Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,717,334 G→A 5.7% S394S (TCC→TCT sdhA ← succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,717,3340GA 5.7% 691.0 / 20.8 209S394S (TCC→TCTsdhAsuccinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (82/115);  new base A (7/5);  total (89/120)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 222 total aligned reads displayed.

CATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGA  >  NZ_CP034943/2717196‑2717473
                                                                                                                                          |                                                                                                                                           
cATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCgg                                                                                                                                            <  1:2594646/140‑1 (MQ=255)
cATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCgg                                                                                                                                            <  1:2861092/140‑1 (MQ=255)
 aTAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGCATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           <  2:783856/140‑1 (MQ=255)
 aTAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           >  2:1102609/1‑140 (MQ=255)
 aTAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           >  2:1301836/1‑140 (MQ=255)
 aTAATCTCAGCCCATTTTTCGTCTTCTTTTGTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           <  2:294197/140‑1 (MQ=255)
         aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGCGCAggg                                                                                                                                   >  1:1399633/1‑140 (MQ=255)
         aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:1806711/1‑140 (MQ=255)
         aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  1:1771225/1‑140 (MQ=255)
         aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:2410354/1‑140 (MQ=255)
         aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  1:2406760/1‑140 (MQ=255)
           cccATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           >  2:16353/1‑130 (MQ=255)
           cccATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGga                                                                                                                                           <  1:16353/130‑1 (MQ=255)
           cccATTTTTCTTCTTCTTTCTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAg                                                                                                                                 >  2:1998796/1‑140 (MQ=255)
           cccATTTTTCTTCTTCTTTCTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAg                                                                                                                                 >  2:2297415/1‑140 (MQ=255)
             cATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGtt                                                                                                                               <  2:190743/140‑1 (MQ=255)
                       cttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAg                                                                                                                     >  2:1142705/1‑140 (MQ=255)
                           ttttttAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGt                                                                                                                 <  2:301470/140‑1 (MQ=255)
                                 aaCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                                           <  2:1028308/140‑1 (MQ=255)
                                 aaCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATACGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                                           <  2:858446/140‑1 (MQ=255)
                                  aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTCAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:2301625/140‑1 (MQ=255)
                                  aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:1025584/140‑1 (MQ=255)
                                  aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:2011091/140‑1 (MQ=255)
                                  aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:1585130/140‑1 (MQ=255)
                                       aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                                     <  1:1721496/140‑1 (MQ=255)
                                       aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                                     <  1:2482212/140‑1 (MQ=255)
                                         cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:2221138/140‑1 (MQ=255)
                                         cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:1014609/140‑1 (MQ=255)
                                         cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:1678835/140‑1 (MQ=255)
                                              aaTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTa                                                                                              <  2:2891220/140‑1 (MQ=255)
                                               aTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTaa                                                                                             <  1:441959/140‑1 (MQ=255)
                                                    gATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATcaca                                                                                        <  1:1534480/140‑1 (MQ=255)
                                                    gATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATcaca                                                                                        <  1:3057492/140‑1 (MQ=255)
                                                     aTGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACAt                                                                                       >  1:666067/1‑140 (MQ=255)
                                                        aaGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTcc                                                                                    >  1:734743/1‑140 (MQ=255)
                                                        aaGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTcc                                                                                    >  2:378121/1‑140 (MQ=255)
                                                         aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCTAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                                   <  1:2376171/140‑1 (MQ=255)
                                                         aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                                   <  1:1153761/140‑1 (MQ=255)
                                                         aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                                   <  1:1419141/140‑1 (MQ=255)
                                                                  ttctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaa                                                                          <  1:1301836/140‑1 (MQ=255)
                                                                   tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  2:3077710/140‑1 (MQ=255)
                                                                   tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  1:1102609/140‑1 (MQ=255)
                                                                   tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  2:2838071/140‑1 (MQ=255)
                                                                     tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          >  1:1074091/1‑105 (MQ=255)
                                                                     tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  2:1074091/105‑1 (MQ=255)
                                                                          aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:1332909/1‑140 (MQ=255)
                                                                          aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:258012/1‑140 (MQ=255)
                                                                          aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:3013311/1‑140 (MQ=255)
                                                                                        tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                                           <  1:377310/85‑1 (MQ=255)
                                                                                        tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCACAGAGTCCCGGGATGTTTGTCAt                                                    >  1:1693058/1‑140 (MQ=255)
                                                                                        tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAt                                                    >  1:2659537/1‑140 (MQ=255)
                                                                                        tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGAGGTTTGTCAt                                                    >  1:1550986/1‑140 (MQ=255)
                                                                                         ttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAtt                                                   <  2:139336/140‑1 (MQ=255)
                                                                                              tatGTCACCGCATTCGGGCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATc                                              <  2:2276913/140‑1 (MQ=255)
                                                                                               atTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         >  2:257087/1‑112 (MQ=255)
                                                                                               atTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  1:257087/112‑1 (MQ=255)
                                                                                                tttCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGt                                            >  2:612581/1‑140 (MQ=255)
                                                                                                tttCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGt                                            >  2:2647626/1‑140 (MQ=255)
                                                                                                  tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                                          >  1:2919785/1‑140 (MQ=255)
                                                                                                  tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                                          >  1:1926347/1‑140 (MQ=255)
                                                                                                   cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:1505228/1‑140 (MQ=255)
                                                                                                   cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:269156/1‑140 (MQ=255)
                                                                                                   cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:2812031/1‑140 (MQ=255)
                                                                                                    cccGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1768868/139‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTTCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1332909/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:468421/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:547690/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2037824/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:207578/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:734743/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2186483/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  1:623862/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  1:2759494/140‑1 (MQ=255)
                                                                                                    aCCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1963120/140‑1 (MQ=255)
                                                                                                       gCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCAGGGATGTTTGTCATTTGATCTTAATCCAc                                     >  1:365236/1‑140 (MQ=255)
                                                                                                                ccGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc                            <  2:214415/140‑1 (MQ=255)
                                                                                                                acGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCCAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc                            <  2:764280/139‑1 (MQ=255)
                                                                                                                 cGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCg                           <  2:2697327/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:866707/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:984023/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:282174/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  2:3067659/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:891654/140‑1 (MQ=255)
                                                                                                                     caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:963505/140‑1 (MQ=255)
                                                                                                                     caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:1853993/140‑1 (MQ=255)
                                                                                                                     caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:55429/140‑1 (MQ=255)
                                                                                                                     caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:3070557/140‑1 (MQ=255)
                                                                                                                             ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCACTCGGTTGCCGCCGTGCAGAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTCTCATTTGATCGTAATCCACCCATAAACCGCCCATTGaataa               >  2:390522/1‑140 (MQ=255)
                                                                                                                             ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2870107/115‑1 (MQ=255)
                                                                                                                             ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        >  1:2870107/1‑115 (MQ=255)
                                                                                                                             ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCAGAAACCGCCCATTGaataa               >  2:2795330/1‑140 (MQ=255)
                                                                                                                                 gTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGa           <  1:1267804/140‑1 (MQ=255)
                                                                                                                                  tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATACACCGCCCATTGAATAATGGAc          >  2:1491870/1‑140 (MQ=255)
                                                                                                                                  tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGAc          >  2:313585/1‑140 (MQ=255)
                                                                                                                                  tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGAc          >  1:476592/1‑140 (MQ=255)
                                                                                                                                       cgcgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCg     >  1:19562/1‑140 (MQ=255)
                                                                                                                                       cgcgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCg     >  1:1887142/1‑140 (MQ=255)
                                                                                                                                         cgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCggg   <  2:1042928/140‑1 (MQ=255)
                                                                                                                                          ggAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATGCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGa  <  2:1711798/140‑1 (MQ=255)
                                                                                                                                          ggAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACAGTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGa  <  2:2313184/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CATAATCTCAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGA  >  NZ_CP034943/2717196‑2717473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: