Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 266,857 T→C 7.6% F54F (TTT→TTC kdgD → 5‑dehydro‑4‑deoxyglucarate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943266,8570TC 7.6% 583.9 / 26.1 183F54F (TTT→TTCkdgD5‑dehydro‑4‑deoxyglucarate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (88/81);  new base C (7/7);  total (95/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 188 total aligned reads displayed.

GCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGCGAAAAA  >  NZ_CP034943/266720‑266994
                                                                                                                                         |                                                                                                                                         
gCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCa                                                                                                                                         >  2:805667/1‑140 (MQ=255)
     aaTCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAAtctc                                                                                                                                    >  2:1231741/1‑140 (MQ=255)
            ggTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCa                                                                                                                             >  2:3018202/1‑140 (MQ=255)
                ttCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaa                                                                                                                         >  2:192315/1‑140 (MQ=255)
                ttCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaa                                                                                                                         >  2:485771/1‑140 (MQ=255)
                ttCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaa                                                                                                                         >  2:1993114/1‑140 (MQ=255)
                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagacgg                                                                                                                        >  2:2667837/1‑137 (MQ=255)
                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCagaagg                                                                                                                        >  2:159442/1‑138 (MQ=255)
                 tCCGGTTGCTCCTTTTAACACACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAACATTGAGTTTTTGCTGAATGAGGGCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATATCTCAGCCagaagg                                                                                                                        >  2:1473891/1‑138 (MQ=255)
                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCagaaag                                                                                                                        >  2:1410098/1‑140 (MQ=255)
                 tCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCaga                                                                                                                           >  1:2868433/1‑137 (MQ=255)
                   cgtttGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGGTTGGAAGCGATTTTTATCGCTTGTGGGTATGGTGAATTTCAATCTCTCAGCCagaaagaa                                                                                                                      <  2:1814133/137‑1 (MQ=255)
                       tGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATa                                                                                                                    >  1:1549002/1‑138 (MQ=255)
                           ccTTTTAACTCACAAGGAACACTGGAGGAAGCAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTTGAAGCGATTTTTATCGCTTGTGTGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCa                                                                                                              <  1:1619081/140‑1 (MQ=255)
                              tttAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAAt                                                                                                           >  1:2560555/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGTAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  2:1565189/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTTGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCATAAAGAATACGAGCAAATGGTGGacg                                                                                                   >  2:1183687/1‑138 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  2:240483/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  1:2736981/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  2:2646031/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  2:122901/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  1:891750/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  1:1493489/1‑140 (MQ=255)
                                      acaAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAg                                                                                                   >  1:1336892/1‑140 (MQ=255)
                                       caAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGt                                                                                                  >  2:3088433/1‑140 (MQ=255)
                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                                            >  1:2828769/1‑129 (MQ=255)
                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                                            <  2:2828769/129‑1 (MQ=255)
                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCaaa                                                                                                            <  2:2333430/129‑1 (MQ=255)
                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                                                 <  2:477498/140‑1 (MQ=255)
                                        aaGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTc                                                                                                 <  2:2140385/140‑1 (MQ=255)
                                                  ggaggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCgg                                                                                       >  1:368989/1‑140 (MQ=255)
                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCTGCCAGAAAGAATACGAGCAAATGGTGGAAGTAGCACTCTCGGCAg                                                                                    >  2:2340399/1‑140 (MQ=255)
                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                                                    >  2:2176543/1‑140 (MQ=255)
                                                     ggaAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGAGTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAg                                                                                    >  2:2345765/1‑140 (MQ=255)
                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                                                   >  2:2490601/1‑140 (MQ=255)
                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                                                   >  2:2011957/1‑140 (MQ=255)
                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                                                   >  1:329375/1‑140 (MQ=255)
                                                      gaagaaGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                                                   >  1:1967811/1‑140 (MQ=255)
                                                             ctctTTTTCAAAATCTCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCggagga                                                                            >  1:707622/1‑140 (MQ=255)
                                                             ctctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCggagga                                                                            <  2:500179/140‑1 (MQ=255)
                                                              tctTTTTCAAAATATCGTGTTTTTTGTTGAAGTATGCTTTGAAGCTATTTTTATCGCTTGTTGTTATGGTGAATTTCAATCTCTCAGGCAGAAAGAATACTAGCAAATGGTGGTAGTAGCAATCTCGGCAGCCGTTGGaa                                                                           <  2:1232784/140‑1 (MQ=255)
                                                              tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                                           <  2:855500/140‑1 (MQ=255)
                                                              tctTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGaa                                                                           <  1:3018202/140‑1 (MQ=255)
                                                                tttttCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAg                                                                         >  2:1706522/1‑140 (MQ=255)
                                                                    tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                                                     <  2:826888/140‑1 (MQ=255)
                                                                    tCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTc                                                                     <  2:2769879/140‑1 (MQ=255)
                                                                     cAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGCGGAAAGGTTcc                                                                    >  2:2731497/1‑140 (MQ=255)
                                                                     cAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTcc                                                                    >  2:3056195/1‑140 (MQ=255)
                                                                      aaaaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTTAATTTCAATCTCTCAGCCATAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCg                                                                   <  2:2434714/140‑1 (MQ=255)
                                                                        aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                                                 <  1:1240321/140‑1 (MQ=255)
                                                                        aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                                                 <  1:2702875/140‑1 (MQ=255)
                                                                        aaTATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGt                                                                 <  1:2702991/140‑1 (MQ=255)
                                                                            tCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTac                                                             >  2:2674933/1‑140 (MQ=255)
                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                                            <  2:707622/140‑1 (MQ=255)
                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                                            <  1:3088433/140‑1 (MQ=255)
                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                                            <  1:922382/140‑1 (MQ=255)
                                                                             cGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTaca                                                            <  1:739902/140‑1 (MQ=255)
                                                                                          gaagaaGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcgg                                               >  2:130979/1‑140 (MQ=255)
                                                                                           aagaagGCTTGGAATCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTTGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:2069010/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:240483/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:16692/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:2418771/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:1610417/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:1937247/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:47438/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:512618/140‑1 (MQ=255)
                                                                                           aagaagGCTTGGAAGCGATTTTTATCGCTCGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGggcggc                                              <  1:1187738/140‑1 (MQ=255)
                                                                                              aagGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAAc                                           <  2:295284/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                                         <  1:1375339/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                                         <  1:2997327/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCt                                         <  1:239446/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                                         <  1:1230601/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                                         <  1:489429/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGATATTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                                         <  1:2471353/140‑1 (MQ=255)
                                                                                                ggCTTGGAAGCGAGAGTTATCGCTTGCGGGTCTGGTGAATTCCAATCTCTCAGCCAGAAGGAATATGAGCAAATGGTGGAAGTCGCAGTCTCGGCAGCCGGAGGAAAGGTGCCGGTCTACACGGGGGTGGGCGGCAACCt                                         <  1:2667837/140‑1 (MQ=255)
                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa                                    <  1:2135580/140‑1 (MQ=255)
                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa                                    <  1:1410098/140‑1 (MQ=255)
                                                                                                     ggAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTa                                    <  1:417215/140‑1 (MQ=255)
                                                                                                       aaGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACa                                  <  1:251172/140‑1 (MQ=255)
                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGc                                                                                   >  2:1618773/1‑86 (MQ=255)
                                                                                                            aTTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACt                             >  1:1033954/1‑140 (MQ=255)
                                                                                                                 tATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAt                        <  1:192315/140‑1 (MQ=255)
                                                                                                                 tATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAt                        <  1:1993114/140‑1 (MQ=255)
                                                                                                                 tATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAAt                        <  1:2665311/140‑1 (MQ=255)
                                                                                                                               cTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCg          <  1:87780/140‑1 (MQ=255)
                                                                                                                               cTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCg          <  1:1208887/140‑1 (MQ=255)
                                                                                                                               cTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTGCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCg          <  1:2011957/140‑1 (MQ=255)
                                                                                                                                tGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCgg         >  2:2133164/1‑140 (MQ=255)
                                                                                                                                tGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCgg         >  2:1124767/1‑140 (MQ=255)
                                                                                                                                tGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCgg         >  1:99640/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCTCAATCTCGGCAGCCGGAGGAAAGGTTCCTGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:3041680/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  2:1662770/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:2245939/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:2081917/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  2:643160/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:1721754/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:1429313/1‑140 (MQ=255)
                                                                                                                                 ggTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGc        >  1:1327713/1‑140 (MQ=255)
                                                                                                                                        ttCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGtt                                                                      <  1:933163/71‑1 (MQ=255)
                                                                                                                                        ttCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGtt                                                                      >  2:933163/1‑71 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GCTGGAATCTTAGGTTTTCCGGTTGCACCTTTTAACTCACAAGGAAAACTGGAGGAAGAAGCTCTTTTTCAAAATATCGAGTTTTTGGTGGAAGAAGGCTTGGAAGCGATTTTTATCGCTTGTGGGTCTGGTGAATTTCAATCTCTCAGCCAGAAAGAATACGAGCAAATGGTGGAAGTCGCAATCTCGGCAGCCGGAGGAAAGGTTCCGGTTTACACGGGGGTGGGCGGCAACCTCAGTACAGCACTTGAATGGGCGCAGCTGTCGGCGAAAAA  >  NZ_CP034943/266720‑266994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: