Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,347,693 C→T 5.6% T193T (ACC→ACT purU → formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,347,6930CT 5.6% 702.8 / 20.8 212T193T (ACC→ACTpurUformyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  ref base C (106/94);  new base T (7/5);  total (113/99)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

Only 100 of 215 total aligned reads displayed.

TGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTATGTC  >  NZ_CP034943/1347555‑1347831
                                                                                                                                          |                                                                                                                                          
tGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACCATCGTGCTTGAACGCTATATGCATAGATTAAccc                                                                                                                                           >  2:2260406/1‑140 (MQ=255)
      gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                                                                     <  2:555216/140‑1 (MQ=255)
      gCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACt                                                                                                                                     <  2:1979246/140‑1 (MQ=255)
      ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                                                                     >  2:757249/2‑138 (MQ=255)
      ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                                                                     >  2:549998/2‑138 (MQ=255)
      ccTGAACATTCCATTCCACTATATGAAAGCGAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGatt                                                                                                                                     >  2:2334833/2‑138 (MQ=255)
        tGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACttt                                                                                                                                   >  1:979770/1‑140 (MQ=255)
            cattccattaCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGATGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCGATATGCAGATCTTAACCCCTGACTTTGttt                                                                                                                               <  2:368099/140‑1 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            <  2:927932/140‑1 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            <  2:468945/140‑1 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            <  2:2491/140‑1 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            <  2:1881074/140‑1 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            >  2:1407120/1‑140 (MQ=255)
               tccattccatTATATGAAAGCAAACAAAGACAACAGCGCGGAAGTCGAAAAGCAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCgg                                                                                                                            <  2:2912971/140‑1 (MQ=255)
                 cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                                                                          >  1:2506190/1‑140 (MQ=255)
                 cattccattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCt                                                                                                                          >  2:1666382/1‑140 (MQ=255)
                      cattATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATcc                                                                                                                     >  1:296230/1‑140 (MQ=255)
                         tataTGAAAGCAAACAAAGACATCCGAGCGGTAGGCGAAAAGAAGCAGCTTGAACTGCTTGTGCACTGTGAGAGTGCTACGAGCGTGCTTGCACGGTAGATGCAGATCTTAACCCCTGACTTTGTGTCGGCTCATCCGag                                                                                                                  >  1:2006591/1‑139 (MQ=255)
                           tatGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATc                                                                                                                >  2:938144/1‑140 (MQ=255)
                            atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                                                               <  1:1114500/140‑1 (MQ=255)
                            atGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCg                                                                                                               <  1:2172032/140‑1 (MQ=255)
                             tGAAAGCAAACAAATACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATCTGAGATTGATACGATCCTGCTTGCACGCGATATGCAGATCTTAACCCCTGACTATGGTTCGGCTCATACGAATCga                                                                                                              >  2:2961754/1‑139 (MQ=255)
                             tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                                                                              <  1:1331345/140‑1 (MQ=255)
                             tGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGc                                                                                                              <  1:369251/140‑1 (MQ=255)
                               aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                                                                            <  2:2292670/140‑1 (MQ=255)
                               aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                                                                            <  2:299986/140‑1 (MQ=255)
                               aaaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcat                                                                                                            <  1:322119/140‑1 (MQ=255)
                                aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                                                                           >  1:431350/1‑140 (MQ=255)
                                aaGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatc                                                                                                           >  1:3027087/1‑140 (MQ=255)
                                  gCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGACTCGcatcat                                                                                                         >  2:1743022/1‑140 (MQ=255)
                                  gCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGcatcat                                                                                                         >  2:1845748/1‑140 (MQ=255)
                                     aacaaaGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGATTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTaa                                                                                                      <  2:3023865/140‑1 (MQ=255)
                                            aCATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcac                                                                                               >  1:663293/1‑140 (MQ=255)
                                              aTCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATAtt                                                                                                  >  2:574513/1‑135 (MQ=255)
                                               tCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                                                                            <  2:1296952/140‑1 (MQ=255)
                                                cAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACt                                                                                           <  2:2418631/140‑1 (MQ=255)
                                                     cGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTc                                                                                      >  2:2029584/1‑140 (MQ=255)
                                                       gAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATGAATATTCACCACTCGTTcct                                                                                    >  2:1706840/1‑140 (MQ=255)
                                                         aGTCGAAAAGAAGTAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                                                                  <  1:1552511/140‑1 (MQ=255)
                                                         aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                                                                  <  1:3042931/140‑1 (MQ=255)
                                                         aGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTcctgc                                                                                  <  1:2651494/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                                                                        <  1:1666382/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGCTTGAGCAATATGAGAGTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                                                                        <  2:1358242/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTAt                                                                        <  1:549998/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTAt                                                                        <  1:1137843/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGCTGGAGCAGTACGATGTTGATGTGATCGTGCTCGCACGCTATATGCAGATTCTAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTAt                                                                        <  1:2106899/140‑1 (MQ=255)
                                                                   aagCAGCTTGAACTGAGTGAGCAATATGAGATTGATCCGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTTACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTAt                                                                        <  2:1592964/140‑1 (MQ=255)
                                                                     gcagcTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCg                                                                      >  2:880801/1‑140 (MQ=255)
                                                                        gcTTGAACTTCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                                                                   <  2:2701374/140‑1 (MQ=255)
                                                                        gcTTGAACTGCTTGAGCAATATGAGATGGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTg                                                                   <  1:1966797/140‑1 (MQ=255)
                                                                                  cTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTcaccac                                                                                            >  2:1362251/1‑105 (MQ=255)
                                                                                     gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa                                                      <  2:1632820/140‑1 (MQ=255)
                                                                                     gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa                                                      <  1:2756771/140‑1 (MQ=255)
                                                                                     gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa                                                      <  1:947532/140‑1 (MQ=255)
                                                                                     gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa                                                      <  2:268502/140‑1 (MQ=255)
                                                                                     gAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACaa                                                      <  1:1430817/140‑1 (MQ=255)
                                                                                       gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc                                                    <  1:938144/140‑1 (MQ=255)
                                                                                       gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc                                                    <  1:1931162/140‑1 (MQ=255)
                                                                                       gCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAAc                                                    <  2:677209/140‑1 (MQ=255)
                                                                                          atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg                                                 >  1:2571676/1‑140 (MQ=255)
                                                                                          atatGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg                                                 <  1:771919/140‑1 (MQ=255)
                                                                                          atagGAGATTGATACGATCGTGCGTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACggg                                                 <  1:160409/140‑1 (MQ=255)
                                                                                                gaTTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACg                                           >  1:1775092/1‑140 (MQ=255)
                                                                                                 aTTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGa                                          >  2:2821290/1‑140 (MQ=255)
                                                                                                    gATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAgcg                                       <  1:1584066/140‑1 (MQ=255)
                                                                                                    gATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAgcg                                       <  1:1243961/140‑1 (MQ=255)
                                                                                                    gATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGGTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAgcg                                       <  1:2494571/140‑1 (MQ=255)
                                                                                                     aTACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAgcgc                                      >  1:2847055/1‑140 (MQ=255)
                                                                                                          aTCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGt                                 >  2:208398/1‑140 (MQ=255)
                                                                                                           tCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTg                                >  2:2512535/1‑140 (MQ=255)
                                                                                                           tCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTg                                >  2:2080920/1‑140 (MQ=255)
                                                                                                                   gCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGTGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGAt                        <  1:816771/140‑1 (MQ=255)
                                                                                                                   gCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGTGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGAt                        <  1:2363757/140‑1 (MQ=255)
                                                                                                                   gCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGAt                        >  1:2798768/1‑140 (MQ=255)
                                                                                                                         tataTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGc                  >  1:203721/1‑140 (MQ=255)
                                                                                                                         tataTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGc                  >  1:2705864/1‑140 (MQ=255)
                                                                                                                         tataTGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGc                  >  1:1441194/1‑140 (MQ=255)
                                                                                                                           tatGCAGATCTTAACCCGTTACTTTGTTTCGGCTCATCCGTATCGCATCATTAATATTCACCACTCGTTGCTTGCTGCTTTTATGGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGa                <  1:703595/140‑1 (MQ=255)
                                                                                                                           tatGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGa                <  1:2194888/140‑1 (MQ=255)
                                                                                                                           tatGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGa                <  1:3068192/140‑1 (MQ=255)
                                                                                                                           tatGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGa                <  1:490199/140‑1 (MQ=255)
                                                                                                                                agattctAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTATCGGTGCGAATCCGTACAAACGGGCCTACGAGCGCGGCGTGAAACTGATCGGTGCGACAtct           >  2:1906599/7‑140 (MQ=255)
                                                                                                                                agattctAACTCCTGATTTTGTTTCGGCTCATCCGAATCGCATCATCAATATCCACCATTCATTCCTGCCAGCTTTTATCGGTGCGAATCCGTACAAACGGGCCTACGAGCGCGGCGTGAAACTGATCGGTGCGACAtct           >  2:630674/7‑140 (MQ=255)
                                                                                                                                 gATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTca                                                                                                <  2:2182575/54‑1 (MQ=255)
                                                                                                                                  aTCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCa         >  2:739506/1‑140 (MQ=255)
                                                                                                                                  aTCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCa         >  2:1459649/1‑140 (MQ=255)
                                                                                                                                  aTCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCa         >  2:1170945/1‑140 (MQ=255)
                                                                                                                                  aTCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCa         >  2:1073934/1‑140 (MQ=255)
                                                                                                                                   tCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCAc        >  1:854644/1‑140 (MQ=255)
                                                                                                                                   tCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCAc        >  1:1759054/1‑140 (MQ=255)
                                                                                                                                   tCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCGTACGAGCGCGGCGTGAAACTGATCGGAGCGACCTCTCAc        >  1:1672732/1‑140 (MQ=255)
                                                                                                                                    cTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACt       >  1:1852591/1‑140 (MQ=255)
                                                                                                                                     ttAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTa      >  2:1800786/1‑140 (MQ=255)
                                                                                                                                     ttAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTa      <  1:2772351/140‑1 (MQ=255)
                                                                                                                                     ttAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTa      >  1:2444879/1‑140 (MQ=255)
                                                                                                                                     ttAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGAGTGAAACTGATCGGAGCGACATCTCACTa      >  2:2973834/1‑140 (MQ=255)
                                                                                                                                       aaCCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCCGTAATATGCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAAGCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACCTCTGACtgtg    >  1:631178/1‑140 (MQ=255)
                                                                                                                                       aaCCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTATg    >  1:1005121/1‑140 (MQ=255)
                                                                                                                                        aCCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTATGt   <  1:678783/140‑1 (MQ=255)
                                                                                                                                        aCCCCTGACTTTGTTTCGGATCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTATGt   <  1:600966/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TGAACGGCTGAACATTCCATTCCATTATATGAAAGCAAACAAAGACATCAGAGCGGAAGTCGAAAAGAAGCAGCTTGAACTGCTTGAGCAATATGAGATTGATACGATCGTGCTTGCACGCTATATGCAGATCTTAACCCCTGACTTTGTTTCGGCTCATCCGAATCGCATCATTAATATTCACCACTCGTTCCTGCCTGCTTTTATCGGTGCGAATCCATACAAACGGGCTTACGAGCGCGGCGTGAAACTGATCGGAGCGACATCTCACTATGTC  >  NZ_CP034943/1347555‑1347831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: