Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,452,637 C→T 6.3% S216S (TCG→TCA pstB ← phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,452,6370CT 6.3% 401.7 / 12.5 127S216S (TCG→TCApstBphosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base C (78/41);  new base T (4/4);  total (82/45)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 127 total aligned reads displayed.

TCCTCTGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACGTTGTTG  >  NZ_CP034943/2452500‑2452774
                                                                                                                                         |                                                                                                                                         
tCCTCTGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGa                                                                                                                                         >  2:3061068/1‑140 (MQ=255)
tCCTCTGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGa                                                                                                                                         >  2:1020271/1‑140 (MQ=255)
     tGTCTTTTGCTTTTTCGGACTCGTGAAAATCTGTTCGGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTAtt                                                                                                                                    >  2:122043/1‑140 (MQ=255)
     tGTCTTTTGCTTTTTCGGACTCGTGAAAATCTGTTCGGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTAtt                                                                                                                                    >  2:2234287/1‑140 (MQ=255)
     tGTCTTTTGCTTTTTCGGACTCGTGAAAATCTGTTCGGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTAtt                                                                                                                                    >  2:1534979/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACACGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTAtt                                                                                                                                    >  1:2574052/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAATAATGCCGTCCGGTCAGAAACCAGCAGAGCCTGCTGCATAGTGTGCGTGACAATAATAATCGAGGAtt                                                                                                                                    >  2:2838299/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTAtt                                                                                                                                    >  1:2208428/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTAtt                                                                                                                                    >  1:538229/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTAtt                                                                                                                                    >  2:2513731/1‑140 (MQ=255)
     tGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTAtt                                                                                                                                    >  2:2634518/1‑140 (MQ=255)
            tgctgcTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATCATAATCGAGTATTCACtttt                                                                                                                             >  2:1703616/1‑140 (MQ=255)
            tgctgcTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACtttt                                                                                                                             >  2:3013396/1‑140 (MQ=255)
            tgctgcTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACtttt                                                                                                                             >  1:1069255/1‑140 (MQ=255)
                 ctTCGGACTGGTGAAAATTTGTTCATTCTGTCCGAATTCTACAAGCTAGCCGTTTAAAAAGAATGCCGTCCGGACAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCAGTTTTTAGtt                                                                                                                        >  1:731397/1‑140 (MQ=255)
                 ctTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAAtt                                                                                                                        <  1:1808592/140‑1 (MQ=255)
                 ctTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAAtt                                                                                                                        <  1:1814008/140‑1 (MQ=255)
                 ctTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAAtt                                                                                                                        >  1:343163/1‑140 (MQ=255)
                           gTGAAAATTTGTTCAGTCTGTCCGTATTCTACACGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGtaattaa                                                                                                              >  2:904084/1‑140 (MQ=255)
                           gTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGAATATAGTGCTTCACAATAATTATCGAGTCTTCACTTTTTAATTCTGtaattaa                                                                                                              >  2:1553618/1‑140 (MQ=255)
                                 aTTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCctc                                                                                                        <  1:1543108/140‑1 (MQ=255)
                                 aTTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCctc                                                                                                        <  1:2524172/140‑1 (MQ=255)
                                                   tATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAAt                                                                                      >  2:2389527/1‑140 (MQ=255)
                                                    aTTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATc                                                                                     <  1:1020271/140‑1 (MQ=255)
                                                    aTTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATc                                                                                     <  1:3061068/140‑1 (MQ=255)
                                                     ttCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCg                                                                                    >  1:2614204/1‑140 (MQ=255)
                                                     ttCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCg                                                                                    >  1:1073023/1‑140 (MQ=255)
                                                         aCAAGCTCGCCGTTTAAAAATAATGCAGTCCGGTCATAAAACCGAAGAGCCTGATGCTTATTGTGCGTGACATTAATAATCGAGTATTCACTTTTTAATTATGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATc                                                                                <  2:1566032/140‑1 (MQ=255)
                                                         aCAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATc                                                                                <  2:710479/140‑1 (MQ=255)
                                                         aCAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATc                                                                                >  2:391357/1‑140 (MQ=255)
                                                         aCAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATc                                                                                <  2:2257796/140‑1 (MQ=255)
                                                         aCAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATc                                                                                >  2:3030994/1‑140 (MQ=255)
                                                         aCAACCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTGTGTAATTAATTCCTCGATTTTTGCATTTGAAATCGGAtg                                                                                >  2:59877/1‑139 (MQ=255)
                                                             gCTCGCCGTTTAAAAATAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTATGTAATTAATTCATCTATTTTTGCATTTGAAATAGGATCTAgc                                                                            >  1:913890/1‑140 (MQ=255)
                                                             gCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAgc                                                                            >  1:2794943/1‑140 (MQ=255)
                                                             gCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAgc                                                                            >  2:1060324/1‑140 (MQ=255)
                                                             gCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAgc                                                                            >  1:2520883/1‑140 (MQ=255)
                                                             gCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAgc                                                                            >  2:2894397/1‑140 (MQ=255)
                                                             gCTCGCCGTTTAAAAAGAATGCCGTCCGCTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGGATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCCTTTGAACTCGGATCTAgc                                                                            >  2:407676/1‑140 (MQ=255)
                                                                cGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTATCGCt                                                                         >  2:1367933/1‑140 (MQ=255)
                                                                cGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCt                                                                         >  1:684317/1‑140 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAACTCGGATCTAGCGCTGAAg                                                                     >  1:1691053/1‑140 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAg                                                                     <  2:892817/140‑1 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAg                                                                     >  1:617421/1‑140 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAg                                                                     <  2:2943725/140‑1 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAg                                                                     <  2:1372387/140‑1 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCGGAAg                                                                     <  2:775165/140‑1 (MQ=255)
                                                                    gTTTAAAAAGAATGCCGTCCGCTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAg                                                                     <  2:1933203/140‑1 (MQ=255)
                                                                               aTGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATcc                                                          >  2:444877/1‑140 (MQ=255)
                                                                               aTGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATcc                                                          >  1:1908649/1‑140 (MQ=255)
                                                                               aTGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATcc                                                          >  1:1843324/1‑140 (MQ=255)
                                                                                tGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACACTAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCca                                                         >  2:2657621/1‑140 (MQ=255)
                                                                                   cGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCcagca                                                      <  1:1389233/140‑1 (MQ=255)
                                                                                        ggTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCagaaga                                                 >  2:2207194/1‑140 (MQ=255)
                                                                                              aaaCCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAAcggac                                           >  2:539052/1‑138 (MQ=255)
                                                                                                 cccGCAGTGCCTGCTGCTTTTTGTGCTTTACAATAATAATCGAGTATTCACTTTTTAATTATGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTTGTTCATCCAGCAGAAGAAcggccggc                                        <  2:237722/140‑1 (MQ=255)
                                                                                                 cccGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAAcggccggc                                        <  2:2418835/140‑1 (MQ=255)
                                                                                                   cGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTATTCTCTTTTCAATCCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCAAGCGCTGAAGCTGGTTCATCAAGCAGAAGAACAGCCGGCtt                                      <  1:122043/140‑1 (MQ=255)
                                                                                                   cGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTATTCTCTTTTCAATCCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCAAGCGCTGAAGCTGGTTCATCAAGCAGAAGAACAGCCGGCtt                                      <  1:1534979/140‑1 (MQ=255)
                                                                                                   cGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATTGAGTATTCTCTTTTCAATCCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCAAGCGCTGAAGCTGGTTCATCAAGCAGAAGAACAGCCGGCtt                                      <  1:857804/140‑1 (MQ=255)
                                                                                                   cGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCtt                                      >  2:571859/1‑140 (MQ=255)
                                                                                                   cGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCtt                                      >  2:571774/1‑140 (MQ=255)
                                                                                                         gCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGc                                <  1:391357/140‑1 (MQ=255)
                                                                                                         gCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCAAGCAGAAGAACGGCCGGCTTCATCGc                                <  1:3030994/140‑1 (MQ=255)
                                                                                                          ccTGCTGCATCTTGTGCGTGACAATAATAATCGAGGATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCa                               >  2:2246507/1‑140 (MQ=255)
                                                                                                          ccTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCa                               >  2:3089865/1‑140 (MQ=255)
                                                                                                            tgctgcATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAg                             >  1:2778586/1‑140 (MQ=255)
                                                                                                               tgcATATTGTGCGTGACAATAATCATCGAGTATTCCCTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGt                          <  1:1341222/140‑1 (MQ=255)
                                                                                                                gcATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGACGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGtt                         <  1:256269/140‑1 (MQ=255)
                                                                                                                    atTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTcg                     <  2:1401268/140‑1 (MQ=255)
                                                                                                                    atTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCAGTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGACCGGCCGGCTTCATCGCACGCGTTCTcg                     >  2:1669331/1‑140 (MQ=255)
                                                                                                                     ttGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTcgc                    <  2:684317/140‑1 (MQ=255)
                                                                                                                      tgtgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTcgcg                   <  2:2652115/140‑1 (MQ=255)
                                                                                                                      tgtgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTcgcg                   <  2:2295416/140‑1 (MQ=255)
                                                                                                                      tgtgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTcgcg                   >  2:390585/1‑140 (MQ=255)
                                                                                                                      tgtgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTAATCCAGCAGAAGAACGGCCGGCTTCATCGCAATCGTTCTcgcg                   >  2:1043718/1‑140 (MQ=255)
                                                                                                                       gggCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGa                  <  2:741178/138‑1 (MQ=255)
                                                                                                                        tgCGTGCCAATAATAATCGAGTATTCACTGTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGACCGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:481208/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:2393414/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:1336163/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:1336798/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  2:2102641/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:2068448/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:3019153/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:480809/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:86469/1‑140 (MQ=255)
                                                                                                                        tgCGTGACAATAATAATCGAGGATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGAt                 >  1:980179/1‑140 (MQ=255)
                                                                                                                          cGTGAAAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATAc               <  1:2389527/140‑1 (MQ=255)
                                                                                                                           gTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACa              >  2:1365362/1‑140 (MQ=255)
                                                                                                                             gACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATa            >  1:1646442/1‑140 (MQ=255)
                                                                                                                             gACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATa            <  1:444877/140‑1 (MQ=255)
                                                                                                                             gACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATa            <  2:1843320/140‑1 (MQ=255)
                                                                                                                             gACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATa            <  2:1843324/140‑1 (MQ=255)
                                                                                                                             gACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCATCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAATCGGGTTCATCCAGCAGAAGAACGGCCGGCTACATCGCAAGCGTTCTCGCGATACATa            >  2:640913/1‑140 (MQ=255)
                                                                                                                              aCAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATaa           >  2:2686549/1‑140 (MQ=255)
                                                                                                                                  taataatCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACgt       <  1:159851/140‑1 (MQ=255)
                                                                                                                                  taataatCGAGTAGTCACTTTTTAATTCTGTACTTAATTCCTCTATTTGGGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACgt       <  1:484119/140‑1 (MQ=255)
                                                                                                                                       atCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACGttgttg  >  2:799637/1‑140 (MQ=255)
                                                                                                                                       atCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACGttgttg  >  2:808829/1‑140 (MQ=255)
                                                                                                                                       atCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACGttgttg  >  2:891099/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TCCTCTGTCTTTTGCTGCTTCGGACTGGTGAAAATTTGTTCAGTCTGTCCGTATTCTACAAGCTCGCCGTTTAAAAAGAATGCCGTCCGGTCAGAAACCCGCAGAGCCTGCTGCATATTGTGCGTGACAATAATAATCGAGTATTCACTTTTTAATTCTGTAATTAATTCCTCTATTTTTGCATTTGAAATCGGATCTAGCGCTGAAGCTGGTTCATCCAGCAGAAGAACGGCCGGCTTCATCGCAAGCGTTCTCGCGATACATAAACGTTGTTG  >  NZ_CP034943/2452500‑2452774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: