1 (MQ=255)
                                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                           >  1:1354903/1‑140 (MQ=255)
                                                        aaGGAAAAATTGATTATGTAGCTCTTTTTAAAAATATCGAGTTTTTGGTGGAATAAGTATTGTAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAATAATACGAGCAAATGTTGGAAGTc                                                                           <  2:2140593/140‑1 (MQ=255)
                                                                  ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                                 >  2:1650704/1‑140 (MQ=255)
                                                                  ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                                 >  1:2854714/1‑140 (MQ=255)
                                                                  ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTGGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                                 >  1:1328902/1‑140 (MQ=255)
                                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCTGCCAGAAAGAATACGAGCAAATGGTGGAAGTAGCACTCTCGGCAg                                                              >  2:2340399/1‑140 (MQ=255)
                                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                              >  1:1989628/1‑140 (MQ=255)
                                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGAGTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                              >  2:2345765/1‑140 (MQ=255)
                                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                             >  2:2490601/1‑140 (MQ=255)
                                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                             >  2:1208887/1‑140 (MQ=255)
                                                                         gaagCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCgg                                                          >  1:503408/1‑140 (MQ=255)
                                                                             ctctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCggagga                                                      <  2:500179/140‑1 (MQ=255)
                                                                              tctTTTTCAAAATATCGTGTTTTTTGTTGAAGTATGCTTTGAAGCTATTTTTATCGCTTGTTGTTATGGTGAATTTCAATCTCTCAGGCAGAAAGAATACTAGCAAATGGTGGTAGTAGCAATCTCGGCAGCCGTTGGaa                                                     <  2:1232784/140‑1 (MQ=255)
                                                                              tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                     <  2:855500/140‑1 (MQ=255)
                                                                              tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                     <  1:3020096/140‑1 (MQ=255)
                                                                              tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                     <  1:3018202/140‑1 (MQ=255)
                                                                                tttttCAAAATATCGAGTTTTGGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAg                                                   >  2:2326173/1‑140 (MQ=255)
                                                                                   ttCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGtt                                                <  2:2094139/140‑1 (MQ=255)
                                                                                    tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                               <  2:826888/140‑1 (MQ=255)
                                                                                    tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                               <  2:2002984/140‑1 (MQ=255)
                                                                                    tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                               <  2:2769879/140‑1 (MQ=255)
                                                                                     cAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGCGGAAAGGTTcc                                              >  2:2731497/1‑140 (MQ=255)
                                                                                     cAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTcc                                              >  2:3056195/1‑140 (MQ=255)
                                                                                      aaaaTATCTAGTTTTTGGTTGAAGAATGATTGTAAGCGATTTTTATCGATTGTTGGTCTGGTGAATTTAAATCTCTCTTCAAGATATAATACGAGCAATTGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCg                                             <  2:1027872/140‑1 (MQ=255)
                                                                                      aaaaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTTAATTTCAATCTCTCAGCCATAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCg                                             <  2:2434714/140‑1 (MQ=255)
                                                                                        aaTCTCGAGTTTTTGGTGGAAGAAGGCTTGGAAGAGATTTTTATCTCTTGTGGGTCTGTTTAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGCTTCCGGt                                           <  1:205764/140‑1 (MQ=255)
                                                                                        aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                           <  1:1240321/140‑1 (MQ=255)
                                                                                        aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                           <  1:2702991/140‑1 (MQ=255)
                                                                                            tCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCACATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTac                                       >  2:723396/1‑140 (MQ=255)
                                                                                            tCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTac                                       >  2:2674933/1‑140 (MQ=255)
                                                                                             ccagatgttGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                      <  2:295893/133‑1 (MQ=255)
                                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                      <  1:922382/140‑1 (MQ=255)
                                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                      <  2:1989628/140‑1 (MQ=255)
                                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                      <  1:3088433/140‑1 (MQ=255)
                                                                                                          gaagaaGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCACATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcgg                         >  2:131201/1‑140 (MQ=255)
                                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                        <  1:1937247/140‑1 (MQ=255)
                                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                        <  1:805667/140‑1 (MQ=255)
                                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                        <  1:2132067/140‑1 (MQ=255)
                                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTCGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                        <  1:1187738/140‑1 (MQ=255)
                                                                                                           aagaagGCGTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATGTCACTCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                        <  1:2140972/140‑1 (MQ=255)
                                                                                                              aagGCTTGGAAGCTATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAAc                     <  1:1697167/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGACTACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:2646031/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:122901/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:2997327/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:2665848/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:2418420/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                   <  1:239446/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                   <  1:1473891/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                   <  1:159442/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                   <  1:2471353/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                   <  1:2419981/140‑1 (MQ=255)
                                                                                                                ggCTTGGAAGCGAGAGTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                   <  1:2667837/140‑1 (MQ=255)
                                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa              <  1:417215/140‑1 (MQ=255)
                                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa              <  1:1410098/140‑1 (MQ=255)
                                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa              <  1:334129/140‑1 (MQ=255)
                                                                                                                       aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa            <  1:73922/140‑1 (MQ=255)
                                                                                                                       aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa            <  1:928418/140‑1 (MQ=255)
                                                                                                                          cGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCa         >  2:806572/1‑140 (MQ=255)
                                                                                                                           gATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCAc        >  1:2532838/1‑140 (MQ=255)
                                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                             <  1:1618773/86‑1 (MQ=255)
                                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt       >  1:2001635/1‑140 (MQ=255)
                                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt       >  1:1033954/1‑140 (MQ=255)
                                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt       >  1:1233536/1‑140 (MQ=255)
                                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt       >  1:429128/1‑140 (MQ=255)
                                                                                                                                 tATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAt  <  1:485771/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                  
TATGAGAAAAGCACCCGCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAT  >  NZ_CP034943/266704‑266972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: