Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,347,648 T→C 5.2% Y178Y (TAT→TAC purU → formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,347,6480TC 5.2% 646.5 / 16.6 193Y178Y (TAT→TACpurUformyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base T (63/120);  new base C (5/5);  total (68/125)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 205 total aligned reads displayed.

TCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCT  >  NZ_CP034943/1347512‑1347786
                                                                                                                                        |                                                                                                                                          
tCGCTGTTGTCTTCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATgag                                                                                                                                         <  1:1428702/140‑1 (MQ=255)
     gttgtCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGa                                                                                                                                    <  1:2592253/140‑1 (MQ=255)
           atcaGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGAt                                                                                                                              >  2:678783/1‑140 (MQ=255)
           atcaGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGAt                                                                                                                              >  2:1715890/1‑140 (MQ=255)
                    cATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                     <  2:2602919/140‑1 (MQ=255)
                      tGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCAc                                                                                                                   <  1:1783228/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  1:1007610/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  2:1182517/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  2:1664274/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  1:57348/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  1:3091076/140‑1 (MQ=255)
                       gAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACg                                                                                                                  <  2:1936811/140‑1 (MQ=255)
                         ggAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                                <  2:1049680/140‑1 (MQ=255)
                         cgAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCt                                                                                                                <  1:2452425/139‑1 (MQ=255)
                          gAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                            >  1:244366/1‑127 (MQ=255)
                          gAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCg                                                                                                                            <  2:244366/127‑1 (MQ=255)
                             gCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTatat                                                                                                            >  1:1927594/1‑140 (MQ=255)
                              cGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                           <  1:1798542/140‑1 (MQ=255)
                                agagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCa                                                                                                         <  1:1240750/140‑1 (MQ=255)
                                agagaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCa                                                                                                         <  1:1987015/140‑1 (MQ=255)
                                  agaACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATg                                                                                                           <  1:2074360/136‑1 (MQ=255)
                                     aCTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTTGCACGCTATATGcctgtct                                                                                                    >  2:1472016/1‑134 (MQ=255)
                                       tGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTa                                                                                                  <  1:2717832/140‑1 (MQ=255)
                                       tGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTa                                                                                                  <  2:2854367/140‑1 (MQ=255)
                                        ggTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTaa                                                                                                 <  2:1626209/140‑1 (MQ=255)
                                         gTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                                >  2:2756771/1‑140 (MQ=255)
                                         gTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                                >  1:1632820/1‑140 (MQ=255)
                                         gTTGAACGGCTGAACATTCCATTCCAGTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAc                                                                                                >  1:1886499/1‑140 (MQ=255)
                                           tGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACCAACCCATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAAccc                                                                                              <  1:267651/140‑1 (MQ=255)
                                           tGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACCATCGTGCTTGAACGCTATATGCATAGATTAAccc                                                                                              >  2:2260406/1‑140 (MQ=255)
                                              aCGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTg                                                                                           >  2:1430817/1‑140 (MQ=255)
                                                 gcggAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCACTATGAGATTGATACGATCGTGCTGGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                        <  2:928229/137‑1 (MQ=255)
                                                 gCTGAACATTCCATTCCATTATATGAAATCAAACAAAGACATAAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                        <  2:2712440/140‑1 (MQ=255)
                                                 gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                        <  2:2352895/140‑1 (MQ=255)
                                                 gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                        <  2:555216/140‑1 (MQ=255)
                                                 gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                        <  2:1979246/140‑1 (MQ=255)
                                                 ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                        >  2:549998/2‑138 (MQ=255)
                                                 ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                        >  2:1137843/2‑138 (MQ=255)
                                                 ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                        >  2:2106899/2‑138 (MQ=255)
                                                   tGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACttt                                                                                      >  1:979770/1‑140 (MQ=255)
                                                          tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCGTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                               >  2:667086/1‑140 (MQ=255)
                                                          tccattccatTATATGAAAGCAAACAAAGACAACAGCGCGGAAGTCGAAAAGCAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                               <  2:2912971/140‑1 (MQ=255)
                                                          tccattaaatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                               <  2:1869117/140‑1 (MQ=255)
                                                            cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                     <  2:83808/100‑1 (MQ=255)
                                                            cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGc                                                                                                                     >  1:1645626/1‑100 (MQ=255)
                                                            cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                             >  2:1667944/1‑140 (MQ=255)
                                                            cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                             >  1:2082096/1‑140 (MQ=255)
                                                                 cattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATcc                                                                        >  1:296230/1‑140 (MQ=255)
                                                                    tataTGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGaa                                                                     >  1:1555221/1‑140 (MQ=255)
                                                                     atatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAAt                                                                    <  2:21975/140‑1 (MQ=255)
                                                                      tatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATc                                                                   >  2:1931162/1‑140 (MQ=255)
                                                                       atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGCTTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                  <  1:1525708/140‑1 (MQ=255)
                                                                       atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                  <  1:1114500/140‑1 (MQ=255)
                                                                       atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                  <  1:2021939/140‑1 (MQ=255)
                                                                       atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                  <  1:2172032/140‑1 (MQ=255)
                                                                        tGAAAGCAAACAAATACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATCTGAGATTGATACGATCCTGCTTGCACGCGATATGCAGATCTTAACCCCTGACTATGGTTCGGCTCATACGAATCga                                                                 >  2:2961754/1‑139 (MQ=255)
                                                                        tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                                 <  1:1331345/140‑1 (MQ=255)
                                                                        tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                                 <  1:3012875/140‑1 (MQ=255)
                                                                        tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                                 <  1:369251/140‑1 (MQ=255)
                                                                          aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                               <  2:889044/140‑1 (MQ=255)
                                                                          aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                               <  2:2292670/140‑1 (MQ=255)
                                                                           aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCATGACTTTGTTTCGGATCATCCGAATCGcatc                                                              >  2:1379644/1‑140 (MQ=255)
                                                                             gCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGACTCGcatcat                                                            >  2:1743022/1‑140 (MQ=255)
                                                                             gCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatcat                                                            >  2:1845748/1‑140 (MQ=255)
                                                                                aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                         >  1:899772/1‑140 (MQ=255)
                                                                                aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                         >  1:2761780/1‑140 (MQ=255)
                                                                                aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                         >  1:943584/1‑140 (MQ=255)
                                                                                         aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                                     >  2:574513/1‑135 (MQ=255)
                                                                                           cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCCTTCATATTCACCACt                                              >  1:1619757/1‑140 (MQ=255)
                                                                                           cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                              <  2:3087697/140‑1 (MQ=255)
                                                                                           cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                              <  2:2082096/140‑1 (MQ=255)
                                                                                             gagCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAAAATTAGATTGATAAGATCCTGCTTGCACGCTATATGCAGATCTTAACCCCAGACTTTGTTTAGGCTCATCCGAATCGCATCATTAATATTCACCACTCg                                            >  2:177576/1‑140 (MQ=255)
                                                                                                cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                                         >  2:2029584/1‑140 (MQ=255)
                                                                                                    aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                     <  1:1451563/140‑1 (MQ=255)
                                                                                                    aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                     <  1:3042931/140‑1 (MQ=255)
                                                                                                              aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                           <  1:1667944/140‑1 (MQ=255)
                                                                                                              aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGATTGCACGATATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                           <  2:753061/140‑1 (MQ=255)
                                                                                                              aagCAGCTTGAACTGCTTGAGCAATATGAGAGTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                           <  2:1358242/140‑1 (MQ=255)
                                                                                                              aagCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTAt                           <  1:549998/140‑1 (MQ=255)
                                                                                                              aagCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTAt                           <  1:2334833/140‑1 (MQ=255)
                                                                                                                   gcTTGATCTTCTTGAGCAATTTGAGATTGATACGATAGTGCTTGCACGCTATTTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATGGCATCTTTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                      <  1:2119038/140‑1 (MQ=255)
                                                                                                                   gcTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                      <  1:61827/140‑1 (MQ=255)
                                                                                                                   gcTTGAACTGCTTGAGCAATATGAGATGGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                      <  1:1966797/140‑1 (MQ=255)
                                                                                                                        aaCTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCTCCTCTAGTTACTGCCTGCTTTTATCGGTGCGatt                 <  2:458511/140‑3 (MQ=255)
                                                                                                                             cTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                               <  1:1362251/105‑1 (MQ=255)
                                                                                                                                gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa         <  2:1886499/140‑1 (MQ=255)
                                                                                                                                gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa         <  2:268502/140‑1 (MQ=255)
                                                                                                                                gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa         <  1:1430817/140‑1 (MQ=255)
                                                                                                                                gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa         <  2:1633331/140‑1 (MQ=255)
                                                                                                                                  gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc       <  1:1931162/140‑1 (MQ=255)
                                                                                                                                  gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc       >  2:3012894/1‑140 (MQ=255)
                                                                                                                                  gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCGGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc       <  2:2990943/140‑1 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg    >  1:1584410/1‑140 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg    >  1:2571676/1‑140 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg    >  1:2833723/1‑140 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg    <  1:771919/140‑1 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg    <  1:474610/140‑1 (MQ=255)
                                                                                                                                     atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAAGCGCAGCATTATTATTCACCACTCGTTCCTGCCTGCTTTTAGCGGTGCGAATCCATACAAACggg    >  1:726008/1‑140 (MQ=255)
                                                                                                                                       atGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCt  >  1:647593/1‑140 (MQ=255)
                                                                                                                                       atGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCt  >  1:2497004/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TCGCTGTTGTCATCAGCAACCATGAGGAAGCGAGAGAACTGGTTGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCT  >  NZ_CP034943/1347512‑1347786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: