Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,717,337 G→C 5.8% L393L (CTC→CTG sdhA ← succinate dehydrogenase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,717,3370GC 5.8% 681.6 / 25.4 208L393L (CTC→CTGsdhAsuccinate dehydrogenase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (81/115);  new base C (7/5);  total (88/120)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 208 total aligned reads displayed.

CAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGA  >  NZ_CP034943/2717204‑2717473
                                                                                                                                     |                                                                                                                                        
cAGCCCATTTTTCTGCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGACTTCTGCGGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAgg                                                                                                                                    <  2:2727749/140‑1 (MQ=255)
 atcccATTTTTCTTATTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCTTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAggt                                                                                                                                   >  2:2302937/3‑139 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCCTTCCTCCGTCACTCGCGGAGAGCAggg                                                                                                                                   >  1:1711798/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:1806711/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  1:2406760/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:1173293/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:2410354/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  2:2085581/1‑140 (MQ=255)
 aGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAggg                                                                                                                                   >  1:3069207/1‑140 (MQ=255)
   cccATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAg                                                                                                                                 >  1:372292/1‑140 (MQ=255)
   cccATTTTTCTTCTTCTTTCTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAg                                                                                                                                 >  2:3070557/1‑140 (MQ=255)
   cccATTTTTCTTCTTCTTTCTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAg                                                                                                                                 >  2:55429/1‑140 (MQ=255)
     cATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGtt                                                                                                                               <  2:190743/140‑1 (MQ=255)
               cttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAg                                                                                                                     >  2:2686566/1‑140 (MQ=255)
                ttcttTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGc                                                                                                                    >  2:1940549/1‑140 (MQ=255)
                   ttttttAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGt                                                                                                                 <  2:643767/140‑1 (MQ=255)
                     ttttAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTg                                                                                                               <  1:1348963/140‑1 (MQ=255)
                         aaCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGGAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                                           <  2:1028308/140‑1 (MQ=255)
                          aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTCAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:2301625/140‑1 (MQ=255)
                          aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:1438316/140‑1 (MQ=255)
                          aCGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  1:1025584/140‑1 (MQ=255)
                               aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                                     <  1:447821/140‑1 (MQ=255)
                               aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                                     <  1:2464326/140‑1 (MQ=255)
                               aGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCa                                                                                                     <  1:2638881/140‑1 (MQ=255)
                                 cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:2221138/140‑1 (MQ=255)
                                 cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:1681362/140‑1 (MQ=255)
                                 cGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATa                                                                                                   <  1:1014609/140‑1 (MQ=255)
                                      aaTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTa                                                                                              <  2:2891220/140‑1 (MQ=255)
                                       aTAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTaa                                                                                             <  1:417279/140‑1 (MQ=255)
                                          aaGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATca                                                                                          <  1:1553267/140‑1 (MQ=255)
                                            gATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATcaca                                                                                        <  1:1534480/140‑1 (MQ=255)
                                                aaGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTcc                                                                                    >  1:734743/1‑140 (MQ=255)
                                                aaGACATGTCTTCCGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGACCGGCCACCATGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTcc                                                                                    >  2:378121/1‑140 (MQ=255)
                                                 aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCTAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                                   <  1:2376171/140‑1 (MQ=255)
                                                 aGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTccc                                                                                   <  1:1153761/140‑1 (MQ=255)
                                                       gTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTg                                                                             >  2:3030125/1‑140 (MQ=255)
                                                           tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  2:1141458/140‑1 (MQ=255)
                                                           tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  1:1102609/140‑1 (MQ=255)
                                                           tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  1:1754860/140‑1 (MQ=255)
                                                           tctGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCaaa                                                                         <  2:2838071/140‑1 (MQ=255)
                                                             tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          >  1:1074091/1‑105 (MQ=255)
                                                             tgctgAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgcc                                                                                                          <  2:1074091/105‑1 (MQ=255)
                                                                  aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTgccgc                                                                                                           <  1:1672917/99‑1 (MQ=255)
                                                                  aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:3013311/1‑140 (MQ=255)
                                                                  aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:258012/1‑140 (MQ=255)
                                                                  aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:1247685/1‑140 (MQ=255)
                                                                  aaGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTccc                                                                  >  1:1330872/1‑140 (MQ=255)
                                                                                tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAt                                                    >  1:1806653/1‑140 (MQ=255)
                                                                                tttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAt                                                    >  1:2659537/1‑140 (MQ=255)
                                                                                 ttACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCAtt                                                   <  2:1393358/140‑1 (MQ=255)
                                                                                          tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                                          >  1:2919785/1‑140 (MQ=255)
                                                                                          tCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTaa                                          >  1:1926347/1‑140 (MQ=255)
                                                                                           cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:269156/1‑140 (MQ=255)
                                                                                           cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:2812031/1‑140 (MQ=255)
                                                                                           cACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAAt                                         >  1:1505228/1‑140 (MQ=255)
                                                                                            cccGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1768868/139‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1330872/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2037710/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:1629917/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2226595/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2293832/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  1:623862/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:3198/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:468421/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:734743/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  1:1409778/140‑1 (MQ=255)
                                                                                            aCCGCATTCGGTCCGGCCACCAGTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  1:2759494/140‑1 (MQ=255)
                                                                                            aCCGCAGTCGGTCCGGCCACCCTGCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACAGTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2946159/140‑1 (MQ=255)
                                                                                                   tCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCAt                                 >  1:3027982/1‑140 (MQ=255)
                                                                                                        ccGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc                            <  2:214415/140‑1 (MQ=255)
                                                                                                        ccGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc                            <  2:805411/140‑1 (MQ=255)
                                                                                                        acGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCCAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAAcc                            <  2:764280/139‑1 (MQ=255)
                                                                                                           gCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGcc                         >  1:323982/1‑140 (MQ=255)
                                                                                                           gCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGcc                         >  1:695474/1‑140 (MQ=255)
                                                                                                             caccaTTCCTCCGTACAGCGCGGAGAGCAGGGAGTTTGCCCCCCAGCGGGTGCCGCCGTGCATAGAGTAATCCCATTCCCCGGCTGCATAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAACCCGCCCa                       <  2:2052659/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:282174/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  2:1990553/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:866707/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:891654/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  2:2340638/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:963505/140‑1 (MQ=255)
                                                                                                             caccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:984023/140‑1 (MQ=255)
                                                                                                             caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:1998796/140‑1 (MQ=255)
                                                                                                             caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:55429/140‑1 (MQ=255)
                                                                                                             caccaTGCCTCCGTAAATCGCAGACAGCAGGGAGTTCGCTCCCAAGCGGTTGCCGCCGTGCATAGAATAATCACATTCCCCAGCCGCAAACAGTCCAGGGATATTTGTCATTTGATCGTAATCCACCCATAAACCGCCCa                       <  1:1853993/140‑1 (MQ=255)
                                                                                                               ccaTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGAGGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCAtt                     >  2:311853/1‑140 (MQ=255)
                                                                                                                 aTTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGa                   >  1:1597071/1‑140 (MQ=255)
                                                                                                                     ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCACTCGGTTGCCGCCGTGCAGAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTCTCATTTGATCGTAATCCACCCATAAACCGCCCATTGaataa               >  2:390522/1‑140 (MQ=255)
                                                                                                                     ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        <  2:2870107/115‑1 (MQ=255)
                                                                                                                     ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATc                                        >  1:2870107/1‑115 (MQ=255)
                                                                                                                     ctccGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCAGAAACCGCCCATTGaataa               >  2:2795330/1‑140 (MQ=255)
                                                                                                                         gTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGa           <  2:264330/140‑1 (MQ=255)
                                                                                                                          tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATACACCGCCCATTGAATAATGGAc          >  2:1491870/1‑140 (MQ=255)
                                                                                                                          tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGAc          >  1:476592/1‑140 (MQ=255)
                                                                                                                          tAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGAc          >  1:1293532/1‑140 (MQ=255)
                                                                                                                               cgcgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCg     >  1:19562/1‑140 (MQ=255)
                                                                                                                               cgcgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCg     >  1:1887142/1‑140 (MQ=255)
                                                                                                                                 cgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATCAACCGCCCATGGAATCATGGACTGCCggg   >  1:2553628/1‑140 (MQ=255)
                                                                                                                                 cgGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCggg   <  2:1042928/140‑1 (MQ=255)
                                                                                                                                  ggAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATGCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGa  <  2:1711798/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
CAGCCCATTTTTCTTCTTCTTTTTTAACGTGAGCGTCGAATAAAGATGAAGACATGTCTTCTGCTGAAGATTCTAAGCCGTTTACATATTTCACCGCATTCGGTCCGGCCACCATTCCTCCGTAAATCGCGGAGAGCAGGGAGTTTGCCCCCAAGCGGTTGCCGCCGTGCATAGAGTAATCACATTCCCCGGCTGCAAAGAGTCCCGGGATGTTTGTCATTTGATCGTAATCCACCCATAAACCGCCCATTGAATAATGGACTGCCGGGA  >  NZ_CP034943/2717204‑2717473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: