Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,733,974 C→G 5.9% V80V (GTC→GTG EO946_RS09000 → serine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,733,9740CG 5.9% 321.0 / 8.2 101V80V (GTC→GTGEO946_RS09000serine hydrolase
Reads supporting (aligned to +/- strand):  ref base C (54/41);  new base G (3/3);  total (57/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 101 total aligned reads displayed.

CGATTTGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCAAAATAATCCA  >  NZ_CP034943/1733838‑1734112
                                                                                                                                        |                                                                                                                                          
cGATTTGGAATGCTCTTCATCGAAATAGTGAAGAAAACGAACCATCTCAAGAAACTGCAGCGGTTTCAAATACCGATCAGAAAAAAGAGGTCAAAAAGAAAACTGCCAAAAAATCAGAGGAACAAATCAAAACAGTGGat                                                                                                                                         >  1:384758/1‑139 (MQ=255)
cGATTTGGAATGCTCTTCATCGAAATAGTGAAGAAAACGAACCATCTCAAGAAACTGCAGCGGTTTCAAATACCGATCAGAAAAAAGAGGTCAAAAAGAAAACTGCCAAAAAATCAGAGGAACAAATCAAAACAGTGGat                                                                                                                                         >  1:2562488/1‑139 (MQ=255)
cGATTTGGAATGCTCTTCATCGAAATAGTGAAGAAAACGAACCATCTCAAGAAACTGCAGCGGTTTCAAATACCGAGCAGAAAAAAGAGGTCAAAAAGAAAACTGCCAAAAAATCAGAGGACCAAATCAAAACAGTGGat                                                                                                                                         >  1:2117866/1‑139 (MQ=255)
     tGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCCAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTaa                                                                                                                                    >  1:656622/1‑140 (MQ=255)
     tGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTaa                                                                                                                                    >  2:2274594/1‑140 (MQ=255)
     tGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTaa                                                                                                                                    >  1:830020/1‑140 (MQ=255)
     tGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTaa                                                                                                                                    >  1:421176/1‑140 (MQ=255)
     tGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTaa                                                                                                                                    >  1:1985862/1‑140 (MQ=255)
                 cATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGACCAAATCAAAACAGTCGACCGTAATCAAAAAATCAg                                                                                                                        >  1:2876117/1‑140 (MQ=255)
                 cATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAg                                                                                                                        >  1:1890862/1‑140 (MQ=255)
                 cATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAACAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATAAg                                                                                                                        >  1:1782597/1‑140 (MQ=255)
                       aaTAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAatta                                                                                                                  >  2:1495446/1‑140 (MQ=255)
                                  gaACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAgaga                                                                                                       <  2:421176/140‑1 (MQ=255)
                                  gaACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAgaga                                                                                                       <  2:1985862/140‑1 (MQ=255)
                                   aaCGATCCATCTCAATAAACTGCAGCAGTTTCAGATAAACGCCAGAAAAAACAATTCAAAAAGAAAAACGCCAAAAAAACACACTAACAAAACAAAACAGTCGACACAAATCAAAAAATCATCAATTATTAaaaaaataa                                                                                                      >  2:2519959/1‑135 (MQ=255)
                                   aaCGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGAt                                                                                                      >  2:81730/1‑140 (MQ=255)
                                   aaCGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGAt                                                                                                      >  1:1855850/1‑140 (MQ=255)
                                   aaCGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGAt                                                                                                      >  1:2132488/1‑140 (MQ=255)
                                     cGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCg                                                                                                    >  1:2095382/1‑140 (MQ=255)
                                            tctcAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAg                                                                                             <  2:2817011/140‑1 (MQ=255)
                                            tctcAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAg                                                                                             <  1:81730/140‑1 (MQ=255)
                                            tctcAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAg                                                                                             <  2:322304/140‑1 (MQ=255)
                                            tctcAAGAAACTGCAGCACTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAACCCGCCAAAAAAACAGAGGAACAAAACAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAg                                                                                             <  2:2438254/140‑1 (MQ=255)
                                             ctcAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGc                                                                                            >  1:491284/1‑140 (MQ=255)
                                                 aGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTa                                                                                        <  2:2193102/140‑1 (MQ=255)
                                                 aGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTa                                                                                        <  2:2394851/140‑1 (MQ=255)
                                                    aaCTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGCCCGTACTCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAg                                                                                     >  2:2713868/1‑140 (MQ=255)
                                                     aCTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACACATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGc                                                                                    >  1:2061804/1‑140 (MQ=255)
                                                     aCTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGc                                                                                    >  1:2318895/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCCAAAAGAAAACCGCCACAAAAACAGAGGCACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:2955709/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:1229207/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:1134434/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:588962/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:405264/1‑140 (MQ=255)
                                                      cTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGcc                                                                                   >  2:2867243/1‑140 (MQ=255)
                                                       tGCAGCAGTTTCAGATCACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCCAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCa                                                                                  >  2:1356858/1‑140 (MQ=255)
                                                       tGCAGCAGTTTCAGATAGCGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCa                                                                                  >  2:450321/1‑140 (MQ=255)
                                                       tGCAGCAGTTTCAGATAACGGCCAGAAAATAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCa                                                                                  >  2:2022489/1‑140 (MQ=255)
                                                       tGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAACACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCa                                                                                  >  2:305509/1‑140 (MQ=255)
                                                       tGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTCCAGCCa                                                                                  >  2:1959735/1‑140 (MQ=255)
                                                          agcagTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGa                                                                               >  1:2941787/1‑140 (MQ=255)
                                                          agcagTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGa                                                                               >  2:925934/1‑140 (MQ=255)
                                                          agcagTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGa                                                                               <  2:2444658/140‑1 (MQ=255)
                                                              gTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCaa                                                                                                                     >  1:2403593/1‑98 (MQ=255)
                                                              gTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCaa                                                                                                                     <  2:2403593/98‑1 (MQ=255)
                                                              gTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGt                                                                           >  2:1089007/1‑140 (MQ=255)
                                                                 tCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAg                                                                        >  1:2072794/1‑140 (MQ=255)
                                                                 tCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAg                                                                        >  1:208696/1‑140 (MQ=255)
                                                                 tCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAg                                                                        >  1:455760/1‑140 (MQ=255)
                                                                 tCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAg                                                                        >  1:2734624/1‑140 (MQ=255)
                                                                 tCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAg                                                                        >  2:1025179/1‑140 (MQ=255)
                                                                        aCGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGga                                                                 <  1:1025179/140‑1 (MQ=255)
                                                                         cGGCCAGAAAATAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGACCAAATCAAAACGGTCGACCGTAATCAAAAAATCAGCAATTATTTAAATGTGATCGGTTTCAGCGGGACTGCCCTGATTGTCAGACATGgag                                                                >  1:2194945/1‑140 (MQ=255)
                                                                         cGGCCAGAAAAAAGAAGTCAAAAATAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCATCAATTAATTAAAAGAGATCGGTTTCAGCGGTACACCCATGATTGTCAGAAATGgag                                                                >  2:804316/1‑140 (MQ=255)
                                                                         cGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGgag                                                                >  2:1021678/1‑140 (MQ=255)
                                                                         cGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGAACGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGgag                                                                >  2:1453108/1‑140 (MQ=255)
                                                                               gAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATct                                                          >  2:1423148/1‑139 (MQ=255)
                                                                                 aaaaaGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTa                                                        <  1:887873/140‑1 (MQ=255)
                                                                                  aaaaGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTaa                                                       <  2:312678/140‑1 (MQ=255)
                                                                                  aaaaGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTaa                                                       <  2:2614586/140‑1 (MQ=255)
                                                                                  aaaaGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTaa                                                       <  2:1690940/140‑1 (MQ=255)
                                                                                  aaaaGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGACGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTaa                                                       <  2:2615192/140‑1 (MQ=255)
                                                                                        agTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAACTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATa                                                 <  1:2619174/140‑1 (MQ=255)
                                                                                        agTCAAAAACAAAACCGCCAAAAACACAGACCAACAAATCAACCCAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATa                                                 <  1:2617112/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCTCCAAAACAACAGACGAACAAATCCACAGATTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGCTCGGTTTCAGCGGTTCAGGCATGATTTTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  1:804316/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACCGAGGAACAACTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:1898431/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGTAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:1570607/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAACAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:2962887/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGTTTGTAAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:600493/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:2275969/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:2387764/140‑1 (MQ=255)
                                                                                             aaaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATAAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGt                                            <  2:2451467/140‑1 (MQ=255)
                                                                                              aaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGtt                                           >  2:1225026/1‑140 (MQ=255)
                                                                                              aaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGtt                                           >  2:1129482/1‑140 (MQ=255)
                                                                                              aaaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGtt                                           >  2:1129404/1‑140 (MQ=255)
                                                                                               aaGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGttt                                          <  1:1486757/140‑1 (MQ=255)
                                                                                                    aaCCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGtt                                     >  2:181169/1‑140 (MQ=255)
                                                                                                    aaCCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTGTGGtt                                     >  2:1378554/1‑140 (MQ=255)
                                                                                                       cGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATg                                  <  1:925934/140‑1 (MQ=255)
                                                                                                        gCCAAAAAAACAGACGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGc                                 <  1:1779972/140‑1 (MQ=255)
                                                                                                          cAAAAAATCAGAGGAACAAATCAAAACAGTGGATCGAAACCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGAACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGCTATGctg                               <  2:2562488/140‑3 (MQ=255)
                                                                                                          cAAAAAATCAGAGGAACAAATCAAAACAGTGGATCGAAACCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGAACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGCTATGctg                               <  2:2117866/140‑3 (MQ=255)
                                                                                                          cAAAAAATCAGAGGAACAAATCAAAACAGTGGATCGAAACCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGAACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGCTATGctg                               <  2:384758/140‑3 (MQ=255)
                                                                                                           aaaaaaaCAGAGGAACAAATCAACACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGa                              <  1:305509/140‑1 (MQ=255)
                                                                                                           aaaaaaaCAGAGGAACAAATCAACACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACACCCATTATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGa                              <  1:1453108/140‑1 (MQ=255)
                                                                                                           aaaaaaaCAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGa                              <  1:2022489/140‑1 (MQ=255)
                                                                                                           aaaaaaaCAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGa                              <  1:1021678/140‑1 (MQ=255)
                                                                                                           aaaaaaaCAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGa                              <  1:450321/140‑1 (MQ=255)
                                                                                                                     agGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGCTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAACTAAAGGTTTTGGTTATGCAGACCGTAAACAt                    <  2:69636/140‑1 (MQ=255)
                                                                                                                           aaaTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACAtt              <  1:20546/140‑1 (MQ=255)
                                                                                                                           aaaTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACAtt              <  1:2077954/140‑1 (MQ=255)
                                                                                                                           aaaTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACAtt              <  1:2409652/140‑1 (MQ=255)
                                                                                                                           aaaTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACAtt              <  1:119178/140‑1 (MQ=255)
                                                                                                                           aaaTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACAtt              <  1:709910/140‑1 (MQ=255)
                                                                                                                             aTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCa            >  1:1930844/1‑140 (MQ=255)
                                                                                                                             aTCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCa            >  1:296857/1‑140 (MQ=255)
                                                                                                                                 aaaCAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCAAaat        <  2:514622/140‑1 (MQ=255)
                                                                                                                                  aaCAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCAAaata       >  1:999693/1‑140 (MQ=255)
                                                                                                                                  aaCAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCAAaata       >  1:796111/1‑140 (MQ=255)
                                                                                                                                       tCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACAACACATTCAAAATAATCCa  >  1:1127021/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CGATTTGGAATGCGCTTCATCGAAATAGTGAAGAGAACGAGCCATCTCAAGAAACTGCAGCAGTTTCAGATAACGGCCAGAAAAAAGAAGTCAAAAAGAAAACCGCCAAAAAAACAGAGGAACAAATCAAAACAGTCGACCGTAATCAAAAAATCAGCAATTATTTAAAAGAGATCGGTTTCAGCGGTACAGCCATGATTGTCAGAAATGGAGAAATCGTAACAAATAAAGGTTTTGGTTATGCAGACCGTAAACATCACATTCAAAATAATCCA  >  NZ_CP034943/1733838‑1734112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: