Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,213 A→T 7.7% T288T (ACA→ACT parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,2130AT 7.7% 607.6 / 30.0 181T288T (ACA→ACTparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base A (75/92);  new base T (9/5);  total (84/97)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 188 total aligned reads displayed.

TCTCATTTGAGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCT  >  NZ_CP034943/1898074‑1898347
                                                                                                                                           |                                                                                                                                      
tctcATTTGAGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACa                                                                                                                                        >  1:2597719/1‑140 (MQ=255)
         aGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGaca                                                                                                                               >  2:254394/1‑140 (MQ=255)
         aGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGaca                                                                                                                               >  2:355936/1‑140 (MQ=255)
         aGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGaca                                                                                                                               >  2:380924/1‑140 (MQ=255)
           ggggAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACg                                                                                                                             <  2:593987/140‑1 (MQ=255)
              gggCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                          >  1:545647/3‑140 (MQ=255)
              gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGCCACGTGc                                                                                                                          >  2:1964706/1‑140 (MQ=255)
              gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                          >  1:2537024/1‑140 (MQ=255)
              gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                          >  1:2820542/1‑140 (MQ=255)
              gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATAGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                          >  1:1854143/1‑140 (MQ=255)
               aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                         <  2:2764877/140‑1 (MQ=255)
               aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                         <  2:2191916/140‑1 (MQ=255)
               aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                         <  2:1078062/140‑1 (MQ=255)
                gCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCtt                                                                                                                        >  1:648973/1‑140 (MQ=255)
                gCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGCTGCAAAAACAGCGATGACACGTGCtt                                                                                                                        >  2:3045220/1‑140 (MQ=255)
                 catcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCttt                                                                                                                       <  2:1008318/140‑1 (MQ=255)
                 catcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCttt                                                                                                                       <  2:1565895/140‑1 (MQ=255)
                 catcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAAAGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCttt                                                                                                                       <  2:1512308/140‑1 (MQ=255)
                   tcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTa                                                                                                                     <  2:2992958/140‑1 (MQ=255)
                      ttCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATg                                                                                                                  >  1:872033/1‑140 (MQ=255)
                      ttCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATg                                                                                                                  >  1:308042/1‑140 (MQ=255)
                      ttCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATg                                                                                                                  >  2:2325349/1‑140 (MQ=255)
                         aaTAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCTATGACACGTGCTTTTAATGAat                                                                                                               >  2:1139568/1‑140 (MQ=255)
                              aattGGACTGAGCGTTCCAGTTTAACGACGGCTATTCAGAAAACATGTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAAGGAATATgcg                                                                                                          <  2:1761266/137‑1 (MQ=255)
                              aaGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                          >  1:1113774/1‑140 (MQ=255)
                              aaGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                          >  2:2006254/1‑140 (MQ=255)
                              aaGTGGACTTTGAGTTACAGTTTAACTACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                          <  2:1180689/140‑1 (MQ=255)
                              aaGTGGACTGTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                          <  2:713560/140‑1 (MQ=255)
                                gTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACa                                                                                                                                        >  1:2288031/1‑108 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:1877528/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:2951705/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:2038006/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaca                                                                                                     >  1:2599963/1‑138 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  1:170697/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  1:308145/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  1:2717814/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:825300/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  1:416984/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:869657/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  2:944320/1‑140 (MQ=255)
                                   gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                     >  1:2365366/1‑140 (MQ=255)
                                     cTTTGCGTTCCAGTTTAACGACGGCTATTCTTAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTAATGAGTCAGGTGCAAAAACAGCGATGACACGTGATTTTAATGAATATGCGCGAAAAg                                                                                                   >  1:2249811/1‑140 (MQ=255)
                                        tGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTgg                                                                                                >  1:2886425/1‑140 (MQ=255)
                                             tCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTg                                                                                           >  2:1247076/1‑140 (MQ=255)
                                                 gTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTga                                                                                       <  2:61238/140‑1 (MQ=255)
                                                        gacgGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGaaaa                                                                                <  2:2164262/140‑1 (MQ=255)
                                                              tATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATaa                                                                          <  2:1113774/140‑1 (MQ=255)
                                                                     aaaaCATTTTATCGTTTGTTAACAACGTCAGAACGAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTg                                                                   >  1:1916635/1‑140 (MQ=255)
                                                                     aaaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTg                                                                   >  1:2810899/1‑140 (MQ=255)
                                                                      aaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTgg                                                                  <  2:2297315/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  2:3048961/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  1:1488192/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  1:273956/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  1:944320/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  1:1214948/140‑1 (MQ=255)
                                                                       aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                 <  1:825300/140‑1 (MQ=255)
                                                                         ccttttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAg                                                               <  1:1006819/138‑1 (MQ=255)
                                                                          aTTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAgg                                                              <  2:648973/140‑1 (MQ=255)
                                                                           ttttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGc                                                             <  2:116863/140‑1 (MQ=255)
                                                                            tttATCTTTTGTTAACAACGTCAGAACAAAAGACGGCGGTACTCATGAGTCAGGTGCAAAAACATCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  1:2152807/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  2:797602/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  1:1045356/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  2:2886425/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  1:668738/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  1:380924/140‑1 (MQ=255)
                                                                            tttATCGTTTGTTAACAACGTCAGAACAAAACACGGCGGGACTCATGAGTCAGGTGCAAAACCACCGATGACACGTGCTTTTAATGAAAATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                            <  1:1529301/140‑1 (MQ=255)
                                                                             ttATCTTTTGTTAACAACTTCATAACAAAATAAGTCTGGACTTATGAGTGAGTTTCTAAATCAGCGATGACACGTGCTTTTTATTTATATGCGCGAAAAGTGGGTTTGCTGAAAGAAAATGATTAAAACCTGGAAGGCAc                                                           <  2:1853636/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACTAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:1916635/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGCTGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:260156/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:977962/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  1:1439242/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:545647/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  1:2516760/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  1:323800/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:2810899/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  2:1636220/140‑1 (MQ=255)
                                                                             ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                           <  1:581866/140‑1 (MQ=255)
                                                                                  gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                      <  1:2006254/140‑1 (MQ=255)
                                                                                  gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                      <  2:308165/140‑1 (MQ=255)
                                                                                         aacaacGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATaaggga                                               <  1:155499/140‑1 (MQ=255)
                                                                                              cGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGc                                          >  1:2311060/1‑140 (MQ=255)
                                                                                                 cAGAACAAAAGACGGCGGGACTCCTGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGCAGATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATACAAACCTGGAAGGCACTGATATAAGGGAAGGGCttt                                       <  1:2167269/140‑1 (MQ=255)
                                                                                                   gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAACAACCTGGAAGGCACTGATATAAGGGAAGGTCTTTCa                                     <  2:2776185/140‑1 (MQ=255)
                                                                                                   gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATCTAAGGGAAGGGCTTTCa                                     >  2:923770/1‑140 (MQ=255)
                                                                                                   gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCa                                     <  2:2275551/140‑1 (MQ=255)
                                                                                                         aaGACGGCGGGCCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGAGATAAGGGAAGGGCTTTCAGCCatt                               >  1:727799/1‑140 (MQ=255)
                                                                                                         aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTTCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGACatt                               >  1:1559885/1‑140 (MQ=255)
                                                                                                              ggcggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTc                          >  1:2234998/1‑140 (MQ=255)
                                                                                                                    aCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCg                    <  1:75191/140‑1 (MQ=255)
                                                                                                                      tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                  <  2:319682/140‑1 (MQ=255)
                                                                                                                      tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                  <  2:2597719/140‑1 (MQ=255)
                                                                                                                      tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                  <  2:1573073/140‑1 (MQ=255)
                                                                                                                       cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                 <  1:1832469/140‑1 (MQ=255)
                                                                                                                       cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                 <  1:2038006/140‑1 (MQ=255)
                                                                                                                       cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                 <  1:1325836/140‑1 (MQ=255)
                                                                                                                       cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                 <  1:2951705/140‑1 (MQ=255)
                                                                                                                        aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                >  2:1405442/1‑140 (MQ=255)
                                                                                                                        aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                >  2:52132/1‑140 (MQ=255)
                                                                                                                               aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg         <  1:308751/140‑1 (MQ=255)
                                                                                                                                      aaaaCAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGcttct  >  2:40339/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TCTCATTTGAGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCT  >  NZ_CP034943/1898074‑1898347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: