Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,496,370 T→C 6.2% D245D (GAT→GAC pdhC → pyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,496,3700TC 6.2% 582.1 / 18.4 176D245D (GAT→GACpdhCpyruvate dehydrogenase complex dihydrolipoyllysine‑residue acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (78/87);  new base C (4/7);  total (82/94)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

AACCGGCTGCTGCACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAACACGTCAATT  >  NZ_CP034943/1496231‑1496508
                                                                                                                                           |                                                                                                                                          
aaCCGGCTGCTGCACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGAt                                                                                                                                            <  2:2297614/140‑1 (MQ=255)
     gctgctGCACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAAc                                                                                                                                       >  1:2240825/1‑140 (MQ=255)
       tgctgcACCAGCTCCAGAGGGCGCATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCAAAAGCGATGGTTAACTCTAAACACACTGCTCCTCACGTAACGTTAATGGACGAAGTGGACGTAACaa                                                                                                                                     >  1:281217/1‑140 (MQ=255)
       tgctgcACCAGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCAAAAGCGATGGTTAACTCTAAACACACTGCTCCTCACGTAACGTTAATGGACGAAGTGGACGTAACaa                                                                                                                                     >  1:1275633/1‑140 (MQ=255)
        gctgcACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACaaa                                                                                                                                    <  2:1655444/140‑1 (MQ=255)
          tgcACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAAcc                                                                                                                                  <  1:1501297/140‑1 (MQ=255)
          tgcACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAAcc                                                                                                                                  <  2:219172/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTACACACACAGCTCCTCACGGAACGGTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:51272/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:1538367/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  1:977121/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:250956/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  1:348517/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  1:2712270/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:2734379/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  1:1868826/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  1:2017139/140‑1 (MQ=255)
                 gCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTACTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:2772567/140‑1 (MQ=255)
                   tCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGcac                                                                                                                         <  1:2470757/140‑1 (MQ=255)
                   tCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGcac                                                                                                                         <  1:2395909/140‑1 (MQ=255)
                    ccAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTCAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGcaca                                                                                                                        >  2:1597259/1‑140 (MQ=255)
                           gCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTaaa                                                                                                                 <  2:3521/140‑1 (MQ=255)
                           gCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTaaa                                                                                                                 <  2:2619088/140‑1 (MQ=255)
                           gCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTaaa                                                                                                                 <  1:596563/140‑1 (MQ=255)
                           gCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTaaa                                                                                                                 <  1:2422592/140‑1 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGTTATCCTTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACTTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAAc                                                                                                                <  2:216071/140‑1 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTCAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAAc                                                                                                                <  1:3010300/140‑1 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCACTTGCAAAAGCGATGGTTAACTCTAAACACACTGCTCCTCACGTAACGTTAATGGACGAAGTGGACGTAACAAACCTTGTTGCACATCGTAAAc                                                                                                                >  2:1374199/1‑140 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:1345960/129‑1 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:496844/129‑1 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAAc                                                                                                                >  1:1713656/1‑140 (MQ=255)
                            cGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAAc                                                                                                                <  1:2263671/140‑1 (MQ=255)
                               aTTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGt                                                                                                             >  1:1682758/1‑140 (MQ=255)
                               aTTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGt                                                                                                             >  2:1425130/1‑140 (MQ=255)
                               aTTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGAACATCGTAAACAGt                                                                                                             >  2:1056380/1‑140 (MQ=255)
                                ttCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGtt                                                                                                            <  1:2145999/140‑1 (MQ=255)
                                ttCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGtt                                                                                                            <  1:2938018/140‑1 (MQ=255)
                                     aGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAAc                                                                                                       >  1:308010/1‑140 (MQ=255)
                                              cgAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTtgctg                                                                                              <  2:2936711/140‑1 (MQ=255)
                                              cgAAAAAATGAGCGGTATCCGCAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAAAGTTAATGGACGAAGTTGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTtgctg                                                                                              <  2:2842004/140‑1 (MQ=255)
                                                             taacattAAACCAATTCCGAAAGAGATTTTTAACTAAAAAAAAAAATCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTTTTGCACATCGTAAAAAGTTCAAACAGGTTGCTGCTGATCAAGGAATCa                                                                               >  1:2200846/7‑140 (MQ=255)
                                                             tATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCa                                                                               <  1:1251580/140‑1 (MQ=255)
                                                               tCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAg                                                                             <  1:2553539/140‑1 (MQ=255)
                                                               tCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAg                                                                             >  2:1348582/1‑140 (MQ=255)
                                                                        cAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGacttac                                                                    <  1:2680942/140‑1 (MQ=255)
                                                                          aTTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTACTGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGacttagtt                                                                  >  1:1184607/1‑140 (MQ=255)
                                                                          aTTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGacttactt                                                                  >  1:2788996/1‑140 (MQ=255)
                                                                           ttGCGAACGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGacttacttc                                                                 >  2:701003/1‑139 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           >  1:347960/1‑82 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGc                                                                                                                           <  2:347960/82‑1 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGacttac                                                                    <  1:274010/137‑1 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTg                                                                 >  1:1335492/1‑140 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTg                                                                 >  1:765468/1‑140 (MQ=255)
                                                                           ttGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTg                                                                 >  1:1008928/1‑140 (MQ=255)
                                                                              caaaaGCGATGGTTAACTCTAAACACACTGCTCCTCACGTAACGTTAATGGACGAAGTGGACGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCt                                                              <  1:1374199/138‑1 (MQ=255)
                                                                                 aaGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGTAATCAAGCTGACTTACTTGCCTTAc                                                           <  2:1530955/140‑1 (MQ=255)
                                                                                 aaGAGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTAc                                                           <  2:2870330/140‑1 (MQ=255)
                                                                                  aGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGt                                                                                                   >  1:2917247/1‑99 (MQ=255)
                                                                                  aGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGt                                                                                                   <  2:2917247/99‑1 (MQ=255)
                                                                                  aGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACg                                                          <  2:2052053/140‑1 (MQ=255)
                                                                                        ggTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaa                                                    <  1:424601/140‑1 (MQ=255)
                                                                                         gTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaa                                                   <  2:1682758/140‑1 (MQ=255)
                                                                                         gTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaa                                                   <  2:155597/140‑1 (MQ=255)
                                                                                         gTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaa                                                   <  2:2205951/140‑1 (MQ=255)
                                                                                          ttAACTCTAAACACACAGCGCCTCACGTAACGTTAATGGCCGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaaa                                                  <  2:965058/140‑1 (MQ=255)
                                                                                                   aaCACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCt                                                                           <  2:1174662/106‑1 (MQ=255)
                                                                                                   aaCACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCt                                                                           >  1:1650126/1‑106 (MQ=255)
                                                                                                   aaCACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACa                                         >  2:2708662/1‑140 (MQ=255)
                                                                                                   aaCACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGGTGCTGCTGATCAAGTAATCAAGCTGACTTACTTGCCTTACGGTGTGAAAGCTCTTAct                                         >  2:2202341/1‑139 (MQ=255)
                                                                                                        acaGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaaa                                                  >  2:1744632/1‑126 (MQ=255)
                                                                                                        acaGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTaaaa                                                  <  1:1746459/126‑1 (MQ=255)
                                                                                                        acaGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGc                                    >  1:1167791/1‑140 (MQ=255)
                                                                                                        acaGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGc                                    >  1:1461291/1‑140 (MQ=255)
                                                                                                         caGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCa                                   >  2:217826/1‑140 (MQ=255)
                                                                                                         caGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCa                                   >  2:472131/1‑140 (MQ=255)
                                                                                                           gCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACt                                 >  1:888131/1‑140 (MQ=255)
                                                                                                            ctcctcACGTAACGTTAATGGACGAAGTGGACGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTg                                <  2:1622055/140‑1 (MQ=255)
                                                                                                               ctcACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaa                             >  1:1222814/1‑140 (MQ=255)
                                                                                                               ctcACGTAACGTTAATGGACGAAGTGGACGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaa                             <  1:1097416/140‑1 (MQ=255)
                                                                                                                  aCGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCACGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaaaaa                          >  2:1957251/1‑140 (MQ=255)
                                                                                                                  aCGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaaaaa                          >  1:693557/1‑140 (MQ=255)
                                                                                                                  aCGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaaaaa                          >  1:455198/1‑140 (MQ=255)
                                                                                                                  aCGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGaaaaaa                          >  2:2968619/1‑140 (MQ=255)
                                                                                                                      aaCGTTAATGGCCGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGATGCTGATCAAGGAATCAATCTGACTTACTTGCATTACGTTGTAAAAGCTCTTACATATGCACTGAAAAAAttac                      >  2:2040463/1‑138 (MQ=255)
                                                                                                                      aaCGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGGCTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTcc                      <  2:2240825/140‑1 (MQ=255)
                                                                                                                      aaCGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTcc                      <  2:1192248/140‑1 (MQ=255)
                                                                                                                      aaCGTTAATGGACGAAGTGGATGTAACAAACCTTGGTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTcc                      <  2:2380471/140‑1 (MQ=255)
                                                                                                                       aCGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTccc                     >  1:2293122/1‑140 (MQ=255)
                                                                                                                         gTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTg                   >  2:1836784/1‑140 (MQ=255)
                                                                                                                         gTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTg                   >  2:426355/1‑140 (MQ=255)
                                                                                                                         gTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTg                   >  2:636674/1‑140 (MQ=255)
                                                                                                                         gTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTg                   >  2:1244857/1‑140 (MQ=255)
                                                                                                                           tAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGtt                 >  1:2059255/1‑140 (MQ=255)
                                                                                                                                gACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTaaa            <  1:2344588/140‑1 (MQ=255)
                                                                                                                                gACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTaaa            <  1:2426517/140‑1 (MQ=255)
                                                                                                                                gACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTaaa            <  2:2059255/140‑1 (MQ=255)
                                                                                                                                  cGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAaca          >  1:1137536/1‑140 (MQ=255)
                                                                                                                                   gAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAacac         >  2:418448/1‑140 (MQ=255)
                                                                                                                                   gAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAacac         <  2:1222814/140‑1 (MQ=255)
                                                                                                                                     aGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAACACGt       >  2:3031117/1‑140 (MQ=255)
                                                                                                                                          aTGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAACACGTCAAtt  <  1:2202444/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AACCGGCTGCTGCACCGGCTCCAGAGGGCGAATTCCCAGAAACACGCGAAAAAATGAGCGGTATCCGTAAAGCAATTGCGAAAGCGATGGTTAACTCTAAACACACAGCTCCTCACGTAACGTTAATGGACGAAGTGGATGTAACAAACCTTGTTGCACATCGTAAACAGTTCAAACAGGTTGCTGCTGATCAAGGAATCAAGCTGACTTACTTGCCTTACGTTGTAAAAGCTCTTACATCTGCACTGAAAAAATTCCCTGTTTTAAACACGTCAATT  >  NZ_CP034943/1496231‑1496508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: