Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 14.4% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA14.4% 433.7 / 14.1 213Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (102/72);  new base A (2/37);  total (104/109)
Fisher's exact test for biased strand distribution p-value = 1.40e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

TGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCA  >  NZ_CP034943/316126‑316399
                                                                                                                                        |                                                                                                                                         
tgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaaa                                                                                                                                        >  2:2359686/1‑140 (MQ=255)
  gAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATg                                                                                                                                      >  1:342287/1‑140 (MQ=255)
    aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCt                                                                                                                                    >  1:165961/1‑140 (MQ=255)
          cATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCaa                                                                                                                              >  1:1393337/1‑140 (MQ=255)
               gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                         >  1:1182770/1‑140 (MQ=255)
                tgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaaa                                                                                                                        <  2:1376073/140‑1 (MQ=255)
                tgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaaa                                                                                                                        <  2:3040651/140‑1 (MQ=255)
                tgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaaa                                                                                                                        <  2:520133/140‑1 (MQ=255)
                 gtTACGGTTCTAAGGATTGTGTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGCAACAAACAAAGAACGCAGTGCAAATGCTTGCCAACGGAAAg                                                                                                                       >  1:1592772/1‑140 (MQ=255)
                 gtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaagg                                                                                                                       >  1:869373/1‑138 (MQ=255)
                 gtTACGGGTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAACGCTGTTCAAATGCTTGCCAACGGagag                                                                                                                       >  2:3096498/1‑140 (MQ=255)
                  ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATGTAAATTTTTTGGGAAACAAACAAAGAAGGGAGTTCAAATGGTTGCCAACGGAAAGa                                                                                                                      >  2:1635291/1‑140 (MQ=255)
                    aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                    >  2:454094/1‑140 (MQ=255)
                       gTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaa                                                                                                                 >  2:2318290/1‑140 (MQ=255)
                            aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTATATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                            <  2:2164533/140‑1 (MQ=255)
                            aaGGATTGTTTAATATGGAGGCAAAATATGAGAGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                            <  1:2806876/140‑1 (MQ=255)
                            aaGGATTGTTTAATATGGAGCGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAACTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                            <  2:1138751/140‑1 (MQ=255)
                            aaGCATTCTTTAATATGGAGGGAAAATATGAGTCTAGAAGAGAAACCAATTAAGATAAAAGTCGAGAAACTATCTAAAAATTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                            <  1:2351195/140‑1 (MQ=255)
                                 ttttttAATTTGGAGGTAAAATATGATTGTAGATTAGAAACAAATTAAGATAAAAGTCGAGAACGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCATTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATcc                                                                                                       <  2:179976/137‑1 (MQ=255)
                                 ttGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACTGAACGACCAAAAAAGAGATcc                                                                                                       >  1:1242268/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTATATGAGAAACCAATTAAGATAAAAGTCGAGACAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGAAGTTCAAATGCTTGCAAACGGAAAGACAAAAAAAGAGATCCt                                                                                                      >  1:2061071/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTATGATCAAAGTCGAGAACGGATCGACAATTTTTGGGAAACGAACAAAGAAAGCTGTTCAAATGCTTGCCAACGGAAAGACACACAAAGAGATCCt                                                                                                      >  2:645651/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAATTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCCACGGAAAGACAAAAAAAGAGATCCt                                                                                                      >  2:535448/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCACCGGACAGACAAAAAAAGAGATCCt                                                                                                      >  1:2940523/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATcat                                                                                                      >  2:2527025/1‑138 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGAAGTTCAAATGCTTGCCAAAGGAAAGACAAAAAAAGATATCCt                                                                                                      >  2:1314880/1‑140 (MQ=255)
                                  tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTAGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCACACGGAAAGACAAAAAAACAGATCCt                                                                                                      >  2:2043523/1‑140 (MQ=255)
                                    tttAATATGGACGGAAAATATGAGATTAGATCACACACCAATAAAGATAAAAGTATAGAAAATATCTAAAATTTTTGGGAAACACACAAAGATAGAAGTTCAAATGGTTGCCAACGGAAAGAGAGTGAAAGAGATCCTGa                                                                                                    >  2:2116138/1‑140 (MQ=255)
                                         tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                               >  1:589069/1‑140 (MQ=255)
                                             gAGGGAAAATATGAGTGTAGATGAGGAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCg                                                                                           >  1:1035615/1‑140 (MQ=255)
                                             gAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACCAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGGAGATCCTGAAAGCGACCg                                                                                           >  1:1991616/1‑140 (MQ=255)
                                             gAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCg                                                                                           >  1:1966959/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:3000644/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:30289/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:1021783/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  2:1111635/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:1100121/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:1161411/1‑140 (MQ=255)
                                              aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                          >  1:1311856/1‑140 (MQ=255)
                                                     atatGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAATCAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGACAGCGACCGGAGCaagc                                                                                   >  2:2136968/1‑138 (MQ=255)
                                                        tGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTAAAATGCTTGCAAACGGAACGACAAAAAAAGAGATCCTGAAATCTACCGGATCAACCGtt                                                                                >  2:151019/1‑140 (MQ=255)
                                                            tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTCAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGCTgtcg                                                                            >  1:1869866/1‑137 (MQ=255)
                                                              tAGATGAGAAAGCACGTAAGATCAATGTCGAGAAAGTATCTAAAATTTGTGGGAAACAAACAAAGTATGCAGTTCAAATGCTTGCCAACGGAACGGCCAAAAAAGAGAGCCTGGAGGCGAGCGGATCAAGCGGTGGGGTa                                                                          >  2:1242825/1‑140 (MQ=255)
                                                              tAGATCAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGCAACAAACAAAGAAAGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTa                                                                          >  2:1860746/1‑140 (MQ=255)
                                                                gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaaa                                                                        >  1:2598703/1‑140 (MQ=255)
                                                                 aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                       >  2:1471024/1‑140 (MQ=255)
                                                                 aTGACAACCAAATTAAGAAAAACGTCGAGAAAGTATCTAAAATTTTTGGGAAAAAAACAAATAAAGCAGTTAAATTGCTGGCAAACGCAAACAAAAAAAAAGAGACCCTCAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                       <  1:753148/140‑1 (MQ=255)
                                                                 aTGACAAACCAATTAACAACAAACTCCAGAAAGTAACTAAAATTTTTGGCAAACAAACAACGAAACCAGTACAAAGCCTTCCCAACCGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                       <  1:3079149/140‑1 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:1894501/1‑140 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:2223071/1‑140 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:2483288/1‑140 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  1:2656396/1‑140 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTAAATTGATGGCCAACGGAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  <  2:707376/140‑1 (MQ=255)
                                                                      aaaCCAATTAAGATAAAAATCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                  >  2:746452/1‑140 (MQ=255)
                                                                      aaaCAAATTAACATAAAAGTCTAGAAAGTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCCGTACAAATGCTTGCCAACGGAAAGACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCACGc                                                                  <  2:475940/140‑1 (MQ=255)
                                                                           aaTTAATATAAAAGTCGATAAAGTATCTAAAATTTTTGGCAAAAAAAAAAATAAAGCAGTTAAAATGCTTGCAAACGTAAAGACAAAAAAAGAGATCCGGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtt                                                             <  2:2733725/140‑1 (MQ=255)
                                                                            aTTAAGATAAACGTCCCCAAAGTAACTAAAATTTTGGCGAAAAAAACAAAGAACGCAGTAAAAATGCTGGCCACCGCAAACAAAAAAAAACACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                            <  1:1082237/140‑1 (MQ=255)
                                                                            aTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                            <  2:2182863/140‑1 (MQ=255)
                                                                            aTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                            >  1:425052/1‑140 (MQ=255)
                                                                              tatgATAAAAGTCTATAAAGTATCTAAAATTTTTGGCAAACAAAAAAACAACCCTGTTAAACGGCTGGCCAACGCAAACACAAAAAAAGAGATCCTAAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  1:1751769/137‑1 (MQ=255)
                                                                              tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTAAACTGCTTGCCACCGGAAACCCAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:1039499/140‑1 (MQ=255)
                                                                              tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGCAAACAAAAAAATAAAGCAGTTAAAATGCTTGCCAACGCAAAGAAAAAAAAAGACATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:1191715/140‑1 (MQ=255)
                                                                              tAAGATAAAAGTCCACAAAGTAACTAAAATTTTTGGCAAACAAAAAAAGAAAGCAGTAAAAATGCTTCCCAACGCAAACAAAAAAAAAGACAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:1393337/140‑1 (MQ=255)
                                                                              tAAGAGAAAAGTCGATAAATTATATAAACTTTTTGGCAAACAAACAAACACAGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:2615032/140‑1 (MQ=255)
                                                                              tAAGAACAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                          <  2:1592772/140‑1 (MQ=255)
                                                                                ataaaaacGTCGATAAAGTCTATAAATTTTTTGCAAAACAAACAAACAAACCAGTACAACTGCTTGCCACCGGAAACAAAAAAAAACACAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  1:10976/136‑1 (MQ=255)
                                                                                ataaaaaaGTGGAGAACTTATCTAAAATTTTTGGGAAAAAAACAAAGAAAGCTGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1247457/136‑1 (MQ=255)
                                                                                ataTAAACGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAAAAAAGAACGCAGTCCAAATGCTTCCCAACGCAAACACAAAAAAATACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1966959/138‑1 (MQ=255)
                                                                                agctAAACGTCGAGAACGTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGGAAAGACAAAAAAGGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1991616/137‑1 (MQ=255)
                                                                                aGATAAATGTCGAGAAAGTATCTAAAATTTTTGGCAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:616110/140‑1 (MQ=255)
                                                                                aGATAAAATTCTTCAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCTGTTAAAATGTTTGCCACCGGAACGAAAAAAAAAGATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1773036/140‑1 (MQ=255)
                                                                                aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:342287/140‑1 (MQ=255)
                                                                                aGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGGAACCACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1568439/140‑1 (MQ=255)
                                                                                  aTAAAAGTCGAGAAAGTAGATAAAATTTTTGGCAAAAAAACAAAGAAAGCAGTACAAATGCTTGCCAACCGAAACACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGt                                                      <  2:1525924/140‑1 (MQ=255)
                                                                                    aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                                   <  2:2947688/125‑1 (MQ=255)
                                                                                    aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                    >  2:1410265/1‑140 (MQ=255)
                                                                                    aaaaGTCGAGAAAGTATCTAAAATTTTTGGCAAACAAACAAAGACAGCAGTTCAATTGCTTGCCACCGCAAAGACAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                    <  2:1980607/140‑1 (MQ=255)
                                                                                       aGTGGACAAATTATCTAAAATTTTTGGGAAAAAAACAATCAAACCCGTTAAAATGCTGGCCAACGCAAAGACAAAAAAAGATAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                 <  1:2900985/140‑1 (MQ=255)
                                                                                       aGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAAGGTAAATACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                 <  2:1083198/140‑1 (MQ=255)
                                                                                        ttccacaaaGTAAATAAATTTTTGGGCAAAAAAAAAAACAAAGAATTAAAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatga                                                <  1:362664/134‑1 (MQ=255)
                                                                                             gaAATTATCTAAAATTTTTGGGAAACAAAAAAATAAAGCATTTAAAATGCTTGCCAACGGAAAGAAAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                           <  2:1161411/140‑1 (MQ=255)
                                                                                               actaaccTAAAATTTTTGGCAAACAAACAAACAAAGCAGTTCAATGGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgag                                         <  1:2552243/134‑1 (MQ=255)
                                                                                               aaGTATAAAAAATTTTTGGGAAAAAAACAAAGAAAGCATTTAAATTGCTTGCCAACGTAAAGAAAAAAAAAGACTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgag                                         <  1:1073255/140‑1 (MQ=255)
                                                                                                  tATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                        <  2:1350894/122‑1 (MQ=255)
                                                                                                     cTAAAATTTTTGGTAAACAAACAAACAACGCATTTCAAATGCTGGCCAACGGAAACACAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                   <  1:1364460/140‑1 (MQ=255)
                                                                                                     atAAATTTTTTGGTAAACAAAAAAATAAAGCAGTTAAATTGCTTGCAAACGCAAACAAAAAAAAATACATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                   <  1:1523784/139‑1 (MQ=255)
                                                                                                     atAAATTTTTTGGCAAAAAAAAAAACAAACCAGTCAAAATGCTGCCCAACGCAAACAAAAAAAAATAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                   <  1:2728636/139‑1 (MQ=255)
                                                                                                       aaatTTTTTGGGAAAAAAACAAAGAAAGCTGTTAAAATGCTTCCCAACGCAAACACAAAAAAACATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                 <  1:547465/140‑1 (MQ=255)
                                                                                                       aaatTTTTTGGCAAACAACCAAAGAAAGCAGTTAAAAGCCTTGCCAACGCAAAAAAAAAAAAAGACAGCCGTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                 <  1:3112881/140‑1 (MQ=255)
                                                                                                           tttttGGGAAAAAAACAAAGAACGCAGTTAAAATGCTTGCCAACGTAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                             <  2:165961/140‑1 (MQ=255)
                                                                                                             tttGTGAAAAAAACATATAAACCAGTTAAACTGCTTGCCAACGGAAATAAAAAAAAAGATATCCGGAAAGCGACCGGATCTACCGTTGGTGTAAATCAAGCATATTTTGATGTATATGATGGTGAGCTATTTGTcatcat                           <  2:1729370/140‑1 (MQ=255)
                                                                                                             tttGGGAAAAAAACAAATAAAGCAGTTCAAATGGTTGCCAACGGAAAGACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:226415/140‑1 (MQ=255)
                                                                                                             tttGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:2839329/140‑1 (MQ=255)
                                                                                                             tttGGCAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGTAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:2598703/140‑1 (MQ=255)
                                                                                                             tgtGGTAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGCAACCACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                           <  2:1008170/138‑1 (MQ=255)
                                                                                                              ttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATg                          >  2:1025824/1‑140 (MQ=255)
                                                                                                               tGGGAAACAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                         <  2:324181/140‑1 (MQ=255)
                                                                                                                                     gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGACCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc   >  2:112451/1‑140 (MQ=255)
                                                                                                                                     gTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc   <  2:1870330/140‑1 (MQ=255)
                                                                                                                                      ttCAAATGCTTGCCAACGGACAGTCAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCa  >  1:2103648/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCA  >  NZ_CP034943/316126‑316399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: