Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,222 A→G 7.9% T291T (ACA→ACG parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,2220AG 7.9% 560.8 / 26.5 178T291T (ACA→ACGparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base A (70/93);  new base G (9/5);  total (79/99)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 188 total aligned reads displayed.

AGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGA  >  NZ_CP034943/1898083‑1898360
                                                                                                                                           |                                                                                                                                          
aGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGaca                                                                                                                                            >  2:355936/1‑140 (MQ=255)
aGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGaca                                                                                                                                            >  2:380924/1‑140 (MQ=255)
  ggggAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACg                                                                                                                                          <  2:1969109/140‑1 (MQ=255)
  ggggAGCATCAGTCAATAGCAGTGGACTTTGGGTTCCAGTTTAACGACGGCTAGTCTGAAAACATTTTATGGGTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCATGTGCAAAAACAGCGATGACACg                                                                                                                                          <  2:1881533/140‑1 (MQ=255)
     gggCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                                       >  1:545647/3‑140 (MQ=255)
     gAGCATCATTCAATAGAAGTTGACTTTGCGTTCAAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                                       >  1:3001644/1‑140 (MQ=255)
     gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                                       >  1:155413/1‑140 (MQ=255)
     gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGc                                                                                                                                       >  1:2820542/1‑140 (MQ=255)
     gAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGATATTCTGAAAACATTTTATCGTTTGTTAACAAAGTCAGAACAAAAGACGGAGCTACTCATGAGTCAGGTGCAAAAACAGCGATTACACTTGc                                                                                                                                       >  1:1853636/1‑140 (MQ=255)
      aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGTACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                                      <  2:2298836/140‑1 (MQ=255)
      aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                                      <  2:1078062/140‑1 (MQ=255)
      aGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCt                                                                                                                                      <  2:2764877/140‑1 (MQ=255)
       gCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGATGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCtt                                                                                                                                     >  2:2807102/1‑140 (MQ=255)
       gCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCtt                                                                                                                                     >  1:648973/1‑140 (MQ=255)
       gCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGCTGCAAAAACAGCGATGACACGTGCtt                                                                                                                                     >  2:3045220/1‑140 (MQ=255)
        catcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCttt                                                                                                                                    <  2:1008318/140‑1 (MQ=255)
          tcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTa                                                                                                                                  <  2:2992958/140‑1 (MQ=255)
          tcatTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTa                                                                                                                                  <  2:202832/140‑1 (MQ=255)
             ttCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATg                                                                                                                               >  1:308042/1‑140 (MQ=255)
             ttCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATg                                                                                                                               >  1:872033/1‑140 (MQ=255)
                aaTAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCTATGACACGTGCTTTTAATGAat                                                                                                                            >  2:1139568/1‑140 (MQ=255)
                     caGTGGACTGTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       <  2:1696119/139‑1 (MQ=255)
                     aaGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       <  2:1568477/140‑1 (MQ=255)
                     aaGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       >  1:1113774/1‑140 (MQ=255)
                     aaGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       <  2:58922/140‑1 (MQ=255)
                     aaGTGGACTTTGAGTTACAGTTTAACTACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       <  2:1180689/140‑1 (MQ=255)
                     aaGTGGACTGTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATgcg                                                                                                                       <  2:713560/140‑1 (MQ=255)
                       gTGGACTTTGCGTTCAAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAACACAGCGATGACACGTGCTTTTAATGAATATGcgcg                                                                                                                     >  2:542367/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:1325836/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:2038006/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:1832469/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGATGGTTATTCTGAAAATATTTTGTCGTTTGTCAACAATGTCAGAACAAAAGACGGCGGGACGCATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:2951705/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACCACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:2167269/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:825300/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  1:416984/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  1:308145/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:869657/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  2:944320/1‑140 (MQ=255)
                          gACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGaaa                                                                                                                  >  1:2717814/1‑140 (MQ=255)
                            cTTTGCGTTCCAGTTTAACGACGGCTATTCTTAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTAATGAGTCAGGTGCAAAAACAGCGATGACACGTGATTTTAATGAATATGCGCGAAAAg                                                                                                                >  1:2249811/1‑140 (MQ=255)
                            cTTTGCGTTCAATTTTAAAGACGGATATTCTTAAAAGATTTTATCGTTTTTTAACAAAGTCAGAACAAAATACGTCGGTAATCATTATTCAGGTGCAAAAACAGATATGACACGTTATTTTAATGAATATGCGCGAAAAg                                                                                                                <  2:2569269/140‑1 (MQ=255)
                               tGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTgg                                                                                                             >  1:2297315/1‑140 (MQ=255)
                               tGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTgg                                                                                                             >  1:2763040/1‑140 (MQ=255)
                               tGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTgg                                                                                                             >  1:1766275/1‑140 (MQ=255)
                               tGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTgg                                                                                                             >  1:2886425/1‑140 (MQ=255)
                               tGCGTTCCAGTTTAACGACGGCTATTCGGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTACTGAATATGCTCGAAAAGTgg                                                                                                             >  1:1490577/1‑140 (MQ=255)
                                    tCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTg                                                                                                        >  2:1247076/1‑140 (MQ=255)
                                        gTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTga                                                                                                    <  2:61238/140‑1 (MQ=255)
                                                     tATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCTATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATaa                                                                                       <  2:2879443/140‑1 (MQ=255)
                                                     tATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATaa                                                                                       <  2:1113774/140‑1 (MQ=255)
                                                            aaaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTg                                                                                >  1:2810899/1‑140 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:825300/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:944320/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:1947848/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:2728315/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:1214948/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  2:3048961/140‑1 (MQ=255)
                                                              aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                              <  1:2466321/140‑1 (MQ=255)
                                                                ccttttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAg                                                                            <  1:1006819/138‑1 (MQ=255)
                                                                 aTTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAgg                                                                           <  2:648973/140‑1 (MQ=255)
                                                                  ttttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGc                                                                          <  2:116863/140‑1 (MQ=255)
                                                                   tttATCTTTTGTTAACAACGTCAGAACAAAAGACGGCGGTACTCATGAGTCAGGTGCAAAAACATCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                         <  1:2152807/140‑1 (MQ=255)
                                                                   tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                         <  2:797602/140‑1 (MQ=255)
                                                                   tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                         <  1:1045356/140‑1 (MQ=255)
                                                                   tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                         <  1:380924/140‑1 (MQ=255)
                                                                   tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                         <  1:668738/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGACCAAAAGACGGCGCGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  2:2451295/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAATAGACGGCGGGACTCTTGAGTGAGGTGCAAAAACAGCGTTGACACGTGCTTTTTTTGAATAGGCGCGAAATGTGGCTTTGCTGAAAGAAAATGATAAACCGGTGGAAGGCAc                                                                        <  2:438937/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATTAAAACCTGGAAGGCAc                                                                        <  1:254394/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  1:25274/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  2:977962/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  1:1439242/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  1:323800/140‑1 (MQ=255)
                                                                    ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                        <  1:581866/140‑1 (MQ=255)
                                                                     tATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACt                                                                       <  1:2325349/140‑1 (MQ=255)
                                                                         gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                   <  2:308165/140‑1 (MQ=255)
                                                                         gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                   <  2:2599963/140‑1 (MQ=255)
                                                                            tGTTAACAACGTCAGAACCAAAGACGGGGGGACTCATGAGTCAGGTGCAAAAACAGCGATTAGACGTGCTTTTAATGCATATGCGCGAAAGGTGGCGTTGGTGACAGAAAAGGATAAAAACCTGGAAGGCCCTGatctca                                                                >  1:1648001/1‑136 (MQ=255)
                                                                                          gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATCTAAGGGAAGGGCTTTCa                                                  >  2:923770/1‑140 (MQ=255)
                                                                                          gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCa                                                  <  2:2275551/140‑1 (MQ=255)
                                                                                            aCAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAACAGGATAACAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGc                                                <  1:2204527/140‑1 (MQ=255)
                                                                                                aaGACGGCGGGCCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGAGATAAGGGAAGGGCTTTCAGCCatt                                            >  1:727799/1‑140 (MQ=255)
                                                                                                aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTTCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGACatt                                            >  1:1559885/1‑140 (MQ=255)
                                                                                                aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCatt                                            >  2:1957055/1‑140 (MQ=255)
                                                                                                     ggcggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTc                                       >  1:2234998/1‑140 (MQ=255)
                                                                                                        gggACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGt                                    <  2:2601880/140‑1 (MQ=255)
                                                                                                           aCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCg                                 <  1:75191/140‑1 (MQ=255)
                                                                                                             tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                               <  2:155730/140‑1 (MQ=255)
                                                                                                             tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                               <  2:1573073/140‑1 (MQ=255)
                                                                                                             tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                               <  2:319682/140‑1 (MQ=255)
                                                                                                              cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                              <  1:1325836/140‑1 (MQ=255)
                                                                                                              cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                              <  1:2951705/140‑1 (MQ=255)
                                                                                                              cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                              <  1:2038006/140‑1 (MQ=255)
                                                                                                              cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                              <  1:1877528/140‑1 (MQ=255)
                                                                                                               aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                             >  2:1871178/1‑140 (MQ=255)
                                                                                                                      aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                      <  1:308751/140‑1 (MQ=255)
                                                                                                                             aaaaCAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGcttct               >  2:40339/1‑140 (MQ=255)
                                                                                                                                     gATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTg       <  1:2613606/140‑1 (MQ=255)
                                                                                                                                      ctGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGa      <  2:2215517/139‑1 (MQ=255)
                                                                                                                                          aacGTGCTTTTATTTAATATGCGCGTAAAGTTGTTTTGCTGTTAGAATAGGATAATAACCTGGATGGCTGTGATTTAAGGGAAGGGCTTTCAGCAATTATTTCTTTTCGGATTCCTGAGGAGCTTATTCAATTTGAAGGa  <  2:1510633/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AGGGGGAGCATCATTCAATAGAAGTGGACTTTGCGTTCCAGTTTAACGACGGCTATTCTGAAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGA  >  NZ_CP034943/1898083‑1898360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: