Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,898,330 G→C 5.6% R327R (CGG→CGC parE → DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,898,3300GC 5.6% 515.7 / 17.5 160R327R (CGG→CGCparEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base G (92/59);  new base C (4/5);  total (96/64)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 160 total aligned reads displayed.

TCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACG  >  NZ_CP034943/1898192‑1898465
                                                                                                                                          |                                                                                                                                       
tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                                                        <  2:1573073/140‑1 (MQ=255)
tCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGa                                                                                                                                        <  2:155730/140‑1 (MQ=255)
 cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:1325836/140‑1 (MQ=255)
 cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:2951705/140‑1 (MQ=255)
 cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:2038006/140‑1 (MQ=255)
 cATGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAt                                                                                                                                       <  1:1877528/140‑1 (MQ=255)
  aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                                                      >  2:704458/1‑140 (MQ=255)
  aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                                                      >  2:52132/1‑140 (MQ=255)
  aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                                                      >  2:1871178/1‑140 (MQ=255)
  aTGAGTCAGGTGCAAAAACTGCGATGACGCGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAGAACCTTGAAGGCACCGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTCCGCAtt                                                                                                                                      >  2:1405442/1‑140 (MQ=255)
         aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAACGGATAAAAACCTGGAAGGAACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               >  2:955686/1‑140 (MQ=255)
         aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               >  2:798323/1‑140 (MQ=255)
         aGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTgagg                                                                                                                               <  1:308751/140‑1 (MQ=255)
                aaaaCAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGcttct                                                                                                                        >  2:40339/1‑140 (MQ=255)
                        gATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTg                                                                                                                <  1:2613606/140‑1 (MQ=255)
                         ctGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGa                                                                                                               <  2:2215517/139‑1 (MQ=255)
                             cacGTGCTTTTAATGAATATGCTCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  2:1285917/140‑1 (MQ=255)
                             cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  2:434074/140‑1 (MQ=255)
                             cacGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGa                                                                                                           <  1:2807102/140‑1 (MQ=255)
                             aacGTGCTTTTATTTAATATGCGCGTAAAGTTGTTTTGCTGTTAGAATAGGATAATAACCTGGATGGCTGTGATTTAAGGGAAGGGCTTTCAGCAATTATTTCTTTTCGGATTCCTGAGGAGCTTATTCAATTTGAAGGa                                                                                                           <  2:1510633/139‑1 (MQ=255)
                                 tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTTAAGGacaaa                                                                                                       >  1:1546195/1‑140 (MQ=255)
                                 tGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                                                                       >  2:1309337/1‑140 (MQ=255)
                                 tGCTTTTAATGAATATGCGCGAAAAGTGGCTTGGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGacaaa                                                                                                       <  2:2440801/140‑1 (MQ=255)
                                        aaTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAgg                                                                                                >  1:139639/1‑140 (MQ=255)
                                         aTGAATATGCGCGAACAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  1:3005284/140‑1 (MQ=255)
                                         aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  2:3001644/140‑1 (MQ=255)
                                         aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  1:1141572/140‑1 (MQ=255)
                                         aTGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCGTCTTCAATTTGAAGGACAAACAAAAGGa                                                                                               <  2:2537024/140‑1 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGTATAAATACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  2:1729870/140‑1 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCt                                                                                          >  2:2693808/1‑140 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  1:1028469/1‑140 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:114287/1‑140 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:450209/1‑140 (MQ=255)
                                              tatGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCAGTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:1231209/1‑140 (MQ=255)
                                              taggcgcGAAAAGTGGCTTTGCTGACAGAACAGGATAAAAACCTGGAAGGCACTGAGATAAGCGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  2:1742138/137‑1 (MQ=255)
                                                  cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGt                                                                                      >  2:2482000/1‑140 (MQ=255)
                                                  cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCGGAGGAGCTTCTTCATTTTGAAGGACAAACAAATGGCACGATTGGt                                                                                      >  2:2692295/1‑140 (MQ=255)
                                                  cgcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTGTCAGCCATTATTTCTGTTCGGATTCATGAGGAGCTTCTTCAATTTGAAGGACAAACCAAAGGAAAGCTTGGt                                                                                      >  2:558074/1‑140 (MQ=255)
                                                   gcgAAAAGTGGCTTTGCTGAAAGAAAAGGCTAAACACCTGGAAGGCACTGATATAAGGCAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTa                                                                                     <  2:537672/140‑1 (MQ=255)
                                                   gcgAAAAGTGGCTTTGCTGAAAGAAAAGGATAAACACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTa                                                                                     <  1:2626039/140‑1 (MQ=255)
                                                      aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                                                                  >  2:1912309/1‑140 (MQ=255)
                                                      aaaaGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTa                                                                                  >  2:2291491/1‑140 (MQ=255)
                                                             gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:1014778/140‑1 (MQ=255)
                                                             gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:1862183/140‑1 (MQ=255)
                                                             gCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGc                                                                           <  1:2864960/140‑1 (MQ=255)
                                                                   cTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATc                                                                     <  2:1462890/140‑1 (MQ=255)
                                                                     gaaagaaaAGGCTAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:2909248/140‑1 (MQ=255)
                                                                     gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:2357278/140‑1 (MQ=255)
                                                                     gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:479915/140‑1 (MQ=255)
                                                                     gaaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCGTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcag                                                                   <  2:2769316/140‑1 (MQ=255)
                                                                      aaagaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATcagc                                                                  <  2:2818880/140‑1 (MQ=255)
                                                                         gaaaAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGt                                                               <  2:411601/140‑1 (MQ=255)
                                                                             aGGATAAAAACCTGGAAGGCCCTGATATAAGGGAAGGGCTTTCAGCCATTCTGTCTGTTCGGAGTCCTGAGGAGCTTCTTCAATGTGAGGGACAAACAAATGGCACGCTTGGTCCGAGTGACGCGAGATCAGCAGtgtat                                                           >  2:1642648/1‑136 (MQ=255)
                                                                                               gCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcag                                         <  1:1964706/140‑1 (MQ=255)
                                                                                                cACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGagcagc                                        >  1:449744/1‑140 (MQ=255)
                                                                                                  cTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt                                      >  1:2291677/1‑140 (MQ=255)
                                                                                                  cTGATATAAGGGAAGGGCTTTCAGCCATTATTTATGTTCGGATTCCTGAGGAGCTTCTTCAATATGAAGGACAAAAAAAAGGAAAGCTTGGTACTAGTCAAGCGAGAACACCAGATGATGCGGTTGTTTCAGTGCAgctt                                      >  1:1737881/1‑140 (MQ=255)
                                                                                                  cTGATATAAGGGAAGGGCGTTCAGCCATTATTTCTGTTCGGATTCCTGATGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgctt                                      >  1:1367737/1‑140 (MQ=255)
                                                                                                     atatAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgcttgct                                   >  1:107194/1‑140 (MQ=255)
                                                                                                     atatAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAgcttgct                                   >  1:2073599/1‑140 (MQ=255)
                                                                                                          agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:1485036/1‑140 (MQ=255)
                                                                                                          agggaagggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACtt                              >  2:2339221/1‑140 (MQ=255)
                                                                                                               agggCTTTCGGCCATTATTTCTGTTCGGATTCCTGAGGCGCTTCTTCAATTTGAAGGACAAACAAAAGGACAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATTCGGTTGTTTCAGAGCAGATTGCTTAATGTTTag                         >  2:798392/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:2384694/1‑75 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          <  1:2317449/75‑1 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCt                                                                                          >  2:2317449/1‑75 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGgt                                                      <  1:2173028/111‑1 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:3077503/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  1:2167638/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:552665/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:1102240/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  1:617790/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  1:2974649/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTGag                         >  2:146529/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTGTTTag                         >  2:873092/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCACAGCAGCTTGCTTACTTTTTag                         >  2:1636987/1‑140 (MQ=255)
                                                                                                               agggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTAGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTag                         >  2:874645/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:164402/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:269598/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:2864620/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  1:2727685/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTaga                        >  2:314961/1‑140 (MQ=255)
                                                                                                                gggCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGATTGCTTACTTTTTaga                        >  2:309310/1‑140 (MQ=255)
                                                                                                                  gCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAgg                      <  1:2452516/140‑1 (MQ=255)
                                                                                                                  gCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTAGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAgg                      <  1:874645/140‑1 (MQ=255)
                                                                                                                    tttCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGaa                    <  2:2234998/140‑1 (MQ=255)
                                                                                                                        aGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGACAAcc                >  1:2308107/1‑140 (MQ=255)
                                                                                                                         gCCATTATTTCTGTTCGGATTCCTGATGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGAAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCg               <  1:1309337/140‑1 (MQ=255)
                                                                                                                            attattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  2:2101545/1‑140 (MQ=255)
                                                                                                                            attattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  2:2297777/1‑140 (MQ=255)
                                                                                                                            attattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  2:1764361/1‑140 (MQ=255)
                                                                                                                            aatattTCTGTTCGGATTCCTGAGGAGCTTAGTCAATTTTAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCTGTTTATGGGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            <  2:2462565/138‑1 (MQ=255)
                                                                                                                              tattTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATa          <  2:2556386/140‑1 (MQ=255)
                                                                                                                               attTCTGTTCGGATTCCAGAGGAGCTTCTTCAATTTGCAGGACCAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATAc         <  2:343240/140‑1 (MQ=255)
                                                                                                                                    tGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAACAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCaa    <  2:262038/140‑1 (MQ=255)
                                                                                                                                     gTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAAc   >  2:2308082/1‑140 (MQ=255)
                                                                                                                                      ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGTAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACg  >  1:2339314/1‑140 (MQ=255)
                                                                                                                                      ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            <  2:1190481/130‑1 (MQ=255)
                                                                                                                                      ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGa            >  1:1190481/1‑130 (MQ=255)
                                                                                                                                      ttCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACg  >  1:2341947/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
TCATGAGTCAGGTGCAAAAACAGCGATGACACGTGCTTTTAATGAATATGCGCGAAAAGTGGCTTTGCTGAAAGAAAAGGATAAAAACCTGGAAGGCACTGATATAAGGGAAGGGCTTTCAGCCATTATTTCTGTTCGGATTCCTGAGGAGCTTCTTCAATTTGAAGGACAAACAAAAGGAAAGCTTGGTACTAGTGAAGCGAGATCAGCAGTTGATGCGGTTGTTTCAGAGCAGCTTGCTTACTTTTTAGAGGAAAACCGGGATACTGCAACG  >  NZ_CP034943/1898192‑1898465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: