BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,555	C→A	6.4%	V48L (GTA→TTA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,555	0	C	A	6.4%	403.5 / 3.2	171	V48L (GTA→TTA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base C (46/107); new base A (16/2); total (62/109)
Fisher's exact test for biased strand distribution p-value = 1.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTAAC  >  NZ_CP034943/2214416‑2214694

cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAATTTTTTGGTCACTCTGTTTAc                                                                                                                                             >  2:159190/1‑140 (MQ=255)

 tCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACa                                                                                                                                            <  2:1230841/140‑1 (MQ=255)

   cgTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTGACAAg                                                                                                                                          <  1:2917026/139‑1 (MQ=255)

   actttatGGATGTTTTTCGCTTTATTACGATACAAACCGATTGAGTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTACGGCCGTTTATATTTTTGAAATAATGTTTTGCTCACTCTGTTTACAAg                                                                                                                                          <  1:2277041/138‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                          <  1:1017732/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                          <  1:1230218/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                          <  1:2328811/140‑1 (MQ=255)

   aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAg                                                                                                                                          <  1:2203305/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTTGGATACATACCGATTGATTTAATATCCTGGTGAAGCTCTTCCAGGGAAACAGCCATATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:2126243/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:1052053/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  2:2306234/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:423065/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:2861636/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:220732/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:2151442/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:2046618/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:1746730/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:1973697/140‑1 (MQ=255)

     tttttGGATGTTTTTCGCTTTAGTCCGATACAAACCGAGTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGACTTCCGCCCGTTTATATTTTTGAAAGAAAGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  2:309038/140‑1 (MQ=255)

     tttgtGGATGTTTTTCGCTTTATGACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:2533917/140‑1 (MQ=255)

     tttgtGGATGTTTTTCGCATTCTGACGATACAACCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  2:1818636/140‑1 (MQ=255)

     tgtttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  2:68096/138‑1 (MQ=255)

     attttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTg                                                                                                                                        <  1:383711/139‑1 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTGAATAATGTTTTGGTCACTCGGTTTACAAGTGCATCAGTACa                                                                                                                              >  1:523434/1‑140 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGTAATAATGTTTTTGTCACTGGGTTTACCAGTGCTTCTGTAcc                                                                                                                              >  2:2577118/1‑139 (MQ=255)

               tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCAGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTGAGTTTACAACTGCATATGtccc                                                                                                                              >  2:1959647/1‑137 (MQ=255)

               tttttCGCTTAATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGCCCGTTTATATTTTTGATATAATGTTATGGTCACTCTGGTTACAAGTGCATCTGTACa                                                                                                                              >  2:3043053/1‑140 (MQ=255)

               ttatacGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                              <  2:1815206/135‑1 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                         <  2:2834409/140‑1 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                         <  2:787576/140‑1 (MQ=255)

                    cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTAGATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                                                         <  2:511963/140‑1 (MQ=255)

                      ctatattaCGATACAAACCGATTGATTTAATATCCTGCTGAATCTCTTCCCGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCa                                                                                                                       <  2:2307479/137‑1 (MQ=255)

                         tattaCGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATTTTTTTGAAATAAAGTGTTTGTGACTGTGTTTAAAAGTGCAACAGTACATGGCGCAGAt                                                                                                                    >  2:272620/1‑140 (MQ=255)

                                aTACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGa                                                                                                             <  2:173002/140‑1 (MQ=255)

                                aTACAAACCGATTGATTTAATATCCAGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGACGTTTATTTTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACTTTGCGCAGATAAAGCGa                                                                                                             <  2:1295320/140‑1 (MQ=255)

                                 tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTTCAAGTGCATCTGTACATTGGGCAGATAAAGCGAc                                                                                                            >  2:538868/1‑140 (MQ=255)

                                 tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATGGTTTGGTGAATCGGTTTACAAGTGCATATGTAACTTGCGCCGATTAAGCGAc                                                                                                            >  2:446708/1‑140 (MQ=255)

                                   cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTTGAATAATGTTTTTGTGACGCTGTTTACAAATGCAACTGTACATAGCGCACAGAAAACCACTg                                                                                                          >  1:2421/1‑140 (MQ=255)

                                   cAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTTAATAATTTTTTGGTGAATCTGTTTAAAAGTTCATATGTACATTTCGCCGATAAAGCGAcag                                                                                                          >  1:357467/1‑138 (MQ=255)

                                     aaCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCt                                                                                                        <  2:2184438/140‑1 (MQ=255)

                                      aCCGATTGATTTAATATCCTGATGAAGCTCTTCCAGCGAAACATCCAAATAGTCTTCCGGCCGTGTATATTTTTTAAATAATGGTTTGGTCACTCGGTTTACAAGTGCAGCTGTACATTGAGCACATAAAGCGACTGCTa                                                                                                       >  2:1407388/1‑140 (MQ=255)

                                         gattgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATTATGTTTTTGTCACTCTGTTTACAATTGCATATTTACATTTCGCAGATAAAGCGACTGCTACCa                                                                                                    >  2:3089624/1‑140 (MQ=255)

                                         gattgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCa                                                                                                    >  2:3088014/1‑140 (MQ=255)

                                          attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                                                   <  1:3088386/140‑1 (MQ=255)

                                          attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCAc                                                                                                   <  1:622533/140‑1 (MQ=255)

                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACCGCCAAATAGTCTTCCGGCCGTGTATATTTGTGAAATAATGTTTTGGTGACTCTGTTTACAATTGCATCTGTACATTGCGCCGATTAATCGCCTGCTACCCCt                                                                                                  >  2:2775493/1‑140 (MQ=255)

                                           ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAAAGTTTTGGTCACGCTGTCTACCAGTGCATCTGTAACATGCGCAGATAAAGCCACTGCTACCACt                                                                                                  >  2:1201786/1‑140 (MQ=255)

                                                ttAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTGAAATAATTTTTTTTTCACACTGTTTAAAAATGCATATGTACATTGCGCCGCTAAAGCCACTGCTAACACaaaata                                                                                             >  1:268252/1‑137 (MQ=255)

                                                  attatCCTGGTGACGCTCTTCCAGCGAAACAGCCAAATAGTCTTCGGGCCGTTTATTTTTTCGAATTAATGTTTTGGTCACTCTGTTTACACGTGCATCTGTCCATTGCGCAGTTAAAGCGACTGCTACAACTAATTCaa                                                                                           <  1:3077012/138‑1 (MQ=255)

                                                  aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTTGTCACTCTGTTTACAAGTGCAACTGTACATTTCGCAGATAAAGCGACTGCTACCACTAATTaaa                                                                                           >  2:2093001/1‑140 (MQ=255)

                                                  aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                                           <  1:2073283/140‑1 (MQ=255)

                                                  aaTATCCTGCTGAAGCTATTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTATGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                                                           <  2:617922/140‑1 (MQ=255)

                                                   atatCCGGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTGGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaaa                                                                                          <  1:446708/140‑1 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTAAAAAGGAtt                                                                                    >  2:1647241/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATACTGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGGCTGCTACCACTAATTCAAAAGGttt                                                                                    >  2:2229861/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTTTTTTGTCACTCGGTTTACAAGTGCCTCTTTACATTGCGCACATAAAGCGACTGCTACCACTAATTAAACATGAtt                                                                                    >  2:2736071/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                                                    >  2:2759244/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACa                                                                                                                              <  1:15052/98‑1 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                                                    >  2:302134/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACAATGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAtt                                                                                    >  2:910907/1‑140 (MQ=255)

                                                         tgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTTGTGACGCTGTTTAAAACTGCATCTGTAAATTTCGCAGATAAACCGACTGCTAACACTAATTAAAAAGGAtt                                                                                    >  1:2562889/1‑140 (MQ=255)

                                                             gAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATATTGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGaa                                                                                <  1:89146/140‑1 (MQ=255)

                                                               aGCTCTTCCAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGGACATTGCGCAGATAAAGCGACTGCGACCACTAATTCAAAAGGATTGGAATg                                                                              <  2:877979/140‑1 (MQ=255)

                                                                 ctctTCCAGCTAACAAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATTTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTTCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:625140/140‑1 (MQ=255)

                                                                 ctctTCCAGCGCAACAGCCAAATAGTCTACCGGCCGTGTAGATTTTTGAAATAATGATTGGGGCACTCTGTTTACAAGTGCAACTGAACATTGCGCAGATAAAGCGACTGCTACCACTAATACAAAAGGATTGGAATGaa                                                                            <  1:560655/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:1162762/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:2448623/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:2661203/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:1994164/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:1511529/140‑1 (MQ=255)

                                                                 ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTGTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                                                            <  1:645603/140‑1 (MQ=255)

                                                                 ctcgTCCAGCGAAACACCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATACAAAAGGATTGGAATGaa                                                                            <  1:2178035/140‑1 (MQ=255)

                                                                    ttCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGTCAATCTGTTTACAACTGCATATTTACATTTCGCATATAAAGCGACTGCTAACACTAAATAAAAAAGATTTGAATGAAACa                                                                         >  2:2553648/1‑140 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGTAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACAACTAATTAAAAAGGATTTGAATTTACCAg                                                                        >  2:761591/1‑140 (MQ=255)

                                                                     tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACGAg                                                                                                                                          <  1:879744/74‑1 (MQ=255)

                                                                              aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACActc                                                               >  2:2079418/1‑140 (MQ=255)

                                                                              aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACActc                                                               >  2:2927573/1‑140 (MQ=255)

                                                                              aCAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAAAGTTTTGGTCACACTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAAATCCAAAGGATTTGAATGAACCACTTCACActc                                                               >  2:1916300/1‑140 (MQ=255)

                                                                                aGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                                             <  2:2119278/140‑1 (MQ=255)

                                                                                aGCCAAATAGTCTTCCGGCCGTGTATATGTGTGAAATAATGTTTTGGTCACTCTGGTTACAAGTGCATCTGTACCTTGCGCAGATAAAGCGACTGCTACCACTAATTCCAAAGGATTGGAATGAACCAGTTCACACTCTg                                                             <  1:1580011/140‑1 (MQ=255)

                                                                                aGCCAAATAGTCTTCCGCCCGTTTATATTTTTCAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                                                             <  2:1575841/140‑1 (MQ=255)

                                                                                        cgacTTCCGGCCGTGGATATTCTTGAAATAATGTTTTGGTCACGCTGTTTACAAGTGCATCTGGACATTGCGCAGATAAAGCGACTGCGACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                                                     <  1:2136390/137‑1 (MQ=255)

                                                                                        aGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                                                     <  1:2754377/140‑1 (MQ=255)

                                                                                          tCTTCCGGCCGTTTATATTTTTTAAAAAAATTTTTTGTGACTCTGTTTAAAAGTGCAAATGTAAATTGCGCAGATAAATCGACTGCTACCACTAAATCAAAAGGATTGGAATTAAACAGTTAACACACTGCGTGTGGcaa                                                   >  1:1733772/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTTTATTTTTTTAATAAAGTTTTTGTCACGCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTTCTACCACTAATTAAAAAGGATTGGAATGAAACAGTTAACACACAGCGTGTGTAAACa                                                 >  2:1003769/1‑140 (MQ=255)

                                                                                            ttCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACGCTGGTTACAAGTGCATCTGTACCATGGGCAGATAAAGCGACTGCAAACACTAATTCAAAAGGATTGGAATGAACCAGATAACACTCTGCGTGTGGaaaaa                                                 >  2:2475446/1‑138 (MQ=255)

                                                                                            ttCCGGCCGTTTATATTATTGAAATAATGTTTTGGTCACACTGTTTACAAGTGCATCTGTACAATTCCCACATAAAGCGACTGCTACCACTAATTAAAAAGGATTTGAATGAACAAGTTAAAACTCTTCGTGTTGAAACa                                                 >  2:1730984/1‑140 (MQ=255)

                                                                                             tCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACAt                                                <  2:2562889/140‑1 (MQ=255)

                                                                                                      ttATATTTTTGAAATAATGTTTTGGTCACTCTTTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt                                       <  1:2093001/140‑1 (MQ=255)

                                                                                                                  cataatGTTGTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACa                           <  2:3050400/139‑1 (MQ=255)

                                                                                                                           ttgtccACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt                  <  1:1916300/135‑1 (MQ=255)

                                                                                                                           ttGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt                  <  1:2927573/140‑1 (MQ=255)

                                                                                                                           ttGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAAt                  <  1:3095887/140‑1 (MQ=255)

                                                                                                                                  ctctTTTTAAAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGCTTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGtttt           <  2:1638395/140‑1 (MQ=255)

                                                                                                                                  ctctGTTTACAAGTGCATCTGTAGATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGtttt           <  2:1162835/140‑1 (MQ=255)

                                                                                                                                  ctctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGtttt           <  2:401607/140‑1 (MQ=255)

                                                                                                                                  ctctGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGtttt           <  2:1766236/140‑1 (MQ=255)

                                                                                                                                       tttACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGAtt      >  1:3094653/1‑140 (MQ=255)

                                                                                                                                           cAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTAac  <  1:661915/140‑1 (MQ=255)

                                                                                                                                           cAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATACAATTTGTTTTAGATTTAac  <  1:1530169/140‑1 (MQ=255)

CTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGTCACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTATCTTGTCTAGACAGAATTCAATTTGTTTTAGATTTAAC  >  NZ_CP034943/2214416‑2214694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`