Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,291 T→C 8.2% T314T (ACT→ACC parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,2910TC 8.2% 341.5 / 16.2 110T314T (ACT→ACCparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base T (46/55);  new base C (4/5);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

Only 100 of 111 total aligned reads displayed.

TATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTT  >  NZ_CP034943/1898152‑1898429
                                                                                                                                           |                                                                                                                                          
tATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACt                                                                                                                                            <  1:2325349/140‑1 (MQ=255)
    gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                                        <  2:308165/140‑1 (MQ=255)
    gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                                        <  1:2006254/140‑1 (MQ=255)
    gTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGata                                                                                                                                        <  2:2599963/140‑1 (MQ=255)
       tGTTAACAACGTCAGAACCAAAGACGGGGGGACTCATGAGTCAGGTGCAAAAACAGCGATTAGACGTGCTTTTAATGCATATGCGCGAAAGGTGGCGTTGGTGACAGAAAAGGATAAAAACCTGGAAGGCCCTGatctca                                                                                                                                     >  1:1648001/1‑136 (MQ=255)
           aacaacGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATaaggga                                                                                                                                 <  1:155499/140‑1 (MQ=255)
                cGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGc                                                                                                                            >  1:2311060/1‑140 (MQ=255)
                   cAGAACAAAAGACGGCGGGACTCCTGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGCAGATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATACAAACCTGGAAGGCACTGATATAAGGGAAGGGCttt                                                                                                                         <  1:2167269/140‑1 (MQ=255)
                     gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAACAACCTGGAAGGCACTGATATAAGGGAAGGTCTTTCa                                                                                                                       <  2:2776185/140‑1 (MQ=255)
                     gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATCTAAGGGAAGGGCTTTCa                                                                                                                       >  2:923770/1‑140 (MQ=255)
                     gAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCa                                                                                                                       <  2:2275551/140‑1 (MQ=255)
                          aaaGCCGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCat                                                                                                                  <  1:2248017/140‑1 (MQ=255)
                           aaGACGGCGGGCCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGAGATAAGGGAAGGGCTTTCAGCCatt                                                                                                                 >  1:727799/1‑140 (MQ=255)
                           aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTTCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGACatt                                                                                                                 >  1:1559885/1‑140 (MQ=255)
                           aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCatt                                                                                                                 >  2:1957055/1‑140 (MQ=255)
                           aaGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCatt                                                                                                                 >  2:719848/1‑140 (MQ=255)
                                ggcggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTc                                                                                                            >  1:2234998/1‑140 (MQ=255)
                                  cggGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTg                                                                                                          >  1:997736/1‑140 (MQ=255)
                                   gggACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGt                                                                                                         <  2:2601880/140‑1 (MQ=255)
                                      aCTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCg                                                                                                      <  1:75191/140‑1 (MQ=255)
                                        tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                    <  2:2597719/140‑1 (MQ=255)
                                        tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                    <  2:155730/140‑1 (MQ=255)
                                        tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                    <  2:1573073/140‑1 (MQ=255)
                                        tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                    <  2:319682/140‑1 (MQ=255)
                                         cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                   <  1:2038006/140‑1 (MQ=255)
                                         cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                   <  1:1325836/140‑1 (MQ=255)
                                         cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                   <  1:1832469/140‑1 (MQ=255)
                                         cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                   <  1:1877528/140‑1 (MQ=255)
                                         cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                   <  1:2951705/140‑1 (MQ=255)
                                          aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                  >  2:1405442/1‑140 (MQ=255)
                                          aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                  >  2:704458/1‑140 (MQ=255)
                                          aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                  >  2:52132/1‑140 (MQ=255)
                                          aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                  >  2:1871178/1‑140 (MQ=255)
                                                 aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                           <  1:308751/140‑1 (MQ=255)
                                                 aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                           >  2:1014778/1‑140 (MQ=255)
                                                 aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                           >  2:798323/1‑140 (MQ=255)
                                                        aaaaCAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGcttct                                                                                    >  2:40339/1‑140 (MQ=255)
                                                                gATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTg                                                                            <  1:2613606/140‑1 (MQ=255)
                                                                 ctGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGa                                                                           <  2:2215517/139‑1 (MQ=255)
                                                                     cacGTGCTTTTAATGAATATGCTCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                       <  2:1285917/140‑1 (MQ=255)
                                                                     cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                       <  1:2995391/140‑1 (MQ=255)
                                                                     cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                       <  1:3045220/140‑1 (MQ=255)
                                                                     cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                       <  2:434074/140‑1 (MQ=255)
                                                                     cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                       <  1:2807102/140‑1 (MQ=255)
                                                                     aacGTGCTTTTATTTAATATGCGCGTAAAGTTGTTTTGCTGTTAGAATAGGATAATAACCTGGATGGCTGTGATTTAAGGGAAGGGCTTTCAGCAATTATTTCTTTTCGGATTCCTGAGGAGCTTATTCAATTTGAAGGa                                                                       <  2:1510633/139‑1 (MQ=255)
                                                                         tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTTAAGGacaaa                                                                   >  1:1546195/1‑140 (MQ=255)
                                                                         tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                                   >  1:2234547/1‑140 (MQ=255)
                                                                         tGCTTTTAATGAATATGCGCGAAAAGTGGCTTGGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                                   <  2:2440801/140‑1 (MQ=255)
                                                                                aaTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAgg                                                            >  1:139639/1‑140 (MQ=255)
                                                                                 aTGAATATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  1:3005284/140‑1 (MQ=255)
                                                                                 aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  2:2820542/140‑1 (MQ=255)
                                                                                 aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  2:3001644/140‑1 (MQ=255)
                                                                                 aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  2:155413/140‑1 (MQ=255)
                                                                                 aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  1:1141572/140‑1 (MQ=255)
                                                                                 aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCGTCTTCAATTTGAAGGACAAACAAAAGGa                                                           <  2:2537024/140‑1 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCt                                                      >  2:2693808/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  2:450209/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  1:1028469/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      <  2:1290213/140‑1 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  2:113953/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  2:114287/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  2:2523820/1‑140 (MQ=255)
                                                                                      tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      >  2:1231209/1‑140 (MQ=255)
                                                                                      taggcgcGAAAAGTGGCTTTGCTGACAGAACAGGATAAAAACCTGGAAGGCACTGAGATAAGCGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                      <  2:1742138/137‑1 (MQ=255)
                                                                                         gcgcGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTgg                                                   <  2:880417/140‑1 (MQ=255)
                                                                                          cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                                  >  2:2692236/1‑140 (MQ=255)
                                                                                          cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                                  >  2:2482000/1‑140 (MQ=255)
                                                                                          cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTGTCAGCCATTATTTCTGTTCGGATTCATGAGGAGCTTCTTCAATTTGAAGGACAAACCAAAGGAAAGCTTGGt                                                  >  2:558074/1‑140 (MQ=255)
                                                                                           gcgAAAAGTGGCTTTGCTGAAAGAAAAGGCTAAACACCTGGAAGGCACTGATATAAGGCAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTa                                                 <  2:537672/140‑1 (MQ=255)
                                                                                           gcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAACACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTa                                                 <  1:2626039/140‑1 (MQ=255)
                                                                                              aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                              >  2:2291491/1‑140 (MQ=255)
                                                                                              aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                              >  2:1912309/1‑140 (MQ=255)
                                                                                                     gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                       <  1:1862183/140‑1 (MQ=255)
                                                                                                     gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                       >  2:464770/1‑140 (MQ=255)
                                                                                                     gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                       <  1:2864960/140‑1 (MQ=255)
                                                                                                     gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                       <  1:1014778/140‑1 (MQ=255)
                                                                                                        ttGCTGATAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGATGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAAGTTGAAGGACAACCAAAAGGAAAGCTTGGTACTAGTGAAGCgag                                    <  2:1463282/140‑1 (MQ=255)
                                                                                                         tGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCgaga                                   >  1:1146662/1‑140 (MQ=255)
                                                                                                           cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                 <  2:37755/140‑1 (MQ=255)
                                                                                                           cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                 <  2:1462890/140‑1 (MQ=255)
                                                                                                           cTGAAAGAAAAGGATAAAAACATGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGTGGAGATTCTTCAATTTGAAGGACAAACATCAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                 <  2:730315/140‑1 (MQ=255)
                                                                                                             gaaagaaaAGGCTAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                               <  2:2909248/140‑1 (MQ=255)
                                                                                                             gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                               <  2:479915/140‑1 (MQ=255)
                                                                                                             gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                               <  2:2357278/140‑1 (MQ=255)
                                                                                                             gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCGTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                               <  2:2769316/140‑1 (MQ=255)
                                                                                                              aaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcagc                              <  2:2818880/140‑1 (MQ=255)
                                                                                                                 gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                           <  2:1766275/140‑1 (MQ=255)
                                                                                                                 gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                           <  2:411601/140‑1 (MQ=255)
                                                                                                                 gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                           <  2:2763040/140‑1 (MQ=255)
                                                                                                                     aGGATAAAAACCTGGAAGGCCCTGATATAAGGGAAGGGCTTTCAGCCATTCTGTCTGTTCGGAGTCCTGAGGAGCTTCTTCAATGTGAGGGACAAACAAATGGCACGCTTGGTCCGAGTGACGCGAGATCAGCAGtgtat                       >  2:1642648/1‑136 (MQ=255)
                                                                                                                        aTAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCg                    >  2:1740310/1‑140 (MQ=255)
                                                                                                                                 tGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCa           >  2:1700758/1‑140 (MQ=255)
                                                                                                                                       gCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcag     <  1:1964706/140‑1 (MQ=255)
                                                                                                                                        cACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcagc    >  1:449744/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt  >  1:2291677/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt  >  1:2291787/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt  >  2:2991531/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt  >  1:2900637/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCTTTCAGCCATTATTTATGTTCGGATTCCTGAGGAGCTTCTTCAATATGAAGGACAAAAAAAAGGAAAGCTTGGTACTAGTCAAGCGAGAACACCAGATGATGCGGTTGTTTCAGTGCAgctt  >  1:1737881/1‑140 (MQ=255)
                                                                                                                                          cTGATATAAGGGAAGGGCGTTCAGCCATTATTTCTGTTCGGATTCCTGATGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt  >  1:1367737/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TATCGTTTGTTAACAACGTCAGAACAAAAGACGGCGGGACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTT  >  NZ_CP034943/1898152‑1898429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: