Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,073,402 G→T 6.9% A297A (GCC→GCA EO946_RS10385 ← NTTRR‑F1 domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,073,4020GT 6.9% 305.2 / 6.3 110A297A (GCC→GCAEO946_RS10385NTTRR‑F1 domain
Reads supporting (aligned to +/- strand):  ref base G (62/38);  new base T (3/6);  total (66/44)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGCT  >  NZ_CP034943/2073266‑2073540
                                                                                                                                        |                                                                                                                                          
 tgctcccgttgctccggttgcacctgttgctccagtTGCTCCAGTTGCTCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACcagtggctccagtggctc                                                                                                                                        <  2:472051/140‑1 (MQ=18)
   caccagttgatcccgttgcaccagttgatcccgttgCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAgtggctccagtggctcca                                                                                                                                      <  1:1485873/140‑1 (MQ=255)
   caccagttgatcccgttgcaccagttgatcccgttgCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAgtggctccagtggctcca                                                                                                                                      <  1:1485734/140‑1 (MQ=255)
                  ttgcaccagttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTgcg                                                                                                                       >  2:1068921/1‑140 (MQ=255)
                          gttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                               <  1:2168779/140‑1 (MQ=255)
                           ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTTGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTTGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                              <  1:2209776/140‑1 (MQ=255)
                           ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                              >  1:2579019/1‑140 (MQ=255)
                           ttgatcccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTcc                                                                                                              >  1:2183253/1‑140 (MQ=255)
                                cccgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGTGCCAGTTTCCCCGGt                                                                                                         <  1:548812/140‑1 (MQ=255)
                                  cgttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGTTCCTGTAGCTCCAGTTGCACCAGTTGCTCCAGTGGCTCCTGTTACTCCCGTTGCGCCCGTTTCCCCGGTTg                                                                                                       >  2:1590393/1‑140 (MQ=255)
                                    ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCa                                                                                                     >  1:1316824/1‑140 (MQ=255)
                                    ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCa                                                                                                     <  2:1316824/140‑1 (MQ=255)
                                    ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCa                                                                                                                    <  2:3094661/125‑1 (MQ=255)
                                    ttgcTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTAGGTCCTGTAGCTCCAGATGCACCAGTGGATCCAGTGGCTCCAGTTACTCCCGTTGAGCCCGTTGCCACGGTTGCa                                                                                                     >  2:639885/1‑140 (MQ=25)
                                         ccTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgt                                                                                                <  1:2663782/140‑1 (MQ=255)
                                           tGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttg                                                                                              >  1:1815441/1‑140 (MQ=255)
                                           tGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCAGGTTGCACCAgttg                                                                                              >  1:1815100/1‑140 (MQ=255)
                                            gttcaccccGTTGCTCCCGTGGCTCCCGGGGCTCCTTTTGCTCCCGTTGCTCCTGTTCCGCCTGTTGCTCCTGTTGCACCAGTGGCTCCAGTTGCTCCTGTTTCTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttga                                                                                             <  1:1027104/134‑1 (MQ=9)
                                             ttGATCCCGTTGCTCCCGTGGCACCAGTGGCTCGTGTTGTTCCCGTTGGGCCTGTTGGTCCTGTAGCTAGAGTTGCTCCAGTGGGTCCTGGGGCTGCAGTTAATCCCGTTGCGCCAGTTGCGCCGGTTGCGGCTgttgat                                                                                            >  2:1551839/1‑140 (MQ=21)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            <  1:2544817/140‑1 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            <  1:2862135/140‑1 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            >  2:2013432/1‑140 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            >  2:2013687/1‑140 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            <  1:2914188/140‑1 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            <  1:2123145/140‑1 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGGTGCACCAgttgat                                                                                            <  1:1991243/140‑1 (MQ=255)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGATACTAGCGAGGCGACAGTTTGCCCGGTTGCAGCAgttgat                                                                                            >  2:2036768/1‑140 (MQ=25)
                                             ttGATCCCGTTGCTCCCGTCGCACCAGGGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTTGCTCCAGTGGCTCCAGTTCCTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgat                                                                                            <  2:1118515/140‑1 (MQ=255)
                                               gATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcc                                                                                          >  2:1101141/1‑140 (MQ=255)
                                                 tCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccg                                                                                        >  1:1482378/1‑140 (MQ=255)
                                                  cccGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCGGTTGGTCCTGTAGCTCCAGTTGCACCCGTGGCTCCAGTGGCGCCCGTTACTCCCGTTGCGCCCGTTTCCCCGGTTGCACCCgttgatcgcgt                                                                                       >  2:740145/1‑140 (MQ=25)
                                                  cccGGTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGCACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgt                                                                                       <  1:744545/140‑1 (MQ=255)
                                                      ttGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgct                                                                                   >  1:2724765/1‑140 (MQ=255)
                                                      ttGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgct                                                                                   >  1:1677920/1‑140 (MQ=255)
                                                              gTCGCACCAGTGGCTCCTGTTGATCGCGTTGGACCTGTTGGTCCTGTACCTCCAGTAGCCCCAGTGCCTCCCCTCGGTCCATGTAATCCCGTTGCGCCAGTATCACCGGTTGCGCCCgttgctcagcttgctgctgttgg                                                                           >  1:408585/1‑139 (MQ=16)
                                                              gTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttga                                                                           >  1:2567124/1‑140 (MQ=255)
                                                              gTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttga                                                                           >  1:1142746/1‑140 (MQ=255)
                                                                      aGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgttcccgttgctcctgttgatcccgttg                                                                   >  1:2442487/1‑140 (MQ=255)
                                                                      aGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttg                                                                   >  1:687387/1‑140 (MQ=255)
                                                                        gtgCTCATGTTTCTCCCGTTGCTCCCGTTGCTCCAGTAGCTCCAGTTGCACCAGTTTCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttgct                                                                 <  2:1213771/138‑1 (MQ=14)
                                                                                 ttGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGTCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATcccgttgctcctgttgatcccgttgctcctgttgat                                                        <  1:1935334/140‑1 (MQ=255)
                                                                                 ttGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATcccgttgctcctgttgatcccgttgctcctgttgat                                                        <  1:1165879/140‑1 (MQ=255)
                                                                                      cccGTTGGACCTGTTGGTCCTGTAGCTCCCGTTGCACCAGTGGGTCCGGTGGGTCCAGTTACTCCCGTGGCGCCAGTTTCCCCGGGTGCACCAGTTGTTGCCGTtgctcctgttgctcgggttgctcctgttgatccggt                                                   >  1:847397/1‑140 (MQ=21)
                                                                                      cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGttgctcctgttgatcccgttgcgcctgttgagcccgg                                                   >  2:23553/1‑139 (MQ=255)
                                                                                      cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                                   >  1:1367652/1‑140 (MQ=255)
                                                                                      cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                                   >  1:2780018/1‑140 (MQ=255)
                                                                                      cccGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                                   <  2:1911840/140‑1 (MQ=255)
                                                                                      cccGTTGCACCGGTTGGTCCTGTAGCACCAGGGGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGGTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                                   <  2:2430107/140‑1 (MQ=18)
                                                                                      cccGGTGGACCCGTTGGTCCTGTAGCACCAGTTGCACCAGAGGCCCCAGTGGCTCCAGTTACTCCCGTGGCGCCAGGTTCCCCGCGTGCACCAGTTGATCCCGTtgctcctgttgatcccgttgctcctgttgatcccgt                                                   <  1:1088526/140‑1 (MQ=18)
                                                                                         gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                               <  1:1882714/77‑1 (MQ=255)
                                                                                         gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTc                                                                                                               >  2:1882714/1‑77 (MQ=255)
                                                                                         gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttg                                                                   <  2:1918615/121‑1 (MQ=255)
                                                                                         gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttgatcccgttg                                                                   >  1:1918615/1‑121 (MQ=255)
                                                                                         gTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGGTCCTGTTGATCCCGTTGCGCCTGTTGATCCCGTCGc                                                >  2:176166/1‑140 (MQ=255)
                                                                                           tGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCGGTTGATCCCGTCGCAc                                              >  1:1382475/1‑140 (MQ=255)
                                                                                           tGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCACCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCAc                                              >  1:793967/1‑140 (MQ=255)
                                                                                                  gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgt                                                                              <  1:38692/101‑1 (MQ=255)
                                                                                                  gTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgt                                                                              >  2:38692/1‑101 (MQ=255)
                                                                                                   ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                                      <  1:1101141/140‑1 (MQ=255)
                                                                                                   ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                                      >  1:2651329/1‑140 (MQ=255)
                                                                                                   ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGAt                                      >  1:1334778/1‑140 (MQ=255)
                                                                                                   ttGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCAGTTGCGCCAGATTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCAGTTGATCCCGTTGCTCCTGTTGATCCCGACGCACCTGTTGAt                                      >  2:2167904/1‑140 (MQ=255)
                                                                                                        ccTGTAGCTCCAGTTGCCCCAGTCGCTCCAGTGGCTCCAGTTAATCCCGTTGCGCCAGTTTCACCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGt                                 <  1:2656143/140‑1 (MQ=255)
                                                                                                          agttgctccggttgctccggttGCTCCAGTTGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTg                               <  1:391880/136‑1 (MQ=17)
                                                                                                           gTAGCTCCAGTTGCACCAGTGGCTCCAGTTACTCCCGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTACGCCGGTTGATCCCGTGGCACCTGTTGATCCGGTTgg                              >  2:901839/1‑140 (MQ=11)
                                                                                                           gTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCGGTTGATCCCGTTgg                              >  2:1919132/1‑140 (MQ=255)
                                                                                                            tcgCTCCAGTTGCACCCGTTGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             <  1:1280040/138‑1 (MQ=255)
                                                                                                            tAGCTCCCGTTGCACCAGTTGCTCCCGTTGCTCCAGTTTATCCCGTTGCTCCAGTGTCCCCGGGTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             <  1:3063667/140‑1 (MQ=18)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             >  1:803297/1‑140 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             <  2:2651329/140‑1 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             <  2:1893169/140‑1 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             >  2:1819560/1‑140 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             <  2:1334778/140‑1 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCGCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGa                             >  2:812757/1‑140 (MQ=255)
                                                                                                            tAGCTCCAGTTGCACCACTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTTATCCCGGTGCGCCGGTTGCTCCGGAGGCGCCTGTTGATCCGGGTggg                             >  2:3094600/1‑139 (MQ=14)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        <  2:1352086/140‑1 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:2099128/1‑140 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        <  1:1774640/140‑1 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        <  1:1068921/140‑1 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:1730608/1‑140 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:1698392/1‑140 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:2554030/1‑140 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:2612737/1‑140 (MQ=255)
                                                                                                                 ccAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCGGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGt                        >  1:280143/1‑140 (MQ=255)
                                                                                                                    gttgctcccgttgctcccGTTGCTCCAGGTACTCCCGTTGCGCCAGTTTCACCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTgg                     <  1:2300269/140‑1 (MQ=17)
                                                                                                                    gTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGttgatcccgttgctcctgttgatcccgttgctcctg                                                             <  2:588043/100‑1 (MQ=255)
                                                                                                                    gTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTgg                     >  2:1234308/1‑140 (MQ=255)
                                                                                                                    gTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTgg                     >  1:2326934/1‑140 (MQ=255)
                                                                                                                       gCACCCCTGGCTCCTGTGGCTCCGTTTTCTCCCCTTGCGACAGTTTCCCCGGTTGCTCCATTTTATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTcc                  <  1:591010/140‑1 (MQ=21)
                                                                                                                       gCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTcc                  <  2:1234411/140‑1 (MQ=255)
                                                                                                                         aCCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCAGTTGGACCGGTTGGTCCTg                >  2:1590520/1‑140 (MQ=255)
                                                                                                                             gtggctccagtggctccaGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAgttgatcccgttgctcctgttga                                                                           <  1:1525615/77‑1 (MQ=255)
                                                                                                                              tggctccagtggctccagGTCCTCCGGTTGCGCCAGTTTCCCCGGTTGCACCAGTAGATCCCGTTGCTCCTCGGCCTCCCCTTGCTCCGGTTTATCCCGGAGCACCAGTTGTTCCCGTAGGACCGGTTGCTCCTGTAgag           >  2:1480983/1‑138 (MQ=12)
                                                                                                                               ggctccagtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCGAGTTGATCGCGTCGCACCGGTAGATCCCGTTGGACCTGTTGGTCCTCTAGCTc          >  1:2858310/1‑140 (MQ=21)
                                                                                                                               ggctccagtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTc          >  1:1267229/1‑140 (MQ=255)
                                                                                                                                  tccagtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCGGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAg       >  1:1413905/1‑140 (MQ=255)
                                                                                                                                     agtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCAc                                                                                                    <  1:1023970/44‑1 (MQ=255)
                                                                                                                                     agtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCAc                                                                                                    >  2:1023970/1‑44 (MQ=255)
                                                                                                                                      gtggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGc   >  1:2583179/1‑140 (MQ=255)
                                                                                                                                       tggctccagtTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGCt  <  2:2431708/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TTGCACCAGTTGATCCCGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCCGTCGCACCAGTGGCTCCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTTGCACCAGTGGCTCCAGTGGCTCCAGTTACTCCCGTTGCGCCAGTTTCCCCGGTTGCACCAGTTGATCCCGTTGCTCCTGTTGATCCCGTTGCTCCTGTTGATCCCGTCGCACCTGTTGATCCCGTTGGACCTGTTGGTCCTGTAGCTCCAGTGGCT  >  NZ_CP034943/2073266‑2073540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: