Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,327 T→C 5.7% V326V (GTT→GTC parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,3270TC 5.7% 522.6 / 13.1 156V326V (GTT→GTCparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base T (88/59);  new base C (4/5);  total (92/64)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

ACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACG  >  NZ_CP034943/1898190‑1898465
                                                                                                                                         |                                                                                                                                          
  tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                                                        <  2:1573073/140‑1 (MQ=255)
   cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:2951705/140‑1 (MQ=255)
   cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:2038006/140‑1 (MQ=255)
   cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:1325836/140‑1 (MQ=255)
   cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:1877528/140‑1 (MQ=255)
    aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                                                      >  2:704458/1‑140 (MQ=255)
           aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAACGGATAAAAACCTGGAAGGAACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               >  2:955686/1‑140 (MQ=255)
           aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               <  1:308751/140‑1 (MQ=255)
           aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               >  2:1014778/1‑140 (MQ=255)
                          gATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTg                                                                                                                <  1:2613606/140‑1 (MQ=255)
                               cacGTGCTTTTAATGAATATGCTCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  2:1285917/140‑1 (MQ=255)
                               cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  1:2995391/140‑1 (MQ=255)
                               cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  2:434074/140‑1 (MQ=255)
                               cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  1:2807102/140‑1 (MQ=255)
                                   tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTTAAGGacaaa                                                                                                       >  1:1546195/1‑140 (MQ=255)
                                   tGCTTTTAATGAATATGCGCGAAAAGTGGCTTGGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                                                                       <  2:2440801/140‑1 (MQ=255)
                                          aaTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAgg                                                                                                >  1:139639/1‑140 (MQ=255)
                                           aTGAATATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  1:3005284/140‑1 (MQ=255)
                                           aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  2:155413/140‑1 (MQ=255)
                                           aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  1:1141572/140‑1 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCt                                                                                          >  2:2693808/1‑140 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:113953/1‑140 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  2:1290213/140‑1 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:450209/1‑140 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:2523820/1‑140 (MQ=255)
                                                tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  1:1028469/1‑140 (MQ=255)
                                                    cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                                                                      >  2:2482000/1‑140 (MQ=255)
                                                    cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                                                                      >  2:2692236/1‑140 (MQ=255)
                                                    cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTGTCAGCCATTATTTCTGTTCGGATTCATGAGGAGCTTCTTCAATTTGAAGGACAAACCAAAGGAAAGCTTGGt                                                                                      >  2:558074/1‑140 (MQ=255)
                                                     gcgAAAAGTGGCTTTGCTGAAAGAAAAGGCTAAACACCTGGAAGGCACTGATATAAGGCAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTa                                                                                     <  2:537672/140‑1 (MQ=255)
                                                     gcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAACACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTa                                                                                     <  1:2626039/140‑1 (MQ=255)
                                                        aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                                                                  >  2:1912309/1‑140 (MQ=255)
                                                               gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATCTAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           >  2:484734/1‑140 (MQ=255)
                                                               gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:2864960/140‑1 (MQ=255)
                                                               gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:1862183/140‑1 (MQ=255)
                                                               gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:1014778/140‑1 (MQ=255)
                                                                  ttGCTGATAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGATGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAAGTTGAAGGACAACCAAAAGGAAAGCTTGGTACTAGTGAAGCgag                                                                        <  2:1463282/140‑1 (MQ=255)
                                                                     cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                                                     <  2:1462890/140‑1 (MQ=255)
                                                                     cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                                                     <  2:37755/140‑1 (MQ=255)
                                                                     cTGAAAGAAAAGGATAAAAACATGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGTGGAGATTCTTCAATTTGAAGGACAAACATCAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                                                     <  2:730315/140‑1 (MQ=255)
                                                                       gaaagaaaAGGCTAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:2909248/140‑1 (MQ=255)
                                                                       gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:479915/140‑1 (MQ=255)
                                                                        aaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcagc                                                                  <  2:2818880/140‑1 (MQ=255)
                                                                           gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                                                               <  2:1766275/140‑1 (MQ=255)
                                                                           gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                                                               <  2:2763040/140‑1 (MQ=255)
                                                                           gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                                                               <  2:411601/140‑1 (MQ=255)
                                                                               aGGATAAAAACCTGGAAGGCCCTGATATAAGGGAAGGGCTTTCAGCCATTCTGTCTGTTCGGAGTCCTGAGGAGCTTCTTCAATGTGAGGGACAAACAAATGGCACGCTTGGTCCGAGTGACGCGAGATCAGCAGtgtat                                                           >  2:1642648/1‑136 (MQ=255)
                                                                                  aTAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCg                                                        >  2:1740310/1‑140 (MQ=255)
                                                                                    aaaaaCCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGgt                                                      <  1:2743060/140‑1 (MQ=255)
                                                                                           tGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCa                                               >  2:1700758/1‑140 (MQ=255)
                                                                                                 gCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcag                                         <  1:1964706/140‑1 (MQ=255)
                                                                                                  cACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcagc                                        >  1:449744/1‑140 (MQ=255)
                                                                                                    cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt                                      >  1:2291677/1‑140 (MQ=255)
                                                                                                    cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt                                      >  2:2991531/1‑140 (MQ=255)
                                                                                                    cTGATATAAGGGAAGGGCTTTCAGCCATTATTTATGTTCGGATTCCTGAGGAGCTTCTTCAATATGAAGGACAAAAAAAAGGAAAGCTTGGTACTAGTCAAGCGAGAACACCAGATGATGCGGTTGTTTCAGTGCAgctt                                      >  1:1737881/1‑140 (MQ=255)
                                                                                                    cTGATATAAGGGAAGGGCGTTCAGCCATTATTTCTGTTCGGATTCCTGATGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt                                      >  1:1367737/1‑140 (MQ=255)
                                                                                                       atatAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgcttgct                                   >  1:107194/1‑140 (MQ=255)
                                                                                                       atatAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgcttgct                                   >  1:2073599/1‑140 (MQ=255)
                                                                                                            agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGTAGGACAAACGAAAGGAAAGCTTGGTCCTAGTGAAGCGAGATCAGCAGTTGATGCGGGTGTGTCAGAGCAgcgtgctgagtt                              >  2:2258981/1‑135 (MQ=255)
                                                                                                            agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:1485036/1‑140 (MQ=255)
                                                                                                            agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:1395102/1‑140 (MQ=255)
                                                                                                            agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:2339221/1‑140 (MQ=255)
                                                                                                            agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAACTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:2339351/1‑140 (MQ=255)
                                                                                                                 agggGTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGACGGACAAACACAAGGAAAGGGTGGTAGTCGTGGAGCGCGTTCAGCAGATGGGGCGGGTGTTTCGGAGCCGCTGGCGTACTGTGTag                         >  2:2059914/1‑140 (MQ=255)
                                                                                                                 agggCTTTCGGCCATTATTTCTGTTCGGATTCCTGAGGCGCTTCTTCAATTTGAAGGACAAACAAAAGGACAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATTCGGTTGTTTCAGAGCAGATTGCTTAATGTTTag                         >  2:798392/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGCTTCCTGAGGAGCTTCTTCAATTTGAAGGACACACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGAGGGTGTTTCAGGGCAGCTTGCTTACTTTTTag                         >  2:1575386/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:2384694/1‑75 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  1:2317449/75‑1 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  1:2384694/75‑1 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGgt                                                      >  2:2173028/1‑111 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGgt                                                      <  1:2173028/111‑1 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTCCTGTTTag                         >  2:120046/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  1:2167638/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  1:617790/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:2452516/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:552665/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:3077503/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTGTTTag                         >  2:873092/1‑140 (MQ=255)
                                                                                                                 agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTAGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:874645/1‑140 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:164402/1‑140 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  2:314961/1‑140 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:269598/1‑140 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:2864620/1‑140 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTATTTata                        >  2:148643/1‑138 (MQ=255)
                                                                                                                  gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGATTGCTTACTTTTTaga                        >  2:309310/1‑140 (MQ=255)
                                                                                                                    gCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAgg                      <  1:2452516/140‑1 (MQ=255)
                                                                                                                    gCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTAGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAgg                      <  1:874645/140‑1 (MQ=255)
                                                                                                                      tttCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGaa                    <  2:2234998/140‑1 (MQ=255)
                                                                                                                          aGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGACAAcc                >  1:2308107/1‑140 (MQ=255)
                                                                                                                           gCCATTATTTCTGTTCGGATTCCTGATGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGAAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCg               <  1:1309337/140‑1 (MQ=255)
                                                                                                                              attattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  2:1764361/1‑140 (MQ=255)
                                                                                                                              attattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  2:2297777/1‑140 (MQ=255)
                                                                                                                                tattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATa          <  2:2556386/140‑1 (MQ=255)
                                                                                                                                 attTCTGTTCGGATTCCAGAGGAGCTTCTTCAATTTGCAGGACCAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATAc         <  2:343240/140‑1 (MQ=255)
                                                                                                                                      tGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCaa    <  2:262038/140‑1 (MQ=255)
                                                                                                                                       gTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAAc   >  2:2311070/1‑140 (MQ=255)
                                                                                                                                       gTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAAc   >  2:2308082/1‑140 (MQ=255)
                                                                                                                                        ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGTAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACg  >  1:2339314/1‑140 (MQ=255)
                                                                                                                                        ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            <  2:1190481/130‑1 (MQ=255)
                                                                                                                                        ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  1:1190481/1‑130 (MQ=255)
                                                                                                                                         |                                                                                                                                          
ACTCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACG  >  NZ_CP034943/1898190‑1898465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: