Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,347,645 A→G 5.2% Q177Q (CAA→CAG purU → formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,347,6450AG 5.2% 625.6 / 16.8 193Q177Q (CAA→CAGpurUformyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base A (61/116);  new base G (5/5);  total (68/125)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 208 total aligned reads displayed.

GAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGG  >  NZ_CP034943/1347508‑1347784
                                                                                                                                         |                                                                                                                                           
gataTCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAata                                                                                                                                           <  2:2372333/137‑1 (MQ=255)
gAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAata                                                                                                                                           >  1:1296952/1‑140 (MQ=255)
gAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAata                                                                                                                                           <  2:2775782/140‑1 (MQ=255)
      gCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGAt                                                                                                                                     <  1:1981054/140‑1 (MQ=255)
         gttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGa                                                                                                                                  <  1:2592253/140‑1 (MQ=255)
          ttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGAt                                                                                                                                 <  1:2033727/140‑1 (MQ=255)
          ttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGAt                                                                                                                                 <  1:627020/140‑1 (MQ=255)
          ttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGAt                                                                                                                                 <  1:553106/140‑1 (MQ=255)
               atcaGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGAt                                                                                                                            >  1:1626209/1‑140 (MQ=255)
               atcaGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGAt                                                                                                                            >  2:678783/1‑140 (MQ=255)
                 caGCACCCATGAGGAAGCGAGACACCTGGTTGAACGGCTGAACATTCCATCCCATTATATGAAAGCACACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                          <  2:1826581/140‑1 (MQ=255)
                 caGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                          <  2:2623228/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  1:156928/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  2:1146723/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  1:2445904/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  2:2888060/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  1:2080624/140‑1 (MQ=255)
                        cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTACATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGTAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  2:1772735/140‑1 (MQ=255)
                        agacagcaaGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACCAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGAGTGATCCGATCGTGCTTGc                                                                                                                   <  1:1472016/133‑1 (MQ=255)
                         aTGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCa                                                                                                                  <  1:3053567/140‑1 (MQ=255)
                          tGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCAc                                                                                                                 <  1:1783228/140‑1 (MQ=255)
                           gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                <  2:1664274/140‑1 (MQ=255)
                           gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                <  1:3091076/140‑1 (MQ=255)
                           gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                <  2:1936811/140‑1 (MQ=255)
                           gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                <  1:1007610/140‑1 (MQ=255)
                           gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                <  1:1936711/140‑1 (MQ=255)
                             ggAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATCTGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                              <  1:2467774/140‑1 (MQ=255)
                             ggAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                              <  1:141056/140‑1 (MQ=255)
                             ggAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                              <  2:2729567/140‑1 (MQ=255)
                              gAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                          <  2:244366/127‑1 (MQ=255)
                                 gCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTatat                                                                                                          >  1:1927594/1‑140 (MQ=255)
                                  cGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                         <  1:1360766/140‑1 (MQ=255)
                                  cGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                         <  1:1798542/140‑1 (MQ=255)
                                   gagataACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGc                                                                                                        <  1:2315767/140‑1 (MQ=255)
                                   gagagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTACTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGc                                                                                                        <  1:1430099/140‑1 (MQ=255)
                                    agagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCa                                                                                                       <  1:1240750/140‑1 (MQ=255)
                                           tGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTa                                                                                                <  1:2717832/140‑1 (MQ=255)
                                             gTTGAACGGCTGAACATTCCATTCCAGTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                              >  1:1886499/1‑140 (MQ=255)
                                               tGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACCAACCCATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAccc                                                                                            <  1:267651/140‑1 (MQ=255)
                                                     gcggAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTGGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                      <  2:928229/137‑1 (MQ=255)
                                                     gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                      <  2:1979246/140‑1 (MQ=255)
                                                     gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                      <  2:555216/140‑1 (MQ=255)
                                                     ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                      >  2:549998/2‑138 (MQ=255)
                                                     ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                      >  2:2106899/2‑138 (MQ=255)
                                                     ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                      >  2:2334833/2‑138 (MQ=255)
                                                              tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                             <  2:2491/140‑1 (MQ=255)
                                                              tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                             >  2:1407120/1‑140 (MQ=255)
                                                              tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCGTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                             >  2:667086/1‑140 (MQ=255)
                                                              tccattaaatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                             <  2:1869117/140‑1 (MQ=255)
                                                                cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   <  2:83808/100‑1 (MQ=255)
                                                                cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                   >  1:1645626/1‑100 (MQ=255)
                                                                cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                           >  1:513150/1‑140 (MQ=255)
                                                                cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                           >  1:2418631/1‑140 (MQ=255)
                                                                cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                           >  2:1667944/1‑140 (MQ=255)
                                                                     cattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATcc                                                                      >  1:296230/1‑140 (MQ=255)
                                                                        tataTGAAAGCAAACAAAGACATCCGAGCGGTAGGCGAAAAGAAGCAGCTTGAACTGCTTGTGCACTGTGAGAGTGCTACGAGCGTGCTTGCACGGTAGATGCAGATCTTAACCCCTGACTTTGTGTCGGCTCATCCGag                                                                   >  1:2006591/1‑139 (MQ=255)
                                                                         atatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAAt                                                                  <  2:21975/140‑1 (MQ=255)
                                                                          tatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATc                                                                 >  2:1931162/1‑140 (MQ=255)
                                                                           atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                <  1:1114500/140‑1 (MQ=255)
                                                                           atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                <  1:2172032/140‑1 (MQ=255)
                                                                              aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                             <  2:889044/140‑1 (MQ=255)
                                                                              aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                             <  1:322119/140‑1 (MQ=255)
                                                                               aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                            >  1:3027087/1‑140 (MQ=255)
                                                                               aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                            >  1:1439155/1‑140 (MQ=255)
                                                                               aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                            >  1:431350/1‑140 (MQ=255)
                                                                                 gCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatcat                                                          <  2:296230/140‑1 (MQ=255)
                                                                                    aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                       >  1:2761780/1‑140 (MQ=255)
                                                                                    aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                       >  1:899772/1‑140 (MQ=255)
                                                                                        aaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                                   <  2:1085112/140‑1 (MQ=255)
                                                                                             aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                                   >  2:574513/1‑135 (MQ=255)
                                                                                             aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                                   <  1:574513/135‑1 (MQ=255)
                                                                                              tCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                             <  2:1296952/140‑1 (MQ=255)
                                                                                               cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                            <  2:2082096/140‑1 (MQ=255)
                                                                                               cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                            <  2:3087697/140‑1 (MQ=255)
                                                                                                 gagCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCg                                          >  2:1630410/1‑140 (MQ=255)
                                                                                                    cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                                       >  2:2029584/1‑140 (MQ=255)
                                                                                                    cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                                       >  2:2938829/1‑140 (MQ=255)
                                                                                                    cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGATATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                                       >  2:1019766/1‑140 (MQ=255)
                                                                                                       aaGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAACACTGACTTTGTTTCGGCTCATCAGAATCGCATCATTAATATTAACCACTCGTTcctg                                    >  2:2289394/1‑140 (MQ=255)
                                                                                                        aGTCGAAAAGAAGTAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                   <  1:1552511/140‑1 (MQ=255)
                                                                                                        aGTAGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                   <  2:2851512/140‑1 (MQ=255)
                                                                                                                  aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                         <  1:1667944/140‑1 (MQ=255)
                                                                                                                  aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGATTGCACGATATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                         <  2:753061/140‑1 (MQ=255)
                                                                                                                  aagCAGCTTGAACTGAGTGAGCAATATGAGATTGATCCGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTTACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                         <  2:1592964/140‑1 (MQ=255)
                                                                                                                       gcTTGATCTTCTTGAGCAATTTGAGATTGATACGATAGTGCTTGCACGCTATTTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATGGCATCTTTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                    <  1:2119038/140‑1 (MQ=255)
                                                                                                                       gcTTGAACTTCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                    <  2:2701374/140‑1 (MQ=255)
                                                                                                                       gcTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                    <  2:1463172/140‑1 (MQ=255)
                                                                                                                            aaCTGCTTGAGCTATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAAt               <  2:1736405/140‑1 (MQ=255)
                                                                                                                            aaCTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCTCCTCTAGTTACTGCCTGCTTTTATCGGTGCGatt               <  2:458511/140‑3 (MQ=255)
                                                                                                                                 cTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                             >  2:1362251/1‑105 (MQ=255)
                                                                                                                                    gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa       <  2:1633331/140‑1 (MQ=255)
                                                                                                                                    gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa       <  1:947532/140‑1 (MQ=255)
                                                                                                                                    gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa       <  2:1886499/140‑1 (MQ=255)
                                                                                                                                      gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc     >  2:3012894/1‑140 (MQ=255)
                                                                                                                                      gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc     <  1:1931162/140‑1 (MQ=255)
                                                                                                                                         atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg  >  1:2833723/1‑140 (MQ=255)
                                                                                                                                         atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg  <  1:771919/140‑1 (MQ=255)
                                                                                                                                         atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg  >  1:2571676/1‑140 (MQ=255)
                                                                                                                                         atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAAGCGCAGCATTATTATTCACCACTCGTTCCTGCCTGCTTTTAGCGGTGCGAATCCATACAAACggg  >  1:726008/1‑140 (MQ=255)
                                                                                                                                         atagGAGATTGATACGATCGTGCGTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg  <  1:160409/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GAAATCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGG  >  NZ_CP034943/1347508‑1347784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: