Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,282 G→T 6.2% L311L (CTG→CTT parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,2820GT 6.2% 430.0 / 15.4 145L311L (CTG→CTTparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base G (39/97);  new base T (4/5);  total (43/102)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 149 total aligned reads displayed.

AAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCA  >  NZ_CP034943/1898143‑1898420
                                                                                                                                           |                                                                                                                                          
aaaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATCAAAACCTg                                                                                                                                            >  1:2894040/1‑140 (MQ=255)
aaaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTg                                                                                                                                            >  1:2810899/1‑140 (MQ=255)
 aaaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTgg                                                                                                                                           <  2:2297315/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:273956/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:944320/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:869657/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:825300/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:1488192/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:1947848/140‑1 (MQ=255)
  aaCATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACAAGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGa                                                                                                                                          <  1:2864452/140‑1 (MQ=255)
  aaCATTTTATAGTTTGTTAACAACGTCATATCTAAAGTCGGCGGGACTCATGAGTCAGGTGGAAAAACAGGGATGACTCGTGCTTTTAATGAATATTCGCGAAAAGTTTGTTTGCTGAAAGAAAAGGATACTAACCTGGa                                                                                                                                          <  2:3048384/140‑1 (MQ=255)
    ccttttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAg                                                                                                                                        <  1:1006819/138‑1 (MQ=255)
     aTTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAgg                                                                                                                                       <  2:648973/140‑1 (MQ=255)
       tttATCTTTTGTTAACAACGTCAGAACAAAAGACGGCGGTACTCATGAGTCAGGTGCAAAAACATCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                                                                                     <  1:2152807/140‑1 (MQ=255)
       tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                                                                                     <  2:2886425/140‑1 (MQ=255)
       tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                                                                                     <  1:355936/140‑1 (MQ=255)
       tttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCa                                                                                                                                     <  1:668738/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAATAGACGGCGGGACTCTTGAGTGAGGTGCAAAAACAGCGTTGACACGTGCTTTTTTTGAATAGGCGCGAAATGTGGCTTTGCTGAAAGAAAATGATAAACCGGTGGAAGGCAc                                                                                                                                    <  2:438937/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAGAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:73792/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:323800/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:581866/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  2:1636220/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  2:2810899/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  2:977962/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:2516760/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:1215007/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:1439242/140‑1 (MQ=255)
        ttATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCAc                                                                                                                                    <  1:25274/140‑1 (MQ=255)
         tATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACt                                                                                                                                   <  1:2325349/140‑1 (MQ=255)
             gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                               <  2:308165/140‑1 (MQ=255)
             gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                               <  2:308145/140‑1 (MQ=255)
             gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                               <  2:2599963/140‑1 (MQ=255)
             gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                               <  1:2006254/140‑1 (MQ=255)
                tGTTAACAACGTCAGAACCAAAGACGGGGGGACTCATGAGTCAGGTGCAAAAACAGCGATTAGACGTGCTTTTAATGCATATGCGCGAAAGGTGGCGTTGGTGACAGAAAAGGATAAAAACCTGGAAGGCCCTGatctca                                                                                                                            >  1:1648001/1‑136 (MQ=255)
                    aacaacGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATaaggga                                                                                                                        <  1:155499/140‑1 (MQ=255)
                         cGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGc                                                                                                                   >  1:2311060/1‑140 (MQ=255)
                            cAGAACAAAAGACGGCGGGACTCCTGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGCAGATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATACAAACCTGGAAGGCACTGATATAAGGGAAGGGCttt                                                                                                                <  1:2167269/140‑1 (MQ=255)
                              gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAACAACCTGGAAGGCACTGATATAAGGGAAGGTCTTTCa                                                                                                              <  2:2776185/140‑1 (MQ=255)
                                    aaGACGGCGGGCCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGAGATAAGGGAAGGGCTTTCAGCCatt                                                                                                        >  1:727799/1‑140 (MQ=255)
                                    aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCatt                                                                                                        >  2:719848/1‑140 (MQ=255)
                                    aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCatt                                                                                                        >  2:1957055/1‑140 (MQ=255)
                                         ggcggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTc                                                                                                   >  1:2234998/1‑140 (MQ=255)
                                           cggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTg                                                                                                 >  1:997736/1‑140 (MQ=255)
                                            gggACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGt                                                                                                <  2:2601880/140‑1 (MQ=255)
                                                 tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                           <  2:1573073/140‑1 (MQ=255)
                                                 tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                           <  2:2597719/140‑1 (MQ=255)
                                                  cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                          <  1:1325836/140‑1 (MQ=255)
                                                  cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                          <  1:1877528/140‑1 (MQ=255)
                                                  cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                          <  1:2951705/140‑1 (MQ=255)
                                                   aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                         >  2:52132/1‑140 (MQ=255)
                                                   aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                         >  2:1871178/1‑140 (MQ=255)
                                                   aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                         >  2:1405442/1‑140 (MQ=255)
                                                          aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAACGGATAAAAACCTGGAAGGAACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                  >  2:955686/1‑140 (MQ=255)
                                                          aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                  >  2:798323/1‑140 (MQ=255)
                                                                 aaaaCAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGcttct                                                                           >  2:40339/1‑140 (MQ=255)
                                                                         gATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTg                                                                   <  1:2613606/140‑1 (MQ=255)
                                                                          ctGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGa                                                                  <  2:2215517/139‑1 (MQ=255)
                                                                              cacGTGCTTTTAATGAATATGCTCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                              <  2:1285917/140‑1 (MQ=255)
                                                                              cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                              <  1:3045220/140‑1 (MQ=255)
                                                                              cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                              <  2:434074/140‑1 (MQ=255)
                                                                              aacGTGCTTTTATTTAATATGCGCGTAAAGTTGTTTTGCTGTTAGAATAGGATAATAACCTGGATGGCTGTGATTTAAGGGAAGGGCTTTCAGCAATTATTTCTTTTCGGATTCCTGAGGAGCTTATTCAATTTGAAGGa                                                              <  2:1510633/139‑1 (MQ=255)
                                                                                  tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTTAAGGacaaa                                                          >  1:1546195/1‑140 (MQ=255)
                                                                                  tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                          >  1:2234547/1‑140 (MQ=255)
                                                                                  tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                          >  1:160142/1‑140 (MQ=255)
                                                                                  tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                          >  2:1309337/1‑140 (MQ=255)
                                                                                  tGCTTTTAATGAATATGCGCGAAAAGTGGCTTGGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                          <  2:2440801/140‑1 (MQ=255)
                                                                                          aTGAATATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                  <  1:3005284/140‑1 (MQ=255)
                                                                                          aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                  <  2:3001644/140‑1 (MQ=255)
                                                                                          aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                  <  2:2820542/140‑1 (MQ=255)
                                                                                          aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                  <  1:1141572/140‑1 (MQ=255)
                                                                                          aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                  <  2:155413/140‑1 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGTATAAATACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             <  2:1729870/140‑1 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCt                                             >  2:2693808/1‑140 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             >  2:2523820/1‑140 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             >  1:1028469/1‑140 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             >  2:114287/1‑140 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             >  2:113953/1‑140 (MQ=255)
                                                                                               tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             >  2:1231209/1‑140 (MQ=255)
                                                                                               taggcgcGAAAAGTGGCTTTGCTGACAGAACAGGATAAAAACCTGGAAGGCACTGAGATAAGCGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                             <  2:1742138/137‑1 (MQ=255)
                                                                                                  gcgcGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTgg                                          <  2:880417/140‑1 (MQ=255)
                                                                                                   cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                         >  2:2692236/1‑140 (MQ=255)
                                                                                                   cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                         >  2:2482000/1‑140 (MQ=255)
                                                                                                   cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTGTCAGCCATTATTTCTGTTCGGATTCATGAGGAGCTTCTTCAATTTGAAGGACAAACCAAAGGAAAGCTTGGt                                         >  2:558074/1‑140 (MQ=255)
                                                                                                    gcgAAAAGTGGCTTTGCTGAAAGAAAAGGCTAAACACCTGGAAGGCACTGATATAAGGCAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTa                                        <  2:537672/140‑1 (MQ=255)
                                                                                                    gcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAACACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTa                                        <  1:2626039/140‑1 (MQ=255)
                                                                                                       aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                     >  2:2291491/1‑140 (MQ=255)
                                                                                                       aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                     >  2:1912309/1‑140 (MQ=255)
                                                                                                              gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                              <  1:1014778/140‑1 (MQ=255)
                                                                                                              gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                              >  2:464770/1‑140 (MQ=255)
                                                                                                              gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                              <  1:1862183/140‑1 (MQ=255)
                                                                                                                  tGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCgaga                          >  1:1146662/1‑140 (MQ=255)
                                                                                                                    cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                        <  2:37755/140‑1 (MQ=255)
                                                                                                                    cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                        <  2:1462890/140‑1 (MQ=255)
                                                                                                                    cTGAAAGAAAAGGATAAAAACATGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGTGGAGATTCTTCAATTTGAAGGACAAACATCAGGAAAGCTTGGTACTAGTGAAGCGAGATc                        <  2:730315/140‑1 (MQ=255)
                                                                                                                      gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                      <  2:479915/140‑1 (MQ=255)
                                                                                                                      gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                      <  2:2357278/140‑1 (MQ=255)
                                                                                                                          gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                  <  2:2763040/140‑1 (MQ=255)
                                                                                                                          gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                  <  2:1766275/140‑1 (MQ=255)
                                                                                                                                   aaaaaCCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGgt         <  1:2743060/140‑1 (MQ=255)
                                                                                                                                          tGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCa  >  2:1700758/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
AAAACATTTTATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCA  >  NZ_CP034943/1898143‑1898420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: