Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,717,319 T→G 6.0% G399G (GGA→GGC sdhA ← succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,717,3190TG 6.0% 665.2 / 17.2 206G399G (GGA→GGCsdhAsuccinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (76/117);  new base G (7/6);  total (83/123)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TTCCGTCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGA  >  NZ_CP034943/2717183‑2717456
                                                                                                                                        |                                                                                                                                         
ttCCGTCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATtcct                                                                                                                                        >  2:2638881/1‑140 (MQ=255)
     tcaaTACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACAGAATTAGGTCCGGCCACCATTCCTCCGTa                                                                                                                                   >  1:3001593/4‑140 (MQ=255)
     tCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCgga                                                                                                                                   >  1:1990553/1‑138 (MQ=255)
     tCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTa                                                                                                                                   >  1:2340638/1‑140 (MQ=255)
     tCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTAGGTCCGGCCACCATTCCTCCGTa                                                                                                                                   >  2:866707/1‑140 (MQ=255)
       cATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTaaa                                                                                                                                 <  1:188390/140‑1 (MQ=255)
             cATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCgg                                                                                                                           <  2:2513863/140‑1 (MQ=255)
             cATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCgg                                                                                                                           <  1:2579581/140‑1 (MQ=255)
             cATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCgg                                                                                                                           <  1:2860956/140‑1 (MQ=255)
              aTAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                          >  2:1102609/1‑140 (MQ=255)
              aTAATCTCAGCCCATTTTTCGTCTTCTTTTGTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                          <  2:294197/140‑1 (MQ=255)
                     cAGCCCATTTTTCTGCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGACTTCTGCGGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAgg                                                                                                                   <  2:2727749/140‑1 (MQ=255)
                      atcccATTTTTCTTATTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCTTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAggt                                                                                                                  >  2:2302937/3‑139 (MQ=255)
                      aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                  >  1:2410847/1‑140 (MQ=255)
                      aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                  >  1:3069207/1‑140 (MQ=255)
                      aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                  >  1:1771225/1‑140 (MQ=255)
                      aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                  >  2:1806711/1‑140 (MQ=255)
                      aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                  >  2:2085581/1‑140 (MQ=255)
                        cccATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                          <  1:16353/130‑1 (MQ=255)
                        cccATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAg                                                                                                                >  1:372292/1‑140 (MQ=255)
                          cATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGtt                                                                                                              <  2:190743/140‑1 (MQ=255)
                                    cttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAg                                                                                                    >  2:1142705/1‑140 (MQ=255)
                                    cttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAg                                                                                                    >  2:2686566/1‑140 (MQ=255)
                                     ttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGc                                                                                                   >  2:1940549/1‑140 (MQ=255)
                                          ttttAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTg                                                                                              <  1:1348963/140‑1 (MQ=255)
                                              aaCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                          <  2:1028308/140‑1 (MQ=255)
                                               aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTCAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         <  1:2301625/140‑1 (MQ=255)
                                               aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         <  1:2916047/140‑1 (MQ=255)
                                               aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         <  1:1025584/140‑1 (MQ=255)
                                               aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         <  1:1438316/140‑1 (MQ=255)
                                                    aGCTTCGAATAAAGTTGAAGACATGTCTTCTGCTGAAGATTCTTAGCGTTTTACATATTTCCCCGCAGTCGTTCCGGCCACCCTTCCTCCGTAAATCCCGGAGAGCAGGTAGGTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                    <  2:771062/140‑1 (MQ=255)
                                                    aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                    <  1:2482212/140‑1 (MQ=255)
                                                    aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                    <  1:2638881/140‑1 (MQ=255)
                                                    aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                    <  1:447821/140‑1 (MQ=255)
                                                    aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                    <  1:1721496/140‑1 (MQ=255)
                                                     gCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCAt                                                                                   <  1:1834253/140‑1 (MQ=255)
                                                      cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                  <  1:1014609/140‑1 (MQ=255)
                                                      cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                  <  1:1678835/140‑1 (MQ=255)
                                                      cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                  <  1:980111/140‑1 (MQ=255)
                                                      cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                  <  1:2221138/140‑1 (MQ=255)
                                                           aaTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTa                                                                             <  2:2891220/140‑1 (MQ=255)
                                                            aTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTaa                                                                            <  1:417279/140‑1 (MQ=255)
                                                                 gATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATcaca                                                                       <  1:1534480/140‑1 (MQ=255)
                                                                 gATGAAGACATGTCTGCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTTCCCCCAAGCGGTTGCCGCCGTGCATTGAGTAATcaca                                                                       <  1:1867119/140‑1 (MQ=255)
                                                                  aTGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACAt                                                                      >  1:666067/1‑140 (MQ=255)
                                                                     aaGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTcc                                                                   >  2:378121/1‑140 (MQ=255)
                                                                      aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                  <  1:2588117/140‑1 (MQ=255)
                                                                      aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                  <  1:1153761/140‑1 (MQ=255)
                                                                         cATGTCGTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCgg                                                               <  2:757798/140‑1 (MQ=255)
                                                                            gTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTg                                                            >  2:3030125/1‑140 (MQ=255)
                                                                               ttctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaa                                                         <  1:514562/140‑1 (MQ=255)
                                                                                tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                        <  1:1102609/140‑1 (MQ=255)
                                                                                tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                        <  2:3077710/140‑1 (MQ=255)
                                                                                tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                        <  2:2838071/140‑1 (MQ=255)
                                                                                 ctgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                  <  2:1214513/113‑1 (MQ=255)
                                                                                  tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         <  2:1074091/105‑1 (MQ=255)
                                                                                  tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                         >  1:1074091/1‑105 (MQ=255)
                                                                                       aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                          >  2:1672917/1‑99 (MQ=255)
                                                                                       aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                 >  1:1330872/1‑140 (MQ=255)
                                                                                       aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                 >  1:1247685/1‑140 (MQ=255)
                                                                                       aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                 >  1:3013311/1‑140 (MQ=255)
                                                                                       aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                 >  1:887418/1‑140 (MQ=255)
                                                                                              tAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGt                                          >  2:428978/1‑140 (MQ=255)
                                                                                                     tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                  <  2:703121/109‑1 (MQ=255)
                                                                                                     tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                  >  1:703121/1‑109 (MQ=255)
                                                                                                     tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCACAGAGTCCCGGGATGTTTGTCAt                                   >  1:1693058/1‑140 (MQ=255)
                                                                                                     tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAt                                   >  1:2659537/1‑140 (MQ=255)
                                                                                                     tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGAGGTTTGTCAt                                   >  1:1550986/1‑140 (MQ=255)
                                                                                                      ttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAtt                                  <  2:139336/140‑1 (MQ=255)
                                                                                                      ttACATATTTCACCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAtt                                  <  2:2401165/140‑1 (MQ=255)
                                                                                                             tttCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGt                           >  2:612581/1‑140 (MQ=255)
                                                                                                               tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                         >  1:1926347/1‑140 (MQ=255)
                                                                                                               tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                         >  1:862333/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:2405223/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:2812031/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:928365/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:1505228/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:269156/1‑140 (MQ=255)
                                                                                                                cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                        >  1:984720/1‑140 (MQ=255)
                                                                                                                 cccGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:1768868/139‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:2226595/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:547690/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:468421/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  1:564756/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:3198/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:1330872/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:1629917/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:2037710/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  1:1409778/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  2:2293832/140‑1 (MQ=255)
                                                                                                                 aCCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                       <  1:2759494/140‑1 (MQ=255)
                                                                                                                        tCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCAt                >  1:3027982/1‑140 (MQ=255)
                                                                                                                             ccGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc           <  2:214415/140‑1 (MQ=255)
                                                                                                                             ccGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc           <  2:805411/140‑1 (MQ=255)
                                                                                                                                gCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGcc        >  1:323982/1‑140 (MQ=255)
                                                                                                                                  ctacaTTCCTCCTTAAATCGCGGAGATCAGGGTTTTTGCCCCCAAGCGGTTGCCTCCGTGCATATATTAATCAGATTCCCGGGATTCAAAGATTACCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCGCa      <  2:3001593/137‑1 (MQ=255)
                                                                                                                                  caccaTTCCTCCGTACAGCGCGGAGAGCAGGGAGTTTGCCCCCCAGCGGGTGCCGCCGTGCATAGAGTAATCCCATTCCCCGGCTGCATAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAACCCGCCCa      <  2:2052659/140‑1 (MQ=255)
                                                                                                                                  caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa      <  2:1990553/140‑1 (MQ=255)
                                                                                                                                  caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa      <  1:1998796/140‑1 (MQ=255)
                                                                                                                                      aTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGa  >  1:1597071/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTCCGTCCATACTCATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGA  >  NZ_CP034943/2717183‑2717456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: