Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,921,055 C→T 6.0% L59L (TTG→TTA ytrB ← ABC transporter ATP‑binding protein YtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,921,0550CT 6.0% 545.6 / 16.8 165L59L (TTG→TTAytrBABC transporter ATP‑binding protein YtrB
Reads supporting (aligned to +/- strand):  ref base C (87/68);  new base T (5/5);  total (92/73)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 170 total aligned reads displayed.

CATCAAATTTCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACT  >  NZ_CP034943/2920916‑2921187
                                                                                                                                           |                                                                                                                                    
caTCAAATTTCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACTCCATcaaac                                                                                                                                      >  1:1150338/1‑140 (MQ=255)
caTCAAATTTCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATcaaac                                                                                                                                      >  1:658804/1‑140 (MQ=255)
caTCAAATTTCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATcaaac                                                                                                                                      >  1:834799/1‑140 (MQ=255)
        ttCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAAAAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGt                                                                                                                              >  1:2577017/1‑140 (MQ=255)
                       cttAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGaa                                                                                                               >  1:124501/1‑140 (MQ=255)
                        ttAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGaaa                                                                                                              <  2:124501/140‑1 (MQ=255)
                        ttAGGATATCGACTAGCTGATTGTATCTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGaaa                                                                                                              <  2:2768432/140‑1 (MQ=255)
                          aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                            >  1:299716/1‑140 (MQ=255)
                          aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                            >  1:2104051/1‑140 (MQ=255)
                          aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                            >  1:2103950/1‑140 (MQ=255)
                               atCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAttt                                                                                                       >  1:1041692/1‑140 (MQ=255)
                                tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGAGCTTCGGATGCTTTTTGATTTCTCCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                      <  2:3003306/140‑1 (MQ=255)
                                tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                      <  1:1547252/140‑1 (MQ=255)
                                tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                      <  1:1690373/140‑1 (MQ=255)
                                tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                      <  1:2685591/140‑1 (MQ=255)
                                tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGAATGTAGATGATATTTTGTTTGCCCTTCGGATGCTTTTTGATTTATACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                      <  1:609255/140‑1 (MQ=255)
                                 cGACAAGCTGCTAGTATGTATACTTGTCGTAAAAAGTATTTTGAACAGGCATGTAGATGATATTTTGTTTGACATTCGGATGATATTTGATTTCTACGCCATCAAACAAAATAGTCCCGCTGTCGGCGAAAAAGAgttcc                                                                                                     >  2:2521939/1‑138 (MQ=255)
                                   aCTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTg                                                                                                   >  1:2537225/1‑140 (MQ=255)
                                       gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                               >  2:2450207/1‑140 (MQ=255)
                                       gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                               >  2:1118768/1‑140 (MQ=255)
                                       gctgctTGTATGTAACCTTGTCGTAAAAAGGAGTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTGCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                               <  1:2258304/140‑1 (MQ=255)
                                            ttGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                          <  1:616942/140‑1 (MQ=255)
                                            ttGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                          <  1:1946981/140‑1 (MQ=255)
                                            ttGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                          <  1:795444/140‑1 (MQ=255)
                                             tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                         >  1:1454732/1‑140 (MQ=255)
                                             tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                         >  1:2414467/1‑140 (MQ=255)
                                             tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                         >  1:520853/1‑140 (MQ=255)
                                             tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                         >  1:41932/1‑140 (MQ=255)
                                             tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                         >  2:132117/1‑140 (MQ=255)
                                                 tgtatACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCa                                                                                     <  1:1119211/140‑1 (MQ=255)
                                                   tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                   <  2:660155/140‑1 (MQ=255)
                                                    ataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTg                                                                                  <  2:2657905/140‑1 (MQ=255)
                                                     taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTc                                                                                                                     >  1:1075306/1‑104 (MQ=255)
                                                     taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                 >  2:2912789/1‑140 (MQ=255)
                                                     taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                 >  1:992087/1‑140 (MQ=255)
                                                     taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                 >  1:1455042/1‑140 (MQ=255)
                                                     taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                 >  1:1806449/1‑140 (MQ=255)
                                                        ttGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGt                                                                              <  2:2794453/140‑1 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAt                                                                                                         <  2:1200547/112‑1 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAt                                                                                                         >  1:1200547/1‑112 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             <  2:2447588/140‑1 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             >  2:1701694/1‑140 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             <  2:1241138/140‑1 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             >  1:1178813/1‑140 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             >  1:871937/1‑140 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             >  1:551655/1‑140 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                             <  1:3059692/140‑1 (MQ=255)
                                                         tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGtt                                                                             >  1:163675/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATCTTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCgatg                                                                            >  1:1022330/1‑137 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCtttt                                                                            >  1:2904814/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                            >  1:781276/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                            >  2:2495538/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                            >  1:2933435/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                            >  2:1399984/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGttt                                                                            >  1:856378/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTAAGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTTCTGGATTAAGCGAAGCATTGTCtttt                                                                            >  1:2763472/1‑140 (MQ=255)
                                                          gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATGTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTCAGCGAAGCATTGTCGtgt                                                                            >  1:2990140/1‑138 (MQ=255)
                                                           tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                           >  2:777740/1‑140 (MQ=255)
                                                           tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                           >  2:1777305/1‑140 (MQ=255)
                                                             gTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                         <  2:1521124/140‑1 (MQ=255)
                                                              tAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGcc                                                                        >  2:2622912/1‑140 (MQ=255)
                                                                                ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                      <  1:1701694/140‑1 (MQ=255)
                                                                                ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                      <  2:2577017/140‑1 (MQ=255)
                                                                                ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAGTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                      <  2:509228/140‑1 (MQ=255)
                                                                                ggCATGTAGATGATATTTTGTTTGACATTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                      <  1:1344925/140‑1 (MQ=255)
                                                                                 gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                     >  2:2669957/1‑140 (MQ=255)
                                                                                 gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                     >  2:1572287/1‑140 (MQ=255)
                                                                                       aGATGATTTTTTGTTTGACCTTCGGATGATTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                               <  2:643138/140‑1 (MQ=255)
                                                                                       aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                               <  2:1947379/140‑1 (MQ=255)
                                                                                       aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                               <  2:645916/140‑1 (MQ=255)
                                                                                           gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTTCGGCCGAGCCGCCCGAAGa                                           >  2:1528664/1‑140 (MQ=255)
                                                                                           gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                           >  2:916274/1‑140 (MQ=255)
                                                                                           gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                           >  1:2037131/1‑140 (MQ=255)
                                                                                            atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                          >  2:952221/1‑140 (MQ=255)
                                                                                            atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                          >  1:309475/1‑140 (MQ=255)
                                                                                                    tttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtt                                  >  1:1042770/1‑140 (MQ=255)
                                                                                                     ttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttt                                 <  2:2408797/140‑1 (MQ=255)
                                                                                                      tgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtttt                                >  2:1138849/1‑140 (MQ=255)
                                                                                                       gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                               <  2:2261615/140‑1 (MQ=255)
                                                                                                       gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                               <  2:1039370/140‑1 (MQ=255)
                                                                                                         ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                             <  2:1467567/140‑1 (MQ=255)
                                                                                                          cTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCaa                            <  2:2515553/140‑1 (MQ=255)
                                                                                                                  gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                    <  2:1150338/140‑1 (MQ=255)
                                                                                                                  gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                    <  2:3079729/140‑1 (MQ=255)
                                                                                                                  gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                    <  2:834799/140‑1 (MQ=255)
                                                                                                                    tttttGATTTCTACGCCATCAAACACAATCGTCCCGCTGTCGGCTAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGa                  <  2:535496/140‑1 (MQ=255)
                                                                                                                    tttttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGa                  >  2:1390766/1‑140 (MQ=255)
                                                                                                                    tttttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATGTCTCCTTTTTCAATTGTTAATGa                  >  2:2821178/1‑140 (MQ=255)
                                                                                                                      tttGATTTCTACGACATCAAACAAAATCGTCCCGCTGTCTGAGACAATGATTTGCTGTATTAAGCGAAGCATTGTCGTTTTGCATGACCCATTGCGGCCAAGCAGCACGAATATATCTCCTTTTTCAAGTAATAATGaaa                >  2:665768/1‑140 (MQ=255)
                                                                                                                       ttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAAc               >  2:657830/1‑140 (MQ=255)
                                                                                                                              cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                   <  2:861535/65‑1 (MQ=255)
                                                                                                                              cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                   >  1:861535/1‑65 (MQ=255)
                                                                                                                              cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAGTTCTCCTTTTTCAATTGTTAATGAAACATCtttt        >  2:1346170/1‑140 (MQ=255)
                                                                                                                                    cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt  <  1:2610478/138‑1 (MQ=255)
                                                                                                                                    cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt  <  1:777740/138‑1 (MQ=255)
                                                                                                                                    cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt  <  1:2396850/138‑1 (MQ=255)
                                                                                                                                    cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt  <  1:1777305/138‑1 (MQ=255)
                                                                                                                                    cATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACt  >  2:1640985/1‑140 (MQ=255)
                                                                                                                                    cATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACt  <  1:2622912/140‑1 (MQ=255)
                                                                                                                                    cATCAAACAAAATCGTCACGCTGTCGGCGAAAATGATTTGCTTGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAGTTCTGCTTTTGCAATTGTTAATGAATCATCTTTTAATACt  >  2:1942879/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                    
CATCAAATTTCGGATAAATTCTTCTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACT  >  NZ_CP034943/2920916‑2921187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: