Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,729,937 C→A 8.6% E277* (GAA→TAA)  lcfA ← long‑chain‑fatty‑acid‑‑CoA ligase LcfA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,729,9370CA 8.6% 627.5 / 4.4 234E277* (GAA→TAA) lcfAlong‑chain‑fatty‑acid‑‑CoA ligase LcfA
Reads supporting (aligned to +/- strand):  ref base C (40/153);  new base A (30/2);  total (79/155)
Fisher's exact test for biased strand distribution p-value = 9.62e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

ACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCAAAAT  >  NZ_CP034943/2729799‑2730075
                                                                                                                                          |                                                                                                                                          
aCAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                           <  1:2988337/140‑1 (MQ=255)
aCAGATCATAATGCTGCAATGCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCg                                                                                                                                           <  1:2053507/140‑1 (MQ=255)
 cAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTTTTGAGAATATATTAACCGGTTACAGCGGAACAAACATAGGAACAAAAATAATTTcaa                                                                                                                                          >  2:1066120/1‑138 (MQ=255)
     tCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGcc                                                                                                                                      <  1:2334185/140‑1 (MQ=255)
        tAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTATGAATAAGAATCATTTGGAAGCCTAg                                                                                                                                   >  2:1244837/1‑140 (MQ=255)
         aaTGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGt                                                                                                                                  <  2:156214/140‑1 (MQ=255)
           tgctgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGGTGAATTTCAGCGGTTCGAGCGGATCCAACGTAGGAAGAAGAATCATTTAGAAGCCTAGttt                                                                                                                                >  2:783882/1‑140 (MQ=255)
           tgctgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTATGAAGAACAATCAATTAGCAGCCTAGgtt                                                                                                                                >  2:517565/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTTTTTGTGGATGATTTTGAGCGTTTCGAGCGGGTGAAAAATAGGAAGAAGAATCATTTCGAATCCGAGttgtat                                                                                                                             >  1:1045704/1‑136 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGGAGTTTTTAAGCGGTTGGAGGGGATCAAAATTAGGGAGAAGGATCATTTGGGAGGCTAGGTTact                                                                                                                             >  1:1589808/1‑138 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTTTGTGGATGATTTTGATCGGTTGGGGCGGTTCCAAAGTTGGAAGAAAAATCATTTAGTCGCCTAGTTTTAt                                                                                                                             >  1:1702755/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTCGATGTTTTTAAGCGTTTGGCGCGGATCAAACATAGGAAGAAGAATAATTTGTAAACCAAGTTTAAt                                                                                                                             >  1:1344545/1‑140 (MQ=255)
              tgcAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTGTCGAGTATTTTAAGCGGTTAGAGCGGAAAAAACTTAGGAATAAGAATCATTTATAAGCCTACTTTAAt                                                                                                                             >  2:1694945/1‑140 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTTTCGATTATTTTAAGCGTTTAGAGCGGATCAAACTTAGGAAGAAGAATAATTTAGAAACCTATTTTAAACg                                                                                                                           >  2:856645/1‑140 (MQ=255)
                cAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCg                                                                                                                           <  1:1351535/140‑1 (MQ=255)
                       gggTGCTGCACAAGGCCAAGCTAAAGTGGCGGGGCACCCGGAAAGAGACGCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                    <  1:2797717/140‑1 (MQ=255)
                       gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                    <  1:2913555/140‑1 (MQ=255)
                       gggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAAtt                                                                                                                    <  1:2648863/140‑1 (MQ=255)
                        ggTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGGTTTTGTTGTGGCTGATTTTGAGCGTTTGGAGCGGTGCCAACGTGGGACGAAGAATAATTTAGACGCCGAGTGTTATAGAAAAATtc                                                                                                                   >  1:2732244/1‑140 (MQ=255)
                         gTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtca                                                                                                                  <  2:1408278/140‑1 (MQ=255)
                           gATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtcatc                                                                                                                <  2:1869606/140‑1 (MQ=255)
                           gATGCAAAAGGCCAATGTAAATTGGCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATtcatc                                                                                                                <  2:1218106/140‑1 (MQ=255)
                             tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTGGATGATTTTAAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATAATTTAGAAGCCGAGATTAATCGAATAATTAATAAc                                                                                                              >  1:1729049/1‑140 (MQ=255)
                             tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCAc                                                                                                              <  1:834540/140‑1 (MQ=255)
                             tGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTGTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCAc                                                                                                              <  1:517320/140‑1 (MQ=255)
                                  aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  2:2289736/140‑1 (MQ=255)
                                  aaGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCg                                                                                                         <  2:2204785/140‑1 (MQ=255)
                                   aGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGt                                                                                                        <  2:1957929/140‑1 (MQ=255)
                                    ggCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTc                                                                                                       <  1:1224831/140‑1 (MQ=255)
                                    ggCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGGCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGCAGAAGAATCATTTAGAAGCCTAGTTTAATGGAATAATTCATCACTGCCGTc                                                                                                       <  1:1112738/140‑1 (MQ=255)
                                     gCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCa                                                                                                      <  1:2446310/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGTTGATTTTGAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTAAACACAGGCGTCAGt                                                                                                    >  2:2971709/1‑140 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGt                                                                                                    <  1:401488/140‑1 (MQ=255)
                                       cAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTCGATGATTTTAACCGTTTAGAGCGGATCAAACGTAGGAACAAGCATAATTTAGAAGCCTAGTTTAATCGAATAAATAATAACAGCCCTCact                                                                                                    >  2:1622907/1‑138 (MQ=255)
                                               aTTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTTTTTGTCGATGATATTCAGCCTTTCGATCGGATAAAACTTAGGAACAATAAAAATTTATAAACCAAGTTTAATCAAAAAATTAAAAAATGCCGTCAGTCCGTATAc                                                                                            >  2:694531/1‑140 (MQ=255)
                                               aTTGTCGGTGCACCCGGAAAGAGAGTCGGGTTTTGTTTTTCGGAGATTTTCAGCGTTTCGCGCGGATCAAACATAGGAAAAAAAAACAATTAAAAGCCAAGGTTAAACGAATAAATCAACACTTCCGTAAGTCAGtttaa                                                                                            >  2:145401/1‑139 (MQ=255)
                                                        gCACCCGGAAAGAGAGTCGGTTTTTTTTTTTCGATTATTTTGAGCGTTTCGAGCGGATAAAAATTATGAAGAAGAATAAATTAGAAACCGAGTTTAATCGAATAAATTATAACTGACGTCAGTCCGTATAAGTGaaaaaa                                                                                   >  2:2484803/1‑140 (MQ=255)
                                                               gAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACg                                                                            <  1:2387518/140‑1 (MQ=255)
                                                                    agagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGcc                                                                       <  2:2801924/140‑1 (MQ=255)
                                                                    agagTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGcc                                                                       <  2:1273005/140‑1 (MQ=255)
                                                                        tCGGTTTTTGTTTTTCGATTATTTTAAGCCTTTCGAGCCGATCAAACTTAGGAAGAAGAAAAAATTAGAAGCCAAGTTTAAAACAATAAATAAAAACTGCCCTCACTCCCTATACGTGAAAAAAACGGACCATGCCCAGc                                                                   >  2:21430/1‑140 (MQ=255)
                                                                        tCGGTTTTTGTTTTTCGATGATTTTAAGCGTTTAGAGCGGATAAAACATAGGAAGAACAAACATTTCGAAGCCGAGTTTAATAGAAAAATTAAACAAAGCCGTCAGTCCCTAAACGTGAAAAAACGGGACGATGCCCacc                                                                   >  2:1264902/1‑138 (MQ=255)
                                                                        tCGGTTTTTGTTTGTCGATTAATTTCAGCGTTTAGAGCGGATCAAAATTAGGAACAAGAATAATTTCGAACACTAGTTTAATCGAAAAAATCATCACAGCCGGAAGTCCGTATAAGTTAAAAAACCGGACGATTCCCacc                                                                   >  2:2514200/1‑138 (MQ=255)
                                                                          ggTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGAACAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTAAACGTGAAAAAACGGGACGATGCCCAGCAc                                                                 <  2:606836/140‑1 (MQ=255)
                                                                               ttgtttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACttttt                                                            <  1:2397982/140‑1 (MQ=255)
                                                                                  tttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAg                                                         <  1:246997/140‑1 (MQ=255)
                                                                                  tttgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAg                                                         <  1:2750585/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  2:136048/140‑1 (MQ=255)
                                                                                    tgtCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcc                                                       <  2:2027068/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCTAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  2:1812745/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  2:1468174/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  2:380682/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     >  2:1962045/1‑140 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  2:2473391/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  1:933785/140‑1 (MQ=255)
                                                                                      tCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGcccc                                                     <  1:1653492/140‑1 (MQ=255)
                                                                                         atgatTTTCAGCGTTTCGATCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttc                                                  <  2:2695055/140‑1 (MQ=255)
                                                                                         atgatTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCccttc                                                  <  2:2886267/140‑1 (MQ=255)
                                                                                              tttCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  2:840233/140‑1 (MQ=255)
                                                                                              tttCAGCGTTTCGACCGGATAAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATTATTCATCACTGCCGTCAGTCCGGATACGTGCAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTca                                             <  1:1030223/140‑1 (MQ=255)
                                                                                                tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                           <  2:1502816/140‑1 (MQ=255)
                                                                                                tCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTcaca                                           <  1:3006310/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  1:783882/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  2:1300969/140‑1 (MQ=255)
                                                                                                   gCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCg                                        <  1:21476/140‑1 (MQ=255)
                                                                                                      tttCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTAc                                     >  2:239137/1‑140 (MQ=255)
                                                                                                      tttCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTAc                                     >  2:431971/1‑140 (MQ=255)
                                                                                                       ttCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACa                                    >  2:2298307/1‑140 (MQ=255)
                                                                                                          gAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATcc                                 <  1:985948/140‑1 (MQ=255)
                                                                                                          gAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATcc                                 <  1:1439071/140‑1 (MQ=255)
                                                                                                             cGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAg                              <  1:2863600/140‑1 (MQ=255)
                                                                                                             cGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAg                              <  1:529300/140‑1 (MQ=255)
                                                                                                               gATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCg                            <  1:2222203/140‑1 (MQ=255)
                                                                                                                aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg                           >  1:1780282/1‑140 (MQ=255)
                                                                                                                aTCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCgg                           >  1:84364/1‑140 (MQ=255)
                                                                                                                 tCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGa                          >  2:557706/1‑140 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  2:646302/140‑1 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  1:947626/140‑1 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  2:2876868/140‑1 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  2:1625272/140‑1 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  1:1962045/140‑1 (MQ=255)
                                                                                                                  cAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAg                         <  1:2070756/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAATAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGATca                       <  1:1282139/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:3061757/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:1524352/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:2045812/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:2150295/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:386934/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  2:3060696/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:473689/140‑1 (MQ=255)
                                                                                                                    aaCTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGca                       <  1:1015792/140‑1 (MQ=255)
                                                                                                                         aGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACAttt                  <  2:804270/140‑1 (MQ=255)
                                                                                                                          ggAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                                 <  2:1980966/92‑1 (MQ=255)
                                                                                                                          ggAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCAc                                                                 >  1:1980966/1‑92 (MQ=255)
                                                                                                                               aagaaTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTAt            >  1:760083/1‑140 (MQ=255)
                                                                                                                                   aTCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGc        <  2:889760/140‑1 (MQ=255)
                                                                                                                                     cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa      <  1:431971/140‑1 (MQ=255)
                                                                                                                                     cATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCa      <  1:2485224/140‑1 (MQ=255)
                                                                                                                                       tttCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCCAGCGGAGCACATTTCAGTATTGGCCaaa    <  1:856556/140‑1 (MQ=255)
                                                                                                                                       tttCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCaaa    <  1:670150/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACAGATCATAATGCTGCAATTCGGGGTGATGCAAAAGGCCAATGTAAATTGTCGGTGCACCCGGAAAGAGAGTCGGTTTTTGTTTGTCGATGATTTTCAGCGTTTCGAGCGGATCAAACTTAGGAAGAAGAATCATTTCGAAGCCTAGTTTAATCGAATAATTCATCACTGCCGTCAGTCCGTATACGTGAAAAAACGGGACGATGCCCAGCACTTTTTCAGCCCCTTCCTTCACATCGTACATCCAAGCGGAGCACATTTCAGTATTGGCCAAAAT  >  NZ_CP034943/2729799‑2730075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: