Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,219,522 A→T 5.8% T742T (ACT→ACA dinG ← ATP‑dependent DNA helicase DinG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,219,5220AT 5.8% 488.4 / 16.1 148T742T (ACT→ACAdinGATP‑dependent DNA helicase DinG
Reads supporting (aligned to +/- strand):  ref base A (80/58);  new base T (4/5);  total (85/63)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 155 total aligned reads displayed.

CTTGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCACCCAGTCCCAGTC  >  NZ_CP034943/2219383‑2219661
                                                                                                                                           |                                                                                                                                           
cTTGCGCCAAGAGGTGACAGCCGGAAGCACACACCAGGTGTTTGAGTTCCTGATGAACCATTTTCAGCCTATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCATTGCCATCAGTTCGATATAACGGGCa                                                                                                                                             <  2:30926/140‑1 (MQ=255)
 ttGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAg                                                                                                                                            >  1:1489058/1‑140 (MQ=255)
  tGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGt                                                                                                                                           >  1:1844872/1‑140 (MQ=255)
  tGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGt                                                                                                                                           >  1:2876271/1‑140 (MQ=255)
  tGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGt                                                                                                                                           >  2:2500429/1‑140 (MQ=255)
      ccAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATTTCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATcc                                                                                                                                       <  2:81877/140‑1 (MQ=255)
      ccAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATcc                                                                                                                                       <  1:1144727/140‑1 (MQ=255)
      ccAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATcc                                                                                                                                       <  1:936614/140‑1 (MQ=255)
                     cGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCt                                                                                                                        >  2:846106/1‑140 (MQ=255)
                     cGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCt                                                                                                                        >  1:1275801/1‑140 (MQ=255)
                     cGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCt                                                                                                                        >  1:1276089/1‑140 (MQ=255)
                     cGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGAGTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCt                                                                                                                        >  1:574402/1‑140 (MQ=255)
                     cGGAAGCACTCATCAGGGGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCGTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCt                                                                                                                        >  1:2906225/1‑140 (MQ=255)
                      ggAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCtt                                                                                                                       <  1:831390/140‑1 (MQ=255)
                             cTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGt                                                                                                                >  1:754865/1‑140 (MQ=255)
                                        tttGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGGCCCGTATCTTGgattg                                                                                                     >  2:2178358/1‑140 (MQ=255)
                                        tttGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGgattg                                                                                                     >  2:1011157/1‑140 (MQ=255)
                                        tttGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGgattg                                                                                                     >  1:1378087/1‑140 (MQ=255)
                                          tGAGTTGCTGATGAACCTTTTTCACCATATCATGTGATGTTAACTGCACAAGGATTTTAGGCTGTTTTTGCTTTGCGATCAGTTAGTTATAAGGGGCAGTATGCTGTATAAATTCAGGTTGCCCCGTATGTTGgattgat                                                                                                   >  2:1980496/1‑140 (MQ=255)
                                          tGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATcc                                                                                                                                       >  1:247293/1‑104 (MQ=255)
                                          tGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGgattgat                                                                                                   >  2:2181812/1‑140 (MQ=255)
                                               tCCTGGTGAACGTTTTTAAGCATATCATTGGATGTAAACAGCACAAGGATTTTAGTCTGTTTTTCATTTGGCTTCAGTTCGATATAACGGGCTGTATCCTGTATAATTTCATGTTGCCCTGTATCATGAATTGatttcat                                                                                              <  1:1797468/140‑1 (MQ=255)
                                               tCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGatttcat                                                                                              <  2:3033224/140‑1 (MQ=255)
                                               tCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGatttcat                                                                                              <  2:385702/140‑1 (MQ=255)
                                                ccTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGatttcatt                                                                                             >  2:15782/1‑140 (MQ=255)
                                                   gatgaACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCt                                                                                          >  1:2201952/1‑140 (MQ=255)
                                                   gatgaACCTTTTTCAGCATATCAGGGGATGTTAACAGCACAAGGATTTTATGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGCCAGTCTCCTGTATAAATTCAGCTTGCCCCGGATCTTGGATTTATTTCATTTCt                                                                                          >  1:2313660/1‑140 (MQ=255)
                                                       aaCCTTTTTCAGCATATCATTGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTg                                                                                      <  1:1593973/140‑1 (MQ=255)
                                                         acTTTTTCAGCAGATAATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGa                                                                                    <  2:677801/139‑1 (MQ=255)
                                                           tttttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAat                                                                                  >  2:55949/1‑140 (MQ=255)
                                                           tttttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGAGTGAGTTCATTTCTTTTGGAat                                                                                  >  2:1567681/1‑140 (MQ=255)
                                                           tttttCAGCATATCATGGGATGTAAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCGTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGTaag                                                                                  >  2:2784502/1‑139 (MQ=255)
                                                            ttttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAatc                                                                                 >  1:1708723/1‑140 (MQ=255)
                                                              ttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGaataat                                                                               >  2:2620010/1‑140 (MQ=255)
                                                              ttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAatcat                                                                               >  2:287246/1‑140 (MQ=255)
                                                              ttCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAatcat                                                                               <  2:2194753/140‑1 (MQ=255)
                                                                cAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAAtcatca                                                                             <  2:1844872/140‑1 (MQ=255)
                                                                cAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAAtcatca                                                                             <  1:520771/140‑1 (MQ=255)
                                                                cAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAAtcatca                                                                             <  2:2876271/140‑1 (MQ=255)
                                                                cAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAAtcatca                                                                             <  2:2292886/140‑1 (MQ=255)
                                                                cAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAAtcatca                                                                             <  1:1318219/140‑1 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCCGTATCCTGTCTAAATTCAGCTGGCCCCGTATCTTGGAGTGATTTCAGTTCTTTTGGAAGCGGCAccc                                                                          >  1:2649232/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  2:1420579/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  1:1968677/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  2:1980021/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  1:2952675/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  1:2921986/1‑140 (MQ=255)
                                                                   cATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCAccc                                                                          >  1:2112239/1‑140 (MQ=255)
                                                                      ataaTGGGTTGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCGATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCATCTTGCCCAGTATGTTGGATTGATTTCATTTCTTTTTGAATCATCACCCGCa                                                                       <  2:2747433/137‑1 (MQ=255)
                                                                      atCATTGGATGGTAACAGCACAAGGATTTGAGGCTGTTTGTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGAGTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  2:961498/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCCCAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  2:707798/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  1:1026902/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  2:754865/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  2:2862443/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  1:2024765/140‑1 (MQ=255)
                                                                      atCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  1:2879060/140‑1 (MQ=255)
                                                                             gATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCtt                                                                >  1:1606241/1‑140 (MQ=255)
                                                                             gATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCtt                                                                >  1:1988153/1‑140 (MQ=255)
                                                                             gATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCtt                                                                >  1:230872/1‑140 (MQ=255)
                                                                                 ttAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCg                                                            >  2:541876/1‑140 (MQ=255)
                                                                                  tAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGt                                                           <  2:505888/140‑1 (MQ=255)
                                                                                      aGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       <  1:1816180/124‑1 (MQ=255)
                                                                                      aGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCa                                                                       >  2:1816180/1‑124 (MQ=255)
                                                                                       gCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGaa                                                      >  1:1007106/1‑140 (MQ=255)
                                                                                       gCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGaa                                                      >  2:2383827/1‑140 (MQ=255)
                                                                                        cacaAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCTGTATCCTGTATAAATTCAGGTTGCCCTGTATCCTGAATTGATTTCATTTCTTTTGGAATCATCACCTGCATACGCTCATCGTAAGaaa                                                     >  1:3059006/1‑140 (MQ=255)
                                                                                        cacaAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGaaa                                                     >  1:2619661/1‑140 (MQ=255)
                                                                                               aTTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGa                                              <  2:2491555/140‑1 (MQ=255)
                                                                                                ttttAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGAc                                             <  1:2181812/140‑1 (MQ=255)
                                                                                                  ttAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTc                                           <  1:591192/140‑1 (MQ=255)
                                                                                                  ttAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTc                                           >  1:41412/1‑140 (MQ=255)
                                                                                                  ttAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTc                                           >  1:2225531/1‑140 (MQ=255)
                                                                                                  ttAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTc                                           >  1:2532867/1‑140 (MQ=255)
                                                                                                    aGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGa                                         <  1:2978560/140‑1 (MQ=255)
                                                                                                    aGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGATTATCGTAAGAAAAAGGCGACTCGa                                         <  1:386424/140‑1 (MQ=255)
                                                                                                        tGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCTGTATCCTGTATAAATTCAGGTTGCCCTGTATCCTGAATTGATTTCATTTCTTTTGGAATCATCACCTGCATACGCTCATCGTAAGAAAAGGGTGACTCAATtct                                     >  2:1930360/1‑140 (MQ=255)
                                                                                                        tGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTGATCGTAAGAAAATGGCGACTCGATtct                                     >  2:2859214/1‑140 (MQ=255)
                                                                                                        tGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGAATCCTGTATAAAATCAGCTTGCCCCGTATATTGGATTGATTTCATTACTTTTTGAATCATAACCCGCATGCGATTATCGTAAGAAAAAGGACACTAGATact                                     >  2:1944706/1‑140 (MQ=255)
                                                                                                         gTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGGAAGAAAAAGGCGACTCGATtctc                                    >  2:1706180/1‑140 (MQ=255)
                                                                                                          tttttCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCa                                   >  1:1451191/1‑140 (MQ=255)
                                                                                                                    gCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCgg                         >  1:2695711/1‑140 (MQ=255)
                                                                                                                    gCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCgg                         >  2:2425361/1‑140 (MQ=255)
                                                                                                                    gCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCgg                         >  1:608926/1‑140 (MQ=255)
                                                                                                                    gCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGAGTCTCAGTGTcggcgg                         >  1:2737546/1‑140 (MQ=255)
                                                                                                                              cGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCa               <  2:2971498/140‑1 (MQ=255)
                                                                                                                               gATATAACGGGCTCTATCCTGTATAAATTCAGCTTGCCCCGGATCTTGGATTGATTTCATTGCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAc              <  2:1751081/140‑1 (MQ=255)
                                                                                                                               gATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAc              <  1:1354329/140‑1 (MQ=255)
                                                                                                                               gATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACCCGATTCTCATTGTCCGCGGGAAAAAGTCAc              <  2:2033819/140‑1 (MQ=255)
                                                                                                                                atatAACGGGCTGTATCCTGTATAAATTCAGGTTGCCCTGTATCCTGAATTGATTTCATTTCTTTTGGAATCATCACCTGCATACGCTCATCGTAAGAAAAGGGTGACTCAATTCTCATTGTCCGCGGGAAAAAGTCAct             <  1:2412157/140‑2 (MQ=255)
                                                                                                                                atatAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcc             <  1:840476/140‑1 (MQ=255)
                                                                                                                                atatAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcc             <  1:2976509/140‑1 (MQ=255)
                                                                                                                                atatAACGGGCAGTATCCAGTATAAATTCCGCTTGCCCCGTATCTTGGATTGTTTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcc             <  1:629918/140‑1 (MQ=255)
                                                                                                                                      cGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccagtcc       >  1:1216871/1‑140 (MQ=255)
                                                                                                                                      cGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccagtcc       >  1:1401695/1‑140 (MQ=255)
                                                                                                                                      cGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccagtcc       >  1:925035/1‑140 (MQ=255)
                                                                                                                                      cGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccagtcc       >  2:1073657/1‑140 (MQ=255)
                                                                                                                                           aGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGaaa                     >  1:549503/1‑121 (MQ=255)
                                                                                                                                           aGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccag          <  2:3040715/132‑1 (MQ=255)
                                                                                                                                           aGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCAcccag          >  1:3040715/1‑132 (MQ=255)
                                                                                                                                           aGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGAGTCTCATTGTCCGCGGGAAAAAGTCACccagtcccagtc  >  1:1321361/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTTGCGCCAAGAGCTGAATGCCGGAAGCACTCATCAGGTGTTTGAGTTCCTGATGAACCTTTTTCAGCATATCATGGGATGTTAACAGCACAAGGATTTTAGGCTGTTTTTCCTTTGCCATCAGTTCGATATAACGGGCAGTATCCTGTATAAATTCAGCTTGCCCCGTATCTTGGATTGATTTCATTTCTTTTGGAATCATCACCCGCATGCGCTTATCGTAAGAAAAAGGCGACTCGATTCTCATTGTCCGCGGGAAAAAGTCACCCAGTCCCAGTC  >  NZ_CP034943/2219383‑2219661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: