Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,278 C→G 14.8% E262Q (GAA→CAA)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2780CG14.8% 299.0 / 5.7 146E262Q (GAA→CAA) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base C (62/54);  new base G (21/2);  total (87/59)
Fisher's exact test for biased strand distribution p-value = 7.83e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

ATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGCGCGTCAAG  >  NZ_CP034943/2502145‑2502416
                                                                                                                                     |                                                                                                                                           
aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATAGAAAAACCCGGGTGTGGGGATTGAAAGCGCTTTAAAAAATCCTGTTGttttta                                                                                                                                      >  1:1287276/1‑139 (MQ=255)
aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGCGCTTTAAAACATCCTGTTCTTTTTc                                                                                                                                      >  2:1910983/1‑140 (MQ=255)
aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTc                                                                                                                                      >  2:283116/1‑140 (MQ=255)
    gCTTCTGATCAACGTAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                                                                  <  2:232596/140‑1 (MQ=255)
    gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCTATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCaagt                                                                                                                                  <  2:359855/140‑3 (MQ=255)
    gCTTATGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                                                                  <  2:3021268/140‑1 (MQ=255)
     cTTCTGATCAACGGAATAAGAATCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTTTTTCCAGAGTTTAATCGAAAACCCCGGGGGTGGTGGTTTCAAGTGCTTTAAAAAAACCTGTTGTTTTTCAAATa                                                                                                                                 >  1:1846314/1‑140 (MQ=255)
     cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGGGTGGGGGGCTTGTACGAGCTTTAAAAAATCCTGTTCTTTTTAAAATa                                                                                                                                 >  2:321768/1‑140 (MQ=255)
     cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCGTCATAGTCGTTTTGTTTCGCGATTTTAATCGAAATCGCCGTGGGTGGGGATTGAAGGCGGGTGTAAAAAGCCGGTTGTTTTTCAATTa                                                                                                                                 >  1:2193404/1‑140 (MQ=255)
     cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACAGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATATTAATCGAAATCCCCCTGTGTGGGGAGTGAACGAGCTTTAAAACATCCTGTTCTTATTGCAAtt                                                                                                                                  >  2:2388714/1‑140 (MQ=255)
        cTGATCATCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATg                                                                                                                              <  2:1801934/140‑1 (MQ=255)
        cTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATg                                                                                                                              <  2:120563/140‑1 (MQ=255)
        cTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATg                                                                                                                              <  2:434069/140‑1 (MQ=255)
           aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCGATCTCTTCATAGTCGTTTTGTTTACCCATTTTAAAAGAAAACACCGGGTGTGGGGCGTGGAGCAGCGTTAAAACAAACGGTTATTTTTCAATTAAttgtg                                                                                                                           >  1:555738/1‑136 (MQ=255)
             cAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATgagaga                                                                                                                         <  1:2041498/140‑1 (MQ=255)
               actcaaTAAGAAGCCGTAATAAGACTGCTTTTTTCCATATCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGTATTGAACGAGCTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAg                                                                                                                       <  2:555738/136‑1 (MQ=255)
               aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCGGGTTCTTTTTCAATTAATGAGAGTAg                                                                                                                       >  2:2381485/1‑140 (MQ=255)
               aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATTGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAg                                                                                                                       <  2:1039347/140‑1 (MQ=255)
               aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCACCGTGTGTGGGGATTTAACGAGCTTTAAAACATCCTGGTCTTTTTCAATTAATGAGAGAAg                                                                                                                       >  2:2840373/1‑140 (MQ=255)
               aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGTACGAGCTTTAAAACAACCGGTTCTTTTTCAATTAATGCGAGAAg                                                                                                                       >  2:104718/1‑140 (MQ=255)
                    aTAAGAAGCCGTAATCAGACTGCATTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGACAAGCGAcc                                                                                                                  <  1:276151/140‑1 (MQ=255)
                    aTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGAcc                                                                                                                  <  1:859544/140‑1 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACCAGCTTTAAAAAATCCTGTTCTTTTTAAATTAATGATAGTAGCGACCg                                                                                                                 >  1:2251680/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCgcacg                                                                                                                 >  2:1103616/1‑136 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGGTTCCCGCTTTTAATAGAAATCCCCGGGTGTGGGGCTTGTAAGGGCTTTAAAACATCCTGTTGTTTTTGAATTAATGAGAGAAGCGACCg                                                                                                                 >  1:497165/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                                                                 >  2:764579/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                                                                 >  2:509720/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAAACCCCGGGTGGGGGGATTTAACGAGCTTTTAAAAATCCTGTTGTTTTTGAATTAATGAGAGAAGCGACCg                                                                                                                 >  1:3032218/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACAAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCg                                                                                                                 >  2:913457/1‑140 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTACCCATTTTAATCGAAAAACCCGGGTGTGGGGATTGTACGTGCTTTAAAAAATACTGTTGATTTTGAAATAATGAGAGAAGCgcacg                                                                                                                 >  1:2533541/1‑136 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTAGTTACCGATTTTAATCGAAAAACCCGGGGGTTGGGATTTTACGAGCTTTAAAACATACTGTTGTGTTTAAATTAATTAGAGAAACGAccc                                                                                                                 >  2:337388/1‑139 (MQ=255)
                     tAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAATACCCGGGGGTGGGGATTTAACGAGCGTTAAAAAAAACTTGTGTTTTTGAATTAATGAGAGAAGCGACCg                                                                                                                 >  1:1139935/1‑140 (MQ=255)
                     tAACAAGCCTTAAGCAGACTGCAGTTGTCCATCTCATCATAGTCTTTTTCTTTCAATATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTTGACTTTTTCAATTAATGATACAAGCGACCg                                                                                                                 <  1:1269640/140‑1 (MQ=255)
                       agacgCCGTAATCAGACTGCTGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCGGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                                                                                               <  1:2047374/140‑1 (MQ=255)
                       agaagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGaa                                                                                                               <  1:1613363/140‑1 (MQ=255)
                           gCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAActct                                                                                                           >  2:2827347/1‑140 (MQ=255)
                             cGTAATCAGACTGCAGTTTTCCATCTCTTGATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAAGGAGAGAAGCGACCGAACTCTgg                                                                                                         <  2:43786/140‑1 (MQ=255)
                             cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATAGAAATCCCCGGGTGTGGGGATTTAACGAGCGTTAAAAAATCCGGTTGTTTTTGAATTAATGAGAGAAGCGACCCAACACTgg                                                                                                         >  1:378345/1‑140 (MQ=255)
                             cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTTATCGAAATCCCCGGGTGGGGGGATTGTACGAGCGTTTAAACATCCTGTTGTTTTTGAAATAATTTGAGAAGCGACCGCActaggg                                                                                                         >  1:2548323/1‑136 (MQ=255)
                             cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGGTTGAACGGGCGTTAAAACATCCTGTTCTTTTTAAATTAATTGGAGAAGCGACCGACCTCggg                                                                                                         >  1:3093953/1‑140 (MQ=255)
                             cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTATTTGCCCATTTTAATAGAAAAACCCGTGGGTGGGGGTTGAACGACCTTTAAAAAATACAGTTGTTTTTAAATTAATGAGAGAATCGACCGAACTCTgg                                                                                                         >  1:1885851/1‑140 (MQ=255)
                              gTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                                                                                        <  2:1661212/140‑1 (MQ=255)
                              gTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                                                                                        <  2:1657583/140‑1 (MQ=255)
                               tAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATTAGAGAAGCGACCGAACTCTGGTg                                                                                                       >  2:89584/1‑140 (MQ=255)
                                   ccagcTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAAGGAGAGAAGCGACCGAACTCTGGTGatat                                                                                                   <  1:779248/136‑1 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTTATAGAAAAACCCGTGTGTGGGGATTTTACGAGCTTTTAAAAATCCTGTTGTTGTTGAATTAATTTGAGAAGCGACCGAAATCTGGTGTTATAAt                                                                                                >  1:1091006/1‑140 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATCGAAATACCCGGGTGTGGGGTTTGAACGAGCTTTAAAAAAACCTGTTATTTTTGAATTAATGAGAGAAGCGACCGCACTCGGGTGATATCAt                                                                                                >  1:2650977/1‑140 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATCGAAAAACCCGTGTGGGGGGTTTTGACGAGGTTTTAAAAAACCGGTTATTTTTGAATTAATTAGAGAAGCGACCCAAACCGGGTGGTATAAt                                                                                                >  1:1213688/1‑140 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTGTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAt                                                                                                >  2:597878/1‑140 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGGTTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCGTTTAAAAATCCGGTTATTTTTTAATTAATTATAGAAGCGACCGAACTCTGGGGATATAAt                                                                                                >  1:1965259/1‑140 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTACCGGTTTTATAAGAAATACCCGTGTGTGGGGGTTTAAAGAGCTTTAAAAAATCCGGTTCTTTTTGAATTAATTTTATTAGAGAGCGACCTCTGGTGatataaa                                                                                                >  1:1054451/1‑137 (MQ=255)
                                      aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGCTTTTAATCGAAATCCCCGGGTGTGGGGATTGTACGAGCGTTAAAACTTCCGGTTCTTTTTCAATTAATGAGAGAAGCGGCCGAAATCTGGTGGTATCag                                                                                                >  1:741004/1‑139 (MQ=255)
                                          cAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATACCCCGGTGGGGGGATTGAACGAGCTTTAAAAAATACTGTTCTTTTTGAATTAATGAGAGAATCGACCGAACTCTGGGGGTATAATGGaa                                                                                            >  1:2329485/1‑140 (MQ=255)
                                                tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAACACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCgg                                                                                      <  1:1231697/140‑1 (MQ=255)
                                                 ccATCTGTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGTTTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                                                     <  1:102966/140‑1 (MQ=255)
                                                 ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                                                     <  1:764579/140‑1 (MQ=255)
                                                 ccATCTCTACATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                                                     <  2:2008844/140‑1 (MQ=255)
                                                  cATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGCTTGAACGAGCTTTAAAACATCCGGTTCTTTTTCAATTAATGAGAGAAGCGCCCGAACTCTGGTGATATCATGGAACTcccagt                                                                                    >  2:779248/1‑136 (MQ=255)
                                                  cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGatat                                                                                                   <  1:1936342/125‑1 (MQ=255)
                                                  cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGatat                                                                                                   >  2:1936342/1‑125 (MQ=255)
                                                     ctctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGCGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTggg                                                                                 >  2:2879383/1‑139 (MQ=255)
                                                       ctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGAACGAGCTTTAAAAAATCCAGTTCTTTTTCAAATAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTc                                                                               >  2:1708757/1‑140 (MQ=255)
                                                       ctTCATAGTCGTTTTCTTTACCGATTTTAAAAGAAAAACCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTGTTTTTCAATTAATTTGAGAAGCGACCGAACTCTGGTGATATAATGGAACTCCGGCTGGGTc                                                                               >  2:134270/1‑140 (MQ=255)
                                                       ctTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATCCCCCTGTGTGGGGCTTGTACGAGCTTTAAAACAACCTGTTCTTTTTAAATTAATGAGAGAAGCGACCGAACTCGGGTGATATAATGTAAAACCGGCTGGttg                                                                               >  2:24980/1‑139 (MQ=255)
                                                           aTAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCCGGGGTGGGGATTGTACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGATTAGCGACCGAACTCTGGTGATATGATTGAACTACGGCTGGTTGAGCa                                                                           >  1:3095637/1‑140 (MQ=255)
                                                            tAGTCGTTTTCGTTCCCGATTTTAATCGAAATCCCGGTGTGGGGGGTTTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAAGTCCGGCTGGTTCAGCat                                                                          <  1:2388714/140‑1 (MQ=255)
                                                                  tgttgtttcCCGATTTTAATCGAAAAACCCGTGGGTGGGGATTGAACGGGCTTTAAAACATCCTGTTGTTTTTCAATTAATGAGAGAACCGACCGAACTCTGGTGATATCATGGCACTGCGGGTGGTTCAGCATATTGAt                                                                    >  2:2345719/6‑140 (MQ=255)
                                                                                         aaTCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAAttttt                                             >  2:1856041/1‑140 (MQ=255)
                                                                                             cccGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTACTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAc                                         <  1:2345719/140‑1 (MQ=255)
                                                                                               cGTGTGTGGGGATTGAACGAGCTTTAACACATCCTGGTCATTTGCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt                                       <  1:2056385/140‑1 (MQ=255)
                                                                                                       gggATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTtgtg                               <  2:1684296/140‑1 (MQ=255)
                                                                                                            tGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGACGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg                          >  2:337658/1‑140 (MQ=255)
                                                                                                            tGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAATCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTGAACGTGATTTGTGGAATg                          >  1:2151572/1‑140 (MQ=255)
                                                                                                            tGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg                          >  1:3056831/1‑140 (MQ=255)
                                                                                                            tGAACGAGCTTTAAAACATCCTGTTATTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg                          <  1:1414308/140‑1 (MQ=255)
                                                                                                             gAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGa                         >  2:2185896/1‑140 (MQ=255)
                                                                                                              aaCGATCTTTAAAACATCCTTTTCTTTTTCAATTAAGGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGAATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGaa                        <  1:1910983/140‑1 (MQ=255)
                                                                                                              aaCGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGaa                        >  1:2474850/1‑140 (MQ=255)
                                                                                                              aaCGAGATTTAAAAAATCCTTTTTTTTTTAAATTAATGAGAGAAGCGACCGAACTCTGGTTATATCATGGAACTCCGGCTGGTTCAGCATATTTATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGaa                        <  1:249686/140‑1 (MQ=255)
                                                                                                               aCGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTTGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGaaa                       >  1:1867823/1‑140 (MQ=255)
                                                                                                               aCGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGaaa                       >  1:1701730/1‑140 (MQ=255)
                                                                                                                  aGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAATACAATTTTTCAACGTGATTTGTGGAATGAAAGGt                    >  1:485342/1‑140 (MQ=255)
                                                                                                                  aGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGt                    >  1:1355406/1‑140 (MQ=255)
                                                                                                                  aGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGt                    >  1:1583468/1‑140 (MQ=255)
                                                                                                                  aGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGt                    >  1:1035849/1‑140 (MQ=255)
                                                                                                                   gCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGtt                   >  2:2927795/1‑140 (MQ=255)
                                                                                                                    cTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTg                  >  1:1358575/1‑140 (MQ=255)
                                                                                                                         aaaaATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGa             <  1:104718/140‑1 (MQ=255)
                                                                                                                           aCATCCTTTTGTTTTTCAATTATTGTTAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTTCTACTAATTACTATTTTTCAAAGTGATTTGTGGATTGTAATGTTGatgtttt           <  1:337658/140‑7 (MQ=255)
                                                                                                                              tCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAgcgc        >  1:1647011/1‑140 (MQ=255)
                                                                                                                              tCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAgcgc        <  2:575282/140‑1 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg                          >  1:1050150/1‑121 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATg                          <  2:1050150/121‑1 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGcgcg       >  2:2898085/1‑140 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGcgcg       >  2:2519326/1‑140 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGAGTTGTGGAATGAAAGGTTGAACGAAGcgcg       >  2:1532768/1‑140 (MQ=255)
                                                                                                                               ccTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAAGGAACTCCGGCTGGGTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTTGAATGAAAGGTTGAACGAAGcgcg       >  2:1580872/1‑140 (MQ=255)
                                                                                                                                cTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGCGCGt      <  1:2835653/140‑1 (MQ=255)
                                                                                                                                cTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGCGCGt      <  2:1703171/140‑1 (MQ=255)
                                                                                                                                    tCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGCGCGTCAAg  <  2:2022846/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                           
ATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGTGATTTGTGGAATGAAAGGTTGAACGAAGCGCGTCAAG  >  NZ_CP034943/2502145‑2502416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: