Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,789,771 C→A 5.8% P4386T (CCT→ACT)  EO946_RS09130 → type I polyketide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,789,7710CA 5.8% 422.1 / 6.8 154P4386T (CCT→ACT) EO946_RS09130type I polyketide synthase
Reads supporting (aligned to +/- strand):  ref base C (64/78);  new base A (9/3);  total (73/81)
Fisher's exact test for biased strand distribution p-value = 6.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CGAAGACGGAGATTCCATATATGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAACCTAACA  >  NZ_CP034943/1789632‑1789906
                                                                                                                                           |                                                                                                                                       
cGAAGACGGAGATTCCATATATGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGc                                                                                                                                         <  1:312743/140‑1 (MQ=255)
        gagaTTCCATATATGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAagag                                                                                                                                 <  2:642929/140‑1 (MQ=255)
                   tatGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTAtct                                                                                                                      >  1:2687814/1‑140 (MQ=255)
                   tatGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTAtct                                                                                                                      >  1:457543/1‑140 (MQ=255)
                     tGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCg                                                                                                                    >  1:1403456/1‑140 (MQ=255)
                     tGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCg                                                                                                                    >  1:2176216/1‑140 (MQ=255)
                            attaAAGCCTCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAATGATGACATGCAGACAGCGCTTCAAAAAAGCGGCAAAAATCCAGAACAAATCAGTTATCACCAAGCGaa                                                                                                             >  2:3051182/1‑140 (MQ=255)
                            attaAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAag                                                                                                                                   <  1:1055782/118‑1 (MQ=255)
                            attaAAGCCGCTGCTATCAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGaa                                                                                                             <  2:2699177/140‑1 (MQ=255)
                             ttaAAGCCGCTGCTATTAACAATCACGGAAGAACAGCATCCCCATCTTCAACGAATCTCCAGGCACAAAAGGATGTCATGCACACAGCGCTTGAAAAAAGCCGCCAAAACCCAGACTAGAACAAATAACTCGaaaacact                                                                                                            >  2:1785141/1‑134 (MQ=255)
                                aaGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGc                                                                                                         <  1:453872/140‑1 (MQ=255)
                                  gCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCTATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAATCGAATGGCTc                                                                                                       >  1:1591393/1‑140 (MQ=255)
                                  gCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTc                                                                                                       >  1:51021/1‑140 (MQ=255)
                                      ctgctATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGa                                                                                                   >  1:460683/1‑140 (MQ=255)
                                      ctgctATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTCAAGAGATAAGTTATCACGAAGCGAAAGGCTCGGGa                                                                                                   >  2:171685/1‑140 (MQ=255)
                                           aTTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGc                                                                                              <  1:1596470/140‑1 (MQ=255)
                                                cAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTaa                                                                                         <  2:2171662/140‑1 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCCCAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCGGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:2507799/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGACAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:1759004/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:1891372/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:653164/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:2640449/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:1452078/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:1305896/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:2729044/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGAGAATGGCTCGGGATCGGCAGTAACAg                                                                                      >  1:3091264/1‑140 (MQ=255)
                                                   tGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAACCGGCAAAAACAAAGAAGAGATAAGTTATATCGAAGAGAATGGATAGGGATCGGCAGTAACAg                                                                                      >  2:2659452/1‑140 (MQ=255)
                                                    gACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGa                                                                                     >  1:1980302/1‑140 (MQ=255)
                                                          agaaCAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGacttact                                                                               <  1:2972703/140‑1 (MQ=255)
                                                                   ggCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGaa                                                                      >  1:1081198/1‑140 (MQ=255)
                                                                   ggCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGaa                                                                      >  2:148926/1‑140 (MQ=255)
                                                                   ggCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGaa                                                                      <  2:1241657/140‑1 (MQ=255)
                                                                     cccATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAgg                                                                    <  2:771201/140‑1 (MQ=255)
                                                                         tCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATc                                                                                                                              >  2:1151051/1‑78 (MQ=255)
                                                                         tCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTc                                                                                                       >  1:2878987/1‑101 (MQ=255)
                                                                         tCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTc                                                                                                       <  2:2878987/101‑1 (MQ=255)
                                                                         tCTTCACCGAATCCCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGAt                                                                >  1:908839/1‑140 (MQ=255)
                                                                           ttCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTc                                                              <  2:1305896/140‑1 (MQ=255)
                                                                              aCCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAg                                                           >  1:715690/1‑140 (MQ=255)
                                                                                  aaTCTCGAGGCAAAAAAGCATGTCATGCAGACAGCGCTTGAAAAAAGCTGCAAAAAGAAAGAAAACAACAGTTATATAGAAGCCAAAGGCAAGGGATCGGCAGGAACACACTTAAATGAAAGGAAAGAGATACAAGCCaa                                                       >  2:444574/1‑139 (MQ=255)
                                                                                    tctcGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCAttt                                                     >  1:2690785/1‑140 (MQ=255)
                                                                                         aGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGAGAAAGGCTCGGGATCGGCAGGAACAGACATACTTGAAATGAAGGCGATAAAAGACATTTAACGc                                                >  2:3012309/1‑140 (MQ=255)
                                                                                          ggCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCt                                               <  1:503793/140‑1 (MQ=255)
                                                                                          ggCACAAAAGGACGTCATGCTGTCAGCGCTTGAAAAAAGCGGCAAAAATAATGAAGAGATAAACTATATCGAAGCGAACGGATCGGGGTCGGCAGGAACAGATTTACTTGAATTAAAGGCAATCCAGTACAGTTACCgtg                                               >  2:2180622/1‑138 (MQ=255)
                                                                                           gaacaAAAGGATCTCATTCAGACAGCTCTTGAAAAAAGCGGCAAAAAGCATGAAGAGATCAGTTATCTCGAAGCGAATGGATCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTc                                              <  2:2691340/138‑1 (MQ=255)
                                                                                             acaAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAAAGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCg                                            >  2:1204001/1‑140 (MQ=255)
                                                                                             acaAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCAGAAGAGATAAGTTATCTCGAAGCGAATCGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTAAAGCCATTTACCgaaacg                                            >  2:821569/1‑135 (MQ=255)
                                                                                              caAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATAAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCgg                                           >  2:2684763/1‑140 (MQ=255)
                                                                                                     aTGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAg                                    >  2:2622699/1‑140 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   <  1:2665695/140‑1 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   <  1:2807473/140‑1 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   >  2:3036668/1‑140 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   <  1:887249/140‑1 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   <  1:210762/140‑1 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAgg                                   <  1:210789/140‑1 (MQ=255)
                                                                                                      tGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAACAGATCATTTATCTAGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAATCAATATACCGCTCCGGATCAAAgg                                   >  2:2336870/1‑140 (MQ=255)
                                                                                                       gTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGgc                                  <  1:3036668/140‑1 (MQ=255)
                                                                                                       gTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGgc                                  <  1:2336870/140‑1 (MQ=255)
                                                                                                         cATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGgcgc                                <  2:1011770/140‑1 (MQ=255)
                                                                                                         cATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGgcgc                                <  2:1942445/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:908839/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:2716432/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:1986432/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:1112924/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  1:94709/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  1:978847/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCGCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:1369702/140‑1 (MQ=255)
                                                                                                            gCAGACAGCGCGTGAAAAAAGCGGCAAAACGCCTGACGAGATCAGTTATCTCGAAGCGAAGGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCAt                             <  2:1729079/140‑1 (MQ=255)
                                                                                                             ctgtcAGCGCTTGAAAAAAGCGGCAAAAAGACTGAAGAGATCAGCTATCTCGAAGCGAACGGATCGGGGTCGGCAGTAACAGATTTACTTGAATTAAAGGCAATCCAGTCCATTTACCGTTCTGAATCAAAGGCTCCGtt                            <  1:2180622/136‑1 (MQ=255)
                                                                                                              tgtcAGCGCTTGAAAAAAGCGGCAAACAGACTGAAGAGATCAGCTATCTCGAAGCGAACGGATCGGGGTCGGCAGTAACAGATTTACTGGAATTAAAGGCAATCCAGTCCATTTACCGTTCTGAATCAAAGGCTCCGTTa                           <  2:1276843/137‑1 (MQ=255)
                                                                                                              aGACAGCGCTTGAAAAAAGCGGCAAACAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTa                           <  2:1806531/140‑1 (MQ=255)
                                                                                                              aGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTa                           <  1:3012309/140‑1 (MQ=255)
                                                                                                                    cgcTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATTGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGAGCAAAGGCGCCATTCGGGCTg                     >  2:1351397/1‑140 (MQ=255)
                                                                                                                    cgcTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTg                     >  1:1800755/1‑140 (MQ=255)
                                                                                                                    cgcTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTg                     >  2:2249870/1‑140 (MQ=255)
                                                                                                                    cgcTTGAAAAAAGCGGCAAAAAACATGAAGAGATCAGTTATCTCGAAGCGAATGGCTCCGGATCGGCAGTAACAGACTTACTTGAACTGAAGTCGATGCAACCCATTTACCGCTCAGGATCAAACGCGCCATTacgccag                     >  2:1787937/1‑134 (MQ=255)
                                                                                                                      cTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTggg                   <  1:740083/140‑1 (MQ=255)
                                                                                                                      cTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTggg                   <  1:2467132/140‑1 (MQ=255)
                                                                                                                      cTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTggg                   <  1:2836104/140‑1 (MQ=255)
                                                                                                                        tGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGAt                 <  2:2202810/140‑1 (MQ=255)
                                                                                                                             aaaTCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:2742838/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAACAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGTACTGAAGGCGATTCAAGCCATTTCCCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:468212/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAACAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  1:683456/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGCTCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  1:2034450/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAGGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:457543/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:1081198/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:722906/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:2402643/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:653164/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:591075/140‑1 (MQ=255)
                                                                                                                             aaaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAAATGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTg            <  2:3083835/140‑1 (MQ=255)
                                                                                                                              aaGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGa           >  1:1161725/1‑140 (MQ=255)
                                                                                                                                 cGGCAAAAATAATGAAGAAATGAGTTATATAGAAACGAATGGATCGGGATTGGCAGTAACAGAATTAAATGAACTGAAGGCGATTCAAACCATTTACAGCTCCGGATCAAAGTCGCCAATAGGGCAGGGATCACTGAAAc        >  2:2343302/1‑140 (MQ=255)
                                                                                                                                 cGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAAAGGCTCGGGATCGGCAGTAACCGACTTACATGAACTTAACGCGATTCAAGCCATTTACCGCACCGGATCAAAGGCGCCATTATGGCTGGGATCACTGAAAc        >  2:1538669/1‑140 (MQ=255)
                                                                                                                                 cGGCAAAAAGCCTGAAGAGATCAGTAATCTAGAACCGAAAAGCTCGGGATCGGCAGTAACAGAAATAATAGAACTGAAGGCGATTCAAGACATTTACCGCTCCGGATCAAAGGCGCCAATAGGCCAGGGATCACTGAAAc        >  2:745608/1‑140 (MQ=255)
                                                                                                                                 cGGCAAAAAGACTGAAGAGAACAGTTATCTCGAAGCGAAAGGCACCGGGTCGCCAGTAACAGAATTACATGAAATGAAGGCGATTCAAGCCATTTACAGCTCCGGATCAAAGGCGCCAATAGGGCTGGGAACAGTGcaaa        >  2:2620151/1‑139 (MQ=255)
                                                                                                                                  gtcAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACATACTTACTTGAACTGAAGGAGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAAcc       <  2:420722/138‑1 (MQ=255)
                                                                                                                                  ggCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAAcc       <  1:697719/140‑1 (MQ=255)
                                                                                                                                      aaaaGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAACCTAAc   <  1:886380/140‑1 (MQ=255)
                                                                                                                                       aaaGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAACCTAACa  >  2:1575777/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
CGAAGACGGAGATTCCATATATGCAGTTATTAAAGCCGCTGCTATTAACAATGACGGAAGAACAGCAGGCCCATCTTCACCGAATCTCGAGGCACAAAAGGATGTCATGCAGACAGCGCTTGAAAAAAGCGGCAAAAAGCCTGAAGAGATCAGTTATCTCGAAGCGAATGGCTCGGGATCGGCAGTAACAGACTTACTTGAACTGAAGGCGATTCAAGCCATTTACCGCTCCGGATCAAAGGCGCCATTAGGGCTGGGATCAGTGAAACCTAACA  >  NZ_CP034943/1789632‑1789906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: